Acta Microbiologica et Immunologica Hungarica
Volume/Issue:
Volume 53: Issue 1
Pulsed-Field Gel Electrophoresis: A Versatilef Tool or Analysis of Fungal Genomes A Review
Authors: Gyöngyi Lukács 1 , M. Takó 2 , and Ildikó Nyilasi 3
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  • 1 Department of Microbiology, Faculty of Sciences, University of Szeged P.O. Box 533, H-6701 Szeged, Hungary
  • 2 Department of Microbiology, Faculty of Sciences, University of Szeged P.O. Box 533, H-6701 Szeged, Hungary
  • 3 Microbiological Research Group, Hungarian Academy of Sciences and University of Szeged P.O. Box 533, H-6701 Szeged, Hungary
DOI:
https://doi.org/10.1556/amicr.53.2006.1.7
Page Count:
95–104
Publication Date:
01 Mar 2006
Online Publication Date:
06 Jun 2006
Article Category:
Research Article
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The separation of chromosome-size DNA molecules by pulsed-field gel electrophoresis (PFGE) has become a well-established technique in recent years. Although it has very wide-ranging applications, it made a real breakthrough for fungal genome analysis. Because of the small size of fungal chromosomes, their investigation was not possible earlier. Different PFGE approaches allowed the separation of DNA molecules larger than 10 megabase pairs in size, and electrophoretic karyotypes for numerous previously genetically uncharacterized fungal species could be established. This review discusses the applicability of these electrophoretic karyotypes for the investigation of genome structure, for strain identification and for species delimitation.

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Cover Acta Microbiologica et Immunologica Hungarica
Acta Microbiologica et Immunologica Hungarica A Quarterly of the Hungarian Academy of Sciences
Print ISSN:
1217-8950
Online ISSN:
1588-2640
Keywords:
chromosome-length polymorphism; electrophoretic karyotype; microscopic fungi; pulsed-field gel electrophoresis

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