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  • 1 University of Debrecen Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology H-4010 Debrecen Hungary
  • 2 University of Debrecen Department of Biochemistry, Faculty of Science and Technology H-4010 Debrecen Hungary
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Under carbon starvation, Aspergillus nidulans produced a fungal/bacterial type chitinase, ChiB. The chiB gene was cloned and subcloned into pJC40 expression vector containing a 10XHis fusion tag, and the ChiB protein was expressed heterologously in Escherichia coli . Recombinant and native ChiB enzymes shared the same optimal pH ranges and showed similar substrate specificities with endo-acting cleavage patterns.

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