Acta Microbiologica et Immunologica Hungarica
Volume/Issue:
Volume 59: Issue 3
Incidence of diarrhoeagenic Escherichia coli isolated from young children with diarrhoea in the west of Iran
Authors: Mohammad Alikhani 1 , Iraj Sedighi 2 , Alireza Zamani 3 , Mohammad Aslani 4 , and Taravat Sadrosadat 5
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  • 1 Hamadan University of Medical Sciences Department of Microbiology, Faculty of Medicine Hamadan Iran
  • 2 Hamadan University of Medical Sciences Department of Paediatrics, Faculty of Medicine Hamadan Iran
  • 3 Hamadan University of Medical Sciences Department of Immunology, Faculty of Medicine Hamadan Iran
  • 4 Pasteur Institute of Iran Department of Microbiology Tehran Iran
  • 5 Hamadan University of Medical Sciences Hamadan Iran
DOI:
https://doi.org/10.1556/amicr.59.2012.3.7
Page Count:
367–374
Publication Date:
01 Sep 2012
Online Publication Date:
14 Sep 2012
Article Category:
Research Article
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Diarrhoeagenic Escherichia coli (DEC) represents a main group of enteric pathogens that cause human diarrhoea. Because it is not simply distinguished from normal flora by simple laboratory methods, modern molecular diagnostic assays are necessary. Although it is neither necessary nor applicable to perform PCR for all patients, it is of many advantages to verify the prevalence of DEC in different areas by this method. Knowing the prevalence of DEC in an area, we can focus on few pathogens and narrow our antimicrobial treatment. The aim of this study is to evaluate the contribution of the different DEC categories in children diarrhoea in the west of Iran.The stool specimens of 251 children with diarrhoea were collected from June to September 2007. Polymerase chain reaction (PCR) was performed to determine the presence of enteropathogenic (EPEC), enterotoxigenic (ETEC), entero-invasive (EIEC), Shiga toxin-producing (STEC) and entero-aggregative (EAEC) strains. ETEC strains were isolated from 13 and EAEC strains from 16 children. STEC was detected in 7 children, and no EIEC was isolated. Finally, EPEC strains were isolated in 41 cases. EAEC and EPEC are the most frequent DECs in children less than 10 years of age in West of Iran.

  • Bern, C., Martines, J., de Zoysa, I., Glass, R. I.: The magnitude of the global problem of diarrhoeal disease: A ten-year update. Bull World Health Organ 70, 705–714 (1992).

    Glass R. I. , 'The magnitude of the global problem of diarrhoeal disease: A ten-year update ' (1992 ) 70 Bull World Health Organ : 705 -714.

    • Search Google Scholar

  • Bhutta, Z. A.: Acute gastroenteritis in children. In Kliegman, R. M. (ed.): Nelson Text Book of Pediatrics. 19th Edition. Elsevier, Philadelphia, 2011, pp. 1323–1338.

    Bhutta Z. A. , '', in Nelson Text Book of Pediatrics , (2011 ) -.

  • Parashar, U., Umesh, D., Bresee, J. S., Joseph, S., Glass, R. I., Roger, I.: The global burden of diarrhoeal disease in children. Bull World Health Organ 81, 236 (2003).

    Roger I. , 'The global burden of diarrhoeal disease in children ' (2003 ) 81 Bull World Health Organ : 236 -.

    • Search Google Scholar

  • Snyder, J.tD., Merson, M. H.: The magnitude of the global problem of acute diarrhoeal disease: A review of active surveillance data. Bull World Health Organ 60, 605–613 (1982).

    Merson M. H. , 'The magnitude of the global problem of acute diarrhoeal disease: A review of active surveillance data ' (1982 ) 60 Bull World Health Organ : 605 -613.

    • Search Google Scholar

  • Kosek, M., Bern, C., Guerrant, R. L.: The global burden of diarrhoeal disease, as estimated from studies published between 1992 and 2000. Bull World Health Organ 81, 197–204 (2003).

    Guerrant R. L. , 'The global burden of diarrhoeal disease, as estimated from studies published between 1992 and 2000 ' (2003 ) 81 Bull World Health Organ : 197 -204.

    • Search Google Scholar

  • Vargas, M., Gascon, J., Casals, C., Schellenberg, D., Urassa, H., Kahigwa, E.: Etiology of diarrhea in children less than five years of age in Ifakara, Tanzania. Am J Trop Med Hyg 70, 536–539 (2004).

    Kahigwa E. , 'Etiology of diarrhea in children less than five years of age in Ifakara, Tanzania ' (2004 ) 70 Am J Trop Med Hyg : 536 -539.

    • Search Google Scholar

  • Guerrant, R. L., Hughes, J. M., Lima, N. L., Crane, J.: Diarrhoea in developed and developing countries: Magnitude, special settings, and etiologies. Rev Infect Dis 12, 41–50 (1990).

    Crane J. , 'Diarrhoea in developed and developing countries: Magnitude, special settings, and etiologies ' (1990 ) 12 Rev Infect Dis : 41 -50.

    • Search Google Scholar

  • Nataro, J. P., Kaper, J. B.: Diarrheagenic Escherichia coli. Clin Microbiol Rev 11, 142–201 (1998).

    Kaper J. B. , 'Diarrheagenic Escherichia coli ' (1998 ) 11 Clin Microbiol Rev : 142 -201.

  • Rappelli, P., Maddau, G., Mannu, F., Colombo, M. M., Fiori, P. L., Cappuccinelli, P.: Development of a set of multiplex PCR assays for the simultaneous identification of enterotoxigenic, enteropathogenic, enterohemorrhagic and enteroinvasive Escherichia coli. New Microbiol 24, 77–83 (2001).

    Cappuccinelli P. , 'Development of a set of multiplex PCR assays for the simultaneous identification of enterotoxigenic, enteropathogenic, enterohemorrhagic and enteroinvasive Escherichia coli ' (2001 ) 24 New Microbiol : 77 -83.

    • Search Google Scholar

  • Stacy-Phipps, S., Mecca, J. J., Weiss, J. B.: Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenic Escherichia coli DNA during course of infection. J Clin Microbiol 33, 1054–1059 (1995).

    Weiss J. B. , 'Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenic Escherichia coli DNA during course of infection ' (1995 ) 33 J Clin Microbiol : 1054 -1059.

    • Search Google Scholar

  • Beaudry, M., Zhu, C., Fairbrother, J. M., Harel, J.: Genotypic and phenotypic characterization of Escherichia coli isolates from dogs manifesting attaching and effacing lesions. J Clin Microbiol 34, 144–148 (1996).

    Harel J. , 'Genotypic and phenotypic characterization of Escherichia coli isolates from dogs manifesting attaching and effacing lesions ' (1996 ) 34 J Clin Microbiol : 144 -148.

    • Search Google Scholar

  • Blomen, I., Lofdahl, S., Stenstrom, T. A., Norberg, R.: Identification of enterotoxigenic Escherichia coli isolates, a comparison of PCR, DNA hybridisation, ELISAs and bioassays. J Microbiol Methods 17, 181–191 (1993).

    Norberg R. , 'Identification of enterotoxigenic Escherichia coli isolates, a comparison of PCR, DNA hybridisation, ELISAs and bioassays ' (1993 ) 17 J Microbiol Methods : 181 -191.

    • Search Google Scholar

  • Frankel, G., Riley, L., Giron, J. A., Valmassoi, J., Friedmann, A., Strockbine, N.: Detection of Shigella in feces using DNA amplification. J Infect Dis 161, 1252–1256 (1990).

    Strockbine N. , 'Detection of Shigella in feces using DNA amplification ' (1990 ) 161 J Infect Dis : 1252 -1256.

    • Search Google Scholar

  • Lin, Z., Kurazono, H., Yamasaki, S., Takeda, Y.: Detection of various variant Verotoxin genes in Escherichia coli by polymerase chain reaction. Microbiol Immunol 37, 543–548 (1993).

    Takeda Y. , 'Detection of various variant Verotoxin genes in Escherichia coli by polymerase chain reaction ' (1993 ) 37 Microbiol Immunol : 543 -548.

    • Search Google Scholar

  • Schmidt, H., Knop, C., Franke, S., Aleksic, S., Heesemann, J., Karch, H.: Development of PCR for screening of enteroaggregative Escherichia coli. J Clin Microbiol 33, 70–75 (1995).

    Karch H. , 'Development of PCR for screening of enteroaggregative Escherichia coli ' (1995 ) 33 J Clin Microbiol : 70 -75.

    • Search Google Scholar

  • Ochoa, T. J., Cleary, T. G.: Escherichia coli. In Kliegman, R. M. (ed.): Nelson Text Book of Pediatrics. Elsevier, Philadelphia, 2011, pp. 961–965.

    Cleary T. G. , '', in Nelson Text Book of Pediatrics , (2011 ) -.

  • Okeke, I. N., Lamikanra, A., Czeczulin, J., Dubovsky, F., Kaper, J., Nataro, J.: Heterogeneous virulence of enteroaggregative Escherichia coli strains isolated from children in Southwest Nigeria. J Infect Dis 181, 252–260 (2000).

    Nataro J. , 'Heterogeneous virulence of enteroaggregative Escherichia coli strains isolated from children in Southwest Nigeria ' (2000 ) 181 J Infect Dis : 252 -260.

    • Search Google Scholar

  • Presterl, E., Zwick, R. H., Reichmann, S., Ichelburg, A., Winkler, S., Kremsner, P. G.: Frequency and virulence properties of diarrheagenic Escherichia coli in children with diarrhea in Gabon. Am J Trop Med Hyg 69, 406–410 (2003).

    Kremsner P. G. , 'Frequency and virulence properties of diarrheagenic Escherichia coli in children with diarrhea in Gabon ' (2003 ) 69 Am J Trop Med Hyg : 406 -410.

    • Search Google Scholar

  • Sarantuya, J., Nishi, J., Wakimoto, N., Erdene, S., Nataro, J., Sheikh, J.: Typical enteroaggregative Escherichia coli is the most prevalent pathotype among E. coli strains causing diarrhea in Mongolian children. J Clin Microbiol 42, 133–139 (2004).

    Sheikh J. , 'Typical enteroaggregative Escherichia coli is the most prevalent pathotype among E. coli strains causing diarrhea in Mongolian children ' (2004 ) 42 J Clin Microbiol : 133 -139.

    • Search Google Scholar

  • Savarino, S. J.: Diarrhoeal disease: Current concepts and future challenges. Enteroadherent Escherichia coli: A heterogeneous group of E. coli implicated as diarrhoeal pathogens. Trans R Soc Trop Med Hyg 87, 49–53 (1993).

    Savarino S. J. , 'Diarrhoeal disease: Current concepts and future challenges. Enteroadherent Escherichia coli: A heterogeneous group of E. coli implicated as diarrhoeal pathogens ' (1993 ) 87 Trans R Soc Trop Med Hyg : 49 -53.

    • Search Google Scholar

  • Gonzalez, R., Diaz, C., Marino, M., Cloralt, R., Pequeneze, M., Perez-Schael, I.: Age-specific prevalence of Escherichia coli with localized and aggregative adherence in venezuela infants with acute diarrhea. J Clin Microbiol 35, 1103–1107 (1997).

    Perez-Schael I. , 'Age-specific prevalence of Escherichia coli with localized and aggregative adherence in venezuela infants with acute diarrhea ' (1997 ) 35 J Clin Microbiol : 1103 -1107.

    • Search Google Scholar

  • Afset, J., Bergh, K., Bevanger, L.: High prevalence of atypical enteropathogenic Escherichia coli (EPEC) in Norwegian children with diarrhoea. J Med Microbiol 52, 1015–1019 (2003).

    Bevanger L. , 'High prevalence of atypical enteropathogenic Escherichia coli (EPEC) in Norwegian children with diarrhoea ' (2003 ) 52 J Med Microbiol : 1015 -1019.

    • Search Google Scholar

  • Afset, J., Bevanger, L., Romundstad, P., Bergh, K.: Association of atypical enteropathogenic Escherichia coli (EPEC) with prolonged diarrhoea. J Med Microbiol 53, 1137–1144 (2004).

    Bergh K. , 'Association of atypical enteropathogenic Escherichia coli (EPEC) with prolonged diarrhoea ' (2004 ) 53 J Med Microbiol : 1137 -1144.

    • Search Google Scholar

  • Cegielski, J., Msengi, A., Dukes, C. S., Levine, M. M.: Pathogenic Escherichia coli in children with and without chronic diarrhea in Tanzania. J Infect Dis 174, 675–877 (1996).

    Levine M. M. , 'Pathogenic Escherichia coli in children with and without chronic diarrhea in Tanzania ' (1996 ) 174 J Infect Dis : 675 -877.

    • Search Google Scholar

  • Elias, W. P., Uber, A. P., Tomita, S. K., Trabulsi, L. R., Gomes, T. A.: Combinations of putative virulence markers in typical and variant enteroaggregative Escherichia coli strains from children with and without diarrhoea. Epidemiol Infect 129, 49–55 (2002).

    Gomes T. A. , 'Combinations of putative virulence markers in typical and variant enteroaggregative Escherichia coli strains from children with and without diarrhoea ' (2002 ) 129 Epidemiol Infect : 49 -55.

    • Search Google Scholar

  • Gunzburg, S. T., Chang, B. J., Burke, V., Gracey, M.: Virulence factors of enteric Escherichia coli in young Aboriginal children in northwest Australia. Epidemiol Infect 109, 283–289 (1992).

    Gracey M. , 'Virulence factors of enteric Escherichia coli in young Aboriginal children in northwest Australia ' (1992 ) 109 Epidemiol Infect : 283 -289.

    • Search Google Scholar

  • Okeke, I. N., Lamikanra, A., Steinruck, H., Kaper, J. B.: Characterization of Escherichia coli strains from cases of childhood diarrhea in provincial southwestern Nigeria. J Clin Microbiol 38, 7–12 (2000).

    Kaper J. B. , 'Characterization of Escherichia coli strains from cases of childhood diarrhea in provincial southwestern Nigeria ' (2000 ) 38 J Clin Microbiol : 7 -12.

    • Search Google Scholar
Cover Acta Microbiologica et Immunologica Hungarica
Acta Microbiologica et Immunologica Hungarica A Quarterly of the Hungarian Academy of Sciences
Print ISSN:
1217-8950
Online ISSN:
1588-2640
Keywords:
diarrhoeagenic E. coli; diarrhoea; polymerase chain reaction; Iran

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