Karst areas have great environmental importance as sources of subsurface water and often maintain very sensitive ecosystems. In recent years, increasing number of microbiological studies focused on the bacterial communities of karst soils. In this study, diversity examinations on two distinct Hungarian karst areas, Aggtelek and Tapolca, were performed using parallel cultivation and molecular cloning methods. The phylogenetic affiliation of bacterial strains and molecular clones was determined based on their 16S rRNA gene sequences. Bacterial isolates were identified as members of the phyla Actinobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. Besides the taxa identified by cultivation, members of the phyla Chloroflexi, Cyanobacteria, Acidobacteria, Verrucomicrobia, and Gemmatimonadetes were detected by the cloning. The difference in the composition of soil bacterial communities was related to geographic locations and soil types. Both the highest and the lowest bacterial diversities were detected in samples from Aggtelek National Park, characterized by Leptic Luvisol and Rendzic Leptosol soil types. The difference in the composition of bacterial communities between Rendzic Leptosol and Leptic Phaeozem soil types at Tapolca could be the result of human impacts.
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-
1.
Keveiné-Bárány, I.: Genetic types, human impacts, and protection of Hungarian karsts. Acta Climatologica Chorologica 38–39, 17–23 (2005).
-
2.
Zhang, P., Li, L., Pan, G., Ren, J.: Soil quality changes in land degradation as indicated by soil chemical, biochemical and microbiological properties in a karst area of southwest Guizhou, China. Environ Geol 51, 609–619 (2006).
-
3.
Zhou, J. P., Huang, Y., Mo, M. H.: Phylogenetic analysis on the soil bacteria distributed in karst forest. Braz J Microbiol 40, 827–837 (2009).
-
4.
Zhu, H., He, X., Wang, K., Su, Y., Wu, J.: Interactions of vegetation succession, soil bio-chemical properties and microbial communities in a karst ecosystem. Eur J Soil Biol 51, 1–7 (2012).
-
5.
Chen, X., Su, Y., He, X., Wei, Y., Wei, W., Wu, J.: Soil bacterial community composition and diversity respond to cultivation in karst ecosystems. World J Microbiol Biotechnol 28, 205–213 (2012).
-
6.
Hu, N., Li, H., Tang, Z., Li, Z., Li, G., Jiang, Y., Hu, X., Lou, Y.: Community size, activity and C:N stoichiometry of soil microorganisms following reforestation in a karst region. Eur J Soil Biol 73, 77–83 (2016).
-
7.
Zhang, X., Liu, S., Li, X., Wang, J., Ding, Q., Wang, H., Tian, C., Yao, M., An, J., Huang, Y.: Changes of soil prokaryotic communities after clear-cutting in a karst forest: Evidences for cutting-based disturbance promoting deterministic processes. FEMS Microbiol Ecol 92, fiw026 (2016).
-
8.
Zámbó, L.: The experimental examination of microbial origin corrosion aggressivity of karst soils. Acta Carsologica 16, 261–275 (1998).
-
9.
Knáb, M., Szili-Kovács, T., Kiss, K., Palatinszky, M., Márialigeti, K., Móga, J., Borsodi, A. K.: Comparison of soil microbial communities from two distinct karst areas in Hungary. Acta Microbiol Immunol Hung 59, 91–105 (2012).
-
10.
Janssen, P. H., Yates, P. S., Grinton, B. E., Taylor, P. M., Sait, M.: Improved culturability of soil bacteria and isolation in pure culture of novel members of the divisions Acidobacteria, Actinobacteria, Proteobacteria, and Verrucomicrobia. Appl Environ Microbiol 68, 2391–2396 (2002).
-
11.
Cavaglieri, L., Orlando, J., Etcheverry, M.: Rhizosphere microbial community structure at different maize plant growth stages and root locations. Microbiol Res 164, 391–399 (2009).
-
12.
Kumar, G., Kanaujia, N., Bafan, A.: Functional and phylogenetic diversity of root-associated bacteria of Ajuga bracteosa in Kangra valley. Microbiol Res 167, 220–225 (2012).
-
13.
Shade, A., Hogan, C. S., Klimowicz, A. K., Linske, M., McManus, P. S., Handelsman, J.: Culturing captures members of the soil rare biosphere. Environ Microbiol 14, 2247–2252 (2012).
-
14.
Quirino, B. F., Pappas, G. J., Tagliaferro, A. C., Collevatti, R. G., Neto, E. L., da Silva, M. R. S. S., Bustamante, M. M. C., Krüger, R. H.: Molecular phylogenetic diversity of bacteria associated with soil of the savanna-like Cerrado vegetation. Microbiol Res 164, 59–70 (2009).
-
15.
Baldrian, P., Kolařík, M., Štursová, M., Kopecký, J., Valášková, V., Větrovský, T., Žifčáková, L., Šnajdr, J., Rídl, J., Vlček, C., Voříšková, J.: Active and total microbial communities in forest soil are largely different and highly stratified during decomposition. ISME J 6, 248–258 (2012).
-
16.
Hartmann, M., Howes, C. G., Van Insberghe, D., Yu, H., Bachar, D., Christen, R., Nilsson, R.H., Hallam, S.J., Mohn, W.W. Significant and persistent impact of timber harvesting on soil microbial communities in northern coniferous forests. ISME J 6, 2199–2218 (2012).
-
17.
Dunbar, J., Takala, S., Barns, S. M., Davis, J. A., Kuske, C. R.: Levels of bacterial community diversity in four arid soils compared by cultivation and 16S rRNA gene cloning. Appl Environ Microbiol 65, 1662–1669 (1999).
-
18.
Felske, A., Wolterink, A., van Lis, R., de Vos, W. M., Akkermans, A. D.: Searching for predominant soil bacteria: 16S rDNA cloning versus strain cultivation. FEMS Microb Ecol 30, 137–145 (1999).
-
19.
Ramírez-Saad, H. C., Sessitsch, A., Akkermans, A. D. L.: Molecular diversity in the bacterial community and the fluorescent pseudomonads group in natural and chlorobenzoate-stressed peat-forest soil. Microbiol Res 158, 47–54 (2003).
-
20.
Van Insberghe, D., Hartmann, M., Stewart, G. R., Mohn, W. W.: Isolation of a substantial proportion of forest soil bacterial communities detected via pyrotag sequencing. Appl Environ Microbiol 79, 2096–2098 (2013).
-
21.
Waksman, S. A.: The Actinomycetes I-II. Williams & Wilkins Co., Baltimore, 1961.
-
22.
Lane, D. J.: 16S/23S rRNA sequencing. In Stackebrandt, E., Goodfellow, M. (eds): Nucleic Acid Techniques in Bacterial Systematics. Wiley, New York, 1991, pp. 115–175.
-
23.
Nübel, U., Engelen, B., Felske, A., Snaidr, J., Wieshuber, A., Amann, R. I., Ludwig, W., Backhaus, H.: Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. J Bacteriol 178, 5636–5643 (1996).
-
24.
Sambrook, J., Russell, D. W.: Molecular Cloning. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 2001.
-
25.
Massol-Deya, A. A., Odelson, D. A., Hickey, R. F., Tiedje, J. M.: Bacterial community fingerprinting of amplified 16S and 16S-23S ribosomal DNA gene sequences and restriction endonuclease analysis (ARDRA). In Akkermans, A. D. L., Van Elsas, J. D., De Bruijn, F. J. (eds): Molecular Microbial Ecology Manual 3.3.2, 1995, pp 289–296.
-
26.
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., Lipman, D. J.: Basic local alignment search tool. J Mol Biol 215, 403–410 (1990).
-
27.
Kim, O. S., Cho, Y. J., Lee, K., Yoon, S. H., Kim, M., Na, H., Park, S. C., Jeon, Y. S., Lee, J. H., Yi, H., Won, S., Chun, J.: Introducing EzTaxon: A prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62, 716–721 (2012).
-
28.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S.: MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28, 2731–2739 (2011).
-
29.
Shannon, C. E., Weaver, W.: The Mathematical Theory of Communication. University of Illinois Press, Urbana, 1949.
-
30.
Hammer, R., Harper, D. A. T., Ryan, P. D.: PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4, 9 (2001).
-
31.
Ward, N. L., Challacombe, J. F., Janssen, P. H., Henrissat, B., Coutinho, P. M., Wu, M., Xie, G., Haft, D. H., Sait, M., Badger, J., Barabote, R. D., Bradley, B., Brettin, T. S., Brinkac, L. M., Bruce, D., Creasy, T., Daugherty, S. C., Davidsen, T. M., DeBoy, R. T., Detter, J. C., Dodson, R. J., Durkin, A. S., Ganapathy, A., Gwinn-Giglio, M., Han, C. S., Khouri, H., Kiss, H., Kothari, S. P., Madupu, R., Nelson, K. E., Nelson, W. C., Paulsen, I., Penn, K., Ren, Q., Rosovitz, M. J., Selengut, J. D., Shrivastava, S., Sullivan, S. A., Tapia, R., Thompson, L. S., Watkins, K. L., Yang, Q., Yu, C., Zafar, N., Zhou, L., Kuske, C. R.: Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils. Appl Environ Microbiol 75, 2046–2056 (2009).
-
32.
Araujo, J. F., de Castro, A. P., Costa, M. M. C., Togawa, R. C., Júnior, G. J. P., Quirino, B. F., Bustamante, M. M. C., Williamson, L., Handelsman, J., Krüger, R. H.: Characterization of soil bacterial assemblies in Brazilian savanna-like vegetation reveals Acidobacteria dominance. Microb Ecol 64, 760–770 (2012).
-
33.
Bachar, A., Soares, M. I. M., Gillor, O.: The effect of resource islands on abundance and diversity of bacteria in arid soils. Microb Ecol 63, 694–700 (2012).
-
34.
Lladó, S., Žifčáková, L., Větrovský, T., Eichlerová, I., Baldrian, P.: Functional screening of abundant bacteria from acidic forest soil indicates the metabolic potential of Acidobacteria subdivision 1 for polysaccharide decomposition. Biol Fertil Soils 52, 251–260 (2016).
-
35.
Elshahed, M. S., Youssef, N. H., Spain, A. M., Sheik, C., Najar, F. Z., Sukharnikov, L. O., Roe, B. A., Davis, J. P., Schloss, P. D., Bailey, V. L., Krumholz, L. R.: Novelty and uniqueness patterns of rare members of the soil biosphere. Appl Environ Microbiol 74, 5422–5428 (2008).
-
36.
Upchurch, R., Chiu, C. Y., Everett, K., Dyszynski, G., Coleman, D. C., Whitman, W. B.: Differences in the composition and diversity of bacterial communities from agricultural and forest soils. Soil Biol Biochem 40, 1294–1305 (2008).
-
37.
Jangid, K., Williams, M. A., Franzluebbers, A. J., Blair, J. M., Coleman, D. C., Whitman, W. B.: Development of soil microbial communities during tallgrass prairie restoration. Soil Biol Biochem 42, 302–312 (2012).
-
38.
Jangid, K., Williams, M. A., Franzluebbers, A. J., Schmidt, T. M., Coleman, D. C., Whitman, W. B.: Land-use history has a stronger impact on soil microbial community composition than aboveground vegetation and soil properties. Soil Biol Biochem 43, 2184–2193 (2011).
-
39.
Macalady, J. L., Jones, D. S., Lyon, E. H.: Extremely acidic, pendulous cave wall biofilms from the Frasassi cave system, Italy. Environ Microbiol 9, 1402–1414 (2007).
-
40.
Alexandre, A., Brigido, C., Laranjo, M., Rodrigues, S., Oliveira, S.: Survey of Chickpea Rhizobia diversity in Portugal reveals the predominance of species distinct from Mesorhizobium ciceri and Mesorhizobium mediterraneum. Microb Ecol 58, 930–941 (2009).
-
41.
Militon, C., Boucher, D., Vachelard, C., Perchet, G., Barra, V., Troquet, J., Peyretaillade, E., Peyret, P.: Bacterial community changes during bioremediation of aliphatic hydrocarbon-contaminated soil. FEMS Microbiol Ecol 74, 669–681 (2010).
-
42.
Wust, P. K., Horn, M. A., Drake, H. L.: Clostridiaceae and Enterobacteriaceae as active fermenters in earthworm gut content. ISME J 5, 92–106 (2011).
-
43.
Martin-Laurent, F., Phillipot, L., Hallet, S., Chaussod, R., Germon, J. C., Soulas, G., Catroux, G.: DNA extraction from soils: Old bias for new microbial diversity analysis methods. Appl Environ Microbiol 67, 2354–2359 (2001).
-
44.
Elsas, J. D., Jansson, J. K., Trevors, J. T.: Modern Soil Microbiology, 2 nd E d ition. CRC Press/Taylor & Francis, Boca Raton, FL, 2007.
-
45.
Lauro, M. F., McDougald, D., Thomas, T., Williams, T. J., Egan, S., Rice, S., DeMaere, M. Z.: The genomic basis of trophic strategy in marine bacteria. PNAS 106, 15527–15533 (2009).
-
46.
Jakobi, K., Hertweck, C.: A gene cluster encoding resistomycin biosynthesis in Streptomyces resistomycificus; Exploring polyketide cyclization beyond linear and angucyclic patterns. J Am Chem Soc 126, 2298–2299 (2004).
-
47.
Ueberschaar, N., Le Sage Tchize Ndejouong, B., Ding, L., Maier, A., Fiebig, H. H., Hertweck, C.: Hydrazidomycins, cytotoxic alkylhydrazides from Streptomyces atratus. Bioorg Med Chem Lett 21, 5839–5841 (2011).
-
48.
Li, H., Lin, Y., Guan, W., Chang, J., Xu, L., Guo, J., Wei, G.: Biosorption of Zn (II) by live and dead cells of Streptomyces ciscaucasicus strain CCNWHX 72-14. J Hazard Mater 179, 151–159 (2010).
-
49.
Guo, J. K., Lin, Y. B., Zhao, M. L., Sun, R., Wang, T. T., Tang, M., Wei, G. H.: Streptomyces plumbiresistens sp. nov., a lead-resistant actinomycete isolated from lead-polluted soil in north-west China. Int J Syst Evol Microbiol 59, 1326–1330 (2009).
-
50.
Xu, J. L., He, J., Wang, Z. C., Wang, K., Li, W. J., Tang, S. K., Li, S. P.: Rhodococcus qingshengii sp. nov., a carbendazim-degrading bacterium. Int J Syst Evol Microbiol 57, 2754–2757 (2007).
-
51.
Groth, I., Vettermann, R., Schuetze, B., Schumann, P., Saiz-Jimenez, C.: Actinomycetes in Karstic caves of northern Spain (Altamira and Tito Bustillo). J Microb Methods 36, 115–122 (1999).
-
52.
Jurado, V., Groth, I., Gonzalez, J. M., Laiz, L., Saiz-Jimenez, C.: Agromyces salentinus sp. nov. and Agromyces neolithicus sp. nov. Int J Syst Evol Microbiol 55, 153–157 (2005).
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