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  • 1 Department of Clinical Microbiology, Faculty of Medicine, University of Szeged Somogyi Béla tér 1, H-6725 Szeged, Hungary
  • 2 Department of Clinical Microbiology, Faculty of Medicine, University of Szeged Somogyi Béla tér 1, H-6725 Szeged, Hungary
  • 3 Department of Microbiology, Faculty of Sciences, University of Szeged P. O. Box 533, H-6701 Szeged, Hungary
  • 4 Microbiological Research Group, Hungarian Academy of Sciences and University of Szeged P.O. Box 533, H-6701 Szeged, Hungary
  • 5 Microbiological Research Group, Hungarian Academy of Sciences and University of Szeged P. O. Box 533, H-6701 Szeged, Hungary
  • 6 I: Department of Clinical Microbiology, Faculty of Medicine, University of Szeged; II: Hungarian Academy of Sciences and University of Szeged, Microbiological Research Group I: Somogyi Béla tér 1. H-6725 Szeged, Hungary; II: P. O. Box 533, H-6701 Szeged, Hungary
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The purpose of this study was to evaluate the Etest as an in vitro antifungal susceptibility test method for different moulds originating from human samples and from the environment. A total of 50 isolates (1 Acremonium, 18 Aspergillus, 2 Cladosporium, 1 Epicoccum, 15 Penicillium, 2 Scopulariopsis and 11 Trichoderma strains) were tested by the Etest. Forty-six of the tested moulds (92%) were resistant to fluconazole with minimal inhibitory concentrations (MICs) ³ 256 µg ml-1. There were strains resistant to ketoconazole among Aspergillus niger, A. ochraceus and Cladosporium spp. with MICs ? 32 µg ml-1. For fluconazole, no differences were observed using two different inocula, while for itraconazole, ketoconazole and amphotericin B, a 1 or less step 2-fold dilution difference in MIC was seen for the most of 10 selected strains. The MICs of fluconazole and amphotericin B obtained for Trichoderma strains by the Etest and the agar dilution method were also compared. MICs for fluconazole were in agreement, while MICs for amphotericin B were higher with 1 or 2 steps of 2-fold dilutions for most of Trichoderma strains in the case of the agar dilution method.

  • Pfaller, M. A., Messer, S. A., Mills, K., Bolmström, A.: In vitro susceptibility testing of filamentous fungi: Comparison of Etest and reference microdilution methods for determining itraconazole MICs. J Clin Microbiol 38, 3359-3361 (2000).

    'In vitro susceptibility testing of filamentous fungi: Comparison of Etest and reference microdilution methods for determining itraconazole MICs. ' () 38 J Clin Microbiol : 3359 -3361.

    • Search Google Scholar
  • Szekely, A., Johnson, E. M., Warnock, D. W.: Comparison of E-Test and broth microdilution methods for antifungal drug susceptibility testing of molds. J Clin Microbiol 37, 1480-1483 (1999).

    'Comparison of E-Test and broth microdilution methods for antifungal drug susceptibility testing of molds. ' () 37 J Clin Microbiol : 1480 -1483.

    • Search Google Scholar
  • Antal, Z., Manczinger, L., Szakács, G., Tengerdy, R. P., Ferenczy, L.: Colony growth, in vitro antagonism and secretion of extracellular enzymes in cold-tolerant strains of Trichoderma species. Mycol Res 104, 545-549 (2000).

    'Colony growth, in vitro antagonism and secretion of extracellular enzymes in cold-tolerant strains of Trichoderma species. ' () 104 Mycol Res : 545 -549.

    • Search Google Scholar
  • Etest technical guide No. 4: Antifungal Susceptibility Testing Of Moulds. AB Biodisk, Solna, Sweden.

    Etest technical guide No. 4: Antifungal Susceptibility Testing Of Moulds. , ().

  • Victor, L.: Antibiotics in laboratory medicine. Second Edition. Baltimore, U. S. A., 1986.

  • Campos-Herrero, M. I., Bordes, A., Perera, A., Ruiz, M. C., Fernandez, A.: Trichoderma koningii peritonitis in a patient undergoing peritoneal dialysis. Clin Microbiol Newslett 18, 150-152 (1996).

    'Trichoderma koningii peritonitis in a patient undergoing peritoneal dialysis. ' () 18 Clin Microbiol Newslett : 150 -152.

    • Search Google Scholar
  • Richter, S., Cormican, M. G., Pfaller, M. A., Lee, C. K., Gingrich, R., Rinaldi, M. G., Sutton, D. A.: Fatal disseminated Trichoderma longibrachiatum infection in an adult bone marrow transplant patient: Species identification and review of the literature. J Clin Microbiol 37, 1154-1160 (1999).

    'Fatal disseminated Trichoderma longibrachiatum infection in an adult bone marrow transplant patient: Species identification and review of the literature. ' () 37 J Clin Microbiol : 1154 -1160.

    • Search Google Scholar
  • Munoz, F. M., Demmler, G. J., Travis, W. R., Odgen, A. K., Rossmann, S. N., Rinaldi, M. G.: Trichoderma longibrachiatum infection in a pediatric patient with aplastic anemia. J Clin Microbiol 35, 499-503 (1997).

    'Trichoderma longibrachiatum infection in a pediatric patient with aplastic anemia. ' () 35 J Clin Microbiol : 499 -503.

    • Search Google Scholar
  • Singh, N.: Trends in the epidemiology of opportunistic fungal infections: predisposing factors and the impact of antimicrobial use practices. Clin Infect Dis 33, 1692-1696 (2001).

    'Trends in the epidemiology of opportunistic fungal infections: predisposing factors and the impact of antimicrobial use practices. ' () 33 Clin Infect Dis : 1692 -1696.

    • Search Google Scholar
  • Espinel-Ingroff, A.: Comparison of the E-test with the NCCLS M38-P method for antifungal susceptibility testing of common and emerging pathogenic filamentous fungi. J Clin Microbiol 39, 1360-1367 (2001).

    'Comparison of the E-test with the NCCLS M38-P method for antifungal susceptibility testing of common and emerging pathogenic filamentous fungi. ' () 39 J Clin Microbiol : 1360 -1367.

    • Search Google Scholar
  • Muñoz, P., Burillo, A., Bouza, E.: Environmental surveillance and other control measures in the prevention of nosocomial fungal infections. Clin Microbiol Infect 7 (Suppl 2), 38-45 (2001).

    'Environmental surveillance and other control measures in the prevention of nosocomial fungal infections. ' () 7 Clin Microbiol Infect : 38 -45.

    • Search Google Scholar
  • Rodríguez-Hernández, M. J., Jiménez-Mejías, M. E., Montero, J. M., Regordan, C., Ferreras, G.: Aspergillus fumigatus cranial infection after accidental traumatism. Eur J Clin Microbiol Infect Dis 20, 655-656 (2001).

    'Aspergillus fumigatus cranial infection after accidental traumatism. ' () 20 Eur J Clin Microbiol Infect Dis : 655 -656.

    • Search Google Scholar
  • Baddley, J. W., Stroud, T. P., Salzman, D., Pappas, P. G.: Invasive mold infections in allogeneic bone marrow transplant recipients. Clin Infect Dis 32, 1319-1324 (2001).

    'Invasive mold infections in allogeneic bone marrow transplant recipients. ' () 32 Clin Infect Dis : 1319 -1324.

    • Search Google Scholar
  • Yeghen, T., Fenelon, L., Campbell, C. K., Warnock, D. W., Hoffbrand, A. V., Prentice, H. G., Kibbler, C. C.: Chaetomium pneumonia in patient with acute myeloid leukaemia. J Clin Pathol 49, 184-186 (1996).

    'Chaetomium pneumonia in patient with acute myeloid leukaemia. ' () 49 J Clin Pathol : 184 -186.

    • Search Google Scholar
  • Karpovich-Tate, N., Dewey, F. M., Smith, E. J., Lund, V. J., Gurr, P. A., Gurr, S. J.: Detection of fungi in sinus fluid of patients with allergic fungal rhinosinusitis. Acta Otolaryn 120, 296-302 (2000).

    'Detection of fungi in sinus fluid of patients with allergic fungal rhinosinusitis. ' () 120 Acta Otolaryn : 296 -302.

    • Search Google Scholar
  • Revankar, S. G.: Superficial and cutaneous mycoses. Mycol Newslett November, 18 (1998).

  • Latgé, J. P.: Aspergillus fumigatus and aspergillosis. Clin Microbiol Rev 12, 310-350 (1999).

    'Aspergillus fumigatus and aspergillosis. ' () 12 Clin Microbiol Rev : 310 -350.

  • Hattori, N., Adachi, M., Kaneko, T., Shimozuma, M., Ichinohe, M., Iozumi, K.: Case report. Onychomycosis due to Chaetomium globosum successfully treated with itraconazole. Mycoses 43, 89-92 (2000).

    'Case report. Onychomycosis due to Chaetomium globosum successfully treated with itraconazole. ' () 43 Mycoses : 89 -92.

    • Search Google Scholar
  • Meletiadis, J., Meis, J. F. G. M., Mouton, J. W., Verweij, P. E.: Analysis of growth characteristics of filamentous fungi in different nutrient media. J Clin Microbiol 39, 478-484 (2001).

    'Analysis of growth characteristics of filamentous fungi in different nutrient media. ' () 39 J Clin Microbiol : 478 -484.

    • Search Google Scholar
  • National Committee for Clinical Laboratory Standards: Reference Method For Broth Dilution Antifungal Susceptibility Testing Of Yeasts. Approved standard M27-A. National Committee for Clinical Laboratory Standards, Wayne, PA. 1997.

  • National Committee for Clinical Laboratory Standards: Reference method for broth dilution antifungal susceptibility testing of conidium forming filamentous fungi. Proposed standard M38-P. National Committee for Clinical Laboratory Standards, Wayne, PA. 1998.

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