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  • 1 Regional Research Laboratory (CSIR) Division of Natural Products Chemistry Canal Road Jammu 180001 India
  • 2 Regional Research Laboratory (CSIR) Division of Pharmacology Canal Road Jammu 180001 India
  • 3 Institute of Nuclear Medicine and Allied Sciences Brig SK Mazumdar Road Delhi 110054 India
  • 4 University of Dortmund Institute for Environmental Research Campus North, Otto-Hahn-Str. 6, Chemistry Building C2-06 Dortmund Germany
  • 5 Indian Institute of Integrative Medicine Jammu Tawi India
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A novel camptothecin-producing endophytic fungus viz., Entrophospora infrequens was isolated from an important Indian medicinal plant Nothapodytes foetida . The present study reports evaluation of bioactivities of two novel extracts viz., chloroform (CEEI) and methanolic (MEEI) extracts of Entrophospora infrequens with respect to their immunomodulatory potential in vitro and in vivo (in Balb/c mice). The endophyte E. infrequens was found to synthesize camptothecin, which tested positive in CEEI. The immunomodulatory potential of CEEI and MEEI was compared with standard camptothecin (CPT). Doses of the chloroform extract (CEEI) ranging from 12.5–100 mg/kg body weight, significantly (p<0.05) stimulated the humoral and cell-mediated immune responses in a dose-dependent manner. MEEI on the other hand significantly (p<0.05) stimulated the delayed type hypersensitivity (DTH) reaction (by nearly 80%), plaque forming cell (PFC) assay (33%), phagocytic response (38%) and haemagglutination antibody (HA) titre [IgM by 79.07% and IgG by 62.05%] at a dose of 12.5 mg/kg body weight. The present study is the first report of the immunomodulatory potential of this neoteric camptothecin-producing endophyte from Nothapodytes foetida .

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