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  • 1 Baqiyatallah University of Medical Sciences Molecular Biology Research Center Tehran Iran
  • | 2 Shiraz University of Medical Sciences Prof. Alborzi Clinical Microbiology Research Center Shiraz Iran
  • | 3 Baqiyatallah University of Medical Sciences Applied Virology Research Center Tehran Iran
  • | 4 Ilam University of Medical Sciences Clinical Microbiology Research Center Ilam Iran
  • | 5 Department of Sciences for Health Promotion and Mother-Child Care ‘G. D’Alessandro’, University Palermo Italy
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We evaluated the performances of a newly designed real-time polymerase chain reaction (PCR) assay using TaqMan® probes to detect Salmonella Typhi. TaqMan® real-time PCR assays were performed by designed primers and probe based on the staG gene for detecting S. Typhi. The specificity of the assay was evaluated on 15 Salmonella serovars. The analytical specificity was evaluated on 20 non-Salmonella microorganisms. The analytical sensitivity was assessed using decreasing DNA quantities of S. Typhi ATCC 19430. Finally the detection capability of the TaqMan® real-time PCR assay on isolates recovered from patients with Salmonella infections was compared to the conventional PCR assay. Only S. Typhi strain had positive results when subjected to the assay using Typhi-specific real-time PCR. No amplification products were observed in real-time PCR with any of the non-Salmonella microorganisms tested. The TaqMan® real-time PCR was more sensitive than the conventional PCR. In conclusion, we found that the easy-to-use real-time PCR assays were faster than conventional PCR systems. The staG-based TaqMan® real-time PCR assay showed to be specific and sensitive method for the safe and rapid detection of the S. Typhi.

  • Hald, T., Lo Fo Wong, D.M., Aarestrup, F.M.: The attribution of human infections with antimicrobial resistant Salmonella bacteria in Denmark to sources of animal origin. Food-Borne Patho Dis 4, 313–326 (2007).

    Aarestrup F.M. , 'The attribution of human infections with antimicrobial resistant Salmonella bacteria in Denmark to sources of animal origin ' (2007 ) 4 Food-Borne Patho Dis : 313 -326.

    • Search Google Scholar
  • Naghoni, A., Ranjbar, R., Tabaraie, B., Farshad, S., Owlia, P., Safiri, Z., Mammina, C.: High prevalence of integron-mediated resistances among clinical isolates of Salmonella enterica. Jpn J of Infect Dis 63, 417–421 (2010).

    Mammina C. , 'High prevalence of integron-mediated resistances among clinical isolates of Salmonella enterica ' (2010 ) 63 Jpn J of Infect Dis : 417 -421.

    • Search Google Scholar
  • World Health Organization: World Health Organization antimicrobial resistance fact sheet no. 139. Available from http://www.who.int/mediacentre/factsheets/fs139 (2003)

    '', in World Health Organization antimicrobial resistance fact sheet no. 139 , (2003 ) -.

  • Karami, A., Ranjbar, R., Ahmadi, Z., Safiri, Z.: Rapid detection of different serovares of Salmonella enterica by multiplex PCR. Iran J Public Health 36, 38–42 (2007).

    Safiri Z. , 'Rapid detection of different serovares of Salmonella enterica by multiplex PCR ' (2007 ) 36 Iran J Public Health : 38 -42.

    • Search Google Scholar
  • Liming, S.H., Bhagwat, A.A.: Application of a molecular beacon — real-time PCR technology to detect Salmonella species contaminating fruits and vegetables. Int J Food Microbiol 95, 177–187 (2004).

    Bhagwat A.A. , 'Application of a molecular beacon — real-time PCR technology to detect Salmonella species contaminating fruits and vegetables ' (2004 ) 95 Int J Food Microbiol : 177 -187.

    • Search Google Scholar
  • Bohaychuk, V.M., Gensler, G.E., McFall, M.E., King, R.K., Renter, D.G.: A real-time PCR assay for the detection of Salmonella in a wide variety of food and food-animal matrices. J Food Prot 70, 1080–1087 (2007).

    Renter D.G. , 'A real-time PCR assay for the detection of Salmonella in a wide variety of food and food-animal matrices ' (2007 ) 70 J Food Prot : 1080 -1087.

    • Search Google Scholar
  • Feng, P.: Impact of molecular biology on the detection of foodborne pathogens. Mol Biotechnol 7, 267–278 (1997).

    Feng P. , 'Impact of molecular biology on the detection of foodborne pathogens ' (1997 ) 7 Mol Biotechnol : 267 -278.

    • Search Google Scholar
  • Kurowski, P.B., Traub-Dargatz, J.L., Morley, P.S., Gentry-Weeks, C.R.: Detection of Salmonella spp in fecal specimens by use of real-time polymerase chain reaction assay. Am J Vet Res 63, 1265–1268 (2002).

    Gentry-Weeks C.R. , 'Detection of Salmonella spp in fecal specimens by use of real-time polymerase chain reaction assay ' (2002 ) 63 Am J Vet Res : 1265 -1268.

    • Search Google Scholar
  • Chen, J., Zhang, L., Paoli, G.C.: A real-time PCR method for the detection of Salmonella enterica from food using a target sequence identified by comparative genomic analysis. Int J Food Microbiol 137, 168–174 (2010).

    Paoli G.C. , 'A real-time PCR method for the detection of Salmonella enterica from food using a target sequence identified by comparative genomic analysis ' (2010 ) 137 Int J Food Microbiol : 168 -174.

    • Search Google Scholar
  • Li, X., Boudjellab, N., Zhao, X.: Combined PCR and slot blot assay for detection of Salmonella and Listeria monocytogenes. Int J Food Microbiol 56, 167–177 (2000).

    Zhao X. , 'Combined PCR and slot blot assay for detection of Salmonella and Listeria monocytogenes ' (2000 ) 56 Int J Food Microbiol : 167 -177.

    • Search Google Scholar
  • Fratamico, P., Strobaugh, T.P.: Simultaneous detection of Salmonella spp. and Escherichia coli O157:H7 by multiplex PCR. J Ind Microbiol Biotech 21, 92–98 (1998).

    Strobaugh T.P. , 'Simultaneous detection of Salmonella spp. and Escherichia coli O157:H7 by multiplex PCR ' (1998 ) 21 J Ind Microbiol Biotech : 92 -98.

    • Search Google Scholar
  • Chen, W., Martinez, G., Mulchandani, A.: Molecular beacons: A real-time polymerase chain reaction assay for detecting Salmonella. Anal Biochem 280, 166–172 (2000).

    Mulchandani A. , 'Molecular beacons: A real-time polymerase chain reaction assay for detecting Salmonella ' (2000 ) 280 Anal Biochem : 166 -172.

    • Search Google Scholar
  • Hoorfar, J., Radstrem, P.: Automated 5′ nuclease PCR assay for identification of Salmonella enterica. J Clin Microbiol 38, 3429–3435 (2000).

    Radstrem P. , 'Automated 5′ nuclease PCR assay for identification of Salmonella enterica ' (2000 ) 38 J Clin Microbiol : 3429 -3435.

    • Search Google Scholar
  • Tyagi, S., Kramer, F.R.: Molecular beacon: Probes that fluoresce upon hybridization. Nat Biotechnol 14, 303–308 (1996).

    Kramer F.R. , 'Molecular beacon: Probes that fluoresce upon hybridization ' (1996 ) 14 Nat Biotechnol : 303 -308.

    • Search Google Scholar
  • Chen, S., Yee, A., Griffiths, M., Larkin, C., Yamashiro, C.T., Behari, R., Paszko-Kolva, C., Rahn, K., De Grandis, S.A.: The evaluation of a fluorogenic polymerase chain reaction assay for the detection of Salmonella species in food commodities. Int J Food Microbiol 35, 239–250 (1997).

    Grandis S.A. , 'The evaluation of a fluorogenic polymerase chain reaction assay for the detection of Salmonella species in food commodities ' (1997 ) 35 Int J Food Microbiol : 239 -250.

    • Search Google Scholar
  • McKllip, J.L., Drake, M.: Molecular beacon polymerase chain reaction detection of Escherichia coli O157:H7 in milk. J Food Prot 63, 855–859 (2000).

    Drake M. , 'Molecular beacon polymerase chain reaction detection of Escherichia coli O157:H7 in milk ' (2000 ) 63 J Food Prot : 855 -859.

    • Search Google Scholar
  • Bounaadja, L., Albert, D., Chenais, B., Henault, S., Zygmunt, M.S., Poliak, S., Garin-Bastuji, B.: Real-time PCR for identification of Brucella spp.: A comparative study of IS711, bcsp31 and per target genes. Vet Microbiol 137, 156–164 (2009).

    Garin-Bastuji B. , 'Real-time PCR for identification of Brucella spp.: A comparative study of IS711, bcsp31 and per target genes ' (2009 ) 137 Vet Microbiol : 156 -164.

    • Search Google Scholar
  • Arya, M., Shergill, I.S., Williamson, M., Gommersall, L., Arya, N., Patel, H.R.: Basic principles of real-time quantitative PCR. Expert Rev Mol Diagn 5, 209–219 (2005).

    Patel H.R. , 'Basic principles of real-time quantitative PCR ' (2005 ) 5 Expert Rev Mol Diagn : 209 -219.

    • Search Google Scholar
  • Smith, C.J., Osborn, A.M.: Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. FEMS Microbiol Ecol 67, 6–20 (2009).

    Osborn A.M. , 'Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology ' (2009 ) 67 FEMS Microbiol Ecol : 6 -20.

    • Search Google Scholar
  • Farrell, J.J., Doyle, L.J., Addison, R.M., Reller, L.B., Hall, G.S., Procop, G.W.: Broad-range (Pan) Salmonella and Salmonella serotype Typhi-specific real-time PCR assays. Am J Clin Pathol 123, 339–345 (2005).

    Procop G.W. , 'Broad-range (Pan) Salmonella and Salmonella serotype Typhi-specific real-time PCR assays ' (2005 ) 123 Am J Clin Pathol : 339 -345.

    • Search Google Scholar
  • Van Kessel, J.S., Karns, J.S., Perdue, M.L.: Using a portable real-time PCR assay to detect Salmonella in raw milk. J Food Prot 66, 1762–1767 (2003).

    Perdue M.L. , 'Using a portable real-time PCR assay to detect Salmonella in raw milk ' (2003 ) 66 J Food Prot : 1762 -1767.

    • Search Google Scholar
  • Nga, T.V., Karkey, A., Dongol, S., Thuy, H.N., Dunstan, S., Holt, K., Tu le, T. P., Campbell, J.I., Chau, T.T., Chau, N.V., Arjyal, A., Koirala, S., Basnyat, B., Dolecek, C., Farrar, J., Baker, S.: The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens. BMC Infect Dis 10, 125 (2010).

    Baker S. , 'The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens ' (2010 ) 10 BMC Infect Dis : 125 -.

    • Search Google Scholar
  • Jordan, R., van Heerden, E., Hugo, C.J. Piater, L.A.: Using current molecular techniques for rapid differentiation of Salmonella Typhi and Salmonella Typhimurium. Afr J Biotechnol 8, 1815–1818 (2009).

    Piater L.A. , 'Using current molecular techniques for rapid differentiation of Salmonella Typhi and Salmonella Typhimurium ' (2009 ) 8 Afr J Biotechnol : 1815 -1818.

    • Search Google Scholar
  • Hashimoto, Y., Itho, Y., Fujinaga, Y., Khan, A.Q., Sultana, F., Miyake, M., Hirose, K., Yamamoto, H., Ezaki, T.: Development of nested PCR based on the ViaB sequence to detect Salmonella typhi. J Clin Microbiol 33, 775–777 (1995).

    Ezaki T. , 'Development of nested PCR based on the ViaB sequence to detect Salmonella typhi ' (1995 ) 33 J Clin Microbiol : 775 -777.

    • Search Google Scholar
  • Moussa, I.M., Gassem, M.A., Al-Doss, A.A., Mahmoud, W.A., Sadik Abdel Mawgood, A.L.: Using molecular techniques for rapid detection of Salmonella serovars in frozen chicken and chicken products collected from Riyadh, Saudi Arabia. Afr J Biotechnol 9, 612–619 (2010).

    Sadik Abdel Mawgood A.L. , 'Using molecular techniques for rapid detection of Salmonella serovars in frozen chicken and chicken products collected from Riyadh, Saudi Arabia ' (2010 ) 9 Afr J Biotechnol : 612 -619.

    • Search Google Scholar
  • Anderson, A., Pietsch, K., Zucker, R., Mayr, A., Müller-Hohe, E., Messelhäusser, U., Sing, A., Busch, U., Huber, I.: Validation of a duplex real-time PCR for the detection of Salmonella spp. in different food products. Food Anal Methods 4, 259–267 (2011).

    Huber I. , 'Validation of a duplex real-time PCR for the detection of Salmonella spp. in different food products ' (2011 ) 4 Food Anal Methods : 259 -267.

    • Search Google Scholar

 

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Senior editors

Editor-in-Chief: Prof. Dóra Szabó (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Managing Editor: Dr. Béla Kocsis (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Co-editor: Dr. Andrea Horváth (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Editorial Board

  • Prof. Éva ÁDÁM (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Sebastian AMYES (Department of Medical Microbiology, University of Edinburgh, Edinburgh, UK.)
  • Dr. Katalin BURIÁN (Institute of Clinical Microbiology University of Szeged, Szeged, Hungary; Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.)
  • Dr. Orsolya DOBAY (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Ildikó Rita DUNAY (Institute of Inflammation and Neurodegeneration, Medical Faculty, Otto-von-Guericke University, Magdeburg, Germany; Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany)
  • Prof. Levente EMŐDY(Department of Medical Microbiology and Immunology, University of Pécs, Pécs, Hungary.)
  • Prof. Anna ERDEI (Department of Immunology, Eötvös Loránd University, Budapest, Hungary, MTA-ELTE Immunology Research Group, Eötvös Loránd University, Budapest, Hungary.)
  • Prof. Éva Mária FENYŐ (Division of Medical Microbiology, University of Lund, Lund, Sweden)
  • Prof. László FODOR (Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary)
  • Prof. József KÓNYA (Department of Medical Microbiology, University of Debrecen, Debrecen, Hungary)
  • Prof. Yvette MÁNDI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Károly MÁRIALIGETI (Department of Microbiology, Eötvös Loránd University, Budapest, Hungary)
  • Prof. János MINÁROVITS (Department of Oral Biology and Experimental Dental Research, University of Szeged, Szeged, Hungary)
  • Prof. Béla NAGY (Centre for Agricultural Research, Institute for Veterinary Medical Research, Budapest, Hungary.)
  • Prof. István NÁSZ (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Kristóf NÉKÁM (Hospital of the Hospitaller Brothers in Buda, Budapest, Hungary.)
  • Dr. Eszter OSTORHÁZI (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Rozália PUSZTAI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Peter L. RÁDY (Department of Dermatology, University of Texas, Houston, Texas, USA)
  • Prof. Éva RAJNAVÖLGYI (Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary)
  • Prof. Ferenc ROZGONYI (Institute of Laboratory Medicine, Semmelweis University, Budapest, Hungary)
  • Prof. Zsuzsanna SCHAFF (2nd Department of Pathology, Semmelweis University, Budapest, Hungary)
  • Prof. Joseph G. SINKOVICS (The Cancer Institute, St. Joseph’s Hospital, Tampa, Florida, USA)
  • Prof. Júlia SZEKERES (Department of Medical Biology, University of Pécs, Pécs, Hungary.)
  • Prof. Mária TAKÁCS (National Reference Laboratory for Viral Zoonoses, National Public Health Center, Budapest, Hungary.)
  • Prof. Edit URBÁN (Department of Medical Microbiology and Immunology University of Pécs, Pécs, Hungary; Institute of Translational Medicine, University of Pécs, Pécs, Hungary.)

 

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2020  
Total Cites 662
WoS
Journal
Impact Factor
2,048
Rank by Immunology 145/162(Q4)
Impact Factor Microbiology 118/137 (Q4)
Impact Factor 1,904
without
Journal Self Cites
5 Year 0,671
Impact Factor
Journal  0,38
Citation Indicator  
Rank by Journal  Immunology 146/174 (Q4)
Citation Indicator  Microbiology 120/142 (Q4)
Citable 42
Items
Total 40
Articles
Total 2
Reviews
Scimago 28
H-index
Scimago 0,439
Journal Rank
Scimago Immunology and Microbiology (miscellaneous) Q4
Quartile Score Medicine (miscellaneous) Q3
Scopus 438/167=2,6
Scite Score  
Scopus General Immunology and Microbiology 31/45 (Q3)
Scite Score Rank  
Scopus 0,760
SNIP
Days from  225
submission
to acceptance
Days from  118
acceptance
to publication
Acceptance 19%
Rate

2019  
Total Cites
WoS
485
Impact Factor 1,086
Impact Factor
without
Journal Self Cites
0,864
5 Year
Impact Factor
1,233
Immediacy
Index
0,286
Citable
Items
42
Total
Articles
40
Total
Reviews
2
Cited
Half-Life
5,8
Citing
Half-Life
7,7
Eigenfactor
Score
0,00059
Article Influence
Score
0,246
% Articles
in
Citable Items
95,24
Normalized
Eigenfactor
0,07317
Average
IF
Percentile
7,690
Scimago
H-index
27
Scimago
Journal Rank
0,352
Scopus
Scite Score
320/161=2
Scopus
Scite Score Rank
General Immunology and Microbiology 35/45 (Q4)
Scopus
SNIP
0,492
Acceptance
Rate
16%

 

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Acta Microbiologica et Immunologica Hungarica
Language English
Size A4
Year of
Foundation
1954
Publication
Programme
2021 Volume 68
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per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
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Address
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ISSN 1217-8950 (Print)
ISSN 1588-2640 (Online)

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