View More View Less
  • 1 Artemis Hospitals, India
  • | 2 Artemis Hospitals, India
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $784.00

The aim of the study was to find out the carbapenem resistance rate and prevalence of different carbapenemase genes in Klebsiella pneumoniae and Escherichia coli from a North Indian corporate hospital that receives both Indian and international patients. A total of 528 clinical isolates of E. coli and K. pneumoniae were included in the study. All isolates that were found resistant to carbapenems by MIC testing (Vitek II Compact®) were screened for NDM, OXA-48, VIM, and KPC genes by PCR. Sequencing of NDM gene and transmissibility by conjugation assay were checked on 22 randomly selected NDM-positive isolates. One hundred and fifty-six isolates (29.54%) were carbapenem-resistant. The rate of carbapenem resistance was significantly higher in K. pneumoniae as compared to E. coli (53.9% vs. 15.6%; p < 0.05). The NDM gene was found in 34.6% (54/156), OXA-48 in 31.4% (49/156), co-expression of NDM + OXA-48 in 15.3% (24/156) of the carbapenem-resistant isolates. VIM and KPC were absent in all isolates. NDM gene was significantly more prevalent in E. coli than K. pneumoniae (p < 0.05). All the tested isolates formed transconjugants and NDM-5 was the most common variant in both species (15/22). The presence of plasmid-based NDM calls for stricter surveillance measures in our hospital settings.

  • 1.

    Jacoby, G. A., Munoz-Price, L. S.: The new beta-lactamases. N Engl J Med 352, 380391 (2005).

  • 2.

    van Duin, D., Doi, Y.: The global epidemiology of carbapenemase-producing Enterobacteriaceae. Virulence 8, 460469 (2017).

  • 3.

    Gupta, N., Limbago, B. M., Patel, J. B., Kallen, A. J.: Carbapenem-resistant Enterobacteriaceae: Epidemiology and prevention. Clin Infect Dis 53, 6067 (2011).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 4.

    Falagas, M. E., Tansarli, G. S., Karageorgopoulos, D. E., Vardakas, K. Z.: Deaths attributable to carbapenem-resistant Enterobacteriaceae infections. Emerg Infect Dis 20, 11701175 (2014).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 5.

    Yong, D., Toleman, M. A., Giske, C. G., Cho, H. S., Sundman, K., Lee, K., Walsh, T. R.: Characterization of a new metallo-lactamase gene, blaNDM-1, and a novel erythromycinesterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob Agents Chemother 3, 504654 (2009).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 6.

    Shrestha, B., Tada, T., Shimada, K., Shrestha, S., Ohara, H., Pokhrel, B. M.: Emergence of various NDM-type-metallo-β-lactamase-producing Escherichia coli clinical isolates in Nepal. Antimicrob Agents Chemother 61, 01425-17 (2017).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 7.

    Rahman, M., Shukla, S.K., Prasad, K. N., Ovejero, C. M., Pati, B. K., Tripathi, A., Singh, A., Srivastava, A. K., Gonzalez-Zorn, B.: Prevalence and molecular characterisation of New Delhi metallo-lactamases NDM-1, NDM-5, NDM-6 and NDM-7 in multidrug-resistant Enterobacteriaceae from India. Int J Antimicrob Agents 44, 3037 (2014).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 8.

    Mohanty, S., Gajanand, M., Gaind, R.: Identification of carbapenemase-mediated resistance among Enterobacteriaceae bloodstream isolates: A molecular study from India. Indian J Med Microbiol 35, 421425 (2017).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 9.

    Walsh, T. R., Weeks, J., Livermore, D. M., Toleman, M. A.: Dissemination of NDM-1 positive bacteria in the New Delhi environment and its implications for human health: An environmental point prevalence study. Lancet Infect Dis 11, 35562 (2011).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 10.

    Veeraraghavan, B., Jesudason, M. R., Prakasah, J. A. J., Anandan, S., Sahni, R. D., Pragasam, A. K., Bakthavatchalam, Y. D., Selvakumar, R. J., Dhole, T. N., Rodrigues, C., Roy, I., Joshi, I. S., Chaudhuri, B. N., Chitnis, D. S.: Antimicrobial susceptibility profiles of gram-negative bacteria causing infections collected across India during 2014–2016: Study for monitoring antimicrobial resistance trend report. Indian J Med Microbiol 36, 3236 (2018).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 11.

    Collee, J. G., Miles, R. S., Wan, B.: Tests for the identification of bacteria. In Collee, J. G., Fraser, A. G., Marmion, B. P., Simmons, A. (eds): Mackie and McCartney Practical Medical Microbiology, 14th Edition. Churchill Livingstone, Edinburgh, 1996, pp. 13150.

    • Search Google Scholar
    • Export Citation
  • 12.

    Clinical and Laboratory standards Institute [CLSI]: Performance Standards for Antimicrobial Susceptibility Testing, 28th Informational Supplement M100. CLSI, Wayne, PA, 2006.

    • Search Google Scholar
    • Export Citation
  • 13.

    Dashti, A. A., Jadaon, M. M., Abdulsamad, A. M., Dashti, H. M.: Heat treatment of bacteria: A simple method of DNA extraction for molecular techniques. Kuwait Med J 41, 117122 (2009).

    • Search Google Scholar
    • Export Citation
  • 14.

    Manchanda, V., Rai, S., Gupta, S., Rautela, R. S., Chopra, R., Rawat, D. S., Verma, V. N., Singh, N. P., Kaur, I. R., Bhalla, P.: Development of TaqMan real-time polymerase chain reaction for the detection of the newly emerging form of carbapenem resistance gene in clinical isolates of Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii. Indian J Med Microbiol 29, 24953 (2011).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 15.

    Dallenne, C., Da Costa, A., Decré, D., Favier, C., Guillaume, A.: Development of a set of multiplex PCR assays for the detection of genes encoding important β-lactamases in Enterobacteriaceae. J Antimicrob Chemother 65, 490495 (2010).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 16.

    Patel, I., Hussain, R., Khan, A., Ahmad, A., Khan, M. U., Hassalai, M. A. A.: Antimicrobial resistance in India. J Pharmaceut Policy Pract 10, 27 (2017).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 17.

    Hussein, N. H.: Emergence of NDM-1 among carbapenem-resistant Klebsiella pneumoniae in Iraqi hospitals. Acta Microbiol Immunol Hung 65, 211227 (2018).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 18.

    Al-Charrakh, A. H., Al-Awadi, S. J., Mohammed, A. S.: Detection of metallo-β-lactamase producing Pseudomonas aeruginosa isolated from public and private hospitals in Baghdad, Iraq. Acta Medica Iranica 54, 107113 (2016).

    • Search Google Scholar
    • Export Citation
  • 19.

    Sharma, A., Bakthavatchalam, Y. D., Gopi, R., Anandan, S., Verghese, V. P., Veeraraghavan, B.: Mechanisms of Carbapenem resistance in K. pneumoniae and E. coli from bloodstream infections in India. J Infect Dis Ther 4, 4 (2016).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 20.

    Grover, S. S., Gupta, N., Gandhoke, I., Batra, J., Hans, C., Khare, S.: New Delhi metallo-β-lactamase-type carbapenemases producing Escherichia coli isolates from hospitalized patients: A pilot study. Indian J Med Res 146, 105110 (2017).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 21.

    Ahmad, N., Ali, S. M., Khan, A. U.: Detection of New Delhi metallo-β-lactamase variants NDM-4, NDM-5, and NDM-7 in Enterobacter aerogenes isolated from a neonatal intensive care unit of a North India hospital: A first report. Microb Drug Resist 24, 161165 (2018).

    • Crossref
    • Search Google Scholar
    • Export Citation

 

The author instruction is available in PDF.
Please, download the file from HERE

Senior editors

Editor-in-Chief: Prof. Dóra Szabó (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Managing Editor: Dr. Béla Kocsis (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Co-editor: Dr. Andrea Horváth (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Editorial Board

  • Prof. Éva ÁDÁM (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Sebastian AMYES (Department of Medical Microbiology, University of Edinburgh, Edinburgh, UK.)
  • Dr. Katalin BURIÁN (Institute of Clinical Microbiology University of Szeged, Szeged, Hungary; Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.)
  • Dr. Orsolya DOBAY (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Ildikó Rita DUNAY (Institute of Inflammation and Neurodegeneration, Medical Faculty, Otto-von-Guericke University, Magdeburg, Germany; Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany)
  • Prof. Levente EMŐDY(Department of Medical Microbiology and Immunology, University of Pécs, Pécs, Hungary.)
  • Prof. Anna ERDEI (Department of Immunology, Eötvös Loránd University, Budapest, Hungary, MTA-ELTE Immunology Research Group, Eötvös Loránd University, Budapest, Hungary.)
  • Prof. Éva Mária FENYŐ (Division of Medical Microbiology, University of Lund, Lund, Sweden)
  • Prof. László FODOR (Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary)
  • Prof. József KÓNYA (Department of Medical Microbiology, University of Debrecen, Debrecen, Hungary)
  • Prof. Yvette MÁNDI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Károly MÁRIALIGETI (Department of Microbiology, Eötvös Loránd University, Budapest, Hungary)
  • Prof. János MINÁROVITS (Department of Oral Biology and Experimental Dental Research, University of Szeged, Szeged, Hungary)
  • Prof. Béla NAGY (Centre for Agricultural Research, Institute for Veterinary Medical Research, Budapest, Hungary.)
  • Prof. István NÁSZ (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Kristóf NÉKÁM (Hospital of the Hospitaller Brothers in Buda, Budapest, Hungary.)
  • Dr. Eszter OSTORHÁZI (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Rozália PUSZTAI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Peter L. RÁDY (Department of Dermatology, University of Texas, Houston, Texas, USA)
  • Prof. Éva RAJNAVÖLGYI (Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary)
  • Prof. Ferenc ROZGONYI (Institute of Laboratory Medicine, Semmelweis University, Budapest, Hungary)
  • Prof. Zsuzsanna SCHAFF (2nd Department of Pathology, Semmelweis University, Budapest, Hungary)
  • Prof. Joseph G. SINKOVICS (The Cancer Institute, St. Joseph’s Hospital, Tampa, Florida, USA)
  • Prof. Júlia SZEKERES (Department of Medical Biology, University of Pécs, Pécs, Hungary.)
  • Prof. Mária TAKÁCS (National Reference Laboratory for Viral Zoonoses, National Public Health Center, Budapest, Hungary.)
  • Prof. Edit URBÁN (Department of Medical Microbiology and Immunology University of Pécs, Pécs, Hungary; Institute of Translational Medicine, University of Pécs, Pécs, Hungary.)

 

Editorial Office:
Akadémiai Kiadó Zrt.
Budafoki út 187-187, A/3, H-1117 Budapest, Hungary

Editorial Correspondence:
Acta Microbiologica et Immunologica Hungarica
Institute of Medical Microbiology
Semmelweis University
P.O. Box 370
H-1445 Budapest, Hungary
Phone: + 36 1 459 1500 ext. 56101
Fax: (36 1) 210 2959
E-mail: amih@med.semmelweis-univ.hu

 Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • SCOPUS
  • Science Citation Index Expanded
2020  
Total Cites 662
WoS
Journal
Impact Factor
2,048
Rank by Immunology 145/162(Q4)
Impact Factor Microbiology 118/137 (Q4)
Impact Factor 1,904
without
Journal Self Cites
5 Year 0,671
Impact Factor
Journal  0,38
Citation Indicator  
Rank by Journal  Immunology 146/174 (Q4)
Citation Indicator  Microbiology 120/142 (Q4)
Citable 42
Items
Total 40
Articles
Total 2
Reviews
Scimago 28
H-index
Scimago 0,439
Journal Rank
Scimago Immunology and Microbiology (miscellaneous) Q4
Quartile Score Medicine (miscellaneous) Q3
Scopus 438/167=2,6
Scite Score  
Scopus General Immunology and Microbiology 31/45 (Q3)
Scite Score Rank  
Scopus 0,760
SNIP
Days from  225
sumbission
to acceptance
Days from  118
acceptance
to publication
Acceptance 19%
Rate

2019  
Total Cites
WoS
485
Impact Factor 1,086
Impact Factor
without
Journal Self Cites
0,864
5 Year
Impact Factor
1,233
Immediacy
Index
0,286
Citable
Items
42
Total
Articles
40
Total
Reviews
2
Cited
Half-Life
5,8
Citing
Half-Life
7,7
Eigenfactor
Score
0,00059
Article Influence
Score
0,246
% Articles
in
Citable Items
95,24
Normalized
Eigenfactor
0,07317
Average
IF
Percentile
7,690
Scimago
H-index
27
Scimago
Journal Rank
0,352
Scopus
Scite Score
320/161=2
Scopus
Scite Score Rank
General Immunology and Microbiology 35/45 (Q4)
Scopus
SNIP
0,492
Acceptance
Rate
16%

 

Acta Microbiologica et Immunologica Hungarica
Publication Model Online only Hybrid
Submission Fee none
Article Processing Charge 1100 EUR/article
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Online subsscription: 652 EUR / 812 USD
Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content.
Purchase per Title Individual articles are sold on the displayed price.

Acta Microbiologica et Immunologica Hungarica
Language English
Size A4
Year of
Foundation
1954
Publication
Programme
2021 Volume 68
Volumes
per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 1217-8950 (Print)
ISSN 1588-2640 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Feb 2021 69 0 0
Mar 2021 50 0 0
Apr 2021 37 0 0
May 2021 47 0 0
Jun 2021 60 0 0
Jul 2021 77 0 0
Aug 2021 0 0 0