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  • 1 Department of Refrigeration and Livestock Products' Technology, Faculty of Food Science, Szent István University Department of Refrigeration and Livestock Products Technology H-1118 Budapest, Somlói út 14-16. Hungary Budapest
  • | 2 Department of Refrigeration and Livestock Products' Technology, Szent István University H-1118 Budapest, Ménesi út 45. Hungary
  • | 3 Department of Refrigeration and Livestock Products' Technology, Szent István University Ménesi út 45, H-1118 Budapest, Hungary
  • | 4 Department of Refrigeration and Livestock Products Technology, Faculty of Food Science, Szent István University H-1118 Budapest, Ménesi út 43-45. Hungary
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A bioluminescent derivative of Bacillus subtilis containing a plasmid encoding a luxAB fusion under control of a vegetative promoter and gives bioluminescence upon addition of an exogenous long-chain aldehyde has been used as test organism. Its spore populations have been produced and their heat- and radiation survival curves established. Heat-sensitization effect of pre-irradiation of spores was proven not only by colony counting but also with differential scanning calorimetry. Under a linearly programmed temperature increase, the heat destruction of spores surviving 2.5 kGy gamma irradiation resulted in at a few centigrade lower temperature than that of untreated spores. Heat denaturation endotherms in the DSC-thermogram of irradiated spores were shifted to lower temperatures as well. Comparative turbidimetric, luminometric and phase-contrast microscopic studies of untreated, heat-treated and irradiated spore populations showed that the kinetics of germination and the light emission during germination of radiation-inactivated spores were the same as those of untreated spores, revealing that the pre-formed luciferase enzyme packaged into the spores during sporulation remained intact after an irradiation dose causing 90% decrease in number of colony forming spores. Therefore, in contrast to heat-treated spores, the initial bioluminescence reading upon germination of irradiated spores does not reflect the viable count of their population.

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