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  • 1 B. Johan National Center for Epidemiology Gyáli út 2-6, H-1097 Budapest, Hungary
  • 2 B. Johan National Center for Epidemiology Gyáli út 2-6, H-1097 Budapest, Hungary
  • 3 B. Johan National Center for Epidemiology Gyáli út 2-6, H-1097 Budapest, Hungary
  • 4 B. Johan National Center for Epidemiology Gyáli út 2-6, H-1097 Budapest, Hungary
  • 5 B. Johan National Center for Epidemiology Gyáli út 2-6, H-1097 Budapest, Hungary
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An account is given using typing methods and detection of virulence genes of different serotypes of Escherichia coli isolated in Hungary. By hybridization using SLT-I and SLT-II probes and PCR method using stx1-2, eae and ehx primers we could differentiate O157 strains of different serotypes into eight  (stx, eae, ehxA positive; stx, eae positive; stx, ehxA positive; stx positive; eae, ehxA positive; eae positive; ehxA positive; stx, eae, ehxA negative) types. The discriminatory power of phage typing proves to be much higher than that of the plasmid profile. RAPD typing with different primers could confirm or exclude the subtypes identity of the isolated E. coli O157 serotypes. Escherichia coli O157:HNM isolates could be sorted in six different phage types and six different RAPD types with ERIC-1, in five RAPD types with ERIC-2 and in seven types with M13 primers. Escherichia coli O157:H7 showed six different phage types and three RAPD types with ERIC-1 and ERIC-2 and five types with M13 primers. According to our results the standard PFGE protocol [32] gives the opportunity to differentiate epidemiologically independent but evolutionary related or unrelated isolates, but the practical value of PFGE method for epidemiological purposes must be confirmed by other or more restriction enzymes or using an other protocol. Summarizing our results we suggest the use of phage and RAPD typing and in doubtful cases the PFGE method.

  • Riley, L. W., Remis, R. S., Helgerson, S. D. et al.: Hemorrhagic colitis associated with rare Escherichia coli serotype. N Engl J Med 308, 681 (1983).

    'Hemorrhagic colitis associated with rare Escherichia coli serotype. ' () 308 N Engl J Med : 681.

    • Search Google Scholar
  • Newland, J. W., Neill, R. J.: DNA probes for Shiga-like toxins I. and II. and for toxin-converting bacteriophages. J Clin Microbiol 26, 1292 (1988).

    'DNA probes for Shiga-like toxins I. and II. and for toxin-converting bacteriophages. ' () 26 J Clin Microbiol : 1292.

    • Search Google Scholar
  • Michino, H., Araki, K., Minami, S., Nakayama, T., Ejima, Y., Hiroe, K. et al.: Recent outbreaks of infections caused by Escherichia coli O 157:H7 in Japan. In: Kaper, J. B., O'Brien, A. D. (eds): Escherichia coli O157:H7 and other Shiga-toxin-producing E. coli strains. American Society for Microbiology, Washington, DC, 1998, pp. 73-81.

    Escherichia coli O157:H7 and other Shiga-toxin-producing E. coli strains. , () 73 -81.

    • Search Google Scholar
  • Scotland, S. M., Rowe, B., Smith, H. R., Willshaw, G. A., Gross, R. J.: Vero cytotoxin-producing strains of Escherichia coli from children with hemolytic uremic syndrome and their detection by specific DNA probes. J Med Microbiol 25, 237 (1988).

    'Vero cytotoxin-producing strains of Escherichia coli from children with hemolytic uremic syndrome and their detection by specific DNA probes. ' () 25 J Med Microbiol : 237.

    • Search Google Scholar
  • Karmali, M.: Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev 2, 15 (1989).

    'Infection by verocytotoxin-producing Escherichia coli. ' () 2 Clin Microbiol Rev : 15.

  • Karch, H., Böhm, H., Schmidt, H., Gunzer, F., Aleksic, S., Heesemann, J.: Clonal structure and pathogenicity of Shiga-like toxin producing, sorbitol-fermenting Escherichia coli O157:H. J Clin Microbiol 31, 1200 (1993).

    'Clonal structure and pathogenicity of Shiga-like toxin producing, sorbitol-fermenting Escherichia coli O157:H. ' () 31 J Clin Microbiol : 1200.

    • Search Google Scholar
  • Herpay, M., Czirók, É., Pádszti, J., Gadó, I., Milch, H.: Verocytotoxin-producing Escherichia coli isolates in Hungary. 2nd International Symposium and Workshop on "Verocytotoxin (Shiga-like toxin)-producing Escherichia coli infections". Bergamo, 1994, p. 40.

    Verocytotoxin-producing Escherichia coli isolates in Hungary. 2nd International Symposium and Workshop on "Verocytotoxin (Shiga-like toxin)-producing Escherichia coli infections". , () 40.

    • Search Google Scholar
  • Herpay, M., Czirók, É., Nyomárkai, I., Mitro, E., Pászti, J.: Verocytotoxin-producing Escherichia coli isolates in Hungary. Alpe Adria Microbiology Journal 3, 205 (1994).

    'Verocytotoxin-producing Escherichia coli isolates in Hungary. ' () 3 Alpe Adria Microbiology Journal : 205.

    • Search Google Scholar
  • Czirók, É., Herpay, M.: National surveillance of VTEC infection. Notiziario dell' Instituto Superiore du Sanita, EVC news 1-2 (1995).

    'National surveillance of VTEC infection. ' () 1-2 Notiziario dell' Instituto Superiore du Sanita, EVC news .

    • Search Google Scholar
  • Milch, H., Pászti, J., Gadó, I., Tóth, I., Czirók, É., Herpay, M.: Phage typing, plasmid profile determination and genetic probing for Shiga-like-toxins of Escherichia coli O157. 2nd International Symposium and Workshop on "Verocytotoxin (Shiga-like toxin)-producing Escherichia coli infections". Bergamo, 1994, p. 39.

  • Milch, H., Pászti, J., Gadó, I., Tóth, I., Czirók, É., Herpay, M.: Escherichia coli O157. komplex tipizálása és verotoxin génjének kimutatása DNS próbával (in Hungarian). Egészségtudomány 39, 260 (1995).

    'Escherichia coli O157. komplex tipizálása és verotoxin génjének kimutatása DNS próbával (in Hungarian). ' () 39 Egészségtudomány : 260.

    • Search Google Scholar
  • Czirók, É., Marton, A., Csík, M., Solt, M.: Virulence factors of plasmid profile detertmination and genetic probing for Shiga-like toxins of Escherichia coli I. Mannose-resistant haemagglutinating capacity is associated with serogroup but not with site of infection. Acta Microbiol Hung 31, 187 (1984).

    'Virulence factors of plasmid profile detertmination and genetic probing for Shiga-like toxins of Escherichia coli I. Mannose-resistant haemagglutinating capacity is associated with serogroup but not with site of infection. ' () 31 Acta Microbiol Hung : 187.

    • Search Google Scholar
  • Khakhria, R., Duck, D., Lior, H.: Extended phage-typing scheme for Escherichia coli O157:H7. Epidemiol Infect 105, 511 (1990).

    'Extended phage-typing scheme for Escherichia coli O157:H7. ' () 105 Epidemiol Infect : 511.

    • Search Google Scholar
  • Milch, H.: Phage typing of Escherichia coli. In: Bergan, T., Norris, J. R. (eds): Methods in Microbiology. Academic Press, London, 1978, p. 11.

    Methods in Microbiology. , () 11.

  • Lafont, J. P., Dho, M., D'Hauteville, H. M., Bree, A., Sansonetti, Ph.: Presence and expression of aerobactin genes in virulent avian strains of Escherichia coli. Infect Immun 55, 193 (1987).

    'Presence and expression of aerobactin genes in virulent avian strains of Escherichia coli. ' () 55 Infect Immun : 193.

    • Search Google Scholar
  • Kado, C. L., Liu, S. T.: Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 145, 1365 (1981).

    'Rapid procedure for detection and isolation of large and small plasmids. ' () 145 J Bacteriol : 1365.

    • Search Google Scholar
  • Griffin, P. M., Tauxe, R. V.: The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli and the associated hemolytic uremic syndrome. Epidemiologic Reviews 13, 60 (1991).

    'The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli and the associated hemolytic uremic syndrome. ' () 13 Epidemiologic Reviews : 60.

    • Search Google Scholar
  • Tóth, I., Karcagi, V., Nagy, B., Gadó, I., Milch, H.: Examination of verocytotoxin-producing capacity and determination of presence of Shiga-like toxin genes in human Escherichia coli isolates. Acta Microbiol Immunol Hung 41, 259 (1994).

    'Examination of verocytotoxin-producing capacity and determination of presence of Shiga-like toxin genes in human Escherichia coli isolates. ' () 41 Acta Microbiol Immunol Hung : 259.

    • Search Google Scholar
  • Meyers, J. A., Sanchez, D., Elwell, L. P., Falkow, S.: Simple agarose gel electrophoretic method for the identification and characterization of deoxy-ribonucleic acid. J. Bacteriol 127, 1529 (1976).

    'Simple agarose gel electrophoretic method for the identification and characterization of deoxy-ribonucleic acid. ' () 127 J. Bacteriol : 1529.

    • Search Google Scholar
  • Baldini, M. M., Nataro, J. P., Kaper, J. B.: Localization of determinant for HEp-2 adherence by enteropathogenic Escherichia coli. J Infect Immun 52, 334 (1986).

    'Localization of determinant for HEp-2 adherence by enteropathogenic Escherichia coli. ' () 52 J Infect Immun : 334.

    • Search Google Scholar
  • Newland, J. W., Strockbine, N. A., Miller, S. F., O'Brien, A. D., Holmes, R. K.: Cloning of Shiga-like toxin structural genes from a toxin converting phage of Escherichia coli. Science 230, 179 (1985).

    'Cloning of Shiga-like toxin structural genes from a toxin converting phage of Escherichia coli. ' () 230 Science : 179.

    • Search Google Scholar
  • Willshow, G. A., Smith, H. R., Scotland, S. M., Field, A. M., Rowe, B.: Heterogeneity of Escherichia coli phages encoding verocytotoxin: comparison of cloned sequences determining VT1 and VT2 and development of specific gene probes. J Gen Microbiol 133, 1309 (1987).

    'Heterogeneity of Escherichia coli phages encoding verocytotoxin: comparison of cloned sequences determining VT1 and VT2 and development of specific gene probes. ' () 133 J Gen Microbiol : 1309.

    • Search Google Scholar
  • Sethabutr, O., Echeverria, P., Hanchalay, S., Taylor, D. N., Lekosonboon, U.: A non-radioactive DNA probe to identify Shigella and enteroinvasive Escherichia coli in stools of children with diarrhoea. Lancet 2, 1095 (1985).

    'A non-radioactive DNA probe to identify Shigella and enteroinvasive Escherichia coli in stools of children with diarrhoea. ' () 2 Lancet : 1095.

    • Search Google Scholar
  • Fratamico, P. M., Sackitey, S. K., Wiedemann, M., Ming Yi Deng: Detection of Escherichia coli O157:H7 by multiplex PCR. J Clin Microbiol 33, 2188 (1995).

    'Detection of Escherichia coli O157:H7 by multiplex PCR. ' () 33 J Clin Microbiol : 2188.

    • Search Google Scholar
  • Karch, H., Meyer, T.: Single primer pair for amplifying segments of distinct Shiga-like toxin genes by polymerase chain reaction. J Clin Microbiol 27, 2751 (1989).

    'Single primer pair for amplifying segments of distinct Shiga-like toxin genes by polymerase chain reaction. ' () 27 J Clin Microbiol : 2751.

    • Search Google Scholar
  • Gannon, V. P. J., Rashed, M., King, R. K., Thomas, E. J. G.: Detection and characterization of eae gene of Shiga-like toxin producing Escherichia coli using polymerase chain reaction. J Clin Microbiol 31, 1268 (1993).

    'Detection and characterization of eae gene of Shiga-like toxin producing Escherichia coli using polymerase chain reaction. ' () 31 J Clin Microbiol : 1268.

    • Search Google Scholar
  • Gadó, I., Ruzsics, Zs., Tóth, I., Király, M., Milch, H.: Partial inhibition of amplification by primers of EHEC genes. Acta Microbiol Immunol Hung 45, 239 (1998).

    'Partial inhibition of amplification by primers of EHEC genes. ' () 45 Acta Microbiol Immunol Hung : 239.

    • Search Google Scholar
  • Birch, M., Denning, D. W., Law, D.: Rapid genotyping of Escherichia coli O157 isolates by random amplification of polymorphic DNA. Eur J Clin Microbiol Infect Dis 15, 297 (1996).

    'Rapid genotyping of Escherichia coli O157 isolates by random amplification of polymorphic DNA. ' () 15 Eur J Clin Microbiol Infect Dis : 297.

    • Search Google Scholar
  • van Belkum, A., Melchers, W. J, de Pauw B. E.: Genotypic characterization of sequential Candida albicans isolates from fluconazide-treated neutropenic patients. J Infect Dis 169, 1062 (1994).

    'Genotypic characterization of sequential Candida albicans isolates from fluconazide-treated neutropenic patients. ' () 169 J Infect Dis : 1062.

    • Search Google Scholar
  • Schonian, G., Meusel, O., Tietz, H. J., Meyer, W., Graser, Y., Tausch, I., Presber, W., Mitchell, T. G.: Identification of clinical strains of Candida albicans by DNA fingerprinting with polymerase chain reaction. Mycoses 36, 171 (1993).

    'Identification of clinical strains of Candida albicans by DNA fingerprinting with polymerase chain reaction. ' () 36 Mycoses : 171.

    • Search Google Scholar
  • Gautom, R. K.: Rapid pulsed field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day. J Clin Microbiol 35, 2977 (1977).

    'Rapid pulsed field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day. ' () 35 J Clin Microbiol : 2977.

    • Search Google Scholar
  • Foodborne and Diarrheal Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention. 196. Standardized molecular subtyping of Escherichia coli O157:H7 by Pulsed-field gel electrophoresis: a training manual. Disease control and prevention, Atlanta, Ga.

  • Bygraves, J. A., Maiden, M. C. J.: Analysis of the clonal relationships between strains of Neisseria meningitidis by pulsed-field gel electrophoresis. J Gen Microbiol 138, 523 (1992).

    'Analysis of the clonal relationships between strains of Neisseria meningitidis by pulsed-field gel electrophoresis. ' () 138 J Gen Microbiol : 523.

    • Search Google Scholar
  • Struelens, M. J. and Members of the European Study Group on Epidemiological Markers (ESGEM) and of the European Society for Clinical Microbiology and Infectious Diseases (ESCMID). Consensus guidelines for appropriate use of microbial epidemiologic typing systems. Clin Microbiol and Infection 2, 2-11 (1996).

  • Izumiya H., Terajima, J., Wada, A., Inagaki, Y., Itho, K., Tamura, K., Watanabe, H.: Molecular typing of enterophoresis. J Clin Microbiol 35, 1675 (1997).

    'Molecular typing of enterophoresis. ' () 35 J Clin Microbiol : 1675.

  • Smith, H. R., Willshow, G. A., Thomas, A., Rowe, B.: Applications of DNA probes for Vero cytotoxin-producing Escherichia coli. J Hosp Infect 18 (Suppl A), 438 (1991).

    'Applications of DNA probes for Vero cytotoxin-producing Escherichia coli. ' () 18 J Hosp Infect : 438.

    • Search Google Scholar
  • Thomas, A., Smith, H. R., Rowe, B.: Use of digoxigenin labelled oligonucleotide DNA probes for VT2 and VT2 human variant genes to differentiate vero-cytotoxin-producing Escherichia coli strains of serogroup O157. J Clin Microbiol 31, 1700 (1993).

    'Use of digoxigenin labelled oligonucleotide DNA probes for VT2 and VT2 human variant genes to differentiate vero-cytotoxin-producing Escherichia coli strains of serogroup O157. ' () 31 J Clin Microbiol : 1700.

    • Search Google Scholar
  • Arbeit R. D., Arthur, M., Dunn, R., Kim, C, Selander, R. K., Goldstein, R.: Resolution of recent evolutionary divergence among Escherichia coli from related lineages: the application of pulsed field electrophoresis to molecular epidemiology. J Infect Dis 161, 230 (1990).

    'Resolution of recent evolutionary divergence among Escherichia coli from related lineages: the application of pulsed field electrophoresis to molecular epidemiology. ' () 161 J Infect Dis : 230.

    • Search Google Scholar
  • Böhm, H. Karch, H.: DNA fingerprinting of Escherichia coli O157:H7 strains by pulsed-field gel electrophoresis. J Clin Microbiol 30, 2169 (1992).

    'DNA fingerprinting of Escherichia coli O157:H7 strains by pulsed-field gel electrophoresis. ' () 30 J Clin Microbiol : 2169.

    • Search Google Scholar
  • Willshaw, G. A., Scotland, S. M., Smith, H. R., Cheasty T., Thomas, A., Rowe, B.: Hybridization of strains of Escherichia coli O157 with probes derived from the eaeA gene of enteropathogenic E. coli and the eaeA homolog from a Vero cytotoxin-producing strain of E. coli O157. J Clin Microbiol 32, 897 (1994).

    'Hybridization of strains of Escherichia coli O157 with probes derived from the eaeA gene of enteropathogenic E. coli and the eaeA homolog from a Vero cytotoxin-producing strain of E. coli O157. ' () 32 J Clin Microbiol : 897.

    • Search Google Scholar
  • Frost, J. A., Smith, H. R., Willshow, G. A., Scotland, S. M., Gross, R. J., Rowe, B.: Phage-typing of vero-cytotoxin (VT) producing Escherichia coli O157 isolated in the United Kingdom. Epidem Inf 103, 73 (1989).

    'Phage-typing of vero-cytotoxin (VT) producing Escherichia coli O157 isolated in the United Kingdom. ' () 103 Epidem Inf : 73.

    • Search Google Scholar
  • van Belkum, A.: DNA fingerprinting of medically important microorganisms by use of PCR. Clin Microbiol Rev 7, 174 (1994).

    'DNA fingerprinting of medically important microorganisms by use of PCR. ' () 7 Clin Microbiol Rev : 174.

    • Search Google Scholar
  • Seward, R. J., Ehrenstein, B., Grundmann, H. J., Towner, K. J.: Direct comparison of two commercially available computer programs for analysing DNA fingerprinting gels. J Med Microbiol 46, 314 (1997).

    'Direct comparison of two commercially available computer programs for analysing DNA fingerprinting gels. ' () 46 J Med Microbiol : 314.

    • Search Google Scholar
  • Renders, N., Römling, U., Verbrugh, H., van Belkum, A.: Comparative typing of Pseudomonas aeruginosa by random amplification of polymorphic DNA or pulsed-field gel electrophoresis of DNA macrorestriction fragments. J Clin Microbiol 34, 3190 (1996).

    'Comparative typing of Pseudomonas aeruginosa by random amplification of polymorphic DNA or pulsed-field gel electrophoresis of DNA macrorestriction fragments. ' () 34 J Clin Microbiol : 3190.

    • Search Google Scholar
  • Preston, M. A., Johnson, W., Khakhria, R., Borczyk, A.: Epidemiologic subtyping of Escherichia coli serogroup O157 strains in Ontario by phage typing and pulsed-field gel electrophoresis. J Clin Microbiol 38, 2366 (2000).

    'Epidemiologic subtyping of Escherichia coli serogroup O157 strains in Ontario by phage typing and pulsed-field gel electrophoresis. ' () 38 J Clin Microbiol : 2366.

    • Search Google Scholar
  • Bettelheim, K., A., Hormitzky, M. A., Dsjordjevics, S. P., Kuzevski, A.: Antibiotic resistance among verocytotoxigenic Escherichia coli (VTEC) and non-VTEC isolated from domestic animals and humans. J Med Microbiol 52, 155 (2003).

    'Antibiotic resistance among verocytotoxigenic Escherichia coli (VTEC) and non-VTEC isolated from domestic animals and humans. ' () 52 J Med Microbiol : 155.

    • Search Google Scholar
  • Tenover, F. C., Arbeit, R. D., Goering, R. V., Mickelsen, P. A., Murray, B. E., Persing, D. H., Swaminathan, B.: Interpreting chromosomal DNA restriction patterns by pulsed-field gel electrophoresis: Criteria for bacterial strain typing. J Clin Microbiol 33, 2233 (1995).

    'Interpreting chromosomal DNA restriction patterns by pulsed-field gel electrophoresis: Criteria for bacterial strain typing. ' () 33 J Clin Microbiol : 2233.

    • Search Google Scholar
  • Milch, H., Gadó, I., Drin, I., Czirók, É., Herpay, M.: Detection of VTEC using specific DNA probes and complex typing of Escherichia coli O157. Acta Microbiol Immunol Hung 44, 257 (1997).

    'Detection of VTEC using specific DNA probes and complex typing of Escherichia coli O157. ' () 44 Acta Microbiol Immunol Hung : 257.

    • Search Google Scholar
  • Schroeder, C. M., Meng, J., Zhao, Sh., Deb Roy, C., Torcolini, J., Zhao, C., Mc Dermott, P. F., Wagner, D. D., Walker, R. D., White, D. G.: Antimicrobial resistance of Escherichia coli O26, O103, O111, O128, O145 from animals and humans. Emerging Inf. Dis. 8, 1409 (2002).

    'Antimicrobial resistance of Escherichia coli O26, O103, O111, O128, O145 from animals and humans. ' () 8 Emerging Inf. Dis. : 1409.

    • Search Google Scholar
  • Polotsky, Yu. E., Dragunskaya, E. M., Seliverstova, V. G., Avdeeva, T. A., Chakhutinskaya, M. G., Kétyi, L, Vertényi, A., Ralovich, B., Emödy, L., Málovics, I., Safonova, N. V., Snigirevskaya, E. S., Karyagina, E. I.: Pathogenic effect of enterotoxigenic Escherichia coli and Escherichia coli causing infantile diarrhoea. Acta Microbiol Acad Sci Hung 24, 221 (1977).

    'Pathogenic effect of enterotoxigenic Escherichia coli and Escherichia coli causing infantile diarrhoea. ' () 24 Acta Microbiol Acad Sci Hung : 221.

    • Search Google Scholar

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