View More View Less
  • 1 Slovak Academy of Sciences Institute of Animal Physiology Košice Slovakia
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $784.00

In children, acute otitis media (AOM) is one of the most frequently occurring infections caused by Streptococcus pneumoniae and Str. pyogenes. The standard treatment of AOMis provided by antibiotics; however, an increased resistance of the causative agents to antibiotics requires the need to search for innovations. This study was focused on in vitro testing sensitivity of streptococci isolated from AOM to enterocins produced by 9 different origin strains of E. faecium. Enterocins (Ent) represent ribosomally synthesized proteinaceous substances with antimicrobial activity against Gram-positive and/or Gram-negative bacteria which are produced mostly by strains of the species Enterococcus faecium. Str. pneumoniae were sensitive at least to 1 Ent. Str. pneumoniae SPn 754 was sensitive to 5 Ent. Five Str. pyogenes were sensitive to enterocins. Ent A (P) inhibited the growth of 3 Str. pneumoniae, and 4 Str. pyogenes (activity between 100 and 3,200 AU/ml). Most of Ent inhibited the growth of streptococci tested (100–3,200 AU/ml). Str. pyogenes were more sensitive to Ent than Str. pneumoniae. Although more detailed further studies are required, our results indicate a new possibility for enterocin use.

  • Kayser, F. H., Bienz, K. A., Eckert, J., Zinkernagel, R. M.: Medical Microbiology. Thieme, Stuttgart, New York, 2005.

    Zinkernagel R. M. , '', in Medical Microbiology , (2005 ) -.

  • Klein, J. O.: The burden of otitis media. Vaccine 19(Suppl), S2–S8 (2001).

    Klein J. O. , 'The burden of otitis media ' (2001 ) 19 Vaccine : S2 -S8.

  • Garcia-Rodrigues, J. A., Martinéz, M. J. F.: Dynamics of nasopharyngeal colonization by potential respiratory pathogens. J. Antimicrob. Chemother. 50(Suppl S2), 59–73 (2002).

    Martinéz M. J. F. , 'Dynamics of nasopharyngeal colonization by potential respiratory pathogens ' (2002 ) 50 J. Antimicrob. Chemother. : 59 -73.

    • Search Google Scholar
  • Fischetti, V. A.: Streptococcal Mprotein: Molecular design and biological behaviour. Clin. Microbiol. Rev. 2, 285–314 (1989).

    Fischetti V. A. , 'Streptococcal Mprotein: Molecular design and biological behaviour ' (1989 ) 2 Clin. Microbiol. Rev. : 285 -314.

    • Search Google Scholar
  • Kehoe, M. A.: Cell-wall associated proteins in Gram positive bacteria. N. Compr. Biochem. 27, 217–223 (1994).

    Kehoe M. A. , 'Cell-wall associated proteins in Gram positive bacteria ' (1994 ) 27 N. Compr. Biochem. : 217 -223.

    • Search Google Scholar
  • Franz, C. M. A. P., van Belkum, M. J., Holzapfel, W. H., Abriouel, H., Gálvéz, A.: Diversity of enterococcal bacteriocins and their grouping in a new classification scheme. FEMS Microbiol. Rev. 31, 293–310 (2007).

    Gálvéz A. , 'Diversity of enterococcal bacteriocins and their grouping in a new classification scheme ' (2007 ) 31 FEMS Microbiol. Rev. : 293 -310.

    • Search Google Scholar
  • Lauková, A., Czikková, S.: Inhibition effect of enterocin CCM4231 in the rumen fluid environment. Lett. Appl. Microbiol. 26, 215–218 (1998).

    Czikková S. , 'Inhibition effect of enterocin CCM4231 in the rumen fluid environment ' (1998 ) 26 Lett. Appl. Microbiol. : 215 -218.

    • Search Google Scholar
  • Lauková, A., Czikková, S., Vasilková, Z., Juriš, P., Krupicer, I.: Antimicrobial effect of enterocin CCM 4231 in the cattle slurry environment. Cytobios 94, 73–79 (1998).

    Krupicer I. , 'Antimicrobial effect of enterocin CCM 4231 in the cattle slurry environment ' (1998 ) 94 Cytobios : 73 -79.

    • Search Google Scholar
  • Lauková, A., Turek, P., Mareková, M., Nagy, J.: Use of ent M, new variant of ent P to control Listeria innocua in experimentally contaminated Gombasek sausage. Arch. Lebensmittel. 54, 46–48 (2003).

    Nagy J. , 'Use of ent M, new variant of ent P to control Listeria innocua in experimentally contaminated Gombasek sausage ' (2003 ) 54 Arch. Lebensmittel. : 46 -48.

    • Search Google Scholar
  • Lauková, A., Guba, P., Nemcová, R., Mareková, M.: Inhibition of Salmonella enterica serovar Duesseldorf in gnotobiotic Japanese quails. Vet. Med. Czech. 49, 47–51 (2004).

    Mareková M. , 'Inhibition of Salmonella enterica serovar Duesseldorf in gnotobiotic Japanese quails ' (2004 ) 49 Vet. Med. Czech. : 47 -51.

    • Search Google Scholar
  • Pogány Simonová, M., Lauková, A., Chrastinová, L’., Strompfová, V., Faix, Š., Vasilková, Z., Ondruška,L’., Jurcík, R., Rafay, J.: Enterococcus faecium CCM 7420, bacteriocin PPB CCM 7420 and their effect in the digestive tract of rabbits. Czech J. Anim. Sci. 54, 376–386 (2009).

    Rafay J. , 'Enterococcus faecium CCM 7420, bacteriocin PPB CCM 7420 and their effect in the digestive tract of rabbits ' (2009 ) 54 Czech J. Anim. Sci. : 376 -386.

    • Search Google Scholar
  • Levkut, M., Revajová, V., Lauková, A., Ševcíková, Z., Spišáková, V., Faixová, Z., Levkutová, M., Strompfová, V., Pistl, J., Levkut, M.: Leucocytic responses and intestinal mucin dynamics of broilers protected with Enterococcus faecium EF55 and challenged with Salmonella enteritidis. Res. Vet. Sci. (2011) doi:10.1016/j.rvsc.2011.06.021.

  • Aymerich, T., Holo, H., Havarstein, L. S., Hugas, M., Garriga, M., Nes, I. F.: Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins. Appl. Environ. Microbiol. 62, 1676–1682 (1996).

    Nes I. F. , 'Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins ' (1996 ) 62 Appl. Environ. Microbiol. : 1676 -1682.

    • Search Google Scholar
  • Mareková, M., Lauková, A., De Vuyst, L., Skaugen, M., Nes, I. F.: Partial characterization of bacteriocins produced by environmental strain Enterococcus faecium EK13. J. Appl. Microbiol. 94, 523–530 (2003).

    Nes I. F. , 'Partial characterization of bacteriocins produced by environmental strain Enterococcus faecium EK13 ' (2003 ) 94 J. Appl. Microbiol. : 523 -530.

    • Search Google Scholar
  • Mareková, M., Lauková, A., Skaugen, M., Nes, I. F.: Isolation and characterization of a new bacteriocin, termed enterocin M produced by environmental isolate Enterococcus faecium AL41. J. Ind. Microbiol. Biotechnol. 34, 533–537 (2007).

    Nes I. F. , 'Isolation and characterization of a new bacteriocin, termed enterocin M produced by environmental isolate Enterococcus faecium AL41 ' (2007 ) 34 J. Ind. Microbiol. Biotechnol. : 533 -537.

    • Search Google Scholar
  • De Vuyst, L., Callewaert, R., Pot, B.: Characterization of antagonistic activity of Lactobacillus amylovorus DCE471 and large-scale isolation of its bacteriocin amylovorin L471. Syst. Appl. Microbiol. 19, 9–20 (1996).

    Pot B. , 'Characterization of antagonistic activity of Lactobacillus amylovorus DCE471 and large-scale isolation of its bacteriocin amylovorin L471 ' (1996 ) 19 Syst. Appl. Microbiol. : 9 -20.

    • Search Google Scholar
  • Howell, T. H., Fiorellini, J. P. Blackburn, P., Projan, S. J., de la Harpe, J., Williams, R. C.: Effect of a mouthrinse based on nisin, a bacteriocin on developing plaque and gingivitis in beagle dogs. J. Clin. Periodont. 20, 335–339 (1993).

    Williams R. C. , 'Effect of a mouthrinse based on nisin, a bacteriocin on developing plaque and gingivitis in beagle dogs ' (1993 ) 20 J. Clin. Periodont. : 335 -339.

    • Search Google Scholar
  • Blackburn, P., Projan, S. J.: Pharmaceutical bacteriocin combinations and methods for using the same. United Patent No. 5 304 540. Appl. Microbiol. Inc., New York, USA (1994).

    Projan S. J. , '', in Pharmaceutical bacteriocin combinations and methods for using the same , (1994 ) -.

  • Hill, R. P., Farkas-Himsley, H.: Further studies of the action of a partially purified bacteriocin against a murine fibrosarcoma. Can. Res. 51, 1359–1365 (1991).

    Farkas-Himsley H. , 'Further studies of the action of a partially purified bacteriocin against a murine fibrosarcoma ' (1991 ) 51 Can. Res. : 1359 -1365.

    • Search Google Scholar
  • Kellner, R., Jung, G., Horner, T., Zähner, H., Schnell, N., Entian, K. D., Gotz, F.: Gallidermin, a new lanthionine containing polypeptide antibiotic. Eur. J. Biochem. 177, 53–59 (1988).

    Gotz F. , 'Gallidermin, a new lanthionine containing polypeptide antibiotic ' (1988 ) 177 Eur. J. Biochem. : 53 -59.

    • Search Google Scholar
  • Lauková, A., Pogány Simonová, M., Plachá, I., Strompfová, V., Cobanová, K., Szabóová, R., Chrastinová, L’.: Comparing the effect of probiotic enterococci and their enterocins in rabbits in relation to microbiological status and phagocytic activity. In Levkut et al. (eds): Intestinal Barrier of Animals and Alternative Methods of their Influence. Košice, Slovakia, 2009, pp. 22–25.

    Chrastinová L.’. , '', in Intestinal Barrier of Animals and Alternative Methods of their Influence , (2009 ) -.

  • Dluholucký, S., Frcová, B., Králinská, I., Purgel’ová, A.: Characteristics of otitis media acuta requesting hospitalization of children. Pediatry and Practise 1, 40–43 (2008).

    Purgel’ová A. , 'Characteristics of otitis media acuta requesting hospitalization of children ' (2008 ) 1 Pediatry and Practise : 40 -43.

    • Search Google Scholar
  • Pogány Simonová, M., Lauková, A., Haviarová, M.: Pseudomonads from rabbits and their sensitivity to antibiotics and natural antimicrobials. Res. Vet. Sci. 88, 203–207 (2010).

    Haviarová M. , 'Pseudomonads from rabbits and their sensitivity to antibiotics and natural antimicrobials ' (2010 ) 88 Res. Vet. Sci. : 203 -207.

    • Search Google Scholar
  • Lauková, A., Šedová, D.: Sensitivity of Pseudomonas sp. from environmental sources to antibiotics and enterocins. Program and Abstracts from 25. Congress of Czech-Slovak Society of Microbiology, 15–18. 9. 2010, Stará Lesná, p. 220. ISBN970-80-970477-8-8.

All Time Past Year Past 30 Days
Abstract Views 29 29 0
Full Text Views 5 4 0
PDF Downloads 3 0 0