Acta Microbiologica et Immunologica Hungarica
Volume/Issue:
Volume 58: Issue 3
Time-kill study and synergistic activity of cell-wall inhibitor antibiotics in combination with gentamicin against Enterococcus faecalis and Enterococcus faecium
Authors: Marzieh Aligholi 1 , Mohammad Emaneini 1 , Morovat Taherikalani 2 , Shadi Shahsavan 1 , Fereshteh Jabalameli 1 , Parisa Asadollahi 2 , Babak Khoramian 1 , and Mohammad Eslampour 3
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  • 1 Tehran University of Medical Sciences Department of Microbiology, School of Medicine Tehran Iran
  • 2 Ilam University of Medical Sciences Department of Microbiology, School of Medicine Tehran Iran
  • 3 Islamic Azad University Department of Theriogenology, Science and Research Branch Tehran Iran
DOI:
https://doi.org/10.1556/amicr.58.2011.3.5
Page Count:
219–226
Publication Date:
01 Sep 2011
Online Publication Date:
08 Oct 2011
Article Category:
Research Article
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The synergy between gentamicin and vancomycin, teicoplanin, ampicillin and linezolid was studied by time-kill method. Two clinical vancomycin resistant enterococci (VRE) and two vancomycin susceptible enterococci (VSE) isolates were used. Different concentrations of antibiotics were combined. Two VSE strains and the control strain exhibited synergism with the combination of gentamicin, vancomycin, teicoplanin, ampicillin and linezolid. Two VRE strains exhibited synergism with the combination of gentamicin and ampicillin. Synergy between gentamicin and vancomycin, teicoplanin and linezolid was not observed against these isolates. The VRE isolates were positive for vanA, aac (6′)-Ie aph (2“) and aph (3′)-IIIa genes and their vancomycin, teicoplanin and gentamicin MICs were 512 μg/ml, 512 μg/ml and >4000 μg/ml, respectively. In order to treat serious enterococcal infections, further clinical evaluation is needed to examine the in vitro combined effects of gentamicin and vancomycin, teicoplanin and linezolid.

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Cover Acta Microbiologica et Immunologica Hungarica
Acta Microbiologica et Immunologica Hungarica A Quarterly of the Hungarian Academy of Sciences
Print ISSN:
1217-8950
Online ISSN:
1588-2640
Keywords:
enterococci; VRE; VSE; antimicrobial synergy; time-kill

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