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  • 1 Eötvös Loránd University Department of Microbiology Pázmány Péter stny. 1/C H-1117 Budapest Hungary
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The quality of drinking water is a major public concern, but the detection of most potential pathogens is not always included in drinking water hygienic monitoring or is only assessed with highly biased cultivation-based methods. In this study, the occurrence of Pseudomonas aeruginosa and Legionella spp. was examined with taxon-specific PCRs in samples taken at ten points of a municipal drinking water supply system in three months. Sequence analysis confirmed the positivity of samples and revealed a diverse community of legionellae. The results showed that chlorination was an important and effective disinfection method against pathogenic bacteria in drinking water, but pathogenic bacteria could reoccur in the system farther away from the chlorination point. No strong correlation was found between the presence of the investigated potentially pathogenic bacteria and the measured abiotic and biotic parameters within the investigated range. It is hypothesized that instead of physicochemical parameters, the main factors influencing the presence of pathogens in the drinking water were rather the composition of the microbial community, the biotic interactions between individual non-pathogenic and pathogenic microorganisms (competition or promotion of growth) and the structure of biofilm grown on the inner surface of the supply system.

  • Szewzyk, U., Szewzyk, R., Manz, W., Schleifer, K. H.: Microbiological safety of drinking water. Annu. Rev. Microbiol. 54, 81–127 (2000).

    Schleifer K. H. , 'Microbiological safety of drinking water ' (2000 ) 54 Annu. Rev. Microbiol. : 81 -127.

    • Search Google Scholar
  • Meays, C. L., Broersma, K., Nordin, R., Mazumder, A.: Source tracking of fecal bacteria in water: A critical review of current methods. J. Environ. Manage. 73, 71–79 (2004).

    Mazumder A. , 'Source tracking of fecal bacteria in water: A critical review of current methods ' (2004 ) 73 J. Environ. Manage. : 71 -79.

    • Search Google Scholar
  • Brettar, I., Höfle, M. G.: Molecular assessment of bacterial pathogens — a contribution to drinking water safety. Curr. Opin. Biotechnol. 19, 274–280 (2008).

    Höfle M. G. , 'Molecular assessment of bacterial pathogens — a contribution to drinking water safety ' (2008 ) 19 Curr. Opin. Biotechnol. : 274 -280.

    • Search Google Scholar
  • Lazcka, O., Del Campo, F. J., Muñoz, F. X.: Pathogen detection: A perspective of traditional methods and biosensors. Biosens. Bioelectron. 22, 1205–1217 (2007).

    Muñoz F. X. , 'Pathogen detection: A perspective of traditional methods and biosensors ' (2007 ) 22 Biosens. Bioelectron. : 1205 -1217.

    • Search Google Scholar
  • Diederen, B. M., de Jong, C. M., Aarts, I., Peeters, M. F., van der Zee, A.: Molecular evidence for the ubiquitous presence of Legionella species in Dutch tap water installations. J. Water Health 5, 375–383 (2007).

    Zee A. , 'Molecular evidence for the ubiquitous presence of Legionella species in Dutch tap water installations ' (2007 ) 5 J. Water Health : 375 -383.

    • Search Google Scholar
  • Lane, D. J.: 16S/23S rRNA sequencing. In: Stackebrandt, E., Goodfellow, M. (eds), Nucleic acid techniques in bacterial systematics. John Wiley and Sons, New York, 1991, pp. 115–175.

    Lane D. J. , '', in Nucleic acid techniques in bacterial systematics , (1991 ) -.

  • Polz, M. F., Cavanaugh, C. M.: Bias in template-to-product ratios in multitemplate PCR. Appl. Environ. Microbiol. 64, 3724–3730 (1998).

    Cavanaugh C. M. , 'Bias in template-to-product ratios in multitemplate PCR ' (1998 ) 64 Appl. Environ. Microbiol. : 3724 -3730.

    • Search Google Scholar
  • Jonas, D., Rosenbaum, A., Weyrich, S., Sucharit, B.: Enzyme-linked immunoassay for detection of PCR-amplified DNA of legionellae in bronchoalveolar fluid. J. Clin. Microbiol. 33, 1247–1252 (1995).

    Sucharit B. , 'Enzyme-linked immunoassay for detection of PCR-amplified DNA of legionellae in bronchoalveolar fluid ' (1995 ) 33 J. Clin. Microbiol. : 1247 -1252.

    • Search Google Scholar
  • Spilker, T., Coenye, T., Vandamme, P., LiPuma, J. J.: PCR-based assay for differentiation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cystic fibrosis patients. J. Clin. Microbiol. 42, 2074–2079 (2004).

    LiPuma J. J. , 'PCR-based assay for differentiation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cystic fibrosis patients ' (2004 ) 42 J. Clin. Microbiol. : 2074 -2079.

    • Search Google Scholar
  • Felföldi, T., Somogyi, B., Márialigeti, K., Vörös, L.: Characterization of photoautotrophic picoplankton assemblages in turbid, alkaline lakes of the Carpathian Basin (Central Europe). J. Limnol. 68, 385–395 (2009).

    Vörös L. , 'Characterization of photoautotrophic picoplankton assemblages in turbid, alkaline lakes of the Carpathian Basin (Central Europe) ' (2009 ) 68 J. Limnol. : 385 -395.

    • Search Google Scholar
  • Chun, J., Lee, J-H., Jung, Y., Kim, M., Kim, S., Kim, B. K., Lim, Y-W.: EzTaxon: A web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol. 57, 2259–2261 (2007).

    Lim Y.-W. , 'EzTaxon: A web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences ' (2007 ) 57 Int. J. Syst. Evol. Microbiol. : 2259 -2261.

    • Search Google Scholar
  • Tamura, K., Dudley, J., Nei, M., Kumar, S.: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596–1599 (2007).

    Kumar S. , 'MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 ' (2007 ) 24 Mol. Biol. Evol. : 1596 -1599.

    • Search Google Scholar
  • APHA, AWWA, WEF: Standard methods for the examination of water and wastewater, 20th ed. American Public Health Association, Washington DC, 1998, p. 1325.

    '', in Standard methods for the examination of water and wastewater , (1998 ) -.

  • Hammer, Ø., Harper, D. A. T., Ryan, P. D.: PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4, article 4 (2001).

  • Fields, B. S., Benson, R. F., Besser, R. E.: Legionella and legionnaires’s disease: 25 years of investigation. Clin. Microbiol. Rev. 15, 506–526 (2002).

    Besser R. E. , 'Legionella and legionnaires’s disease: 25 years of investigation ' (2002 ) 15 Clin. Microbiol. Rev. : 506 -526.

    • Search Google Scholar
  • Yamamoto, H., Hashimoto, Y., Ezaki, T.: Comparison of detection methods for Legionella species in environmental water by colony isolation, fluorescent antibody staining and polymerase chain reaction. Microbiol. Immunol. 37, 617–622 (1993).

    Ezaki T. , 'Comparison of detection methods for Legionella species in environmental water by colony isolation, fluorescent antibody staining and polymerase chain reaction ' (1993 ) 37 Microbiol. Immunol. : 617 -622.

    • Search Google Scholar
  • Wellinghausen, N., Frost, C., Marre, R.: Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR. Appl. Environ. Microbiol. 67, 3985–3993 (2001).

    Marre R. , 'Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR ' (2001 ) 67 Appl. Environ. Microbiol. : 3985 -3993.

    • Search Google Scholar
  • Devos, L., Clymans, K., Boon, N., Verstraete, W.: Evaluation of nested PCR assays for the detection of Legionella pneumophila in a wide range of aquatic samples. J. Appl. Microbiol. 99, 916–925 (2005).

    Verstraete W. , 'Evaluation of nested PCR assays for the detection of Legionella pneumophila in a wide range of aquatic samples ' (2005 ) 99 J. Appl. Microbiol. : 916 -925.

    • Search Google Scholar
  • Yaradou, D. F., Hallier-Soulier, S., Moreau, S. et al.: Integrated real-time PCR for the detection and monitoring of Legionella pneumophila in water systems. Appl. Environ. Microbiol. 73, 1452–1456 (2007).

    Moreau S. , 'Integrated real-time PCR for the detection and monitoring of Legionella pneumophila in water systems ' (2007 ) 73 Appl. Environ. Microbiol. : 1452 -1456.

    • Search Google Scholar
  • Felföldi, T., Heéger, Z., Vargha, M., Márialigeti, K.: Detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in Hungary. Clin. Microbiol. Infect. 16, 89–92 (2010).

    Márialigeti K. , 'Detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in Hungary ' (2010 ) 16 Clin. Microbiol. Infect. : 89 -92.

    • Search Google Scholar
  • Mena, K. D., Gerba, C. P.: Risk assessment of Pseudomonas aeruginosa in water. Rev. Environ. Contam. Toxicol. 201, 71–115 (2009).

    Gerba C. P. , 'Risk assessment of Pseudomonas aeruginosa in water ' (2009 ) 201 Rev. Environ. Contam. Toxicol. : 71 -115.

    • Search Google Scholar
  • Meenhorst, P. L., Reingold, A. L., Groothuis, D. G. et al.: Water-related nosocomial pneumonia caused by Legionella pneumophila serogroups 1 and 10. J. Infect. Dis. 152, 356–364 (1985).

    Groothuis D. G. , 'Water-related nosocomial pneumonia caused by Legionella pneumophila serogroups 1 and 10 ' (1985 ) 152 J. Infect. Dis. : 356 -364.

    • Search Google Scholar
  • Patterson, W. J., Seal, D. V., Curran, E., Sinclair, T. M., McLuckie, J. C.: Fatal nosocomial Legionnaires’ disease: Relevance of contamination of hospital water supply by temperature-dependent buoyancy-driven flow from spur pipes. Epidemiol. Infect. 112, 513–525 (1994).

    McLuckie J. C. , 'Fatal nosocomial Legionnaires’ disease: Relevance of contamination of hospital water supply by temperature-dependent buoyancy-driven flow from spur pipes ' (1994 ) 112 Epidemiol. Infect. : 513 -525.

    • Search Google Scholar
  • The European Working Group for Legionella Infections: European Guidelines for Control and Prevention of Travel Associated Legionnaires’ Disease. http://www.ewgli.org (2005)

  • Geddes, A. M., Ellis, C. J.: Infection in immunocompromised patients. QJM 55, 5–14 (1985).

    Ellis C. J. , 'Infection in immunocompromised patients ' (1985 ) 55 QJM : 5 -14.

  • Marston, B. J., Lipman, H. B., Breiman, R. F.: Surveillance for Legionnaires’ disease: Risk factors for mortality and morbidity. Arch. Intern. Med. 154, 2417–2422 (1994).

    Breiman R. F. , 'Surveillance for Legionnaires’ disease: Risk factors for mortality and morbidity ' (1994 ) 154 Arch. Intern. Med. : 2417 -2422.

    • Search Google Scholar
  • Mathys, W., Deng, M. C., Meyer, J., Junge-Mathys, E.: Fatal nosocomial Legionnaires’ disease after heart transplantation: Clinical course, epidemiology and prevention strategies for the highly immunocompromised host. J. Hosp. Infect. 43, 242–246 (1999).

    Junge-Mathys E. , 'Fatal nosocomial Legionnaires’ disease after heart transplantation: Clinical course, epidemiology and prevention strategies for the highly immunocompromised host ' (1999 ) 43 J. Hosp. Infect. : 242 -246.

    • Search Google Scholar
  • Devos, L., Boon, N., Verstraete, W.: Legionella pneumophila in the environment: the occurrence of a fastidious bacterium in oligotrophic conditions. Rev. Environ. Sci. Bio/Technol. 4, 61–74 (2005).

    Verstraete W. , 'Legionella pneumophila in the environment: the occurrence of a fastidious bacterium in oligotrophic conditions ' (2005 ) 4 Rev. Environ. Sci. Bio/Technol. : 61 -74.

    • Search Google Scholar
  • Eichler, S., Christen, R., Höltje, C., Westphal, P., Bötel, J., Brettar, I., Höfle, M. G.: Composition and dynamics of bacterial communities of a drinking water supply system as assessed by RNA/DNA-based 16S rRNA gene fingerprinting. Appl. Environ. Microbiol. 72, 1858–1872 (2006).

    Höfle M. G. , 'Composition and dynamics of bacterial communities of a drinking water supply system as assessed by RNA/DNA-based 16S rRNA gene fingerprinting ' (2006 ) 72 Appl. Environ. Microbiol. : 1858 -1872.

    • Search Google Scholar
  • Shih, H-Y., Lin, Y. E.: Caution on interpretation of Legionella results obtained using real-time PCR for environmental water samples. Appl. Environ. Microbiol. 72, 6859 (2006).

    Lin Y. E. , 'Caution on interpretation of Legionella results obtained using real-time PCR for environmental water samples ' (2006 ) 72 Appl. Environ. Microbiol. : 6859 -.

    • Search Google Scholar
  • Revetta, R. P., Pemberton, A., Lamendella, R., Iker, B., Santo Domingo, J. W.: Identification of bacterial populations in drinking water using 16S rRNA-based sequence analyses. Water Res. 44, 1353–1360 (2010).

    Santo Domingo J. W. , 'Identification of bacterial populations in drinking water using 16S rRNA-based sequence analyses ' (2010 ) 44 Water Res. : 1353 -1360.

    • Search Google Scholar
  • Cenciarini, C., Courtois, S., Raoult, D., La Scola, B.: Influence of long time storage in mineral water on RNA stability of Pseudomonas aeruginosa and Escherichia coli after heat inactivation. Public Library of Science ONE 3, e3443 (2008).

  • Kahlisch, L., Henne, K., Groebe, L., Draheim, J., Höfle, M. G., Brettar, I.: Molecular analysis of the bacterial drinking water community with respect to live/dead status. Water Sci. Technol. 61, 9–14 (2010).

    Brettar I. , 'Molecular analysis of the bacterial drinking water community with respect to live/dead status ' (2010 ) 61 Water Sci. Technol. : 9 -14.

    • Search Google Scholar
  • Kusnetsov, J. M., Martikainen, P. J., Jousimies-Somer, H. R., Väisänen, M., Tulkki, A. I., Ahonen, H. E., Nevalainen, A. I.: Physical, chemical and microbiological water characteristics associated with the occurrence of Legionella in cooling tower systems. Water Res. 27, 85–90 (1993).

    Nevalainen A. I. , 'Physical, chemical and microbiological water characteristics associated with the occurrence of Legionella in cooling tower systems ' (1993 ) 27 Water Res : 85 -90.

    • Search Google Scholar
  • Zanetti, F., Stampi, S., De Luca, G., Fateh-Moghadam, P., Antonietta, M., Sabattini, B., Checchi, L.: Water characteristics associated with the occurrence of Legionella pneumophila in dental units. Eur. J. Oral Sci. 108, 22–28 (2000).

    Checchi L. , 'Water characteristics associated with the occurrence of Legionella pneumophila in dental units ' (2000 ) 108 Eur. J. Oral Sci. : 22 -28.

    • Search Google Scholar
  • Toze, S., Sly, L. I., MacRae, I. C., Fuerst, J.: A. Inhibition of growth of Legionella species by heterotrophic plate count bacteria isolated from chlorinated drinking water. Curr. Microbiol. 21, 139–143 (1990).

    Fuerst J. , 'A. Inhibition of growth of Legionella species by heterotrophic plate count bacteria isolated from chlorinated drinking water ' (1990 ) 21 Curr. Microbiol. : 139 -143.

    • Search Google Scholar
  • Cotuk, A., Dogruoz, N., Zeybek, Z., Kimiran-Erden, A., Ilhan-Sungur, E.: The effects of Pseudomonas and Aeromonas strains on Legionella pneumophila growth. Ann. Microbiol. 55, 219–224 (2005).

    Ilhan-Sungur E. , 'The effects of Pseudomonas and Aeromonas strains on Legionella pneumophila growth ' (2005 ) 55 Ann. Microbiol. : 219 -224.

    • Search Google Scholar
  • Emtiazi, F., Schwartz, T., Marten, S. M., Krolla-Sidenstein, P., Obst, U.: Investigation of natural biofilms formed during the production of drinking water from surface water embankment filtration. Water Res. 38, 1197–1206 (2004).

    Obst U. , 'Investigation of natural biofilms formed during the production of drinking water from surface water embankment filtration ' (2004 ) 38 Water Res. : 1197 -1206.

    • Search Google Scholar
  • Grobe, S., Wingender, J., Flemming, H. C.: Capability of mucoid Pseudomonas aeruginosa to survive in chlorinated water. Int. J. Hyg. Environ. Health 204, 139–142 (2001).

    Flemming H. C. , 'Capability of mucoid Pseudomonas aeruginosa to survive in chlorinated water ' (2001 ) 204 Int. J. Hyg. Environ. Health : 139 -142.

    • Search Google Scholar
  • Berry, D., Xi, C., Raskin, L.: Microbial ecology of drinking water distribution systems. Curr. Opin. Biotechnol. 17, 297–302 (2006).

    Raskin L. , 'Microbial ecology of drinking water distribution systems ' (2006 ) 17 Curr. Opin. Biotechnol. : 297 -302.

    • Search Google Scholar
  • Cargill, K. L., Pyle, B. H., Sauer, R. L., McFeters, G. A.: Effect of culture conditions and biofilm formation on the iodine susceptibility of Legionella pneumophila. Can. J. Microbiol. 38, 423–429 (1992).

    McFeters G. A. , 'Effect of culture conditions and biofilm formation on the iodine susceptibility of Legionella pneumophila ' (1992 ) 38 Can. J. Microbiol. : 423 -429.

    • Search Google Scholar
  • Brown, M. R. W., Barker, J.: Unexplored reservoirs of pathogenic bacteria: Protozoa and biofilms. Trends Microbiol. 7, 46–50 (1999).

    Barker J. , 'Unexplored reservoirs of pathogenic bacteria: Protozoa and biofilms ' (1999 ) 7 Trends Microbiol : 46 -50.

    • Search Google Scholar
  • Homonnay, Z. G., Török, G., Brumbauer, A., Major, É., Párkány-Simon, B., Márialigeti, K., Tóth, E. M.: Bacterial communities and factors influencing their structures in drinking water network of Budapest. Acta Microbiol. Immunol. Hung. 56, Suppl. (Abstracts of the Second Central European Forum for Microbiology, October 7–9, 2009, Keszthely, Hungary) 168–169 (2009).

    Tóth E. M. , 'Bacterial communities and factors influencing their structures in drinking water network of Budapest ' (2009 ) 56 Acta Microbiol. Immunol. Hung. : 168 -169.

    • Search Google Scholar
  • Shrivastava, R., Upreti, R. K., Jain, S. R., Prasad, K. N., Seth, P. K., Chaturvedi, U. C.: Suboptimal chlorine treatment of drinking water leads to selection of multidrug-resistant Pseudomonas aeruginosa. Ecotoxicol. Environ. Saf. 58, 277–283 (2004).

    Chaturvedi U. C. , 'Suboptimal chlorine treatment of drinking water leads to selection of multidrug-resistant Pseudomonas aeruginosa ' (2004 ) 58 Ecotoxicol. Environ. Saf. : 277 -283.

    • Search Google Scholar
  • Gião, S., Wilks, S., Azevedo, N. F., Vieira, M. J., Keevil, C. W.: Incorporation of natural uncultivable Legionella pneumophila into potable water biofilms provides a protective niche against chlorination stress. Biofouling 25, 335–341 (2009).

    Keevil C. W. , 'Incorporation of natural uncultivable Legionella pneumophila into potable water biofilms provides a protective niche against chlorination stress ' (2009 ) 25 Biofouling : 335 -341.

    • Search Google Scholar
  • Leitão, J. H., Fialho, A. M., Sá-Correia, I.: Effects of growth temperature on alginate synthesis and enzymes in Pseudomonas aeruginosa variants. J. Gen. Microbiol. 138, 605–610 (1992).

    Sá-Correia I. , 'Effects of growth temperature on alginate synthesis and enzymes in Pseudomonas aeruginosa variants ' (1992 ) 138 J. Gen. Microbiol. : 605 -610.

    • Search Google Scholar
  • Moore, E. R. B., Tindall, B. J., Dos Santos, V. A. P. M., Pieper, D. H., Ramos, J-L., Palleroni, N. J.: Nonmedical: Pseudomonas. In: Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K-H., Stackebrandt, E. (eds), The Prokaryotes, Vol. 6., Proteobacteria: Gamma Subclass. Springer Science + Business Media, LLC, New York, 2006, pp. 646–703.

    Palleroni N. J. , '', in The Prokaryotes, Vol. 6 , (2006 ) -.

  • Edelstein, P. H., Cianciotto, N. P.: Legionella species and Legionnaires’ disease. In: Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K-H., Stackebrandt, E. (eds), The Prokaryotes, Vol. 6., Proteobacteria: Gamma Subclass. Springer Science + Business Media, LLC, New York, 2006, pp. 988–1033.

    Cianciotto N. P. , '', in The Prokaryotes, Vol. 6. , (2006 ) -.

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