Enterococci are opportunistic bacteria that cause severe infections in animals and humans, capable to acquire, express, and transfer antimicrobial resistance. Susceptibility to 21 antimicrobial agents was tested by the disk diffusion method in 222 Enterococcus spp. strains isolated from the fecal samples of 287 healthy domestic dogs. Vancomycin and ampicillin minimum inhibitory concentrations (MICs) and high-level aminoglycoside resistance (HLAR) tests were also performed. Isolates showed resistance mainly to streptomycin (88.7%), neomycin (80.6%), and tetracycline (69.4%). Forty-two (18.9%) isolates showed an HLAR to streptomycin and 15 (6.7%) to gentamicin. Vancomycin and ampicillin MIC values showed 1 and 18 resistant strains, respectively. One hundred and thirty-six (61.2%) strains were classified as multidrug resistant and six (2.7%) strains as possibly extensively drug-resistant bacteria. Enterococcus faecium and Enterococcus faecalis were the most prevalent antimicrobial resistant species. Companion animals, which often live in close contact with their owners and share the same environment, represent a serious source of enterococci resistant to several antibiotics; for this reason, they may be a hazard for public health by providing a conduit for the entrance of resistance genes into the community.
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-
1.
Byappanahalli, M. N., Nevers, M. B., Korajkic, A., Staley, Z. R., Harwood, V. J.: Enterococci in the environment. Microbiol Mol Bio Rev 4, 685–706 (2012).
-
2.
Franz, C. M., Huch, M., Abriouel, H., Holzapfel, W., Galvez, A.: Enterococci as probiotics and their implications in food safety. Int J Food Microbiol 151, 125–140 (2011).
-
3.
Herczegh, A., Gyurkovics, M., Ghidan, A., Megyesi, M., Lohinai, Z.: Effect of dentin powder on the antimicrobial properties of hyperpure chlorine-dioxide and its comparison to conventional endodontic disinfecting agents. Acta Microbiol Immunol Hung 61, 209–220 (2014).
-
4.
Robbins, K. M., Suyemoto, M. M., Lyman, R. L., Martin, M. P., Barnes, H. J., Borst, L. B.: An outbreak and source investigation of enterococcal spondylitis in broiler caused by Enterococcus cecorum. Avian Dis 56, 768–773 (2012).
-
5.
Šeputienė, V., Bogdaitė, A., Ružauskas, M., Sužiedėlienė, E.: Antibiotic resistance genes and virulence factors in Enterococcus faecium and Enterococcus faecalis from diseased farm animals: Pigs, cattle and poultry. Pol J Vet Sci 3, 431–438 (2012).
-
6.
Abbot, Y., Kirby, B. M., Karczmarczk, M., Markey, B. K., Leonard, F. C., Fitzgerald, S.: High-level gentamicin-resistant and vancomycin-resistant Enterococcus faecium isolated from a wound in a dog. J Small Anim Pract 50, 194–197 (2009).
-
7.
Semedo-Lemsaddek, T., Tavares, M., São Braz, B., Tavares, L., Oliveira, M.: Enterococcal infective endocarditis following periodontal disease in dogs. PLoS One 11, e0146860 (2016).
-
8.
Tendolkar, P. M., Baghdayan, A. S., Shankar, N.: Pathogenic enterococci: New developments in the 21st century. Cell Mol Life Sci 60, 2622–2636 (2003).
-
9.
Hegstad, K., Mikalsenm, T., Coque, T. M., Werner, G., Sundsfjord, A.: Mobile genetics elements and their contribution to the emergence of antimicrobial resistant Enterococcus faecalis and Enterococcus faecium. Clin Microbiol Infect 16, 541–554 (2010).
-
10.
Bauer, A., Kirby, W. M. N., Sherris, J. C., Turk, M.: Antibiotic susceptibility testing by a standardized single disc method. Am J Pathol 45, 493–496 (1966).
-
11.
Clinical and Laboratory Standards Institute (CLSI): Performance standards for antimicrobial susceptibility testing, seventeenth informational supplement. Clinical and Laboratory Standards Institute document M100-S17, Clinical and Laboratory Standards Institute, Wayne, PA, USA, 2007.
-
12.
The European Committee on Antimicrobial Susceptibility Testing (EUCAST). Breakpoint tables for interpretation of MICs and zone diameters. Version 3.1, 2013. Retrieved from http://www.eucast.org
-
13.
Clinical and Laboratory Standards Institute (CLSI). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard, Seventh edition. Clinical and Laboratory Standards Institute document M7-A7. Clinical and Laboratory Standards Institute, Wayne, PA, USA, 2006.
-
14.
Magiorakos, A. P., Srinivasan, A., Carey, R. B., Carmeli, Y., Falagas, M. E., Giske, C. G., Harbarth, S., Hindler, J. F., Kahlmeter, G., Olsson-Liljequist, B., Paterson, D. L., Rice, L. B., Stelling, J., Struelens, M. J., Vatopoulos, A., Weber, J. T., Monnet, D. L.: Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect 18, 268–281 (2012).
-
15.
Devriese, L. A., Cruz Colque, J. I., De Herdt, P., Haesebrouck, F.: Identification and composition of the tonsillar and anal enterococcal and streptococcal flora of dogs and cats. J Appl Bacteriol 73, 421–425 (1992).
-
16.
Ossiprandi, M. C., Bottarelli, E., Cattabiani, F., Bianchi, E.: Susceptibility to vancomycin and other antibiotics of 165 Enterococcus strains isolated from dogs in Italy. Comp Immunol Microbiol Infect Dis 31, 1–9 (2008).
-
17.
Iseppi, R., Messi, P., Anacarso, I., Bondi, M., Sabia, C., Condò, C., de Niederhausern, S.: Antimicrobial resistance and virulence traits in Enterococcus strains isolated from dogs and cats. New Microbiol 38, 369–378 (2015).
-
18.
Hollenbeck, B. L., Rice, L. B.: Intrinsic and acquired resistance mechanisms in Enterococcus. Virulence 5, 421–433 (2012).
-
19.
Guardabassi, L., Schwarz, S., Lloyd, D. H.: Pet animals as reservoirs of antimicrobial-resistant bacteria. J Antimicrob Chemother 54, 321–332 (2004).
-
20.
Sun, Y., Cai, Y., Liu, X., Bai, N., Liang, B., Wang, R.: The emergence of clinical resistance to tigecycline. Int J Antimicrob Agents 41, 110–116 (2013).
-
21.
Redgrave, L. S., Sutton, S. B., Webber, M. A., Piddock, L. J. V.: Fluoroquinolone resistance: Mechanism, impact on bacteria, and role in evolutionary success. Trends Microbiol 22, 438–445 (2014).
-
22.
Jaglic, Z., Vlkova, H., Bardon, J., Michu, E., Cervinkova, D., Babak, V.: Distribution, characterization and genetic bases of erythromycin resistance in staphylococci and enterococci originating from livestock. Zoonoses Public Health 59, 202–211 (2012).
-
23.
Adhikari, L.: High-level aminoglycoside resistance and reduced susceptibility to vancomycin in nosocomial enterococci. J Glob Infect Dis 2, 231–235 (2010).
-
24.
Padmasini, E., Padmaraj, R., Srivani Ramesh, S.: High level aminoglycoside resistance and distribution of aminoglycoside resistant genes among clinical isolates of Enterococcus species in Chennai, India. Sci World J 2014, 329157 (2014).
-
25.
Jackson, C. R., Fedorka-Cray, P. J., Davis, J. A., Barrett, J. B., Brousse, J. H., Gustafson, J., Kucher, M.: Mechanisms of antimicrobial resistance and genetic relatedness among enterococci isolated from dogs and cats in the United States. J Appl Microbiol 108, 2171–2179 (2010).
-
26.
Jovanović, M., Milošević, B., Tošić, T., Stevanović, G., Mioljević, V., Indić, N., Velebit, B., Zervos, M.: Molecular typing, pathogenicity factor genes and antimicrobial susceptibility of vancomycin resistant enterococci in Belgrade, Serbia. Acta Microbiol Immunol Hung 62, 147–160 (2015).
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