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  • 1 Himachal Pradesh University Department of Biotechnology Summer Hill Shimla 171005 India
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In the present study, the optimization of production and reaction conditions of polygalacturonase produced by a fungus Byssochlamys fulva MTCC 505 was achieved. The production of polygalacturonase with a considerable activity of 1.28 IU/ml was found when the culture was shaken at 30°C for 5 days in 100 ml of medium containing (w/v) 10 g/l pectin, 2 g/l NaNO3, 1 g/l KH2PO4, 0.5 g/l KCl, 0.5 g/l MgSO4. 7H2O, 0.001 g/l FeSO4. 7H2O, 0.001 g/l CaCl2. The best carbon and nitrogen source for this enzyme were pectin (1%) and Ca(NO3)2 (0.1%), respectively. The enzyme gave maximum activity at incubation time of 72 h, temperature of 30°C and pH 4.5. During the optimization of reaction conditions, the enzyme showed maximum activity in sodium citrate buffer (50 mM) of pH 5.5 at 50°C reaction temperature for 15 minutes of incubation. The enzyme showed greater affinity for polygalacturonic acid as substrate (0.5%). Km and Vmax values were 0.15 mg/ml and 4.58 μmol/ml/min. The effect of various phenolics, thiols, protein inhibitors and metal ions on the enzyme activity was investigated. The enzyme was quite stable at 4°C and 30°C. At 40°C the half life of the enzyme was 6 h and at 60°C it was 2 h.

  • Jayani, R. S., Saxena, S., Gupta, R.: Microbial pectinolytic enzymes: A review. Process Biochem. 40, 2931–2944 (2005).

    Gupta R. , 'Microbial pectinolytic enzymes: A review ' (2005 ) 40 Process Biochem. : 2931 -2944.

    • Search Google Scholar
  • Alkorta, I., Garbisu, C., Llama, M. J., Serra, J. L.: Industrial applications of pectic enzymes, a review. Process Biochem. 33, 21–28 (1998).

    Serra J. L. , 'Industrial applications of pectic enzymes, a review ' (1998 ) 33 Process Biochem. : 21 -28.

    • Search Google Scholar
  • Jayani, R. S., Shukla, S. K., Gupta, R.: Screening of bacterial strains for polygalacturonase activity: Its production by Bacillus sphaericus (MTCC 7542). Enzyme Res. 2010, 1–5 (2010).

    Gupta R. , 'Screening of bacterial strains for polygalacturonase activity: Its production by Bacillus sphaericus (MTCC 7542) ' (2010 ) 2010 Enzyme Res. : 1 -5.

    • Search Google Scholar
  • Sharma, N. R., Sasankan, A., Singh, A., Soni, G.: Production of polygalacturonase and pectin methyl esterase from agrowaste by using various isolates of Aspergillus niger. Insight Microbial. 1, 1–7 (2011).

    Soni G. , 'Production of polygalacturonase and pectin methyl esterase from agrowaste by using various isolates of Aspergillus niger ' (2011 ) 1 Insight Microbial. : 1 -7.

    • Search Google Scholar
  • Tochi, B. N., Wang, Z., Xu, S. Y., Zhang, W.: The influence of a pectinase and pectinase/hemicellulases enzyme preparations on percentage pineapple juice recovery particulates and sensory attributes. Pak. J. Nutr. 8, 1184–1189 (2009).

    Zhang W. , 'The influence of a pectinase and pectinase/hemicellulases enzyme preparations on percentage pineapple juice recovery particulates and sensory attributes ' (2009 ) 8 Pak. J. Nutr. : 1184 -1189.

    • Search Google Scholar
  • Murad, H. A., Azzaz, H. H.: Microbial pectinases and ruminant nutrition. Res. J. Microbiol. 6, 246–269 (2011).

    Azzaz H. H. , 'Microbial pectinases and ruminant nutrition ' (2011 ) 6 Res. J. Microbiol. : 246 -269.

    • Search Google Scholar
  • Beaven, G. H., Brown, F.: The pectic enzymes of the mould Byssochlamys fulva. Annu. Rep. Fruit Veg. Preserv. Res. Sta. Campden, 1949, p. 221.

  • Nelson, N.: A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem. 153, 375–380 (1944).

    Nelson N. , 'A photometric adaptation of the Somogyi method for the determination of glucose ' (1944 ) 153 J. Biol. Chem. : 375 -380.

    • Search Google Scholar
  • Somogyi, M.: Notes on sugar determination. J. Biol. Chem. 195, 19–25 (1952).

    Somogyi M. , 'Notes on sugar determination ' (1952 ) 195 J. Biol. Chem. : 19 -25.

  • Palaniyappan, M., Vijayagopal, V., Viswanathan, R., Viruthagiri, T.: Statistical optimization of substrate, carbon and nitrogen source by response surface methodology or pectinase production using Aspergillus fumigatus MTCC 870 in submerged fermentation. Afr. J. Biotechnol. 8, 6355–6363 (2009).

    Viruthagiri T. , 'Statistical optimization of substrate, carbon and nitrogen source by response surface methodology or pectinase production using Aspergillus fumigatus MTCC 870 in submerged fermentation ' (2009 ) 8 Afr. J. Biotechnol. : 6355 -6363.

    • Search Google Scholar
  • Makky, E. A.: Comparison of osmotic stress on growth and pectinase production by Aspergillus flavus in liquid and solid state cultures. Asian J. Exp. Sci. 23, 19–26 (2009).

    Makky E. A. , 'Comparison of osmotic stress on growth and pectinase production by Aspergillus flavus in liquid and solid state cultures ' (2009 ) 23 Asian J. Exp. Sci. : 19 -26.

    • Search Google Scholar
  • Thakur, A., Pahva, R., Singh, S., Gupta, R.: Production, purification and characterization of polygalacturonase from Mucor circinelloides ITCC 6025. Enzyme Res. 2010, 1–7 (2010).

    Gupta R. , 'Production, purification and characterization of polygalacturonase from Mucor circinelloides ITCC 6025 ' (2010 ) 2010 Enzyme Res. : 1 -7.

    • Search Google Scholar
  • Zamani, M. R., Motallebi, M., Harighi, M. J.: Pectic enzyme patterns of Fusarium oxysporum isolates from chickpea in Iran. J. Sci. I. R. Iran 12, 17–21 (2001).

    Harighi M. J. , 'Pectic enzyme patterns of Fusarium oxysporum isolates from chickpea in Iran ' (2001 ) 12 J. Sci. I. R. Iran : 17 -21.

    • Search Google Scholar
  • Mojsov, K.: Experimental investigations of submerged fermentation and synthesis of pectinolytic enzymes by Aspergillus niger: Effect of inoculum size and age of spores. Appl. Technol. Innov. 2, 40–60 (2010).

    Mojsov K. , 'Experimental investigations of submerged fermentation and synthesis of pectinolytic enzymes by Aspergillus niger: Effect of inoculum size and age of spores ' (2010 ) 2 Appl. Technol. Innov. : 40 -60.

    • Search Google Scholar
  • Rangarajan, V., Rajasekharan, M., Ravichandran, Sriganesh, R. K., Vaitheeswaran, V.: Pectinase production from orange peel extract and dried orange peel solid as substrates using Aspergillus niger. Ind. J. Biotechnol. and Biochem. 6, 445–453 (2010).

    Vaitheeswaran V. , 'Pectinase production from orange peel extract and dried orange peel solid as substrates using Aspergillus niger ' (2010 ) 6 Ind. J. Biotechnol. and Biochem. : 445 -453.

    • Search Google Scholar
  • Aguilar, G., Huitron, C.: Stimulation of the production of extracellular pectinolytic activities of Aspergillus sp. by galacturonic acid and glucose addition. Enzyme Microbiol. Technol. 9, 690–696 (1990).

    Huitron C. , 'Stimulation of the production of extracellular pectinolytic activities of Aspergillus sp. by galacturonic acid and glucose addition ' (1990 ) 9 Enzyme Microbiol. Technol. : 690 -696.

    • Search Google Scholar
  • Gupta, R., Kumar, A., Kumar, N., Bhalla, T. C.: Polygalacturonase from Aspergillus niger: Optimization of production conditions and its application. In: Marwaha, S. S., Arora, J. K. (eds): Biotechnological Strategies in Agro-processing. New Delhi: Asiatech Publishers Inc., 2003, pp. 395–400.

    Bhalla T. C. , '', in Biotechnological Strategies in Agro-processing , (2003 ) -.

  • Maldonado, M. C., Callieri, D. A. S.: Influence of environmental conditions on the production of pectinesterase and polygalacturonase by Aspergillus niger. Mircen J. 5, 327–333 (1989).

    Callieri D. A. S. , 'Influence of environmental conditions on the production of pectinesterase and polygalacturonase by Aspergillus niger ' (1989 ) 5 Mircen J. : 327 -333.

    • Search Google Scholar
  • Mathew, A., Eldo, A. N., Molly, A. G.: Optimization of culture conditions for the production of thermostable polygalacturonase by Penicillium SPC-F 20. J. Ind. Microbiol. Biotechnol. 35, 1001–1005 (2008).

    Molly A. G. , 'Optimization of culture conditions for the production of thermostable polygalacturonase by Penicillium SPC-F 20 ' (2008 ) 35 J. Ind. Microbiol. Biotechnol. : 1001 -1005.

    • Search Google Scholar
  • Kashyap, D. R., Soni, S. K., Tewari, R.: Enhanced production of pectinase by Bacillus sp. DT7 using solid state fermentation. Bioresour. Technol. 88, 251–254 (2003).

    Tewari R. , 'Enhanced production of pectinase by Bacillus sp. DT7 using solid state fermentation ' (2003 ) 88 Bioresour. Technol. : 251 -254.

    • Search Google Scholar
  • Loera, O., Aguirre, J., Viniegra-Gonzalez, G.: Pectinase production by a diploid construct from two Aspergillus niger overproducing mutants. Enz. Microbiol. Technol. 25, 103–108 (1999).

    Viniegra-Gonzalez G. , 'Pectinase production by a diploid construct from two Aspergillus niger overproducing mutants ' (1999 ) 25 Enz. Microbiol. Technol. : 103 -108.

    • Search Google Scholar
  • Gadre, R. V., Driessche, G. V., Beeumen, J. V., Bhat, M. K.: Purification, characterization and mode of action of an endo-polygalacturonase from the psychrophilic fungus Mucor flavus. Enz. Microbiol. Technol. 32, 321–330 (2003).

    Bhat M. K. , 'Purification, characterization and mode of action of an endo-polygalacturonase from the psychrophilic fungus Mucor flavus ' (2003 ) 32 Enz. Microbiol. Technol. : 321 -330.

    • Search Google Scholar
  • Freixo, M. R., Karmali, A., Arteiro, J. M.: Production, chromatographic behaviour of polygalacturonase from Pleurotus ostreatus on immobilized metal chelates. Process Biochem. 43, 531–539 (2008).

    Arteiro J. M. , 'Production, chromatographic behaviour of polygalacturonase from Pleurotus ostreatus on immobilized metal chelates ' (2008 ) 43 Process Biochem. : 531 -539.

    • Search Google Scholar
  • Beg, Q. K., Bhushan, B., Kapoor, M., Hoondal, G. S.: Effect of amino acids on production of xylanase and pectinase from Streptomyces sp QG-11-3. World J. Microbiol. Biotechnol. 16, 211–213 (2000).

    Hoondal G. S. , 'Effect of amino acids on production of xylanase and pectinase from Streptomyces sp QG-11-3 ' (2000 ) 16 World J. Microbiol. Biotechnol. : 211 -213.

    • Search Google Scholar
  • Rashad, M. M., Abdou, H. M., Shousha, W. G. H., Ali, M. M., El-Sayed, N. N.: Purification and characterization of extracellular polygalacturanase from Pleurotus oseteratus. J. Appl. Sci. Res. 6, 81–88 (2010).

    El-Sayed N. N. , 'Purification and characterization of extracellular polygalacturanase from Pleurotus oseteratus ' (2010 ) 6 J. Appl. Sci. Res. : 81 -88.

    • Search Google Scholar
  • Gillespie, A. M., Cook, K., Coughlan, M. P.: Characterization of an endopolygalacturonase produced by solid-state cultures of the aerobic fungus penicillium capsulatum. J. Biotechnol. 13, 279–292 (1990).

    Coughlan M. P. , 'Characterization of an endopolygalacturonase produced by solid-state cultures of the aerobic fungus penicillium capsulatum ' (1990 ) 13 J. Biotechnol. : 279 -292.

    • Search Google Scholar
  • Thibault, J. F., Mercier, C.: Aspergillus niger endopolygalacturonase: 2. Characterization and some properties. J. Food Biochem. 2, 379–393 (1978).

    Mercier C. , 'Aspergillus niger endopolygalacturonase: 2. Characterization and some properties ' (1978 ) 2 J. Food Biochem. : 379 -393.

    • Search Google Scholar
  • Dinu, D.: Enzymatic hydrolysis of pectic acid and pectins by polygalacturonase from Aspergillus niger. Rom. Biotechnol. Lett. 6, 397–402 (2001).

    Dinu D. , 'Enzymatic hydrolysis of pectic acid and pectins by polygalacturonase from Aspergillus niger ' (2001 ) 6 Rom. Biotechnol. Lett. : 397 -402.

    • Search Google Scholar
  • Kobayashi, T. N., Higaki, A., Suzumatsu, K., Sawada, Hagihara, S., Kawai, H., Ito, S.: Purification and properties of a high-molecular weight alkaline exopolygalacturonase from a strain of Bacillus. Enz. Microbiol. Technol. 29, 70–75 (2001).

    Ito S. , 'Purification and properties of a high-molecular weight alkaline exopolygalacturonase from a strain of Bacillus ' (2001 ) 29 Enz. Microbiol. Technol. : 70 -75.

    • Search Google Scholar
  • Mohsen, S. M., Bazaraa, W. A., Doukani, K.: Purification and characterization of Aspergillus niger U-86 polygalacturonase and its use in clarification of pomegranate and grape juices. 4th Conf. Rec. Technol. Agric. 805–817 (2009).

  • Rombouts, F. M., Pilnik, W.: Pectic enzymes. In: Rose, A. H. (ed.): Economic Microbiology, Microbial Enzymes and Bioconversions. Acad. Press. Lon. 5, 227–275 (1980).

 

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Editor-in-Chief: Prof. Dóra Szabó (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

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Co-editor: Dr. Andrea Horváth (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Editorial Board

  • Prof. Éva ÁDÁM (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Sebastian AMYES (Department of Medical Microbiology, University of Edinburgh, Edinburgh, UK.)
  • Dr. Katalin BURIÁN (Institute of Clinical Microbiology University of Szeged, Szeged, Hungary; Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.)
  • Dr. Orsolya DOBAY (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Ildikó Rita DUNAY (Institute of Inflammation and Neurodegeneration, Medical Faculty, Otto-von-Guericke University, Magdeburg, Germany; Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany)
  • Prof. Levente EMŐDY(Department of Medical Microbiology and Immunology, University of Pécs, Pécs, Hungary.)
  • Prof. Anna ERDEI (Department of Immunology, Eötvös Loránd University, Budapest, Hungary, MTA-ELTE Immunology Research Group, Eötvös Loránd University, Budapest, Hungary.)
  • Prof. Éva Mária FENYŐ (Division of Medical Microbiology, University of Lund, Lund, Sweden)
  • Prof. László FODOR (Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary)
  • Prof. József KÓNYA (Department of Medical Microbiology, University of Debrecen, Debrecen, Hungary)
  • Prof. Yvette MÁNDI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Károly MÁRIALIGETI (Department of Microbiology, Eötvös Loránd University, Budapest, Hungary)
  • Prof. János MINÁROVITS (Department of Oral Biology and Experimental Dental Research, University of Szeged, Szeged, Hungary)
  • Prof. Béla NAGY (Centre for Agricultural Research, Institute for Veterinary Medical Research, Budapest, Hungary.)
  • Prof. István NÁSZ (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Kristóf NÉKÁM (Hospital of the Hospitaller Brothers in Buda, Budapest, Hungary.)
  • Dr. Eszter OSTORHÁZI (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Rozália PUSZTAI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Peter L. RÁDY (Department of Dermatology, University of Texas, Houston, Texas, USA)
  • Prof. Éva RAJNAVÖLGYI (Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary)
  • Prof. Ferenc ROZGONYI (Institute of Laboratory Medicine, Semmelweis University, Budapest, Hungary)
  • Prof. Zsuzsanna SCHAFF (2nd Department of Pathology, Semmelweis University, Budapest, Hungary)
  • Prof. Joseph G. SINKOVICS (The Cancer Institute, St. Joseph’s Hospital, Tampa, Florida, USA)
  • Prof. Júlia SZEKERES (Department of Medical Biology, University of Pécs, Pécs, Hungary.)
  • Prof. Mária TAKÁCS (National Reference Laboratory for Viral Zoonoses, National Public Health Center, Budapest, Hungary.)
  • Prof. Edit URBÁN (Department of Medical Microbiology and Immunology University of Pécs, Pécs, Hungary; Institute of Translational Medicine, University of Pécs, Pécs, Hungary.)

 

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485
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without
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Acta Microbiologica et Immunologica Hungarica
Language English
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1954
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2021 Volume 68
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