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  • 1 University of Debrecen Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology Debrecen Hungary
  • 2 University of Debrecen Department of Inorganic and Analytical Chemistry, Faculty of Science and Technology Debrecen Hungary
  • 3 Eszterházy Károly College Department of Zoology, Faculty of Sciences Eger Hungary
  • 4 University of Debrecen Department of Nephrology, Medical and Health Science Center Debrecen Hungary
  • 5 Hungarian Academy of Sciences Vascular Biology, Thrombosis and Hemostasis Research Group Debrecen Hungary
  • 6 University of Debrecen Department of Neonatology, Medical and Health Science Center Debrecen Hungary
  • 7 University of Debrecen Department of Physiology, Medical and Health Science Center Debrecen Hungary
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Iron is an essential element for all microorganisms. Bacteria and fungi produce versatile siderophores for binding and storing this essential transition metal when its availability is limited in the environment. The aim of the study was to optimize the fermentation medium of Aspergillus fumigatus for siderophore production. Triacetyl-fusarinine C and ferricrocin yields were dependent on glucose and glycine supplementations as well as the initial pH of the culture media. The optimal fermentation medium for triacetylfusarinine C production contained 8% glucose, 0.4% glycine and the initial pH was set to 5.9. Meanwhile, maximal ferricrocin yields were recorded in the presence of 10% glucose, 0.5% glycine and at an initial pH of 7.4. Under optimized fermentation conditions, the yields for triacetylfusarinine C and ferricrocin increased up to 2.9 g/l culture medium and 18.9 mg/g mycelium, respectively.

  • Hider, R.C., Kong, X.: Chemistry and biology of siderophores. Nat Prod Rep 27, 637–657 (2010).

    Kong X. , 'Chemistry and biology of siderophores ' (2010 ) 27 Nat Prod Rep : 637 -657.

  • Johnson, L.: Iron and siderophores in fungal-host interactions. Mycol Res 112, 170–183 (2008).

    Johnson L. , 'Iron and siderophores in fungal-host interactions ' (2008 ) 112 Mycol Res : 170 -183.

    • Search Google Scholar
  • Oide, S., Krasnoff, S.B., Gibson, D.M., Turgeon, B.G.: Intracellular siderophores are essential for ascomycete sexual development in heterothallic Cochliobolus heterostrophus and homothallic Gibberella zeae. Eukaryot Cell 6, 1339–1353 (2007).

    Turgeon B.G. , 'Intracellular siderophores are essential for ascomycete sexual development in heterothallic Cochliobolus heterostrophus and homothallic Gibberella zeae ' (2007 ) 6 Eukaryot Cell : 1339 -1353.

    • Search Google Scholar
  • Haas, H.: Iron — a key nexus in the virulence of Aspergillus fumigatus. Frontiers in Microbiol 3, 1–10 (2012).

    Haas H. , 'Iron — a key nexus in the virulence of Aspergillus fumigatus ' (2012 ) 3 Frontiers in Microbiol : 1 -10.

    • Search Google Scholar
  • Schrettl, M., Beckmann, N., Varga, J., Heinekamp, T., Jacobsen, I.D., Jöchl, C., Moussa, T.A., Wang, S., Gsaller, F., Blatzer, M., Werner, E.R., Niermann, W.C., Brakhage, A.A., Haas, H.: HapX-mediated adaption to iron starvation is crucial for virulence of Aspergillus fumigatus. PLoS Pathog 6, e1001124 (2010).

    Haas H. , 'HapX-mediated adaption to iron starvation is crucial for virulence of Aspergillus fumigatus ' (2010 ) 6 PLoS Pathog : e1001124 -.

    • Search Google Scholar
  • Haas, H.: Molecular genetics of fungal siderophore biosynthesis and uptake: The role of siderophores in iron uptake and storage. Appl Microbiol Biotechnol 62, 316–330 (2003).

    Haas H. , 'Molecular genetics of fungal siderophore biosynthesis and uptake: The role of siderophores in iron uptake and storage ' (2003 ) 62 Appl Microbiol Biotechnol : 316 -330.

    • Search Google Scholar
  • Weinberg, E.D.: The development of awareness of iron-withholding defense. Perspect Biol Med 36, 215–221 (1993).

    Weinberg E.D. , 'The development of awareness of iron-withholding defense ' (1993 ) 36 Perspect Biol Med : 215 -221.

    • Search Google Scholar
  • Weinberg, E.D.: The role of iron in protozoan and fungal infectious diseases. J Eukaryot Microbiol 46, 231–238 (1999).

    Weinberg E.D. , 'The role of iron in protozoan and fungal infectious diseases ' (1999 ) 46 J Eukaryot Microbiol : 231 -238.

    • Search Google Scholar
  • Weiss, G.: Iron and immunity: A double-edged sword. Eur J Clin Invest 32 (Suppl. 1), 70–78 (2002).

    Weiss G. , 'Iron and immunity: A double-edged sword ' (2002 ) 32 Eur J Clin Invest : 70 -78.

  • Fluckinger, M., Haas, H., Merschak, P., Glasgow, B.J., Redl, B.: Human tear lipocalin exhibits antimicrobial activity by scavenging microbial siderophores. Antimicrob Agents Chemother 48, 3367–3372 (2004).

    Redl B. , 'Human tear lipocalin exhibits antimicrobial activity by scavenging microbial siderophores ' (2004 ) 48 Antimicrob Agents Chemother : 3367 -3372.

    • Search Google Scholar
  • Schrettl, M., Bignell, E., Kragl, C., Joechl, C., Rogers, T., Arst, H.N., Jr, Haynes, K., Haas, H.: Siderophore biosynthesis but not reductive iron assimilation is essential for Aspergillus fumigatus virulence. J Exp Med 200, 1213–1219 (2004).

    Haas H. , 'Siderophore biosynthesis but not reductive iron assimilation is essential for Aspergillus fumigatus virulence ' (2004 ) 200 J Exp Med : 1213 -1219.

    • Search Google Scholar
  • Schrettl, M., Bignell, E., Kragl, C., Sabiha, Y., Loss, O., Eisendle, M., Wallner, A., Arst, Jr, H. N., Haynes, K., Haas, H.: Distinct roles for intra- and extracellular siderophores during Aspergillus fumigatus infection. PLoS Pathog 3, 1195–1207 (2007).

    Haas H. , 'Distinct roles for intra- and extracellular siderophores during Aspergillus fumigatus infection ' (2007 ) 3 PLoS Pathog : 1195 -1207.

    • Search Google Scholar
  • Hissen, A.H., Wan, A.N., Warwas, M.L., Pinto, L.J., Moore, M.M.: The Aspergillus fumigatus siderophore biosynthetic gene sidA, encoding 1-ornithine N5-oxygenase, is required for virulence. Infect Immun 73, 5493–5503 (2005).

    Moore M.M. , 'The Aspergillus fumigatus siderophore biosynthetic gene sidA, encoding 1-ornithine N5-oxygenase, is required for virulence ' (2005 ) 73 Infect Immun : 5493 -5503.

    • Search Google Scholar
  • Barratt, R.W., Johnson, G.B., Ogata, W.N.: Wild-type and mutant stocks of Aspergillus nidulans. Genetics 52, 233–246 (1965).

    Ogata W.N. , 'Wild-type and mutant stocks of Aspergillus nidulans ' (1965 ) 52 Genetics : 233 -246.

    • Search Google Scholar
  • Balázs, A., Pócsi, I., Hamari, Zs., Leiter, É., Emri, T., Miskei, M., Oláh, J., Tóth, V., Hegedűs, N., Prade, R.F., Molnár, M., Pócsi, I.: AtfA bZIP-type transcription factor regulates oxidative and osmotic stress responses in Aspergillus nidulans. Mol Genet Genomics 283, 289–303 (2010).

    Pócsi I. , 'AtfA bZIP-type transcription factor regulates oxidative and osmotic stress responses in Aspergillus nidulans ' (2010 ) 283 Mol Genet Genomics : 289 -303.

    • Search Google Scholar
  • Hördt, W., Römheld, V., Winkelmann, G.: Fusarinines and dimerumacid, mono- and dihydroxamate siderophores from Penicillium chrysogenum, improve iron utilization by strategy I and strategy II plants. Biometals 13(1), 37–46 (2000).

    Winkelmann G. , 'Fusarinines and dimerumacid, mono- and dihydroxamate siderophores from Penicillium chrysogenum, improve iron utilization by strategy I and strategy II plants ' (2000 ) 13 Biometals : 37 -46.

    • Search Google Scholar
  • Leiter, É., Emri, T., Nagy, I., Pócsi, I., Winkelmann, G., Pócsi, I.: Penicillin V Production by Penicillium chrysogenum in the presence of Fe3+ and in low-iron culture medium. Folia Microbiol 46, 127–132 (2001).

    Pócsi I. , 'Penicillin V Production by Penicillium chrysogenum in the presence of Fe3+ and in low-iron culture medium ' (2001 ) 46 Folia Microbiol : 127 -132.

    • Search Google Scholar
  • Pócsi, I., Jeney, V., Kertai, P., Pócsi, I., Gyémánt, G., Fésüs, L., Balla, J., Balla, G.: Fungal siderophores function as protective agents of LDL oxidation and are promising anti-atherosclerotic metabolites in functional food. Mol Nutr Food Res 52(12), 1434–1447 (2008).

    Balla G. , 'Fungal siderophores function as protective agents of LDL oxidation and are promising anti-atherosclerotic metabolites in functional food ' (2008 ) 52 Mol Nutr Food Res : 1434 -1447.

    • Search Google Scholar
  • Toth, V., Antal, K., Gyemant, Gy., Miskei, M., Pocsi, I., Emri T.: Optimization of coprogen production in Neurospora crassa. Acta Biol Hung 60, 321–328 (2009).

    Emri T. , 'Optimization of coprogen production in Neurospora crassa ' (2009 ) 60 Acta Biol Hung : 321 -328.

    • Search Google Scholar
  • Emri, T., Tóth, V., Nagy, Cs.T., Nagy, G., Pócsi, I., Gyémánt, G., Antal, K., Balla, J., Balla, G., Román, G., Kovács, I., Pócsi, I.: Towards high-siderophore-content foods: Optimization of coprogens production in submerged cultures of Penicillium nalgiovense. J Sci Food Agric 93, 2221–2228 (2013).

    Pócsi I. , 'Towards high-siderophore-content foods: Optimization of coprogens production in submerged cultures of Penicillium nalgiovense ' (2013 ) 93 J Sci Food Agric : 2221 -2228.

    • Search Google Scholar
  • Mortazavi, M., Akbarzadeh, A.: Improvement of desferrioxamine B production of Streptomyces pilosus ATCC 19797 with use of protease inhibitor and minerals related to its activity. Ind J Clinic Biochem 27(3), 274–277 (2012).

    Akbarzadeh A. , 'Improvement of desferrioxamine B production of Streptomyces pilosus ATCC 19797 with use of protease inhibitor and minerals related to its activity ' (2012 ) 27 Ind J Clinic Biochem : 274 -277.

    • Search Google Scholar
  • Calvente, V., de Orellano, M. E., Sansone, G., Benuzzi, D., Sanz de Tosetti, M.I.: Effect of nitrogen source and pH on siderophore production by Rhodotorula strains and their application to biocontrol of phytopathogenic moulds. J Ind Microbiol. Biotechnol 26, 226–229 (2001).

    Sanz de Tosetti M.I. , 'Effect of nitrogen source and pH on siderophore production by Rhodotorula strains and their application to biocontrol of phytopathogenic moulds ' (2001 ) 26 J Ind Microbiol. Biotechnol : 226 -229.

    • Search Google Scholar
  • Eisendle, M., Oberegger, H., Buttinger, R., Illmer, P., Haas, H.: Biosynthesis and uptake of siderophores is controlled by the PacC-mediated ambient pH regulatory system in Aspergillus nidulans. Eukaryotic Cell 3, 561–563 (2004).

    Haas H. , 'Biosynthesis and uptake of siderophores is controlled by the PacC-mediated ambient pH regulatory system in Aspergillus nidulans ' (2004 ) 3 Eukaryotic Cell : 561 -563.

    • Search Google Scholar
  • Federspiel, A., Schuler, R., Haselwandter, K.: Effect of pH, L-ornithine and L-proline on the hydroxamate siderophore production by Hymenoscyphus ericae, a typical ericoid mycorrhizal fungus. Plant and Soil 130, 259–261 (1991).

    Haselwandter K. , 'Effect of pH, L-ornithine and L-proline on the hydroxamate siderophore production by Hymenoscyphus ericae, a typical ericoid mycorrhizal fungus ' (1991 ) 130 Plant and Soil : 259 -261.

    • Search Google Scholar
  • Kosman, D.J.: Molecular mechanisms of iron uptake in fungi. Molecular Microbiology 47, 1185–1197 (2003).

    Kosman D.J. , 'Molecular mechanisms of iron uptake in fungi ' (2003 ) 47 Molecular Microbiology : 1185 -1197.

    • Search Google Scholar
  • Schrettl, M., Ibrahim-Granet, O., Droin, S., Huerre, M., Latgé, J.P., Haas, H.: The crucial role of the Aspergillus fumigatus siderophore system in interaction with alveolar macrophages. Microbes and Infections 12, 1035–1041 (2010).

    Haas H. , 'The crucial role of the Aspergillus fumigatus siderophore system in interaction with alveolar macrophages ' (2010 ) 12 Microbes and Infections : 1035 -1041.

    • Search Google Scholar
  • Seifert, M., Nairz, M., Schroll, A., Schrettl, M., Haas, H., Weiss, G.: Affects of Aspergillus fumigatus siderophore system on the regulation of macrophage immune effector pathways and iron homeostasis. Immunbiology 213, 767–778 (2008).

    Weiss G. , 'Affects of Aspergillus fumigatus siderophore system on the regulation of macrophage immune effector pathways and iron homeostasis ' (2008 ) 213 Immunbiology : 767 -778.

    • Search Google Scholar
  • Jahn, B., Langfelder, K., Schneider, U., Schneidel, C., Brakhage, A.A.: PKSP-dependent reduction of phagolysosome fusion and intracellular kill of Aspergillus fumigatus conidia by human monocyte-derived macrophages. Cell Microbiol 4(12), 792–803 (2002).

    Brakhage A.A. , 'PKSP-dependent reduction of phagolysosome fusion and intracellular kill of Aspergillus fumigatus conidia by human monocyte-derived macrophages ' (2002 ) 4 Cell Microbiol : 792 -803.

    • Search Google Scholar
  • Slesiona, S., Gressler, M., Mihlan, M., Zaehle, C., Schaller, M., Barz, D., Hube, B., Jacobsen, I.D., Brock, M.: Persistence versus escape: Aspergillus terreus and Aspergillus fumigatus employ different strategies during interactions with macrophages. Plos One 7, e31223 (2012).

    Brock M. , 'Persistence versus escape: Aspergillus terreus and Aspergillus fumigatus employ different strategies during interactions with macrophages ' (2012 ) 7 Plos One : e31223 -.

    • Search Google Scholar
  • Ibrahim-Granet, O., Philippe, B., Boleti, H., Boisvieux-Ulrich, E., Grenet, D., Stern, M., Latigé, J.P.: Phagocytosis and intracellular fate of Aspergillus fumigatus conidia in alveolar macrophages. Inf Im 71, 891–903 (2003).

    Latigé J.P. , 'Phagocytosis and intracellular fate of Aspergillus fumigatus conidia in alveolar macrophages ' (2003 ) 71 Inf Im : 891 -903.

    • Search Google Scholar
  • Seifert, M., Nairz, M., Schroll, A., Schrettl, M., Haas, H., Weiss, G.: Affects of Aspergillus fumigatus siderophore system on the regulation of macrophage immune effector pathways and iron homeostasis. Immunbiology 213, 767–778 (2008).

    Weiss G. , 'Affects of Aspergillus fumigatus siderophore system on the regulation of macrophage immune effector pathways and iron homeostasis ' (2008 ) 213 Immunbiology : 767 -778.

    • Search Google Scholar
  • Eisendle, M., Oberegger, H., Zadra, I., Haas, H.: The siderophore system is essential for viability of Aspergillus nidulans: functional analysis of two genes encoding l-ornithine N 5-monooxygenase (sidA) and a non-ribosomal peptidesynthetase (sidC). Mol. Microbiol 49, 359–375 (2003).

    Haas H. , 'The siderophore system is essential for viability of Aspergillus nidulans: functional analysis of two genes encoding l-ornithine N 5-monooxygenase (sidA) and a non-ribosomal peptidesynthetase (sidC) ' (2003 ) 49 Mol. Microbiol : 359 -375.

    • Search Google Scholar
  • Haas, H., Eisendle, M., Turgeon, B.G.: Siderophores in fungal physiology and virulence. Annu. Rev. Phytopathol 46, 149–187 (2008).

    Turgeon B.G. , 'Siderophores in fungal physiology and virulence ' (2008 ) 46 Annu. Rev. Phytopathol : 149 -187.

    • Search Google Scholar
  • Davis, R.H.: Compartmental and regulatory mechanisms in the arginine pathways of Neurospora crassa and Sacharomyces cerevisiae. Microbial Rev 50, 280–313 (1986).

    Davis R.H. , 'Compartmental and regulatory mechanisms in the arginine pathways of Neurospora crassa and Sacharomyces cerevisiae ' (1986 ) 50 Microbial Rev : 280 -313.

    • Search Google Scholar
  • Schrettl, M., Kim, H.S., Eisendle, M., Kragl, C., Nierman, W.C., Heinekamp, T., Werner, E.R., Jacobsen, I., Illmer, P., Yi, H., Brakhage, A.A., Haas, H.: SreA-mediated iron regulation in Aspergillus fumigatus. Mol Microbiol 70, 27–43 (2008).

    Haas H. , 'SreA-mediated iron regulation in Aspergillus fumigatus ' (2008 ) 70 Mol Microbiol : 27 -43.

    • Search Google Scholar

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