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  • 1 Department of Biotechnology, Himachal Pradesh University Summer Hill, Shimla-171 005, India
  • 2 Department of Biotechnology, Himachal Pradesh University Summer Hill, Shimla-171 005, India
  • 3 Department of Biotechnology, Himachal Pradesh University Summer Hill, Shimla-171 005, India
  • 4 Department of Biotechnology, Himachal Pradesh University Summer Hill, Shimla-171 005, India
  • 5 Department of Chemistry, Himachal Pradesh University Summer Hill, Shimla-171 005, India
  • 6 Department of Biotechnology, Himachal Pradesh University Summer Hill, Shimla-171 005, India
  • 7 Department of Chemistry, Guru Nanak Dev University Amritsar-143 005, India
  • 8 Department of Chemistry, Himachal Pradesh University Summer Hill, Shimla-171 005, India
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A purified alkaline thermo-tolerant bacterial lipase from Pseudomonas aeruginosa MTCC-4713 was immobilized on a poly (AAc-co-HPMA-cl-MBAm) hydrogel. The hydrogel-bound lipase achieved 93.6% esterification of ethanol and propionic acid (300 mM: 100 mM) into ethyl propionate at temperature 65oC in 3 h in the presence of a molecular sieve (3 Å). In contrast, hydrogel-immobilized lipase pre-exposed to 5 mM of HgCl2 orNH4Cl resulted in approximately 97% conversion of reactants in 3 h into ethyl propionate under identical conditions. The salt-exposed hydrogel was relatively more efficient in repetitive esterification than the hydrogel- bound lipase not exposed to any of the cations. Moreover, bound lipase exposed Hg2+ or NH4+ ions showed altered specificity towards p-nitrophenyl esters and was more hydrolytic towards higher C-chain p-nitrophenyl esters (p-nitrophenyl laurate and p-nitrophenyl palmitate with C 12 and C 16 chain) than the immobilized lipase not exposed to any of the salts. The later showed greater specificity towards p-nitrophenyl caprylate (C 8).

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