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  • 1 Department of Microbiology of Clinical Center of Serbia, Belgrade, Serbia
  • 2 Clinic for Infectious and Tropical Diseases of Clinical Center of Serbia, Belgrade, Serbia
  • 3 School of Medicine, Belgrade, Serbia
  • 4 Department of Hospital Epidemiology of Clinical Center of Serbia, Belgrade, Serbia
  • 5 Center for Preventive Medical Care Belgrade, Belgrade, Serbia
  • 6 Institute of Meat Hygiene and Technology, Belgrade, Serbia
  • 7 Henry Ford Hospital, Detroit, Michigan, USA
  • 8 Wayne State University School of Medicine, Detroit, Michigan, USA
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In this study the distribution of species and antimicrobial resistance among vancomycin resistant enterococci (VRE) recovered from clinical specimens obtained from five hospitals in Belgrade was analyzed. Strains were further characterized by pulsed-field gel electrophoresis (PFGE). Polymerase chain reaction (PCR) was used to investigate the presence of vanA and vanB genes and pathogenicity factor genes. Identification of 194 VRE isolates revealed 154 Enterococcus faecium, 21 Enterococcus faecalis, 10 Enterococcus raffinosus and 9 Enterococcus gallinarum. This study revealed existence of 8 major clones of VRE. PCR determined vanA gene to be present in all of the VRE studied. Esp and hyl genes were present in 29.22% and 27.92% of E. faecium, respectively, and in 76.19% and 0 of E. faecalis, respectively. Esp and hyl genes were not found more frequently in members of predominant clones of E. faecium than in single isolates; nor was their presence connected to invasiveness.

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