Acta Microbiologica et Immunologica Hungarica
Volume/Issue:
Volume 57: Issue 3
Rapid identification of pathogens in blood culture with fluorescent in situ hybridization (FISH)
Authors: Andrea Horváth 1 , Katalin Kristóf, Marianne Konkoly-Thege 3 , and K. Nagy 1
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  • 1 Semmelweis University Institute of Medical Microbiology Budapest Hungary
  • 2 Semmelweis University Division of Clinical Microbiology, Central Diagnostic Laboratory Budapest Hungary
  • 3 Szent István and Szent László Hospitals Central Microbiology Laboratory Budapest Hungary
DOI:
https://doi.org/10.1556/amicr.57.2010.3.7
Page Count:
225–234
Publication Date:
01 Sep 2010
Online Publication Date:
24 Sep 2010
Article Category:
Research Article
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Rapid identification of pathogens in bloodstream infections is of utmost importance to improve survival of septic patients. Fluorescent in situ hybridization (FISH) accelerates the identification of most frequent bacterial and yeast pathogens of sepsis. In this study, 210 positive blood cultures were tested with FISH method and the results were evaluated comparing to the traditional cultivation based results. Overall agreement between FISH and conventional identification was 91.4%, with better results for Gram-negative bacteria than for Gram-positives (100% and 89.5%, respectively). FISH results were obtained within 1 hour. FISH may serve as a useful tool to supplement traditional microbiological methods for rapid, provisional identification of sepsis pathogens.

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Cover Acta Microbiologica et Immunologica Hungarica
Acta Microbiologica et Immunologica Hungarica A Quarterly of the Hungarian Academy of Sciences
Print ISSN:
1217-8950
Online ISSN:
1588-2640
Keywords:
sepsis; molecular diagnostics; FISH; in situ hybridization

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