Acta Microbiologica et Immunologica Hungarica
Volume/Issue:
Volume 51: Issue 1-2
Lead induced modulation of splenic macrophage responses on humoral and cell mediated immunity
Authors: B. Bishayi 1 , M. Sengupta 2 , and Soumya Ghosh 3
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  • 1 Immunology Laboratory, Department of Physiology, University of Calcutta, University College of Science and Technology 92, Acharya Prafulla Chandra Road, Calcutta 700009, India
  • 2 Immunology Laboratory, Department of Physiology, University of Calcutta, University College of Science and Technology 92, Acharya Prafulla Chandra Road, Calcutta 700009, India
  • 3 Immunology Laboratory, Department of Physiology, University of Calcutta, University College of Science and Technology 92, Acharya Prafulla Chandra Road, Calcutta 700009, India
DOI:
https://doi.org/10.1556/amicr.51.2004.1-2.2
Page Count:
31–45
Publication Date:
01 May 2004
Online Publication Date:
22 Jul 2005
Article Category:
Research Article
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The heavy metal lead is an environmental toxic material that can induce pathophysiological changes in many organ systems. Previous studies have shown the effects of lead exposure on immune cells in different experimental animals, however, the mechanism of their influence on the immune system is unclear. We reported that in vivo lead exposure inhibits phagocytosis, nitric oxide release, induces DNA fragmentation suggesting the apoptotic death of the target cell. We have also presented evidence that inhibition of macrophage functional responses implicated alteration of humoral and cell mediated immunity. In vivo exposure to lead acetate alters the phagocytic capacity of splenic macrophages as evident from the reduction of phagocytic index of control from 19,792±1385.69 to 8893 ± 893 in the treated group. The amount of nitric oxide released by the control cell 2.25 ± 0.125 µM is also reduced to 1.9375 ± 0.0625 µM upon in vivo lead treatment. Functional integrity of the target cell is also decreased after lead exposure as obtained from the percentage of DNA fragmentation. Control group shows 33.29 ± 0.11% of fragmented DNA, which is enhanced to 42.43 ± 0.725% following the lead treatment. A greater percentage of DNA fragmentation upon lead treatment probably indicating that the heavy metal induces apoptosis. The humoral immune response is also altered after lead exposure as indicated by the decrease of the antibody titre in control group from 1:2048 to1:128 in the treated group.  From the DTH reaction, it was observed that the mean diameter of swollen foot pad of control mice is 0.329 ± 0.15 cm and that of lead treated mice is 0.274 ± 0.056 cm. It can, therefore, be suggested that lead inhibits normal functional activities of splenic leukocytes, particularly phagocytosis and also affects the functional integrity of cells by inducing DNA fragmentation. The study may demonstrate the usefulness of investigation of humoral immune system and leukocyte functions as sensitive parameters in detecting the effects of lead toxicity.

  • Kowolenko, M., Tracy, L., Mudzinski, S., Lawrence, D. A.: Effect of lead on macrophage function. J Lcukoc Biol 43(4), 357-364 (1988).

    'Effect of lead on macrophage function. ' () 43 J Lcukoc Biol : 357 -364.

  • Waalkes, M. P., In: Goyer, R. A., Klaasen, C. D., Waalkes, M. P. (eds): Metal Toxicology. Academic Press, pp. 54-56 (1995).

    Metal Toxicology. , () 54 -56.

  • Timbrell, J. A.: Biochemical mechanism of toxicity: specific examples. In: Principles of Biochemical Toxicology. 2nd ed. Taylor & Francis, London, pp. 374-377 (1991).

    Principles of Biochemical Toxicology. , () 374 -377.

  • Winneke, G.: Behavioural Toxicology. In: Duffers, J. H., Howard, G. J. (eds): Fundamental Toxicology for Chemists. The Royal Society of Chemistry, United Kingdom, pp. 175-176 (1996).

    Fundamental Toxicology for Chemists. , () 175 -176.

  • Sikorsi, E. E., Burns, L. A., Stern, M. L., Luster, M. I., Munson, A. E.: Splenic cell targets in gallium, arsenic induced suppression of primary antibody response. Toxicol Appl Pharmacol 110, 179-192 (1991).

    'Splenic cell targets in gallium, arsenic induced suppression of primary antibody response. ' () 110 Toxicol Appl Pharmacol : 179 -192.

    • Search Google Scholar

  • Czuprynski, C. J., Hensen, P. M., Campbell, P. M.: Killing of Listeria monocytogenes by inflammatory neutrophils and mononuclear phagocytes from immune and nonimmune mice. J Leucoc Biol 35, 192-208 (1984).

    'Killing of Listeria monocytogenes by inflammatory neutrophils and mononuclear phagocytes from immune and nonimmune mice. ' () 35 J Leucoc Biol : 192 -208.

    • Search Google Scholar

  • Klaassen, C. D.: Heavy metals and heavy metal antagonists. In: Gilman, A. G. et al. (eds): The Pharmacological Basis of Therapeutics. McGrawHill, New York, pp. 1650-1653 (1996).

    The Pharmacological Basis of Therapeutics. , () 1650 -1653.

  • Laschi, A., Eyrand, A., Morisset, D., Sanou, A., Tachon, P., Veysseyre, C., Descotes, J.: Influence of the heavy metals on the resistance of mice toward infection. Immunopharmacol Immunotoxicol 9(2), 235-241 (1987).

    'Influence of the heavy metals on the resistance of mice toward infection. ' () 9 Immunopharmacol Immunotoxicol : 235 -241.

    • Search Google Scholar

  • Lawrence, D. A.: In vivo and in vitro effects of lead on humoral and cell mediated immunity. Infect Immun 31(1), 143 (1981).

    'In vivo and in vitro effects of lead on humoral and cell mediated immunity. ' () 31 Infect Immun : 143.

    • Search Google Scholar

  • Miller, T. F., Golemboski, K. A., Ma, R. S., Burn, T., Sanders, F. S., Dietert, R. R.: Development exposure to lead causes persistent immunotoxicity in Fischer 344 rats. Toxicol Sci 42(2), 129-135 (1998).

    'Development exposure to lead causes persistent immunotoxicity in Fischer 344 rats. ' () 42 Toxicol Sci : 129 -135.

    • Search Google Scholar

  • Robbiano, L., Carrozino, R., Puglia, C. P., Corbu, C., Brambilla, G.: Correlation between induction of DNA fragmentation and micronuclei formation in kidney cells from rats and humans and their specific carcinogenic activity. Toxicol Appl Pharmacol 162(2), 153-159 (1999).

    'Correlation between induction of DNA fragmentation and micronuclei formation in kidney cells from rats and humans and their specific carcinogenic activity. ' () 162 Toxicol Appl Pharmacol : 153 -159.

    • Search Google Scholar

  • Smith, K. I., Lawrence, D. A.: Immunomodulation of in vitro antigen presentation by cations. Toxicol Appl Pharmacol 96(3), 746-484 (1988).

    'Immunomodulation of in vitro antigen presentation by cations. ' () 96 Toxicol Appl Pharmacol : 746 -484.

    • Search Google Scholar

  • Tian, L., Lawrence D. A.: Lead inhibits nitric oxide production in vitro by murine splenic macrophages. Toxicol Appl Pharmacol 137(1), 163-163 (1995).

    'Lead inhibits nitric oxide production in vitro by murine splenic macrophages. ' () 137 Toxicol Appl Pharmacol : 163 -163.

    • Search Google Scholar

  • Valentino, M., Governa, M., Marchiseppe, I., Visona, I.: Effect of lead on polymorphonuclear leucocyte function in occupationally exposed workers. Arch Toxicol 65(8), 685-688 (1991).

    'Effect of lead on polymorphonuclear leucocyte function in occupationally exposed workers. ' () 65 Arch Toxicol : 685 -688.

    • Search Google Scholar

  • Greer, W. I., Kaplan, J. G.: DNA strand breaks in murine lymphocytes: induction of purine and pyrimidine analogues. Biochem Biophys Res Commun 115(3), 835-840 (1983).

    'DNA strand breaks in murine lymphocytes: induction of purine and pyrimidine analogues. ' () 115 Biochem Biophys Res Commun : 835 -840.

    • Search Google Scholar

  • Exon, J-H., Talcott, P. A., Koller, I. D.: Effects of lead, polychlorinated biphenyls and cyclophosphamide on rat natural killer cells, interleukin-2 and antibody synthesis. Fundamentals of Applied Toxicology 5(1), 158-164 (1985).

    'Effects of lead, polychlorinated biphenyls and cyclophosphamide on rat natural killer cells, interleukin-2 and antibody synthesis. ' () 5 Fundamentals of Applied Toxicology : 158 -164.

    • Search Google Scholar

  • Sasaki, S., Miura, T., Nishikawa, S., Yamada, K., Hirasue, M., Nakane, A.: Protective role of nitric oxide in S. aureus infection in mice. Infect Immun 66(3), 1017-1022 (1998).

    'Protective role of nitric oxide in S. aureus infection in mice. ' () 66 Infect Immun : 1017 -1022.

    • Search Google Scholar

  • Ochiai, T., Fukushima, K., Ochiai, K.: Butyric acid induced apoptosis of murine thymocytes, splenic T cells and Human Jurkat T-cells. Infect Immun 65(1), 35-41 (1997).

    'Butyric acid induced apoptosis of murine thymocytes, splenic T cells and Human Jurkat T-cells. ' () 65 Infect Immun : 35 -41.

    • Search Google Scholar

  • Agglutination In: Kabat, E. A., Mayer, M. M. (eds): Experimental Immunochemistry. 2nd ed. pp. 97-132 (1967).

    Experimental Immunochemistry. , () 97 -132.

  • Papadimitaiou, C., Hann, H., Naher, H., Kautmann, S. H.: Cellular immune response to sheep erythrocytes: Interrelationship between proliferation of popliteal lymphnode cells and foot pad swelling. Immunobiology 1964, 361-369 (1983).

    'Cellular immune response to sheep erythrocytes: Interrelationship between proliferation of popliteal lymphnode cells and foot pad swelling. ' () 1964 Immunobiology : 361 -369.

    • Search Google Scholar

  • Tian, L., Lawrence, D. A.: Metal induced modulation of nitric oxide production in vitro by murine macrophages: Lead, Nickel and Cobalt utilize different mechanisms. Toxicol Appl Pharmacol 141, 540-547 (1996).

    'Metal induced modulation of nitric oxide production in vitro by murine macrophages: Lead, Nickel and Cobalt utilize different mechanisms. ' () 141 Toxicol Appl Pharmacol : 540 -547.

    • Search Google Scholar

  • Singh, J., Pritchand, D. E., Carlisle, D. L., Maclean, J. A., Montaser, A.: Internalization of carcinogenic lead chromate particles by cultured normal human epithelial cells: formation of intracellular lead inclusion bodies and induction of apoptosis. Toxicol Appl Pharmacol 161, 240-248 (1999).

    'Internalization of carcinogenic lead chromate particles by cultured normal human epithelial cells: formation of intracellular lead inclusion bodies and induction of apoptosis. ' () 161 Toxicol Appl Pharmacol : 240 -248.

    • Search Google Scholar

  • Zelikoff, J. T., Li, J. H., Hartwig, A., Wang, X. W., Costa, M., Rossman, T. G.: Genetic toxicology of lead compounds. Carcinogenesis 9, 1727-1732 (1988).

    'Genetic toxicology of lead compounds. ' () 9 Carcinogenesis : 1727 -1732.

  • Schlick, E., Kamran, M. A., Friedberg, K. O.: Lead in blood and tissues of mice after administration of low lead doses. Archieves Toxicol 46, 221-232 (1980).

    'Lead in blood and tissues of mice after administration of low lead doses. ' () 46 Archieves Toxicol : 221 -232.

    • Search Google Scholar

  • Baginski, B.: Effect of lead and cadmium on the viability and phagocytosis of human polymorphonuclear leucocytes (Articles in German). Zentralbl Bacteriol Microbial Hyg B 181, 461-468 (1985).

    'Effect of lead and cadmium on the viability and phagocytosis of human polymorphonuclear leucocytes (Articles in German). ' () 181 Zentralbl Bacteriol Microbial Hyg B : 461 -468.

    • Search Google Scholar

  • Valentino, M., Governa, M., Marchiseppe, I., Visona, I.: Effects of lead on polymorphonuclear leucocyte functions in occupationally exposed workers. Archives Toxicol 65, 685-688 (1991).

    'Effects of lead on polymorphonuclear leucocyte functions in occupationally exposed workers. ' () 65 Archives Toxicol : 685 -688.

    • Search Google Scholar
Cover Acta Microbiologica et Immunologica Hungarica
Acta Microbiologica et Immunologica Hungarica A Quarterly of the Hungarian Academy of Sciences
Print ISSN:
1217-8950
Online ISSN:
1588-2640
Keywords:
heavy metal intoxication; heavy metal intoxication; splenic macrophages; immune function

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