Although the antimicrobial activity of the engineered nanoparticles (NPs) is well known, the biochemical mechanisms underlying this activity are not clearly understood. Therefore, four NPs with the highest global production, namely SiO2, TiO2, ZnO, and Ag, were synthesized and characterized. The synthesized SiO2, TiO2, ZnO, and Ag NPs exhibit an average size of 11.12, 13.4, 35, and 50 nm, respectively. The antimicrobial activity of the synthesized NPs against bacteria and fungi were also determined. NPs-mediated inhibition of two very important enzymes, namely urease and DNA polymerase, is also reported. The synthesized NPs especially Ag and ZnO show significant antimicrobial activity against bacteria and fungi including methicillin-resistant Staphylococcus aureus even at low concentration. The DNA polymerase activity was inhibited at a very low concentration range of 2–4 µg/ml, whereas the urease activity was inhibited at a high concentration range of 50–100 µg/ml. Based on their ability to inhibit the urease and DNA polymerase, NPs can be arranged in the following order: Ag > ZnO > SiO2 > TiO2 and Ag > SiO2 > ZnO > TiO2, respectively. As the synthesized NPs inhibit bacterial growth and suppress the activity of urease and DNA polymerase, the use of these NPs to control pathogens is proposed.
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RefWorks
Reference Manager
BibTeX
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-
1.
Piccinno, F., Gottschalk, F., Seeger, S., Nowack, B.: Industrial production quantities and uses of ten engineered nanomaterials in Europe and the world. J Nanoparticle Res 14, 1–11 (2012).
-
2.
Abeylath, S. C., Turos, E.: Drug delivery approaches to overcome bacterial resistance to beta-lactam antibiotics. Expert Opin Drug Deliv 5, 931–949 (2008).
-
3.
Espitia, P. J. P., Soares, N. D. F. F., dos Reis Coimbra, J. S., de Andrade, N. J., Cruz, R. S., Medeiros, E. A. A.: Zinc oxide nanoparticles: Synthesis, antimicrobial activity and food packaging applications. Food Bioprocess Technol 5, 1447–1464 (2012).
-
4.
Weir, A., Westerhoff, P., Fabricius, L., Hristovski, K., von Goetz, N.: Titanium dioxide nanoparticles in food and personal care products. Environ Sci Technol 46, 2242–2250 (2012).
-
5.
Khan, S. T., Al-Khedhairy, A. A., Musarrat, J.: ZnO and TiO2 nanoparticles as novel antimicrobial agents for oral hygiene: A review. J Nanoparticle Res 17, 1–16 (2015).
-
6.
Khan, S. T., Ahamed, M., Al-Khedhairy, A., Musarrat, J.: Biocidal effect of copper and zinc oxide nanoparticles on human oral microbiome and biofilm formation. Mater Lett 97, 67–70 (2013).
-
7.
Rai, M., Deshmukh, S., Ingle, A., Gade, A.: Silver nanoparticles: The powerful nanoweapon against multidrug-resistant bacteria. J Appl Microbiol 112, 841–852 (2012).
-
8.
Khan, S. T., Musarrat, J., Al-Khedhairy, A. A.: Countering drug resistance, infectious diseases, and sepsis using metal and metal oxides nanoparticles: Current status. Colloids Surf B Biointerfaces 146, 70–83 (2016).
-
9.
Rutherford, J. C.: The emerging role of urease as a general microbial virulence factor. PLoS Pathogens 10, e1004062 (2014).
-
10.
Dai, X. R., Karring, H.: A determination and comparison of urease activity in feces and fresh manure from pig and cattle in relation to ammonia production and pH changes. PLoS One 9, e110402 (2014).
-
11.
Li, K., Zhao, X., Hammer, B. K., Du, S., Chen, Y.: Nanoparticles inhibit DNA replication by binding to DNA: Modeling and experimental validation. ACS Nano 7, 9664–9674 (2013).
-
12.
Paillusson, F., Dahirel, V., Jardat, M., Victor, J.-M., Barbi, M.: Effective interaction between charged nanoparticles and DNA. Phys Chem Chem Phys 13, 12603–12613 (2011).
-
13.
Railsback, J. G., Singh, A., Pearce, R. C., McKnight, T. E., Collazo, R., Sitar, Z., Yingling, Y. G., Melechko, A. V.: Weakly charged cationic nanoparticles induce DNA bending and strand separation. Adv Mater 24, 4261–4265 (2012).
-
14.
Nandanwar, R., Singh, P., Haque, F. Z., Shabanda, I., Kabiru, N., Bolognesi, L. F. C., Borges, F. A., Cinman, J. L. F., da Silva, R. G., dos Santos, A. G.: Synthesis and characterization of SiO2 nanoparticles by sol-gel process and its degradation of methylene blue. Am Chem Sci J 5, 1–10 (2015).
-
15.
Raja, M., Shanmugaraj, A. M., Ryu, S. H.: Preparation of template free zinc oxide nanoparticles using sol-gel chemistry. J Nanosci Nanotechnol 8, 4224–4226 (2008).
-
16.
Castro, A., Nunes, M., Carvalho, A., Costa, F., Florencio, M.: Synthesis of anatase TiO2 nanoparticles with high temperature stability and photocatalytic activity. Solid State Sci 10, 602–606 (2008).
-
17.
Khan, M., Khan, S. T., Adil, S. F., Musarrat, J., Al-Khedhairy, A. A., Al-Warthan, A., Siddiqui, M. R., Alkhathlan, H. Z.: Antibacterial properties of silver nanoparticles synthesized using Pulicaria glutinosa plant extract as a green bioreductant. Int J Nanomed 9, 3551–3565 (2014).
-
18.
Khan, S. T., Nakagawa, Y., Harayama, S.: Galbibacter mesophilus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae. Int J Syst Bacteriol 57, 969–973 (2007).
-
19.
Lundin, C., North, M., Erixon, K., Walters, K., Jenssen, D., Goldman, A. S., Helleday, T.: Methyl methanesulfonate (MMS) produces heat-labile DNA damage but no detectable in vivo DNA double-strand breaks. Nucleic Acids Res 33, 3799–3811 (2005).
-
20.
Wahab, R., Khan, S. T., Dwivedi, S., Ahamed, M., Musarrat, J., Al-Khedhairy, A. A.: Effective inhibition of bacterial respiration and growth by CuO microspheres composed of thin nanosheets. Colloids Surf B Biointerfaces 111, 211–217 (2013).
-
21.
Song, W., Zhang, J., Guo, J., Ding, F., Li, L., Sun, Z.: Role of the dissolved zinc ion and reactive oxygen species in cytotoxicity of ZnO nanoparticles. Toxicol Lett 199, 389–397 (2010).
-
22.
Trouiller, B., Reliene, R., Westbrook, A., Solaimani, P., Schiestl, R. H.: Titanium dioxide nanoparticles induce DNA damage and genetic instability in vivo in mice. Cancer Res 69, 8784–8789 (2009).
-
23.
Kang, T., Guan, R., Chen, X., Song, Y., Jiang, H., Zhao, J.: In vitro toxicity of different-sized ZnO nanoparticles in Caco-2 cells. Nanoscale Res Lett 8, 1–8 (2013).
-
24.
Duan, J., Yu, Y., Li, Y., Yu, Y., Li, Y., Zhou, X., Huang, P., Sun, Z.: Toxic effect of silica nanoparticles on endothelial cells through DNA damage response via Chk1-dependent G2/M checkpoint. PLoS One 8, e62087 (2013).
-
25.
Kim, H. R., Park, Y. J., Da Young Shin, S. M. O., Chung, K. H.: Appropriate in vitro methods for genotoxicity testing of silver nanoparticles. Environ Health Toxicol 28, e2013003 (2013).
-
26.
Pan, X., Redding, J. E., Wiley, P. A., Wen, L., McConnell, J. S., Zhang, B.: Mutagenicity evaluation of metal oxide nanoparticles by the bacterial reverse mutation assay. Chemosphere 79, 113–116 (2010).
-
27.
Ahmad, J., Dwivedi, S., Alarifi, S., Al-Khedhairy, A. A., Musarrat, J.: Use of β-galactosidase (lacZ) gene α-complementation as a novel approach for assessment of titanium oxide nanoparticles induced mutagenesis. Mutat Res 747, 246–252 (2012).
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