View More View Less
  • 1 International University, VNU-HCMC, Vietnam
  • | 2 Aston University, UK
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $784.00

The modulation of efflux pump functions under fluoroquinolone (FQ) exposure is of great concern as it could result in occurrence of multidrug-resistant (MDR) bacterial strains. In this study, MDR mechanism in Pseudomonas aeruginosa induced via moxifloxacin (MOX) pressure was investigated. After serial MOX [concentration of 0.5 × the minimum inhibitory concentration (MIC)] exposure, the fully susceptible P. aeruginosa ATCC 9027 strain has increased its MIC not only toward MOX (1→128 mg/L) but also to other antibiotics. Furthermore, this MOX-exposed strain did not revert to antibiotic-sensitive phenotype when being cultured in antibiotic-free medium for 12 days. No mutation was observed for FQ-target (gyrA and parC) or most investigated efflux regulatory genes (mexT, mexR, and nalC) except nfxB in which a 100-bp deletion was found. This associated with the elevated expression of multidrug efflux pump operon (mexCD-oprJ) which could directly result in MDR phenotype.

  • 1.

    Mesaros, N., Nordmann, P., Plesiat, P., Roussel-Delvallez, M., Van Eldere, J., Glupczynski, Y., Van Laethem, Y., Jacobs, F., Lebecque, P., Malfroot, A., Tulkens, P. M., Van Bambeke, F.: Pseudomonas aeruginosa: Resistance and therapeutic options at the turn of the new millennium. Clin Microbiol Infect 13, 560578 (2007).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 2.

    Strateva, T., Yordanov, D.: Pseudomonas aeruginosa – A phenomenon of bacterial resistance. J Med Microbiol 58, 11331148 (2009).

  • 3.

    Aldred, K. J., McPherson, S. A., Turnbough C. L., Jr. , Kerns, R. J., Osheroff, N.: Topoisomerase IV-quinolone interactions are mediated through a water-metal ion bridge: Mechanistic basis of quinolone resistance. Nucleic Acids Res 41, 46284639 (2013).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 4.

    Gasink, L. B., Fishman, N. O., Weiner, M. G., Nachamkin, I., Bilker, W. B., Lautenbach, E.: Fluoroquinolone-resistant Pseudomonas aeruginosa: Assessment of risk factors and clinical impact. Am J Med 119, 526.e19526.e25 (2006).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 5.

    Redgrave, L. S., Sutton, S. B., Webber, M. A., Piddock, L. J.: Fluoroquinolone resistance: Mechanisms, impact on bacteria, and role in evolutionary success. Trends Microbiol 22, 438445 (2014).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 6.

    Salma, R., Dabboussi, F., Kassaa, I., Khudary, R., Hamze, M.: gyrA and parC mutations in quinolone-resistant clinical isolates of Pseudomonas aeruginosa from Nini Hospital in north Lebanon. J Infect Chemother 19, 7781 (2013).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 7.

    Tian, Z. X., Mac Aogain, M., O’Connor, H. F., Fargier, E., Mooij, M. J., Adams, C., Wang, Y. P., O’Gara, F.: MexT modulates virulence determinants in Pseudomonas aeruginosa independent of the MexEF-OprN efflux pump. Microb Pathog 47, 237241 (2009).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 8.

    Higgins, P. G., Fluit, A. C., Milatovic, D., Verhoef, J., Schmitz, F. J.: Mutations in GyrA, ParC, MexR and NfxB in clinical isolates of Pseudomonas aeruginosa. Int J Antimicrob Agents 21, 409413 (2003).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 9.

    Avrain, L., Garvey, M., Mesaros, N., Glupczynski, Y., Mingeot-Leclercq, M. P., Piddock, L. J., Tulkens, P. M., Vanhoof, R., Van Bambeke, F.: Selection of quinolone resistance in Streptococcus pneumoniae exposed in vitro to subinhibitory drug concentrations. J Antimicrob Chemother 60, 965972 (2007).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 10.

    European Committee on Antimicrobial Susceptibility Testing (EUCAST) documents. Document version 5.0, 2015-05-01.

  • 11.

    Daigle, D. M., Cao, L., Fraud, S., Wilke, M. S., Pacey, A., Klinoski, R., Strynadka, N. C., Dean, C. R., Poole, K.: Protein modulator of multidrug efflux gene expression in Pseudomonas aeruginosa. J Bacteriol 189, 54415451 (2007).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 12.

    Sadeghifard, N., Valizadeh, A., Zolfaghary, M. R., Maleki, M. H., Maleki, A., Mohebi, R., Ghafourian, S., Khosravi, A.: Relationship between the presence of the nalC mutation and multidrug resistance in Pseudomonas aeruginosa. Int J Microbiol 2012, 575193 (2012).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 13.

    Morita, Y., Murata, T., Mima, T., Shiota, S., Kuroda, T., Mizushima, T., Gotoh, N., Nishino, T., Tsuchiya, T.: Induction of mexCD-oprJ operon for a multidrug efflux pump by disinfectants in wild-type Pseudomonas aeruginosa PAO1. J Antimicrob Chemother 51, 991994 (2003).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 14.

    Dumas, J. L., van Delden, C., Perron, K., Kohler, T.: Analysis of antibiotic resistance gene expression in Pseudomonas aeruginosa by quantitative real-time-PCR. FEMS Microbiol Lett 254, 217225 (2006).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 15.

    Jacoby, G. A.: Mechanisms of resistance to quinolones. Clin Infect Dis 41, S120S126 (2005).

  • 16.

    Aeschlimann, J. R.: The role of multidrug efflux pumps in the antibiotic resistance of Pseudomonas aeruginosa and other gram-negative bacteria. Insights from the Society of Infectious Diseases Pharmacists. Pharmacotherapy 23, 916924 (2003).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 17.

    Hooper, D. C.: Emerging mechanisms of fluoroquinolone resistance. Emerg Infect Dis 7, 337341 (2001).

  • 18.

    Jalal, S., Ciofu, O., Hoiby, N., Gotoh, N., Wretlind, B.: Molecular mechanisms of fluoroquinolone resistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients. Antimicrob Agents Chemother 44, 710712 (2000).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 19.

    Henrichfreise, B., Wiegand, I., Pfister, W., Wiedemann, B.: Resistance mechanisms of multiresistant Pseudomonas aeruginosa strains from Germany and correlation with hypermutation. Antimicrob Agents Chemother 51, 40624070 (2007).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 20.

    Poole, K.: Pseudomonas aeruginosa: Resistance to the max. Front Microbiol 2, 65 (2011).

  • 21.

    Monti, M. R., Morero, N. R., Miguel, V., Argarana, C. E.: nfxB as a novel target for analysis of mutation spectra in Pseudomonas aeruginosa. PLoS One 8, e66236 (2013).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 22.

    Purssell, A., Poole, K.: Functional characterization of the NfxB repressor of the mexCD-oprJ multidrug efflux operon of Pseudomonas aeruginosa. Microbiology 159, 20582073 (2013).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 23.

    Hosaka, M., Gotoh, N., Nishino, T.: Purification of a 54-kilodalton protein (OprJ) produced in NfxB mutants of Pseudomonas aeruginosa and production of a monoclonal antibody specific to OprJ. Antimicrob Agents Chemother 39, 17311735 (1995).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 24.

    Jakics, E. B., Iyobe, S., Hirai, K., Fukuda, H., Hashimoto, H.: Occurrence of the nfxB type mutation in clinical isolates of Pseudomonas aeruginosa. Antimicrob Agents Chemother 36, 25622565 (1992).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 25.

    Jeannot, K., Elsen, S., Kohler, T., Attree, I., van Delden, C., Plesiat, P.: Resistance and virulence of Pseudomonas aeruginosa clinical strains overproducing the MexCD-OprJ efflux pump. Antimicrob Agents Chemother 52, 24552462 (2008).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 26.

    Kiser, T. H., Obritsch, M. D., Jung, R., MacLaren, R., Fish, D. N.: Efflux pump contribution to multidrug resistance in clinical isolates of Pseudomonas aeruginosa. Pharmacotherapy 30, 632638 (2010).

    • Crossref
    • Search Google Scholar
    • Export Citation

 

The author instruction is available in PDF.
Please, download the file from HERE

Senior editors

Editor-in-Chief: Prof. Dóra Szabó (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Managing Editor: Dr. Béla Kocsis (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Co-editor: Dr. Andrea Horváth (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Editorial Board

  • Prof. Éva ÁDÁM (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Sebastian AMYES (Department of Medical Microbiology, University of Edinburgh, Edinburgh, UK.)
  • Dr. Katalin BURIÁN (Institute of Clinical Microbiology University of Szeged, Szeged, Hungary; Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.)
  • Dr. Orsolya DOBAY (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Ildikó Rita DUNAY (Institute of Inflammation and Neurodegeneration, Medical Faculty, Otto-von-Guericke University, Magdeburg, Germany; Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany)
  • Prof. Levente EMŐDY(Department of Medical Microbiology and Immunology, University of Pécs, Pécs, Hungary.)
  • Prof. Anna ERDEI (Department of Immunology, Eötvös Loránd University, Budapest, Hungary, MTA-ELTE Immunology Research Group, Eötvös Loránd University, Budapest, Hungary.)
  • Prof. Éva Mária FENYŐ (Division of Medical Microbiology, University of Lund, Lund, Sweden)
  • Prof. László FODOR (Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary)
  • Prof. József KÓNYA (Department of Medical Microbiology, University of Debrecen, Debrecen, Hungary)
  • Prof. Yvette MÁNDI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Károly MÁRIALIGETI (Department of Microbiology, Eötvös Loránd University, Budapest, Hungary)
  • Prof. János MINÁROVITS (Department of Oral Biology and Experimental Dental Research, University of Szeged, Szeged, Hungary)
  • Prof. Béla NAGY (Centre for Agricultural Research, Institute for Veterinary Medical Research, Budapest, Hungary.)
  • Prof. István NÁSZ (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Kristóf NÉKÁM (Hospital of the Hospitaller Brothers in Buda, Budapest, Hungary.)
  • Dr. Eszter OSTORHÁZI (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Rozália PUSZTAI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Peter L. RÁDY (Department of Dermatology, University of Texas, Houston, Texas, USA)
  • Prof. Éva RAJNAVÖLGYI (Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary)
  • Prof. Ferenc ROZGONYI (Institute of Laboratory Medicine, Semmelweis University, Budapest, Hungary)
  • Prof. Zsuzsanna SCHAFF (2nd Department of Pathology, Semmelweis University, Budapest, Hungary)
  • Prof. Joseph G. SINKOVICS (The Cancer Institute, St. Joseph’s Hospital, Tampa, Florida, USA)
  • Prof. Júlia SZEKERES (Department of Medical Biology, University of Pécs, Pécs, Hungary.)
  • Prof. Mária TAKÁCS (National Reference Laboratory for Viral Zoonoses, National Public Health Center, Budapest, Hungary.)
  • Prof. Edit URBÁN (Department of Medical Microbiology and Immunology University of Pécs, Pécs, Hungary; Institute of Translational Medicine, University of Pécs, Pécs, Hungary.)

 

Editorial Office:
Akadémiai Kiadó Zrt.
Budafoki út 187-187, A/3, H-1117 Budapest, Hungary

Editorial Correspondence:
Acta Microbiologica et Immunologica Hungarica
Institute of Medical Microbiology
Semmelweis University
P.O. Box 370
H-1445 Budapest, Hungary
Phone: + 36 1 459 1500 ext. 56101
Fax: (36 1) 210 2959
E-mail: amih@med.semmelweis-univ.hu

 Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • SCOPUS
  • Science Citation Index Expanded
2020  
Total Cites 662
WoS
Journal
Impact Factor
2,048
Rank by Immunology 145/162(Q4)
Impact Factor Microbiology 118/137 (Q4)
Impact Factor 1,904
without
Journal Self Cites
5 Year 0,671
Impact Factor
Journal  0,38
Citation Indicator  
Rank by Journal  Immunology 146/174 (Q4)
Citation Indicator  Microbiology 120/142 (Q4)
Citable 42
Items
Total 40
Articles
Total 2
Reviews
Scimago 28
H-index
Scimago 0,439
Journal Rank
Scimago Immunology and Microbiology (miscellaneous) Q4
Quartile Score Medicine (miscellaneous) Q3
Scopus 438/167=2,6
Scite Score  
Scopus General Immunology and Microbiology 31/45 (Q3)
Scite Score Rank  
Scopus 0,760
SNIP
Days from  225
sumbission
to acceptance
Days from  118
acceptance
to publication
Acceptance 19%
Rate

2019  
Total Cites
WoS
485
Impact Factor 1,086
Impact Factor
without
Journal Self Cites
0,864
5 Year
Impact Factor
1,233
Immediacy
Index
0,286
Citable
Items
42
Total
Articles
40
Total
Reviews
2
Cited
Half-Life
5,8
Citing
Half-Life
7,7
Eigenfactor
Score
0,00059
Article Influence
Score
0,246
% Articles
in
Citable Items
95,24
Normalized
Eigenfactor
0,07317
Average
IF
Percentile
7,690
Scimago
H-index
27
Scimago
Journal Rank
0,352
Scopus
Scite Score
320/161=2
Scopus
Scite Score Rank
General Immunology and Microbiology 35/45 (Q4)
Scopus
SNIP
0,492
Acceptance
Rate
16%

 

Acta Microbiologica et Immunologica Hungarica
Publication Model Online only Hybrid
Submission Fee none
Article Processing Charge 1100 EUR/article
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Online subsscription: 652 EUR / 812 USD
Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content.
Purchase per Title Individual articles are sold on the displayed price.

Acta Microbiologica et Immunologica Hungarica
Language English
Size A4
Year of
Foundation
1954
Publication
Programme
2021 Volume 68
Volumes
per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 1217-8950 (Print)
ISSN 1588-2640 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Feb 2021 12 0 0
Mar 2021 2 0 0
Apr 2021 5 0 0
May 2021 20 0 0
Jun 2021 14 0 0
Jul 2021 5 0 0
Aug 2021 0 0 0