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  • 1 Department of Biotechnology, Himachal Pradesh University Summer Hill, Shimla-171005, India
  • | 2 Department of Biotechnology, Himachal Pradesh University Summer Hill, Shimla-171005, India
  • | 3 Department of Biotechnology, Himachal Pradesh University Summer Hill, Shimla-171005, India
  • | 4 Department of Biotechnology, Himachal Pradesh University Summer Hill, Shimla-171005, India
  • | 5 Department of Biotechnology, Himachal Pradesh University Summer Hill, Shimla-171005, India
  • | 6 Department of Biotechnology, Himachal Pradesh University Summer Hill, Shimla-171005, India
  • | 7 Department of Biotechnology, Himachal Pradesh University Summer Hill, Shimla-171005, India
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A lipase from the thermophilic isolate Bacillus coagulans BTS-3 was produced and purified. The enzyme was purified 40-fold to homogeneity by ammonium sulfate precipitation and DEAE-Sepharose column chromatography. Its molecular weight was 31 kDa on SDS-PAGE. The purified lipase was immobilized on silica and its binding efficiency was found to be 60%. The enzyme took 60 min to bind maximally onto the support. The pH and temperature optima of immobilized lipase were same as those of the free enzyme, i.e. 8.5 and 55ºC, respectively.  The immobilized enzyme had shown marked thermostability on the elevated temperatures of 55, 60, 65 and 70ºC. The immobilized enzyme was reused for eigth cycles as it retained almost 80% of its activity. The catalytic activity of immobilized enzyme was enhanced in n-hexane and ethanol. The immobilized enzyme when used for esterification of ethanol and propionic acid showed 96% conversion in n-hexane in 12 h at 55ºC.

  • Jaeger, K. E., Ransac, S., Dijkstra, B. W., Colson, C., Heuvel, M., Misset, O.: Bacterial lipase. FEMS Microbiol Rev15, 29-63 (1994).

    'Bacterial lipase ' () 15 FEMS Microbiol Rev : 29 -63.

  • Verger, R.: Interfacial activation of lipase: Facts and artifacts. Trends Biotechnol15, 29-63 (1997).

    'Interfacial activation of lipase: Facts and artifacts ' () 15 Trends Biotechnol : 29 -63.

    • Search Google Scholar
  • Jaeger, K. E., Reetz, T. M.: Microbial lipases form versatile tools for biotechnology. Trends Biotechnol16, 396-403 (1998).

    'Microbial lipases form versatile tools for biotechnology ' () 16 Trends Biotechnol : 396 -403.

    • Search Google Scholar
  • Schmidt-Dannert, C.: Recombinant microbial lipases for biotechnological applications. Bioorg Med Chem7, 2123-2130 (1999).

    'Recombinant microbial lipases for biotechnological applications ' () 7 Bioorg Med Chem : 2123 -2130.

    • Search Google Scholar
  • Zaks, A., Klibanov, A. M.: Enzymatic catalysis in non-aqueous solvents. J Biol Chem263, 3194-3201 (1998).

    'Enzymatic catalysis in non-aqueous solvents ' () 263 J Biol Chem : 3194 -3201.

  • El-Rassy, H., Perrard, A., Pierre, A. C.: Behaviour of silica aerogel networks as highly porous solvent media for lipases in a model transesterification reaction. Chembiochem1, 203-210 (2003).

    'Behaviour of silica aerogel networks as highly porous solvent media for lipases in a model transesterification reaction ' () 1 Chembiochem : 203 -210.

    • Search Google Scholar
  • Kanwar, S. S., Srivastava, M., Chimni, S. S., Ghazi, I. A., Kaushal, R. K., Joshi, G. K.: Properties of an immobilized lipase of Bacillus coagulans BTS-1. Acta Microbiologica et Immunologica Hungarica51, 57-73 (2004).

    'Properties of an immobilized lipase of Bacillus coagulans BTS-1 ' () 51 Acta Microbiologica et Immunologica Hungarica : 57 -73.

    • Search Google Scholar
  • Kumar, S., Kikon, K., Upadhyay, A., Kanwar, S. S., Gupta, R.: Production, purification and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3. Protein Expression and Purification41, 38-44 (2005).

    'Production, purification and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3 ' () 41 Protein Expression and Purification : 38 -44.

    • Search Google Scholar
  • Winkler, F. K., Stuckmann, M.: Glycogen hyalouronate and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens. J Bacteriol138, 663-670 (1979).

    'Glycogen hyalouronate and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens ' () 138 J Bacteriol : 663 -670.

    • Search Google Scholar
  • Lowry, O. H., Rosenbrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement by Folin-phenol reagent. J Biol Chem193, 265-275 (1951).

    'Protein measurement by Folin-phenol reagent ' () 193 J Biol Chem : 265 -275.

  • Wisdon, R. A., Dunnill, P., Lilly, M. D., Macrae, R. A.: Enzyme esterification of fats: factors influencing the choice of support for immobilized lipase. Enzyme Microb Technol6, 443-446 (1984).

    'Enzyme esterification of fats: factors influencing the choice of support for immobilized lipase ' () 6 Enzyme Microb Technol : 443 -446.

    • Search Google Scholar
  • Shaw, J. F., Chang, R. C., Wang, K. J.: Lipolytic activities of a lipase immobilized on six selected supporting materials. Biotechnol Bioeng35, 132-137 (1990).

    'Lipolytic activities of a lipase immobilized on six selected supporting materials ' () 35 Biotechnol Bioeng : 132 -137.

    • Search Google Scholar
  • Telefoncu, A., Dinekaya, E., Vorlop, K. D.: Preparation and characterization of pancreatic lipase immobilized on Eudragit matrix. Appl Biochem Biotechnol26, 311-317 (1990).

    'Preparation and characterization of pancreatic lipase immobilized on Eudragit matrix ' () 26 Appl Biochem Biotechnol : 311 -317.

    • Search Google Scholar
  • Rizzi, M., Stylos, P., Rick, A., Reuss, M.: A kinetic study of immobilized lipase catalyzing the synthesis of isoamyl acetate by transesterification in n-hexane. Enzyme Microb Technol14, 709-713 (1992).

    'A kinetic study of immobilized lipase catalyzing the synthesis of isoamyl acetate by transesterification in n-hexane ' () 14 Enzyme Microb Technol : 709 -713.

    • Search Google Scholar
  • Balcao, V. M., Paiva, A. L., Malcata, F. X.: Bioreactors with immobilized lipases: state of the art. Enzyme Microb Technol18, 392-403 (1996).

    'Bioreactors with immobilized lipases: state of the art ' () 18 Enzyme Microb Technol : 392 -403.

    • Search Google Scholar
  • Krishnakant, S., Madamwar, D.: Ester synthesis by lipase immobilized on silica and microemulsion based organogels (MBGs). Process Biochem36, 607-611 (2001).

    'Ester synthesis by lipase immobilized on silica and microemulsion based organogels (MBGs) ' () 36 Process Biochem : 607 -611.

    • Search Google Scholar
  • Dosanjh, N. S., Kaur, J.: Immobilization, stability and esterification studies of a lipase from a Bacillus sp. Biotechnol Appl Biochem36, 7-12 (2002).

    'Immobilization, stability and esterification studies of a lipase from a Bacillus sp ' () 36 Biotechnol Appl Biochem : 7 -12.

    • Search Google Scholar
  • Roy, N., Ray, L., Chattopadhyay, P.: Production of lipase in a fermentor using a mutant strain of Corynebacterium sp.: its partial purification and immobilization. Ind J Exp Biol42, 202-207 (2004).

    'Production of lipase in a fermentor using a mutant strain of Corynebacterium sp.: its partial purification and immobilization ' () 42 Ind J Exp Biol : 202 -207.

    • Search Google Scholar
  • Palamo, J. M., Segura, R. L., Fernandez-Lorente, G., Pernas, M., Rua, M. L., Guisan, J. M., Fernandez-Lafuente, R.: Purification, immobilization and stabilization of a lipase from Bacillus thermocatenulatus by interfacial adsorption on hydrophobic supports. Biotechnol Prog20, 630-635 (2004).

    'Purification, immobilization and stabilization of a lipase from Bacillus thermocatenulatus by interfacial adsorption on hydrophobic supports ' () 20 Biotechnol Prog : 630 -635.

    • Search Google Scholar

 

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Senior editors

Editor-in-Chief: Prof. Dóra Szabó (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Managing Editor: Dr. Béla Kocsis (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Co-editor: Dr. Andrea Horváth (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Editorial Board

  • Prof. Éva ÁDÁM (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Sebastian AMYES (Department of Medical Microbiology, University of Edinburgh, Edinburgh, UK.)
  • Dr. Katalin BURIÁN (Institute of Clinical Microbiology University of Szeged, Szeged, Hungary; Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.)
  • Dr. Orsolya DOBAY (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Ildikó Rita DUNAY (Institute of Inflammation and Neurodegeneration, Medical Faculty, Otto-von-Guericke University, Magdeburg, Germany; Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany)
  • Prof. Levente EMŐDY(Department of Medical Microbiology and Immunology, University of Pécs, Pécs, Hungary.)
  • Prof. Anna ERDEI (Department of Immunology, Eötvös Loránd University, Budapest, Hungary, MTA-ELTE Immunology Research Group, Eötvös Loránd University, Budapest, Hungary.)
  • Prof. Éva Mária FENYŐ (Division of Medical Microbiology, University of Lund, Lund, Sweden)
  • Prof. László FODOR (Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary)
  • Prof. József KÓNYA (Department of Medical Microbiology, University of Debrecen, Debrecen, Hungary)
  • Prof. Yvette MÁNDI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Károly MÁRIALIGETI (Department of Microbiology, Eötvös Loránd University, Budapest, Hungary)
  • Prof. János MINÁROVITS (Department of Oral Biology and Experimental Dental Research, University of Szeged, Szeged, Hungary)
  • Prof. Béla NAGY (Centre for Agricultural Research, Institute for Veterinary Medical Research, Budapest, Hungary.)
  • Prof. István NÁSZ (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Kristóf NÉKÁM (Hospital of the Hospitaller Brothers in Buda, Budapest, Hungary.)
  • Dr. Eszter OSTORHÁZI (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Rozália PUSZTAI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Peter L. RÁDY (Department of Dermatology, University of Texas, Houston, Texas, USA)
  • Prof. Éva RAJNAVÖLGYI (Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary)
  • Prof. Ferenc ROZGONYI (Institute of Laboratory Medicine, Semmelweis University, Budapest, Hungary)
  • Prof. Zsuzsanna SCHAFF (2nd Department of Pathology, Semmelweis University, Budapest, Hungary)
  • Prof. Joseph G. SINKOVICS (The Cancer Institute, St. Joseph’s Hospital, Tampa, Florida, USA)
  • Prof. Júlia SZEKERES (Department of Medical Biology, University of Pécs, Pécs, Hungary.)
  • Prof. Mária TAKÁCS (National Reference Laboratory for Viral Zoonoses, National Public Health Center, Budapest, Hungary.)
  • Prof. Edit URBÁN (Department of Medical Microbiology and Immunology University of Pécs, Pécs, Hungary; Institute of Translational Medicine, University of Pécs, Pécs, Hungary.)

 

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2020  
Total Cites 662
WoS
Journal
Impact Factor
2,048
Rank by Immunology 145/162(Q4)
Impact Factor Microbiology 118/137 (Q4)
Impact Factor 1,904
without
Journal Self Cites
5 Year 0,671
Impact Factor
Journal  0,38
Citation Indicator  
Rank by Journal  Immunology 146/174 (Q4)
Citation Indicator  Microbiology 120/142 (Q4)
Citable 42
Items
Total 40
Articles
Total 2
Reviews
Scimago 28
H-index
Scimago 0,439
Journal Rank
Scimago Immunology and Microbiology (miscellaneous) Q4
Quartile Score Medicine (miscellaneous) Q3
Scopus 438/167=2,6
Scite Score  
Scopus General Immunology and Microbiology 31/45 (Q3)
Scite Score Rank  
Scopus 0,760
SNIP
Days from  225
sumbission
to acceptance
Days from  118
acceptance
to publication
Acceptance 19%
Rate

2019  
Total Cites
WoS
485
Impact Factor 1,086
Impact Factor
without
Journal Self Cites
0,864
5 Year
Impact Factor
1,233
Immediacy
Index
0,286
Citable
Items
42
Total
Articles
40
Total
Reviews
2
Cited
Half-Life
5,8
Citing
Half-Life
7,7
Eigenfactor
Score
0,00059
Article Influence
Score
0,246
% Articles
in
Citable Items
95,24
Normalized
Eigenfactor
0,07317
Average
IF
Percentile
7,690
Scimago
H-index
27
Scimago
Journal Rank
0,352
Scopus
Scite Score
320/161=2
Scopus
Scite Score Rank
General Immunology and Microbiology 35/45 (Q4)
Scopus
SNIP
0,492
Acceptance
Rate
16%

 

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Acta Microbiologica et Immunologica Hungarica
Language English
Size A4
Year of
Foundation
1954
Publication
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2021 Volume 68
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Issues
per Year
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Founder Magyar Tudományos Akadémia
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ISSN 1217-8950 (Print)
ISSN 1588-2640 (Online)

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