Acta Microbiologica et Immunologica Hungarica
Volume/Issue:
Volume 58: Issue 1
Detection of Staphylococcus aureus nasal carriage in healthy young adults from a Hungarian University
Authors: Krisztina Laub 1 , Szilvia Kardos 1 , K. Nagy 1 , and Orsolya Dobay 1
View More View Less
  • 1 Semmelweis University Institute of Medical Microbiology Nagyvárad tér 4 H-1089 Budapest Hungary
DOI:
https://doi.org/10.1556/amicr.58.2011.1.8
Page Count:
75–84
Publication Date:
01 Mar 2011
Online Publication Date:
30 Mar 2011
Article Category:
Research Article
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $784.00
Purchase Online Subscription

  • Purchase article

    USD  $25.00

  • 1 year subscription (Individual Only)

    USD  $784.00

Asymptomatic carriage of Staphylococcus aureus in healthy individuals has a high prevalence, especially in children and young adults. Nasal colonisation is a well-known risk factor for subsequent severe infection, or can be the source of transmission of this bacterium to other susceptible persons. In this study, we have surveyed the nasal carriage rate of students of the Semmelweis University, by screening 300 volunteers. We have determined the antibiotic sensitivity of the isolates by Etest, and their genetic relatedness by pulsed-fieled gel electrophoresis. The nasal carriage rate of S. aureus was found to be 29.3%, and that of MRSA only 0.67% (2/300). The isolates were generally sensitive to antibiotics, except for macrolides. We could observe a noticeably great genetic diversity, even among strains deriving from students of the same university group.

  • Rehm, S. J., Tice, A.: Staphylococcus aureus: Methicillin-susceptible S. aureus to methicillin-resistant S. aureus and vancomycin-resistant S. aureus. Clin. Infect. Dis. 51, 176–182 (2010).

    Tice A. , 'Staphylococcus aureus: Methicillin-susceptible S. aureus to methicillin-resistant S. aureus and vancomycin-resistant S. aureus ' (2010 ) 51 Clin. Infect. Dis. : 176 -182.

    • Search Google Scholar

  • Sivaraman, K., Venkataraman, N., Cole, A. M.: Staphylococcus aureus nasal carriage and its contributing factors. Future Microbiol. 4, 999–1008 (2009).

    Cole A. M. , 'Staphylococcus aureus nasal carriage and its contributing factors ' (2009 ) 4 Future Microbiol. : 999 -1008.

    • Search Google Scholar

  • Cooke, F. J., Brown, N. M.: Community-associated methicillin-resistant Staphylococcus aureus infections. Br. Med. Bull. 94, 215–227 (2010).

    Brown N. M. , 'Community-associated methicillin-resistant Staphylococcus aureus infections ' (2010 ) 94 Br. Med. Bull. : 215 -227.

    • Search Google Scholar

  • Naimi, T. S., LeDell, K. H., Como-Sabetti, K., Borchardt, S. M., Boxrud, D. J., Etienne, J., Johnson, S. K., Vandenesch, F., Fridkin, S., O’Boyle, C., Danila, R. N., Lynfield, R.: Comparison of community- and health care-associated methicillin-resistant Staphylococcus aureus infection. JAMA 290, 2976–2984 (2003).

    Lynfield R. , 'Comparison of community- and health care-associated methicillin-resistant Staphylococcus aureus infection ' (2003 ) 290 JAMA : 2976 -2984.

    • Search Google Scholar

  • Kluytmans, J. A. J. W., Wertheim, H. F. L.: Nasal carriage of Staphylococcus aureus and prevention of nosocomial infections. Infect. 33, 3–8 (2005).

    Wertheim H. F. L. , 'Nasal carriage of Staphylococcus aureus and prevention of nosocomial infections ' (2005 ) 33 Infect. : 3 -8.

    • Search Google Scholar

  • Von Eiff, C., Becker, K., Machka, K., Stammer, P., Peters, G.: Nasal carriage as a source of Staphylococcus aureus bacteremia. N. Engl. J. Med. 344, 11–16 (2001).

    Peters G. , 'Nasal carriage as a source of Staphylococcus aureus bacteremia ' (2001 ) 344 N. Engl. J. Med. : 11 -16.

    • Search Google Scholar

  • Wertheim, H. F. L., Vos, M. C., Ott, A., van Belkum, A., Voss, A., Kluytmans, J. A. J. W., van Keulen, P. H. J., Vandenbroucke-Grauls, C. M. J. E., Meester, M. H. M., Verbrugh, H. A.: Risk and outcome of nosocomial Staphylococcus aureus bacteraemia in nasal carriers versus non-carriers. Lancet 364, 703–705 (2004).

    Verbrugh H. A. , 'Risk and outcome of nosocomial Staphylococcus aureus bacteraemia in nasal carriers versus non-carriers ' (2004 ) 364 Lancet : 703 -705.

    • Search Google Scholar

  • European Committee on Antimicrobial Susceptibility Testing: Breakpoint tables for interpretation of MICs and zone diameters. At http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Disk_test_documents/EUCAST_breakpoints_v1.3_pdf.pdf

  • Szabó, J., Dobay, O., Dombrádi, Z., Orosi, P., Kónya, J., Nagy, K., Rozgonyi, F.: Phenotypic and genetic characterisation of methicillin-resistant Staphylococcus aureus strains isolated from the university hospitals of Debrecen. Eur. J. Clin. Microbiol. Infect. Dis. 28, 129–136 (2009).

    Rozgonyi F. , 'Phenotypic and genetic characterisation of methicillin-resistant Staphylococcus aureus strains isolated from the university hospitals of Debrecen ' (2009 ) 28 Eur. J. Clin. Microbiol. Infect. Dis. : 129 -136.

    • Search Google Scholar

  • Struelens, M. J., Hawkey, P. M., French, G. L., Witte, W., Tacconelli, E.: Laboratory tools and strategies for methicillin-resistant Staphylococcus aureus screening, surveillance and typing: State of the art and unmet needs. Clin. Microbiol. Infect. 15, 112–119 (2009).

    Tacconelli E. , 'Laboratory tools and strategies for methicillin-resistant Staphylococcus aureus screening, surveillance and typing: State of the art and unmet needs ' (2009 ) 15 Clin. Microbiol. Infect. : 112 -119.

    • Search Google Scholar

  • Kuehnert, M. J., Kruszon-Moran, D., Hill, H. A., McQuillan, G., McAllister, F. K., Fosheim, G., McDougal, L. K., Chaitram, J., Jensen, B., Fridkin, S. K., Kilgore, G., Tenover, F. C.: Prevalence of Staphylococcus aureus nasal colonization in the United States, 2001–2002. J. Infect. Dis. 193, 172–179 (2006).

    Tenover F. C. , 'Prevalence of Staphylococcus aureus nasal colonization in the United States, 2001–2002 ' (2006 ) 193 J. Infect. Dis. : 172 -179.

    • Search Google Scholar

  • Munckhof, W. J., Nimmo, G. R., Schooneveldt, J. M., Schlebusch, S., Stephens, A. J., Williams, G., Huygens, F., Giffard, P.: Nasal carriage of Staphylococcus aureus, including community-associated methicillin-resistant strains, in Queensland adults. Clin. Microbiol. Infect. 15, 149–155 (2009).

    Giffard P. , 'Nasal carriage of Staphylococcus aureus, including community-associated methicillin-resistant strains, in Queensland adults ' (2009 ) 15 Clin. Microbiol. Infect. : 149 -155.

    • Search Google Scholar

  • Choi, C. S., Yin, C. S., Bakar, A. A., Sakewi, Z., Naing, N. N., Jamal, F., Othman, N.: Nasal carriage of Staphylococcus aureus among healthy adults. J. Microb. Immun. Infect. 39, 458–464 (2006).

    Othman N. , 'Nasal carriage of Staphylococcus aureus among healthy adults ' (2006 ) 39 J. Microb. Immun. Infect. : 458 -464.

    • Search Google Scholar

  • Mollaghan, A. M., Lucey, B., Coffey, A., Cotter, L.: Emergence of MRSA clone ST22 in healthy young adults in the community in the absence of risk factors. Epidemiol. Infect. 138, 673–676 (2010).

    Cotter L. , 'Emergence of MRSA clone ST22 in healthy young adults in the community in the absence of risk factors ' (2010 ) 138 Epidemiol. Infect. : 673 -676.

    • Search Google Scholar

  • Rohde, R. E., Denham, R., Brannon, A.: Methicillin resistant Staphylococcus aureus: carriage rates and characterization of students in a Texas university. Clin. Lab. Sci. 22, 176–184 (2009).

    Brannon A. , 'Methicillin resistant Staphylococcus aureus: carriage rates and characterization of students in a Texas university ' (2009 ) 22 Clin. Lab. Sci. : 176 -184.

    • Search Google Scholar

  • British Society for Antimicrobial Chemotherapy: Methods for Antimicrobial Susceptibility Testing. Version 9.1 March 2010. At http://www.bsac.org.uk/Resources/BSAC/Version_9.1_March_2010_final.pdf

  • Feng, Y., Chen, C.-J., Su, L.-H., Hu, S., Yu, Jun, Chiu, C.-H.: Evolution and pathogenesis of Staphylococcus aureus: lessons learned from genotyping and comparative genomics. FEMS Microbiol. Rev. 32, 23–37 (2008).

    Chiu C.-H. , 'Evolution and pathogenesis of Staphylococcus aureus: lessons learned from genotyping and comparative genomics ' (2008 ) 32 FEMS Microbiol. Rev. : 23 -37.

    • Search Google Scholar

  • Hanses, F., Huetz, T., Reischl, U., Ehrenstein, B. P., Linde, H.-J., Salzberger, B.: Lack of evidence for persistent nasal colonisation with community-acquired methicillin-resistant Staphylococcus aureus in a central European cohort. Clin. Microbiol. Infect. 17, 466–468 (2011).

    Salzberger B. , 'Lack of evidence for persistent nasal colonisation with community-acquired methicillin-resistant Staphylococcus aureus in a central European cohort ' (2011 ) 17 Clin. Microbiol. Infect. : 466 -468.

    • Search Google Scholar
Cover Acta Microbiologica et Immunologica Hungarica
Acta Microbiologica et Immunologica Hungarica A Quarterly of the Hungarian Academy of Sciences
Print ISSN:
1217-8950
Online ISSN:
1588-2640
Keywords:
nasal colonisation; carriage rate; clonality

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2020 0 1 0
Dec 2020 3 0 0
Jan 2021 0 0 0
Feb 2021 15 0 0
Mar 2021 3 0 0
Apr 2021 8 0 0
May 2021 0 0 0