View More View Less
  • 1 Semmelweis University Institute of Medical Microbiology Budapest Hungary
  • 2 Semmelweis University Division of Clinical Microbiology, Central Diagnostic Laboratory Budapest Hungary
  • 3 Szent István and Szent László Hospitals Central Microbiology Laboratory Budapest Hungary
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $784.00

Rapid identification of pathogens in bloodstream infections is of utmost importance to improve survival of septic patients. Fluorescent in situ hybridization (FISH) accelerates the identification of most frequent bacterial and yeast pathogens of sepsis. In this study, 210 positive blood cultures were tested with FISH method and the results were evaluated comparing to the traditional cultivation based results. Overall agreement between FISH and conventional identification was 91.4%, with better results for Gram-negative bacteria than for Gram-positives (100% and 89.5%, respectively). FISH results were obtained within 1 hour. FISH may serve as a useful tool to supplement traditional microbiological methods for rapid, provisional identification of sepsis pathogens.

  • Lever, A., MacKenzie, I.: Sepsis: Definition, epidemiology and diagnosis. BMJ 335, 879–883 (2007).

    MacKenzie I. , 'Sepsis: Definition, epidemiology and diagnosis ' (2007 ) 335 BMJ : 879 -883.

  • Stefani, S.: Diagnostic techniques in bloodstream infections: where are we going? Int. J. Antimicrob. Agents 34S, 9–12 (2009).

    Stefani S. , 'Diagnostic techniques in bloodstream infections: where are we going? ' (2009 ) 34S Int. J. Antimicrob. Agents : 9 -12.

    • Search Google Scholar
  • Moter, A., Göbel, U. B.: Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms. J. Micr. Meth. 41, 85–112 (2000).

    Göbel U. B. , 'Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms ' (2000 ) 41 J. Micr. Meth. : 85 -112.

    • Search Google Scholar
  • Peters, R. P. H., Savelkoul, P. H. M., Simoons-Smit, A. M. et al.: Rapid identification of pathogens in blood cultures with a modified FISH assay. J. Clin. Microb. 44, 4186–4188 (2006).

    Simoons-Smit A. M. , 'Rapid identification of pathogens in blood cultures with a modified FISH assay ' (2006 ) 44 J. Clin. Microb. : 4186 -4188.

    • Search Google Scholar
  • Morris, J. M., Reasonover, A. L., Bruce, M. G. et al.: Evaluation of seaFAST, a rapid fluorescent in situ hybridization test, for detection of Helicobacter pylori and resistance to clarithromycin in paraffin-embedded biopsy sections. J. Clin. Microb. 43, 3494–3496 (2005).

    Bruce M. G. , 'Evaluation of seaFAST, a rapid fluorescent in situ hybridization test, for detection of Helicobacter pylori and resistance to clarithromycin in paraffin-embedded biopsy sections ' (2005 ) 43 J. Clin. Microb. : 3494 -3496.

    • Search Google Scholar
  • Forrest, G. N.: PNA FISH: Present and future impact on patient management. Expert. Rev. Mol. Diagn. 3, 231–236 (2007).

    Forrest G. N. , 'PNA FISH: Present and future impact on patient management ' (2007 ) 3 Expert. Rev. Mol. Diagn. : 231 -236.

    • Search Google Scholar
  • Poppert, S., Essig, A., Stoehr, B. et al.: Rapid diagnosis of bacterial meningitis by real-time PCR and fluorescence in situ hybridization. J. Clin. Microbiol. 43, 3390–3397 (2005).

    Stoehr B. , 'Rapid diagnosis of bacterial meningitis by real-time PCR and fluorescence in situ hybridization ' (2005 ) 43 J. Clin. Microbiol. : 3390 -3397.

    • Search Google Scholar
  • Mallmann, C., Siemoneit, S., Schmiedel, D. et al.: Fluorescence in situ hybridization to improve the diagnosis of endocarditis: A pilot study. Clin. Microbiol. Infect. 16, 767–773 (2010).

    Schmiedel D. , 'Fluorescence in situ hybridization to improve the diagnosis of endocarditis: A pilot study ' (2010 ) 16 Clin. Microbiol. Infect. : 767 -773.

    • Search Google Scholar
  • Wellinghausen, N., Köthe, J., Wirths, B. et al.: Superiority of molecular techniques for identification of gram-negative, oxidase-positive rods, including morphologically nontypical Pseudomonas aeruginosa, from patients with cystic fibrosis. J. Clin. Microb. 43, 4070–4075 (2005).

    Wirths B. , 'Superiority of molecular techniques for identification of gram-negative, oxidase-positive rods, including morphologically nontypical Pseudomonas aeruginosa, from patients with cystic fibrosis ' (2005 ) 43 J. Clin. Microb. : 4070 -4075.

    • Search Google Scholar
  • Goddard, K. A., Townsend, R., Ridgway, E. et al.: Rapid diagnosis of intrapartum group B streptococcal carriage by fluorescent in situ hybridisation. J. Clin. Pathol. 60, 842–843 (2007).

    Ridgway E. , 'Rapid diagnosis of intrapartum group B streptococcal carriage by fluorescent in situ hybridisation ' (2007 ) 60 J. Clin. Pathol. : 842 -843.

    • Search Google Scholar
  • Wellinghausen, N., Nöckler, K., Sigge, A. et al.: Rapid detection of Brucella spp. in blood cultures by fluorescence in situ hybridization. J. Clin. Microb. 44, 1828–1830 (2006).

    Sigge A. , 'Rapid detection of Brucella spp. in blood cultures by fluorescence in situ hybridization ' (2006 ) 44 J. Clin. Microb. : 1828 -1830.

    • Search Google Scholar
  • Splettstoesser, W. D., Seibold, E., Zeman, E. et al.: Rapid differentiation of Francisella species and subspecies by fluorescent in situ hybridization targeting the 23S rRNA. BMC Microbiol. 10, 72 (2010).

    Zeman E. , 'Rapid differentiation of Francisella species and subspecies by fluorescent in situ hybridization targeting the 23S rRNA ' (2010 ) 10 BMC Microbiol. : 72 -.

    • Search Google Scholar
  • Beekmann, S. E., Diekema, D. J., Chapin, K. C. et al.: Effects of rapid detection of bloodstream infections on length of hospitalization and hospital charges. J. Clin. Microb. 41, 3119–3125 (2003).

    Chapin K. C. , 'Effects of rapid detection of bloodstream infections on length of hospitalization and hospital charges ' (2003 ) 41 J. Clin. Microb. : 3119 -3125.

    • Search Google Scholar

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jul 2020 3 0 0
Aug 2020 1 0 0
Sep 2020 2 0 0
Oct 2020 0 0 0
Nov 2020 4 3 6
Dec 2020 0 0 0
Jan 2021 2 0 0