Acta Microbiologica et Immunologica Hungarica
Volume/Issue:
Volume 51: Issue 3
Production of Extracellular Proteases by Human Pathogenic Trichoderma longibrachiatum Strains
Authors: L. Kredics 1 , Zsuzsanna Antal 2 , A. Szekeres 3 , L. Manczinger 4 , Ilona Dóczi 5 , F. Kevei 6 , and Elisabeth Nagy 7
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  • 1 Microbiological Research Group, Hungarian Academy of Sciences and University of Szeged P. O. Box 533, H-6701 Szeged, Hungary
  • 2 Microbiological Research Group, Hungarian Academy of Sciences and University of Szeged P.O. Box 533, H-6701 Szeged, Hungary
  • 3 Department of Microbiology, Faculty of Sciences, University of Szeged P. O. Box 533, H-6701 Szeged, Hungary
  • 4 Department of Microbiology, Faculty of Sciences, University of Szeged P. O. Box 533, H-6701 Szeged, Hungary
  • 5 Department of Clinical Microbiology, Faculty of Medicine, University of Szeged Somogyi Béla tér 1, H-6725 Szeged, Hungary
  • 6 Department of Microbiology, Faculty of Sciences, University of Szeged P. O. Box 533, H-6701 Szeged, Hungary
  • 7 I: Microbiological Research Group, Hungarian Academy of Sciences and University of Szeged; II: Department of Clinical Microbiology, Faculty of Medicine, University of Szeged I: P. O. Box 533, H-6701 Szeged, Hungary; II: Somogyi Béla tér 1, H-6725 Szeged, Hungary
DOI:
https://doi.org/10.1556/amicr.51.2004.3.6
Page Count:
283–295
Publication Date:
01 Nov 2004
Online Publication Date:
19 Jul 2005
Article Category:
Research Article
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Species belonging to the filamentous fungal genus Trichoderma are well known as potential candidates for the biological control of plant pathogenic fungi and as cellulase producers of biotechnological importance. Several data were published in the last decade also about the clinical importance of this genus, indicating that Trichoderma strains may be potential opportunistic pathogens in immunocompromised patients. However, there is a lack of information about the potential virulence factors of clinical Trichoderma strains. This study was designed to examine the extracellular proteolytic enzymes of six clinical T. longibrachiatum isolates. Supernatants from induced liquid cultures of the examined strains were screened for proteolytic enzyme activities with 11 different chromogenic p-nitroaniline substrates. The production of trypsin-like, chymotrypsin-like and chymoelastase-like protease activities cleaving N-Benzoyl-L-Phe-L-Val-L-Arg-p-nitroanilide, N-Succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide,and N-Succinyl-L- Ala-L-Ala-L-Pro-L-Leu-p-nitroanilide, respectively, was common among the strains examined. Separation of trypsin- and chymotrypsin-like activities by column chromatography revealed, that both systems are complex consisting of several isoenzymes. The pH-dependence of these two protease systems was also studied. Based on the results, the different isoenzymes seem to have different optimal pH values. Extracellular proteolytic enzymes may be involved in the pathogenecity of Trichoderma strains as facultative human pathogens.

  • Kredics, L., Antal, Z., Manczinger, L.: Influence of water potential on growth, enzyme secretion and in vitro enzyme activities of Trichoderma harzianum at different temperatures. Curr Microbiol 40, 310-314 (2000).

    'Influence of water potential on growth, enzyme secretion and in vitro enzyme activities of Trichoderma harzianum at different temperatures. ' () 40 Curr Microbiol : 310 -314.

    • Search Google Scholar

  • Kredics, L., Dóczi, I., Antal, Z., Manczinger, L.: Effect of heavy metals on growth and extracellular enzyme activities of mycoparasitic Trichoderma strains. Bull Environ Contam Toxicol 66, 249-254 (2001).

    'Effect of heavy metals on growth and extracellular enzyme activities of mycoparasitic Trichoderma strains. ' () 66 Bull Environ Contam Toxicol : 249 -254.

    • Search Google Scholar

  • Hogan, L. H., Klein, B. S., Levitz, S. M.: Virulence factors of medically important fungi. Clin Microbiol Rev 9, 469-488 (1996).

    'Virulence factors of medically important fungi. ' () 9 Clin Microbiol Rev : 469 -488.

  • Manczinger, L., Molnár, A., Kredics, L., Antal, Z.: Production of bacteriolytic enzymes by mycoparasitic Trichoderma strains. World J Microbiol Biotechnol 18, 147-150 (2002).

    'Production of bacteriolytic enzymes by mycoparasitic Trichoderma strains. ' () 18 World J Microbiol Biotechnol : 147 -150.

    • Search Google Scholar

  • Furukawa, H., Kusne, S., Sutton, D. A., Manez, R., Carrau, R., Nichols, L., Abu-Elmagd, K., Skedros, D., Todo, S., Rinaldi, M. G.: Acute invasive sinusitis due to Trichoderma longibrachiatum in a liver and small bowel transplant recipient. Clin Infect Dis 26, 487-489 (1998).

    'Acute invasive sinusitis due to Trichoderma longibrachiatum in a liver and small bowel transplant recipient. ' () 26 Clin Infect Dis : 487 -489.

    • Search Google Scholar

  • Kubicek, C. P., Eveleigh, D. E., Esterbauer, H., Steiner, W., Kubicek-Pranz, E. M. (eds): Trichoderma reesei cellulases: biodiversity, genetics, physiology and applications. Royal Society of Chemistry, Cambridge (1990).

    Trichoderma reesei cellulases: biodiversity, genetics, physiology and applications. , ().

    • Search Google Scholar

  • Manczinger, L., Antal, Z., Kredics, L.: Ecophysiology and breeding of mycoparasitic Trichoderma strains. Acta Microbiol Immunol Hung 49, 1-14 (2002).

    'Ecophysiology and breeding of mycoparasitic Trichoderma strains. ' () 49 Acta Microbiol Immunol Hung : 1 -14.

    • Search Google Scholar

  • Kredics, L., Antal, Z., Dóczi, I., Manczinger, L., Kevei, F., Nagy, E.: Clinical importance of the genus Trichoderma. A review Acta Microbiol Immunol Hung 50, 105-117 (2003).

    'Clinical importance of the genus Trichoderma. ' () 50 A review Acta Microbiol Immunol Hung : 105 -117.

    • Search Google Scholar

  • Chouaki, T., Lavarde, V., Lachaud, L., Raccurt, C. P., Hennequin, C.: Invasive infections due to Trichoderma species: report of 2 cases, findings of in vitro susceptibility testing and review of the literature. Clin Infect Dis 35, 1360-1367 (2002).

    'Invasive infections due to Trichoderma species: report of 2 cases, findings of in vitro susceptibility testing and review of the literature. ' () 35 Clin Infect Dis : 1360 -1367.

    • Search Google Scholar

  • Myoken, Y., Sugata, T., Fujita, Y., Asaoku, H., Fujihara, M., Mikami, Y.: Fatal necrotizing stomatitis due to Trichoderma longibrachiatum in a neutropenic patient with malignant lymphoma: a case report. Int J Oral Maxillofac Surg 31, 688-691 (2002).

    'Fatal necrotizing stomatitis due to Trichoderma longibrachiatum in a neutropenic patient with malignant lymphoma: a case report. ' () 31 Int J Oral Maxillofac Surg : 688 -691.

    • Search Google Scholar

  • Esel, D., Koc, A. N., Utas, C., Karaca, N., Bozdemir, N.: Fatal peritonitis due to Trichoderma sp. in a patient undergoing continuous ambulatory peritoneal dialysis. Mycoses 46, 71-73 (2003).

    'Fatal peritonitis due to Trichoderma sp. in a patient undergoing continuous ambulatory peritoneal dialysis. ' () 46 Mycoses : 71 -73.

    • Search Google Scholar

  • Guarro, J., Antolin-Ayala, M. I., Gene, J., Gutierrez-Calzada, J., Nieves-Diez, C., Ortoneda, M.: Fatal case of Trichoderma harzianum infection in a renal transplant recipient. J Clin Microbiol 37, 3751-3755 (1999).

    'Fatal case of Trichoderma harzianum infection in a renal transplant recipient. ' () 37 J Clin Microbiol : 3751 -3755.

    • Search Google Scholar

  • Jacobs, F., Byl, B., Bourgeois, N., Coremans-Pelseneer, J., Florquin, S., Depré, G., Van de Stadt, J., Adler, M., Gelin, M., Thys, J. P.: Trichoderma viride infection in a liver transplant recipient. Mycoses 35, 301-303 (1992).

    'Trichoderma viride infection in a liver transplant recipient. ' () 35 Mycoses : 301 -303.

    • Search Google Scholar

  • Campos-Herrero, M. I., Bordes, A., Perera, A., Ruiz, M. C., Fernandez, A.: Trichoderma koningii peritonitis in a patient undergoing peritoneal dialysis. Clin Microbiol Newslett 18, 150-151 (1996).

    'Trichoderma koningii peritonitis in a patient undergoing peritoneal dialysis. ' () 18 Clin Microbiol Newslett : 150 -151.

    • Search Google Scholar

  • Gautheret, A., Dromer, F., Bourhis, J.-H., Andremont, A.: Trichoderma pseudokoningii as a cause of fatal infection in a bone marrow transplant recipient. Clin Infect Dis 20, 1063-1064 (1995).

    'Trichoderma pseudokoningii as a cause of fatal infection in a bone marrow transplant recipient. ' () 20 Clin Infect Dis : 1063 -1064.

    • Search Google Scholar

  • Kuhls, K., Lieckfeldt, E., Börner, T., Guého, E.: Molecular reidentification of human pathogenic Trichoderma isolates as Trichoderma longibrachiatum and Trichoderma citrino-viride. Med Mycol 37, 25-33 (1999).

    'Molecular reidentification of human pathogenic Trichoderma isolates as Trichoderma longibrachiatum and Trichoderma citrino-viride. ' () 37 Med Mycol : 25 -33.

    • Search Google Scholar

  • Samuels, G. J., Petrini, O., Kuhls, K., Lieckfeldt, E., Kubicek, C. P.: The Hypocrea schweinitzii complex and Trichoderma sect. longibrachiatum. Stud Mycol 41, 1-54 (1998).

    'The Hypocrea schweinitzii complex and Trichoderma sect. longibrachiatum. ' () 41 Stud Mycol : 1 -54.

    • Search Google Scholar

  • Munoz, F. M., Demmler, G. J., Travis, W. R., Ogden, A. K., Rossmann, S. N., Rinaldi, M. G.: Trichoderma longibrachiatum infection in a pediatric patient with aplastic anemia. J Clin Microbiol 35, 499-503 (1997).

    'Trichoderma longibrachiatum infection in a pediatric patient with aplastic anemia. ' () 35 J Clin Microbiol : 499 -503.

    • Search Google Scholar

  • Kredics, L., Antal, Z., Manczinger, L., Kevei, F., Nagy, E.: Proteolytic enzyme profiles of clinical Trichoderma isolates. Acta Microbiol Immunol Hung 49, 416-417 (2002).

    'Proteolytic enzyme profiles of clinical Trichoderma isolates. ' () 49 Acta Microbiol Immunol Hung : 416 -417.

    • Search Google Scholar

  • Elad, Y., Kapat, A.: The role of Trichoderma harzianum protease in the biocontrol of Botrytis cinerea. Eur J Plant Pathol 105, 177-189 (1999).

    'The role of Trichoderma harzianum protease in the biocontrol of Botrytis cinerea. ' () 105 Eur J Plant Pathol : 177 -189.

    • Search Google Scholar

  • Delgado-Jarana, J., Pintor-Toro, J. A., Benitez, T.: Overproduction of β-1, 6-glucanase in Trichoderma harzianum is controlled by extracellular acidic proteases and pH. Biochim Biophys Acta 1481, 289-296 (2000).

    'Overproduction of β-1, 6-glucanase in Trichoderma harzianum is controlled by extracellular acidic proteases and pH. ' () 1481 Biochim Biophys Acta : 289 -296.

    • Search Google Scholar

  • Geremia, R., Goldman, G. H., Jacobs, D., Ardiles, W., Vila, S. B., Van-Montagu, M., Herrera-Estrella, A.: Molecular characterization of the proteinase-encoding gene prb 1, related to mycoparasitism by Trichoderma harzianum. Mol Microbiol 8, 603-613 (1993).

    'Molecular characterization of the proteinase-encoding gene prb 1, related to mycoparasitism by Trichoderma harzianum. ' () 8 Mol Microbiol : 603 -613.

    • Search Google Scholar

  • Delgado-Jarana, J., Rincon, A. M., Benitez, T.: Aspartyl protease from Trichoderma harzianum CECT 2413: cloning and characterization. Microbiology (UK) 148, 1305-1315 (2002).

    'Aspartyl protease from Trichoderma harzianum CECT 2413: cloning and characterization. ' () 148 Microbiology (UK) : 1305 -1315.

    • Search Google Scholar

  • Suárez, B., Rey, M., Monte, E., Llobell, A.: Purification and characterization of a protease, Pral, from Trichoderma harzianum with affinity for fungal cell walls. In: Monte, E., Elad, Y. (eds): Abstracts of the 6th IOBC/WPRS-EFPP Biocontrol Workshop; Sevilla, Spain, 2000, p. 123.

  • Antal, Z., Kredics, L., Manczinger, L., Ferenczy, L.: Extracellular enzyme profiles of mycoparasitic Trichoderma strains. IOBC/wprs Bull 24(3), 337-340 (2001).

    'Extracellular enzyme profiles of mycoparasitic Trichoderma strains. ' () 24 IOBC/wprs Bull : 337 -340.

    • Search Google Scholar

  • Manczinger, L., Antal, Z., Schoop, L., Kredics, L.: Characterization of the extracellular enzyme systems of Trichoderma viride AH124. Acta Biol Hung 52, 223-229 (2001).

    'Characterization of the extracellular enzyme systems of Trichoderma viride AH124. ' () 52 Acta Biol Hung : 223 -229.

    • Search Google Scholar

  • Szekeres, A., Manczinger, L., Kredics, L., Antal, Z., Kevei, F.: Protease secretion and in vitro mycoparasitic properties of p-fluorophenylalanine resistant and morphological mutants of Trichoderma harzianum. Acta Microbiol Immunol Hung 49, 405-406 (2002).

    'Protease secretion and in vitro mycoparasitic properties of p-fluorophenylalanine resistant and morphological mutants of Trichoderma harzianum. ' () 49 Acta Microbiol Immunol Hung : 405 -406.

    • Search Google Scholar

  • Antal, Z., Manczinger, L., Szakács, G., Tengerdy, R. P., Ferenczy, L.: Colony growth, in vitro antagonism and secretion of extracellular enzymes in cold-tolerant strains of Trichoderma species. Mycol Res 104, 545-549 (2000).

    'Colony growth, in vitro antagonism and secretion of extracellular enzymes in cold-tolerant strains of Trichoderma species. ' () 104 Mycol Res : 545 -549.

    • Search Google Scholar

  • Flores, A., Chet, I., Herrera-Estrella, A.: Improved biocontrol activity of Trichoderma harzianum by over-expression of the proteinase-encoding gene prb 1. Curr Genet 31, 30-37 (1997).

    'Improved biocontrol activity of Trichoderma harzianum by over-expression of the proteinase-encoding gene prb 1. ' () 31 Curr Genet : 30 -37.

    • Search Google Scholar

  • Richter, S., Cormican, M. G., Pfaller, M. A., Lee, C. K., Gingrich, R., Rinaldi, M. G., Sutton, D. A.: Fatal disseminated Trichoderma longibrachiatum infection in an adult bone marrow transplant patient: Species identification and review of the literature. J Clin Microbiol 37, 1154-1160 (1999).

    'Fatal disseminated Trichoderma longibrachiatum infection in an adult bone marrow transplant patient: Species identification and review of the literature. ' () 37 J Clin Microbiol : 1154 -1160.

    • Search Google Scholar
Cover Acta Microbiologica et Immunologica Hungarica
Acta Microbiologica et Immunologica Hungarica A Quarterly of the Hungarian Academy of Sciences
Print ISSN:
1217-8950
Online ISSN:
1588-2640
Keywords:
Trichoderma; Trichoderma; virulence factors; opportunistic fungi; extracellular proteases; chymotrypsin; trypsin

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