View More View Less
  • 1 Department of Medical Microbiology and Immunobiology, University of Szeged Szeged, Hungary
  • | 2 Department of Rheumatology, County Hospital Kecskemét, Hungary
  • | 3 Department of Medical Microbiology and Immunobiology, University of Szeged P. O. Box 427, Dóm tér 10, H-6720 Szeged, Hungary
  • | 4 Department of Rheumatology, County Hospital Kecskemét, Hungary
  • | 5 Department of Medical Microbiology and Immunobiology, Albert Szent-Györgyi Medical Centre, Faculty of Medicine, University of Szeged Dóm tér 10, H-6720 Szeged, Hungary
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $784.00

Objectives: The aim of this work was to investigate the prevalence of TNF-a -308 polymorphism among the 29 members of a family with RA and the association between the MHC-linked biallelic HSP70-2 gene and the TNF-a polymorphism. Five of the members with RA were diagnosed by using the revised 1987 ACR criteria, and 1 member suffered from SLE. Methods: The variations in the TNF-a and the HSP70-2 genotypes were analyzed by PCR-RFLP, using NcoI and PstI restriction enzymes. Results: Two of the 29 members were homozygotes for allele A, 18 were heterozygotes (TNF A/G) and 9 of them were homozygotes for allele G. Nineteen of the 29 were heterozygotes for HSP70-2 (A/G), 10 of them were homozygotes for the G allele, and none were homozygotes for allele A. Four of the 5 the RA patients carried the A allele for TNF-a all 5 were heterozygotes for HSP70-2 genotypes. Conclusion: The carriage of the A allele for TNF-a of -308 SNP in 4 of the 5 RA patients, and the high prevalence (68.0%) of TNF A allele carriers in this family confirms the important role of this candidate gene in the pathomechanism of RA, and might be of prognostic value for future clinical observations. Further, to test for association a much larger set of genetically independent patients and controls is needed. 

  • Feldmann, M., Brennan, F. M., Elliott, M. J., Williams, R. O., Maini, R. N.: TNFα is an effective therapeutic target in rheumatoid arthritis. Ann NY Acad Sci 766, 272 (1995).

    'TNFα is an effective therapeutic target in rheumatoid arthritis. ' () 766 Ann NY Acad Sci : 272.

    • Search Google Scholar
  • Waldron-Lynch, F., Adams, C., Amos, C., Zhu, D. K., McDermott, M. F., Shanahan, F., Molloy, M. G., O'Gara, F.: Tumour necrosis factor 5' promoter single nucleotide polymorphisms influence susceptibility to rheumatoid arthritis in immunogenetically defined multiplex RA families. Genes Immun 2, 82 (2001).

    'Tumour necrosis factor 5' promoter single nucleotide polymorphisms influence susceptibility to rheumatoid arthritis in immunogenetically defined multiplex RA families. ' () 2 Genes Immun : 82.

    • Search Google Scholar
  • Ruuls, S. R., Sedgwick, J. D.: Unlinking tumor necrosis factor biology from the major histocompatibility complex: lessons from human genetics and animal models. Am J Hum Genet 65, 294 (1999).

    'Unlinking tumor necrosis factor biology from the major histocompatibility complex: lessons from human genetics and animal models. ' () 65 Am J Hum Genet : 294.

    • Search Google Scholar
  • Feldmann, M., Brennan, M. F., Maini, R. N.: Role of cytokines in rheumatoid arthritis. Annu Rev Immunol 14, 397 (1996).

    'Role of cytokines in rheumatoid arthritis. ' () 14 Annu Rev Immunol : 397.

  • Winchester, R.: The molecular basis of susceptibility to rheumatoid arthritis. Adv Immunol 56, 389 (1994).

    'The molecular basis of susceptibility to rheumatoid arthritis. ' () 56 Adv Immunol : 389.

    • Search Google Scholar
  • Gregersen, P. K., Silver, J., Wincester, R. J.: The shared epitope hypothesis. An approach to understanding the molecular genetics of susceptibility to rheumatoid arthritis. Arthritis Rheum 30, 1205 (1987).

    'The shared epitope hypothesis. An approach to understanding the molecular genetics of susceptibility to rheumatoid arthritis. ' () 30 Arthritis Rheum : 1205.

    • Search Google Scholar
  • Auger, I., Escola, J. M., Gorvel, J. P., Roudier, J.: HLA-DR4 and HLA-DR10 motifs that carry susceptibility to rheumatoid arthritis bind 70-kd heat shock proteins. Nature Med 2, 306 (1996).

    'HLA-DR4 and HLA-DR10 motifs that carry susceptibility to rheumatoid arthritis bind 70-kd heat shock proteins. ' () 2 Nature Med : 306.

    • Search Google Scholar
  • Vargas-Alarcon, G., Londono, J. D., Hernandez-Pacheco, G., Gamboa, R., Castillo, E., Pacheco-Tena, C., Cardiel, M. H., Granados, J., Burgos-Vargas, R.: Heat shock protein 70 gene polymorphisms in Mexican patients with spondyloarthropathies. Ann Rheum Dis 61, 48 (2002).

    'Heat shock protein 70 gene polymorphisms in Mexican patients with spondyloarthropathies. ' () 61 Ann Rheum Dis : 48.

    • Search Google Scholar
  • Pociot, F., Rönningen, K. S., Nerup, J.: Polymorphic analysis of the human-linked heat shock protein 70 (HSP70-2) and HSP70-HOM genes in insulin dependent diabetes mellitus. Scand J Immunol 38, 491 (1993).

    'Polymorphic analysis of the human-linked heat shock protein 70 (HSP70-2) and HSP70-HOM genes in insulin dependent diabetes mellitus. ' () 38 Scand J Immunol : 491.

    • Search Google Scholar
  • Wilson, A. G., Giovine, F. S., Blakemore, A. I. F., Duff, G. W.: Single base polymorphism in the human Tumour Necrosis Factor alpha gene detectable by NcoI restriction of PCR product. Hum Mol Gen 1, 353 (1992).

    'Single base polymorphism in the human Tumour Necrosis Factor alpha gene detectable by NcoI restriction of PCR product. ' () 1 Hum Mol Gen : 353.

    • Search Google Scholar
  • Milner, C. M., Campbell, R. D.: Polymorphic analysis of the three MHC-linked HSP70 genes. Immunogenetics 36, 357 (1992).

    'Polymorphic analysis of the three MHC-linked HSP70 genes. ' () 36 Immunogenetics : 357.

    • Search Google Scholar
  • Wilson, A. G., Symons, J. A., McDowel, T. L., McDevitt, H. O. Duff, G. W.: Effect of a polymorphism in the human tumor necrosis factor α promoter on transcriptional activation. Proc Natl Acad Sci 94, 3195 (1997).

    'Effect of a polymorphism in the human tumor necrosis factor α promoter on transcriptional activation. ' () 94 Proc Natl Acad Sci : 3195.

    • Search Google Scholar
  • Louis, E., Franchimont, D., Piron, A., Gevaert, Y., Schaaf-Lafontaine, N., Roland, S., Mahieu, P., Malaise, M., DeGroote, D., Louis, R., Belaiche, J.: Tumor necrosis factor gene polymorphism influences tumor necrosis factor-α production in LPS-stimulated whole blood cell culture in healthy humans. Clin Exp Immunol 113, 401 (1998).

    'Tumor necrosis factor gene polymorphism influences tumor necrosis factor-α production in LPS-stimulated whole blood cell culture in healthy humans. ' () 113 Clin Exp Immunol : 401.

    • Search Google Scholar
  • Bouma, G., Crusius, J. B., Oudkerk, P. M., Kolkman, J. J., von Blomberg, B. M., Kostense, P. J., Giphart, M. J., Schreuder, G. M., Meywissen, S. G., Pena, A. S.: Secretion of tumor necrosis factor α and lymphotoxin α in relation to polymorphism in the TNF genes and HLA-DR alleles. Relevance for inflammatory bowel disease. Scand J Immunol 43, 456 (1996).

    'Secretion of tumor necrosis factor α and lymphotoxin α in relation to polymorphism in the TNF genes and HLA-DR alleles. Relevance for inflammatory bowel disease. ' () 43 Scand J Immunol : 456.

    • Search Google Scholar

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jan 2021 0 0 0
Feb 2021 7 0 0
Mar 2021 10 0 0
Apr 2021 5 0 0
May 2021 2 0 0
Jun 2021 6 0 0
Jul 2021 0 0 0