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  • 1 PannonPharma Ltd., Hungary
  • | 2 University of Pécs, Hungary
  • | 3 Hungarian Academy of Sciences, Hungary
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Bactericidal effect against non-dividing bacteria is a very advantageous, but rare characteristic among antimicrobial agents, mostly possessed by those affecting the cell membrane. These kinds of agents can kill bacterial cells without lysis. We assessed these characteristics on primycin, a topical anti-staphylococcal agent highly effective against prevalent multiresistant strains, as it also acts on the cell membrane. In time-kill studies, primycin preserved its bactericidal activity against growth-arrested Staphylococcus aureus cultures. The bactericidal action was slower against growth-arrested cultures compared to the exponentially growing ones to different extents depending on the manner of arrest. The bactericidal effect was less influenced by stringent response and by protein synthesis inhibition, proving that it does not depend on metabolic activity. In contrast, uncoupling of the membrane potential predominantly slowed, and low temperature almost stopped killing of bacteria. In consideration of published data, these facts suggest that the antibacterial action of primycin involves disrupting of the membrane potential, and is predominantly influenced by the membrane fluidity. Optical density measurements and transmission electron microscopy verified that primycin kills bacterial cells without lysis. These results reveal favorable characteristics of primycin and point to, and broaden the knowledge on its membrane-targeted effect.

  • 1.

    Coates, A., Hu, Y., Bax, R., Page, C.: The future challenges facing the development of new antimicrobial drugs. Nat Rev Drug Discov 1, 895910 (2002).

  • 2.

    Mascio, C. T. M., Alder, J. D., Silverman, J. A.: Bactericidal action of daptomycin against stationary-phase and nondividing Staphylococcus aureus cells. Antimicrob Agents Chemother 51, 42554260 (2007).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 3.

    Ooi, N., Miller, K., Randall, C., Rhys-Williams, W., Love, W., Chopra, I.: XF-70 and XF-73, novel antibacterial agents active against slow-growing and non-dividing cultures of Staphylococcus aureus including biofilms. J Antimicrob Chemother 65, 7278 (2009).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 4.

    Cotroneo, N., Harris, R., Perlmutter, N., Beveridge, T., Silverman, J. A.: Daptomycin exerts bactericidal action without lysis of Staphylococcus aureus. Antimicrob Agents Chemother 52, 22232225 (2008).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 5.

    Vályi-Nagy, T., Úri, J., Szilágyi, I.: Primycin, a new antibiotic. Nature 174, 11051106 (1954).

  • 6.

    Horváth, I., Kramer, M., Bauer, P. I., Büki, K. G.: The mode of action of primycin. Arch Microbiol 121, 135139 (1979).

  • 7.

    Feiszt, P., Mestyán, G., Kerényi, M., Dobay, O., Szabo, J., Dombrádi, Z., Urbán, E., Emődy, L.: Re-evaluation of in vitro activity of primycin against prevalent multiresistant bacteria. Int J Med Microbiol 304, 10771085 (2014).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 8.

    Nyilasi, I., Kocsubé, S., Pesti, M., Lukács, G., Papp, T., Vágvölgyi, C.: In vitro interactions between primycin and different statins in their effects against some clinically important fungi. J Med Microbiol 59, 200205 (2010).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 9.

    Virág, E., Belagyi, J., Kocsubé, S., Vágvölgyi, C., Pesti, M.: Antifungal activity of the primycin complex and its main components A1, A2 and C1 on a Candida albicans clinical isolate, and their effects on the dynamic plasma membrane changes. J Antibiot (Tokyo) 66, 6772 (2013).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 10.

    Clinical and Laboratory Standards Institute (CLSI): Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline, M26-A. CLSI, Wayne, PA, USA, 1999.

    • Search Google Scholar
    • Export Citation
  • 11.

    Reiss, S., Pané-Farré, J., Fuchs, S., Francois, P., Liebeke, M., Schrenzel, J., Lindequist, U., Lalk, M., Wolz, C., Hecker, M., Engelmann, S.: Global analysis of the Staphylococcus aureus response to mupirocin. Antimicrob Agents Chemother 56, 787804 (2012).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 12.

    Yang, S. J., Xiong, Y. Q., Yeaman, M. R., Bayes, K. W., Adbelhady, W., Bayer, A. S.: Role of LytSR two-component regulatory system in adaptation to cationic antimicrobial peptides in Staphylococcus aureus. Antimicrob Agents Chemother 57, 38753882 (2013).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 13.

    Phadtare, S.: Recent developments in bacterial cold-shock response. Curr Issues Mol Biol 6, 125134 (2004).

  • 14.

    Virág, E., Juhász, Á., Kardos, R., Gazdag, Z., Papp, G., Pénzes, Á., Nyitrai, M., Vágvölgyi, C., Pesti, M.: In vivo direct interaction of the antibiotic primycin on a Candida albicans clinical isolate and its ergosterol-less mutant. Acta Biol Hung 63, 3851 (2012).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 15.

    Virág, E., Belágyi, J., Gazdag, Z., Vágvölgyi, C., Pesti, M.: Direct in vivo interaction of the antibiotic primycin with the plasma membrane of Candida albicans: An EPR study. Biochim Biophys Acta 1818, 4248 (2012).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 16.

    Xiong, Z., Ge, S., Chamberlain, N. R., Kapral, F. A.: Growth cycle-induced changes in sensitivity of Staphylococcus aureus to bactericidal lipids form abscesses. J Med Microbiol 39, 5863 (1993).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 17.

    Hurdle, J. G., O’Neill, A. J., Chopra, I., Lee, R. E.: Targeting bacterial membrane function: An underexploited mechanism for treating persistent infections. Nat Rev Microbiol 9, 6275 (2011).

    • Crossref
    • Search Google Scholar
    • Export Citation

 

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Senior editors

Editor-in-Chief: Prof. Dóra Szabó (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Managing Editor: Dr. Béla Kocsis (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Co-editor: Dr. Andrea Horváth (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Editorial Board

  • Prof. Éva ÁDÁM (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Sebastian AMYES (Department of Medical Microbiology, University of Edinburgh, Edinburgh, UK.)
  • Dr. Katalin BURIÁN (Institute of Clinical Microbiology University of Szeged, Szeged, Hungary; Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.)
  • Dr. Orsolya DOBAY (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Ildikó Rita DUNAY (Institute of Inflammation and Neurodegeneration, Medical Faculty, Otto-von-Guericke University, Magdeburg, Germany; Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany)
  • Prof. Levente EMŐDY(Department of Medical Microbiology and Immunology, University of Pécs, Pécs, Hungary.)
  • Prof. Anna ERDEI (Department of Immunology, Eötvös Loránd University, Budapest, Hungary, MTA-ELTE Immunology Research Group, Eötvös Loránd University, Budapest, Hungary.)
  • Prof. Éva Mária FENYŐ (Division of Medical Microbiology, University of Lund, Lund, Sweden)
  • Prof. László FODOR (Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary)
  • Prof. József KÓNYA (Department of Medical Microbiology, University of Debrecen, Debrecen, Hungary)
  • Prof. Yvette MÁNDI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Károly MÁRIALIGETI (Department of Microbiology, Eötvös Loránd University, Budapest, Hungary)
  • Prof. János MINÁROVITS (Department of Oral Biology and Experimental Dental Research, University of Szeged, Szeged, Hungary)
  • Prof. Béla NAGY (Centre for Agricultural Research, Institute for Veterinary Medical Research, Budapest, Hungary.)
  • Prof. István NÁSZ (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Kristóf NÉKÁM (Hospital of the Hospitaller Brothers in Buda, Budapest, Hungary.)
  • Dr. Eszter OSTORHÁZI (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Rozália PUSZTAI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Peter L. RÁDY (Department of Dermatology, University of Texas, Houston, Texas, USA)
  • Prof. Éva RAJNAVÖLGYI (Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary)
  • Prof. Ferenc ROZGONYI (Institute of Laboratory Medicine, Semmelweis University, Budapest, Hungary)
  • Prof. Zsuzsanna SCHAFF (2nd Department of Pathology, Semmelweis University, Budapest, Hungary)
  • Prof. Joseph G. SINKOVICS (The Cancer Institute, St. Joseph’s Hospital, Tampa, Florida, USA)
  • Prof. Júlia SZEKERES (Department of Medical Biology, University of Pécs, Pécs, Hungary.)
  • Prof. Mária TAKÁCS (National Reference Laboratory for Viral Zoonoses, National Public Health Center, Budapest, Hungary.)
  • Prof. Edit URBÁN (Department of Medical Microbiology and Immunology University of Pécs, Pécs, Hungary; Institute of Translational Medicine, University of Pécs, Pécs, Hungary.)

 

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2020  
Total Cites 662
WoS
Journal
Impact Factor
2,048
Rank by Immunology 145/162(Q4)
Impact Factor Microbiology 118/137 (Q4)
Impact Factor 1,904
without
Journal Self Cites
5 Year 0,671
Impact Factor
Journal  0,38
Citation Indicator  
Rank by Journal  Immunology 146/174 (Q4)
Citation Indicator  Microbiology 120/142 (Q4)
Citable 42
Items
Total 40
Articles
Total 2
Reviews
Scimago 28
H-index
Scimago 0,439
Journal Rank
Scimago Immunology and Microbiology (miscellaneous) Q4
Quartile Score Medicine (miscellaneous) Q3
Scopus 438/167=2,6
Scite Score  
Scopus General Immunology and Microbiology 31/45 (Q3)
Scite Score Rank  
Scopus 0,760
SNIP
Days from  225
sumbission
to acceptance
Days from  118
acceptance
to publication
Acceptance 19%
Rate

2019  
Total Cites
WoS
485
Impact Factor 1,086
Impact Factor
without
Journal Self Cites
0,864
5 Year
Impact Factor
1,233
Immediacy
Index
0,286
Citable
Items
42
Total
Articles
40
Total
Reviews
2
Cited
Half-Life
5,8
Citing
Half-Life
7,7
Eigenfactor
Score
0,00059
Article Influence
Score
0,246
% Articles
in
Citable Items
95,24
Normalized
Eigenfactor
0,07317
Average
IF
Percentile
7,690
Scimago
H-index
27
Scimago
Journal Rank
0,352
Scopus
Scite Score
320/161=2
Scopus
Scite Score Rank
General Immunology and Microbiology 35/45 (Q4)
Scopus
SNIP
0,492
Acceptance
Rate
16%

 

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Acta Microbiologica et Immunologica Hungarica
Language English
Size A4
Year of
Foundation
1954
Publication
Programme
2021 Volume 68
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per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
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Address
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Publisher Akadémiai Kiadó
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ISSN 1217-8950 (Print)
ISSN 1588-2640 (Online)

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