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  • 1 Shahed University, Iran
  • | 2 Shahed University, Iran
  • | 3 National Research Center on Genetic Engineering and Biotechnology, Iran
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Introduction

This study aimed to determine the effects of methyl jasmonate (MJ) combined with putrescine as eco-friendly elicitors on secondary metabolism and gene expression of alkaloid biosynthetic pathway in Catharanthus roseus in vitro-propagated shoots.

Methods

The expression of mitogen-activated protein kinase 3 and the transcription factor, octadecanoid-responsive Catharanthus AP2-domain3, upstream of plant alkaloids’ biosynthetic pathway, and of key genes in the pathway (CrPRX1, STR, DAT, and GS) are investigated as well using qRt-PCR. Antioxidant enzyme (superoxide dismutase, peroxidase, and catalase) activities and non-enzymatic antioxidants (phenolics, flavonoids, and carotenoids) contents have studied to determine the stress levels of the plant by spectrophotometer.

Results

Results showed increased contents of non-enzymatic antioxidants after 4–8 hr and enzymatic antioxidants activities after 24 hr. Alkaloids contents increased mostly after 1 week. The investigated signaling genes upregulated after 8 hr and biosynthetic genes after 24 hr of treatments. Combined treatments had more positive effects on gene expression levels, antioxidant responses, and secondary metabolite production than MJ individually.

Discussion

Increased effects of combined elicitor on genes expression may be due to cross talks between their signaling pathways. Combination of MJ and putrescine can be used as an eco-friendly elicitor for enhancing the production of economically important alkaloids in C. roseus.

Supplementary Materials

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Senior Editors

Editor-in-Chief: Miklósi, Ádám

Managing Editor: Molnár, Csaba

Editorial Board

Maász, Gábor - Hungarian Academy of Sciences, Centre for Ecological Research
Barina, Zoltán - Hungarian Natural History Museum, Department of Botany
Pongrácz, Péter - Eötvös Loránd University, Department of Ethology
Gábriel, Róbert - University of Pécs, Szentágothai Research Centre
Vágvölgyi, Csaba - University of Szeged, Department of Microbiology
Hideg, Éva - University of Pécs, Department of Plant Biology
Solti, Ádám - Eötvös Loránd University, Department of Plan Physiology and Molecular Plant Biology
Erős, Tibor - Hungarian Academy of Sciences, Centre for Ecological Research
Székely, Tamás - University of Bath, University of Debrecen
Dobolyi, Árpád - Eötvös Loránd University, Department of Neurobiology and Physiology
Tamás, Andrea - University of Pécs, Department of Anatomy
Kovács, Tibor - Eötvös Loránd University, Department of Genetics
Serfőző, Zoltán - Hungarian Academy of Sciences, Balaton Limnological Institute
Bede-Fazekas, Ákos - Hungarian Academy of Sciences, Centre for Ecological Research
Bugyi, Beáta - University of Pécs, Department of Biophysics
Fugazza, Claudia - Eötvös Loránd University, Department of Ethology
Chmura, Damjan - University of Bielsko-Biala, Institute of Environmental Protection and Engineering
Neugart, Susanne - Leibniz Institute of Vegetable and Ornamental Crops
Contardo-Jara, Valeska - Technical University of Berlin, Institute of Ecology

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Biologia Futura
Language English
Size A4
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Foundation
2019 (1950)
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1
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Founder Magyar Tudományos Akadémia  
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Springer Nature Switzerland AG
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ISSN 2676-8615 (Print)
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