View More View Less
  • 1 Hungarian Academy of Sciences, Budapest, Hungary
  • | 2 University of Pécs, Pécs, Hungary
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $784.00

Cytolethal distending toxins (CDT) represent an emerging toxin family, widely distributed among pathogenic bacteria. The cdtABC genes in E. coli are either part of the genome of prophages, plasmid or pathogenicity island. In order to investigate the stability and the transfer potential of cdt-IV genes cdtB gene was replaced by chloramphenicol (Cm) resistance encoding cat gene in the avian pathogenic E. coli (APEC) strain E250. After consecutive passages in non-selective medium at 37 °C 7.6% (219/2900) of the investigated colonies of E250::cat strain became Cm-sensitive (CmS). To reveal deletion mechanism 177 CmS colonies were investigated for presence of cdtA, cdtC and cdtC associated gene by PCR. One hundred and sixteen colonies of the CmS colonies (65.5%) showed partial or complete deletion in the cdt-IV region. Progressive loss of the upstream genes of the cdt cluster in E250 compared to other CDT-IV producing APEC strains and the fact that all the potential deletion patterns were identified, suggests the presence of an unstable hitherto unknown genomic region. The failure of in vitro transfer of cdt genes into a porcine EPEC E. coli strain suggests that the deletion of cdt-IV flanking genes alone do not promote the spread of cdt-IV.

  • 1.

    Kaper, J. B., Nataro, J. P., Mobley, H. L.: Pathogenic Escherichia coli. Nat Rev Microbiol 2, 123140 (2004).

  • 2.

    Hacker, J., Carniel, E.: Ecological fitness, genomic islands and bacterial pathogenicity. A Darwinian view of the evolution of microbes. EMBO Rep 2, 376381 (2001).

    • Search Google Scholar
    • Export Citation
  • 3.

    Dobrindt, U., Chowdary, M. G., Krumbholz, G., Hacker, J.: Genome dynamics and its impact on evolution of Escherichia coli. Med Microbiol Immunol 199, 145154 (2010).

    • Search Google Scholar
    • Export Citation
  • 4.

    Matsuda, M., Barksdale, L.: Phage directed synthesis of diphtherial toxin in non-toxigenic Corynebacterium diphtheriae. Nature 210, 911913 (1966).

    • Search Google Scholar
    • Export Citation
  • 5.

    O’Brien, A. D., Newland, J. W., Miller, S. F., Holmes, R. K., Smith, H. W., Formal, S. B.: Shiga-like toxin-converting phages from Escherichia coli strains that cause hemorrhagic colitis or infantile diarrhea. Science 226, 694696 (1984).

    • Search Google Scholar
    • Export Citation
  • 6.

    Brüssow, H., Canchaya, C., Hardt, W. D.: Phages and the evolution of bacterial pathogens: From genomic rearrangements to lysogenic conversion. Microbiol Mol Biol Rev 68, 560602 (2004).

    • Search Google Scholar
    • Export Citation
  • 7.

    Tóth, I., Sváb, D.: Cell cycle modulating toxins produced by Escherichia coli. In Morabito, S. (ed.): Pathogenic Escherichia coli. pp. 117138 (2014).

    • Search Google Scholar
    • Export Citation
  • 8.

    Asakura, M., Hinenoya, A., Alam, M. S., Shima, K., Zahid, S. H., Shi, L., Sugimoto, N., Ghosh, A. N., Ramamurthy, T., Faruque, S. M., Nair, G. B., Yamasaki S.: An inducible lambdoid prophage encoding cytolethal distending toxin (Cdt-I) and a type III effector protein in enteropathogenic Escherichia coli. Proc Natl Acad Sci U S A 104, 144831448 (2007).

    • Search Google Scholar
    • Export Citation
  • 9.

    Sváb, D., Horváth, B., Maróti, G., Dobrindt, U., Tóth, I.: Sequence variability of P2-like prophage genomes carrying the cytolethal distending toxin V operon in Escherichia coli O157. Appl Environ Microbiol 79, 49584964 (2013).

    • Search Google Scholar
    • Export Citation
  • 10.

    Pérès, S. Y., Marchès, O., Daigle, F., Nougayrède, J. P., Herault, F., Tasca, C., De Rycke, J., Oswald, E.: A new cytolethal distending toxin (CDT) from Escherichia coli producing CNF2 blocks HeLa cell division in G2/M phase. Mol Microbiol 24, 10951107 (1997).

    • Search Google Scholar
    • Export Citation
  • 11.

    Johnson, T. J., DebRoy, C., Belton, S., Williams, M. L., Lawrence, M., Nolan, L. K., Thorsness, J. L.: Pyrosequencing of the Vir plasmid of necrotoxigenic Escherichia coli. Vet Microbiol 144, 100109 (2010).

    • Search Google Scholar
    • Export Citation
  • 12.

    Tóth, I., Nougayrède, J. P., Dobrindt, U., Ledger, T. N., Boury, M., Morabito, S., Fujiwara, T., Sugai, M., Hacker, J., Oswald, E.: Cytolethal distending toxin type I and type IV genes are framed with lambdoid prophage genes in extraintestinal pathogenic Escherichia coli. Infect Immun 77, 492500 (2009).

    • Search Google Scholar
    • Export Citation
  • 13.

    Tóth, I., Dobrindt, U., Koscsó, B., Kósa, A., Herpay, M., Nagy, B.: Genetic and phylogenetic analysis of avian extraintestinal and intestinal Escherichia coli. Acta Microbiol Immunol Hung 59, 393409 (2012).

    • Search Google Scholar
    • Export Citation
  • 14.

    Bertani, G.: Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol 62, 293300 (1952).

  • 15.

    Schneider, G., Dobrindt, U., Middendorf, B., Hochhut, B., Szijártó, V., Emody, L., Hacker, J.: Mobilisation and remobilisation of a large archetypal pathogenicity island of uropathogenic Escherichia coli in vitro support the role of conjugation for horizontal transfer of genomic islands. BMC Microbiol 11, 210 (2011).

    • Search Google Scholar
    • Export Citation
  • 16.

    Tóth, I., Hérault, F., Beutin, L., Oswald, E.: Production of cytolethal distending toxins by pathogenic Escherichia coli strains isolated from human and animal sources: Establishment of the existence of a new cdt variant (Type IV). J Clin Microbiol 41, 42854291 (2003).

    • Search Google Scholar
    • Export Citation
  • 17.

    Miller, V. L., Mekalanos, J. J.: A novel suicide vector and its use in construction of insertion mutations: Osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholera requires toxR. J Bacteriol 170, 25752583 (1998).

    • Search Google Scholar
    • Export Citation
  • 18.

    Zhu, C., Harel, J., Jacques, M., Desautels, C., Donnenberg, M. S., Beaudry, M., Fairbrother, J. M.:. Virulence properties and attaching-effacing activity of Escherichia coli O45 from swine postweaning diarrhea. Infect Immun 62, 41534159 (1994).

    • Search Google Scholar
    • Export Citation
  • 19.

    Middendorf, B., Hochhut, B., Leipold, K., Dobrindt, U., Blum-Oehler, G., Hacker J.: Instability of pathogenicity islands in uropathogenic Escherichia coli 536. J Bacteriol 186, 30863096 (2004).

    • Search Google Scholar
    • Export Citation
  • 20.

    Middendorf, B., Blum-Oehler, G., Dobrindt, U., Mühldorfer, I., Salge, S., Hacker, J.: The pathogenicity islands (PAIs) of the uropathogenic Escherichia coli strain 536: Island probing of PAI II536. J Infect Dis 183 (Suppl1), S1720 (2001).

    • Search Google Scholar
    • Export Citation
  • 21.

    Schubert, S., Dufke, S., Sorsa, J., Heesemann J.: A novel integrative and conjugative element (ICE) of Escherichia coli: The putative progenitor of the Yersinia high-pathogenicity island. Mol Microbiol 51, 837848 (2004).

    • Search Google Scholar
    • Export Citation

 

The author instruction is available in PDF.
Please, download the file from HERE

Senior editors

Editor-in-Chief: Prof. Dóra Szabó (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Managing Editor: Dr. Béla Kocsis (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Co-editor: Dr. Andrea Horváth (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Editorial Board

  • Prof. Éva ÁDÁM (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Sebastian AMYES (Department of Medical Microbiology, University of Edinburgh, Edinburgh, UK.)
  • Dr. Katalin BURIÁN (Institute of Clinical Microbiology University of Szeged, Szeged, Hungary; Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.)
  • Dr. Orsolya DOBAY (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Ildikó Rita DUNAY (Institute of Inflammation and Neurodegeneration, Medical Faculty, Otto-von-Guericke University, Magdeburg, Germany; Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany)
  • Prof. Levente EMŐDY(Department of Medical Microbiology and Immunology, University of Pécs, Pécs, Hungary.)
  • Prof. Anna ERDEI (Department of Immunology, Eötvös Loránd University, Budapest, Hungary, MTA-ELTE Immunology Research Group, Eötvös Loránd University, Budapest, Hungary.)
  • Prof. Éva Mária FENYŐ (Division of Medical Microbiology, University of Lund, Lund, Sweden)
  • Prof. László FODOR (Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary)
  • Prof. József KÓNYA (Department of Medical Microbiology, University of Debrecen, Debrecen, Hungary)
  • Prof. Yvette MÁNDI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Károly MÁRIALIGETI (Department of Microbiology, Eötvös Loránd University, Budapest, Hungary)
  • Prof. János MINÁROVITS (Department of Oral Biology and Experimental Dental Research, University of Szeged, Szeged, Hungary)
  • Prof. Béla NAGY (Centre for Agricultural Research, Institute for Veterinary Medical Research, Budapest, Hungary.)
  • Prof. István NÁSZ (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Kristóf NÉKÁM (Hospital of the Hospitaller Brothers in Buda, Budapest, Hungary.)
  • Dr. Eszter OSTORHÁZI (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Rozália PUSZTAI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Peter L. RÁDY (Department of Dermatology, University of Texas, Houston, Texas, USA)
  • Prof. Éva RAJNAVÖLGYI (Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary)
  • Prof. Ferenc ROZGONYI (Institute of Laboratory Medicine, Semmelweis University, Budapest, Hungary)
  • Prof. Zsuzsanna SCHAFF (2nd Department of Pathology, Semmelweis University, Budapest, Hungary)
  • Prof. Joseph G. SINKOVICS (The Cancer Institute, St. Joseph’s Hospital, Tampa, Florida, USA)
  • Prof. Júlia SZEKERES (Department of Medical Biology, University of Pécs, Pécs, Hungary.)
  • Prof. Mária TAKÁCS (National Reference Laboratory for Viral Zoonoses, National Public Health Center, Budapest, Hungary.)
  • Prof. Edit URBÁN (Department of Medical Microbiology and Immunology University of Pécs, Pécs, Hungary; Institute of Translational Medicine, University of Pécs, Pécs, Hungary.)

 

Editorial Office:
Akadémiai Kiadó Zrt.
Budafoki út 187-187, A/3, H-1117 Budapest, Hungary

Editorial Correspondence:
Acta Microbiologica et Immunologica Hungarica
Institute of Medical Microbiology
Semmelweis University
P.O. Box 370
H-1445 Budapest, Hungary
Phone: + 36 1 459 1500 ext. 56101
Fax: (36 1) 210 2959
E-mail: amih@med.semmelweis-univ.hu

 Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • SCOPUS
  • Science Citation Index Expanded
2020  
Total Cites 662
WoS
Journal
Impact Factor
2,048
Rank by Immunology 145/162(Q4)
Impact Factor Microbiology 118/137 (Q4)
Impact Factor 1,904
without
Journal Self Cites
5 Year 0,671
Impact Factor
Journal  0,38
Citation Indicator  
Rank by Journal  Immunology 146/174 (Q4)
Citation Indicator  Microbiology 120/142 (Q4)
Citable 42
Items
Total 40
Articles
Total 2
Reviews
Scimago 28
H-index
Scimago 0,439
Journal Rank
Scimago Immunology and Microbiology (miscellaneous) Q4
Quartile Score Medicine (miscellaneous) Q3
Scopus 438/167=2,6
Scite Score  
Scopus General Immunology and Microbiology 31/45 (Q3)
Scite Score Rank  
Scopus 0,760
SNIP
Days from  225
sumbission
to acceptance
Days from  118
acceptance
to publication
Acceptance 19%
Rate

2019  
Total Cites
WoS
485
Impact Factor 1,086
Impact Factor
without
Journal Self Cites
0,864
5 Year
Impact Factor
1,233
Immediacy
Index
0,286
Citable
Items
42
Total
Articles
40
Total
Reviews
2
Cited
Half-Life
5,8
Citing
Half-Life
7,7
Eigenfactor
Score
0,00059
Article Influence
Score
0,246
% Articles
in
Citable Items
95,24
Normalized
Eigenfactor
0,07317
Average
IF
Percentile
7,690
Scimago
H-index
27
Scimago
Journal Rank
0,352
Scopus
Scite Score
320/161=2
Scopus
Scite Score Rank
General Immunology and Microbiology 35/45 (Q4)
Scopus
SNIP
0,492
Acceptance
Rate
16%

 

Acta Microbiologica et Immunologica Hungarica
Publication Model Online only Hybrid
Submission Fee none
Article Processing Charge 1100 EUR/article
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Online subsscription: 652 EUR / 812 USD
Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content.
Purchase per Title Individual articles are sold on the displayed price.

Acta Microbiologica et Immunologica Hungarica
Language English
Size A4
Year of
Foundation
1954
Publication
Programme
2021 Volume 68
Volumes
per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 1217-8950 (Print)
ISSN 1588-2640 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Feb 2021 4 1 2
Mar 2021 21 0 0
Apr 2021 23 0 0
May 2021 5 0 0
Jun 2021 7 1 1
Jul 2021 0 0 0
Aug 2021 0 0 0