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  • 1 Debrecen University Institute of Medical Microbiology, Medical and Health Science Center Debrecen Hungary
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The performance of the VITEK 2 System (bioMérieux) version 3.01 software was compared to that of the E-test (AB Biodisk, Solna, Sweden) for antibiotic susceptibility testing of 17 clinical isolates of vancomycin resistant enterococcus (VRE). Antibiotic Susceptibility Testing (AST) by VITEK 2 produced 10 minor (59%) errors, resulting in false phenotypes. Reporting of vancomycin resistance in enterococcal strains has enormous therapeutic and epidemiological consequences. Therefore, at laboratories using automated systems (e.g. VITEK 2) for routine microbiological susceptibility testing data must be confirmed by independent validated methods, e.g. E-test, or microdilution.

  • Abele-Horn, M., Hommers, L., Trabold, R., Frosch, M.: Validation of VITEK 2 version 4.01 software for detection, identification, and classification of glycopeptide-resistant enterococci. J. Clin. Microbiol. 44, 71 (2006).

    Frosch M. , 'Validation of VITEK 2 version 4.01 software for detection, identification, and classification of glycopeptide-resistant enterococci ' (2006 ) 44 J. Clin. Microbiol. : 71 -.

    • Search Google Scholar
  • Böröcz, K., Szilágyi, E., Kurcz, A., Libbisch, B., Glatz, K., Gacs, M.: First vancomycin-resistant Enterococcus faecium outbreak reported in Hungary. Eur. Surveill. 10, E010527 (2005).

    Gacs M. , 'First vancomycin-resistant Enterococcus faecium outbreak reported in Hungary ' (2005 ) 10 Eur. Surveill. : E010527 -.

    • Search Google Scholar
  • Centers for Disease Control and Prevention. National Nosocomial Infections Surveillance (NNIS) system report. Am. J. Infect. Control 29, 404 (2001).

    'National Nosocomial Infections Surveillance (NNIS) system report ' (2001 ) 29 Am. J. Infect. Control : 404 -.

    • Search Google Scholar
  • Chang, S., Sievert, D. M., Hageman, J. C., Boulton, M. L., Tenover, F. C., Downes, F. P., Shah, S., Rudrik, J. T., Pupp, G. R., Brown, W. J., Cardo, D., Fridkin, S. K.: Infection with vancomycin-resistant Staphylococcus aureus containing the vanA resistance gene. N. England J. Med. 348, 1342 (2003).

    Fridkin S. K. , 'Infection with vancomycin-resistant Staphylococcus aureus containing the vanA resistance gene ' (2003 ) 348 N. England J. Med. : 1342 -.

    • Search Google Scholar
  • Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility testing; 19th Informational supplement M100-S15 (2009). CLSI, Wayne, PA, USA.

  • Dombrádi, Zs., Bodnár, F., Orosi, P., Dombrádi, V., Szabó, J.: A case report of vancomycin-resistant Enterococcus faecalis colonization of a femoral wound in central Europe. Central Eur. J. Med. 4, 259 (2009).

    Szabó J. , 'A case report of vancomycin-resistant Enterococcus faecalis colonization of a femoral wound in central Europe ' (2009 ) 4 Central Eur. J. Med. : 259 -.

    • Search Google Scholar
  • EARSS Management Team. EARSS annual reports. EARSS Management Team, Bilthoven, The Netherlands 2001–2006.

  • Eisner, A., Gorkiewich, G., Feierl, G., Leitner, E., Köfer, J., Kessler, H. H., Marth, E.: Identification of glycopeptide-resistant enterococci by VITEK 2 system and conventioanl and real-time polymerase chain reaction. Diagn. Microbiol. Inf. Dis. 53, 17 (2005).

    Marth E. , 'Identification of glycopeptide-resistant enterococci by VITEK 2 system and conventioanl and real-time polymerase chain reaction ' (2005 ) 53 Diagn. Microbiol. Inf. Dis. : 17 -.

    • Search Google Scholar
  • Endtz, H. P., van den Braak, N., van belkum, A., Goessens, W. H., Kreft, D., Stroebel, A. B., Verburgh, H. A.: Comparison of eight methods to detect vancomycin resistance in enterococci. J. Clin. Microbiol. 36, 592 (1998).

    Verburgh H. A. , 'Comparison of eight methods to detect vancomycin resistance in enterococci ' (1998 ) 36 J. Clin. Microbiol. : 592 -.

    • Search Google Scholar
  • Garcia-Garrote, F., Cercenado, E., Bouza, E.: Evaluation of a new system, VITEK2, for identification and antimicrobial susceptibility testing of enterococci. J. Clin. Microbiol. 38, 2108 (2000).

    Bouza E. , 'Evaluation of a new system, VITEK2, for identification and antimicrobial susceptibility testing of enterococci ' (2000 ) 38 J. Clin. Microbiol. : 2108 -.

    • Search Google Scholar
  • Ghidán, Á., Csiszár, K., Jenei, Cs., Maródi, C. L., Rozgonyi, F.: PCR detection of the vanA gene in a vancomycin resistant Enterococcus faecalis. J. Antimicrob. Chemother. 46, 325 (2000).

    Rozgonyi F. , 'PCR detection of the vanA gene in a vancomycin resistant Enterococcus faecalis ' (2000 ) 46 J. Antimicrob. Chemother. : 325 -.

    • Search Google Scholar
  • Hayden, M. K.: Insights into the epidemiology and control of infection with vancomycin-resistant enterococci. Clin. Infect. Dis. 31, 1058 (2000).

    Hayden M. K. , 'Insights into the epidemiology and control of infection with vancomycin-resistant enterococci ' (2000 ) 31 Clin. Infect. Dis. : 1058 -.

    • Search Google Scholar
  • Knausz, M., Gartner, B., Fömötör, I., Ghidán, Á., Kamotsay, K., Rozgonyi, F.: Vancomycin-resistant Enterococcus faecalis colonization during recovery from Neisseria meningitidis cerebrospinal meningitis. Acta Microbiol. Immunol. Hung. 50, 453 (2003).

    Rozgonyi F. , 'Vancomycin-resistant Enterococcus faecalis colonization during recovery from Neisseria meningitidis cerebrospinal meningitis ' (2003 ) 50 Acta Microbiol. Immunol. Hung. : 453 -.

    • Search Google Scholar
  • Kobayashi, I., Muraoka, H., Iyoda, T., Nishida, M., Hasegawa, M., Yamaguchi, K.: Antimicrobial susceptibility testing of vancomycin-resistant enterococcus by the VITEK 2 system, and comparison with two NCCLS reference methods. J. Med. Microbial. 53, 1229 (2004).

    Yamaguchi K. , 'Antimicrobial susceptibility testing of vancomycin-resistant enterococcus by the VITEK 2 system, and comparison with two NCCLS reference methods ' (2004 ) 53 J. Med. Microbial. : 1229 -.

    • Search Google Scholar
  • Kohner, P. C., Patel, R., Uhl, J. R., Garin, K. M., Hopkins, M. K., Weegenr, L. T., Cockreil, F. R.: Comparison of agar dilution, broth microdilution, E-test, disk diffusion, and automated VITEK methods fro testing susceptibilities of Enterococcus spp., to vancomycin. J. Clin. Microbiol. 35, 3258 (1997).

    Cockreil F. R. , 'Comparison of agar dilution, broth microdilution, E-test, disk diffusion, and automated VITEK methods fro testing susceptibilities of Enterococcus spp., to vancomycin ' (1997 ) 35 J. Clin. Microbiol. : 3258 -.

    • Search Google Scholar
  • Pendle, S., Jelfs, P., Olma, T., Su, Y., Gilroy, N., Gilbert, G. L.: Difficulties in detection and identification of Enterococcus faecium with low-level inducible resistance to vancomycin, during hospital outbreak. Clin. Microbiol. Infect. 14, 853 (2008).

    Gilbert G. L. , 'Difficulties in detection and identification of Enterococcus faecium with low-level inducible resistance to vancomycin, during hospital outbreak ' (2008 ) 14 Clin. Microbiol. Infect. : 853 -.

    • Search Google Scholar
  • Ramotar, K., Woods, W., Larocque, L., Toye, B.: Comparison of phenotypic methods to identify enterococci intrinsically resistant to vancomycin (vanC VRE). Diagn. Microbiol. Inf. Dis. 36, 119 (2000).

    Toye B. , 'Comparison of phenotypic methods to identify enterococci intrinsically resistant to vancomycin (vanC VRE) ' (2000 ) 36 Diagn. Microbiol. Inf. Dis. : 119 -.

    • Search Google Scholar
  • van den Braak, N., Goessens, W., van Belkum, A., Verbrugh, H. A., Endtz, H. P.: Accuracy of the VITEK 2 system to detect glycopeptide resistance in enterococci. Journal of Clinical Microbiology 39, 351 (2001).

    Endtz H. P. , 'Accuracy of the VITEK 2 system to detect glycopeptide resistance in enterococci ' (2001 ) 39 Journal of Clinical Microbiology : 351 -.

    • Search Google Scholar
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