View More View Less
  • 1 The Republican Scientific and Practical Center for Epidemiology and Microbiology, Belarus
  • | 2 Medical Center “Nordin”, Belarus
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $784.00

The aim of this study was to evaluate the prevalence of Helicobacter pylori genotypes (vacA and cagPAI) directly in gastric biopsy specimens in patients with gastric diseases in Belarus. Gastric biopsies were collected from 461 patients with different gastrointestinal disorders: superficial gastritis (287 subjects), atrophy gastritis (59 subjects), erosive gastritis (47 subjects), duodenal ulcer disease (54 subjects), and stomach ulcer (14 subjects). PCR-based genotyping was used to detect s1a, s1b, s2, m1a, m1b, m2, cagM, cagA, and cagT genes. Overall prevalence of vacA s1a allele was 60.5% followed by m2 (47.1%) and m1a (37.5%). The analysis of data showed that genotype s1a/m1a was significantly more prevalent in patients with duodenal ulcer (21.4% vs. 45.1%, OR = 3.0, 95% CI = 1.5–6.1). The cagA gene was found with a high incidence in most patients with inflammatory diseases of stomach and duodenum. There was a significant increase in the frequency of cagT in patients with duodenal ulcer as compared to superficial gastritis. A high cagM prevalence was found in patients with atrophy gastritis and duodenal ulcer disease. All three island genes of pathogenicity of cagPAI are more often detected in patients with duodenal ulcer, which increases the risk of developing duodenal ulcer by 4.5 times.

  • 1.

    International Agency for Research on Cancers. Monographs on the Evaluation of Carcinogenic Risks to Humans. World Health Organization, Geneva, 1994, p. 61.

    • Search Google Scholar
    • Export Citation
  • 2.

    Atherton, J. C., Blaser, M. J.: Coadaptation of Helicobacter pylori and humans: Ancient history, modern implications. J Clin Invest 119, 24752487 (2009).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 3.

    Cao, D. M., Lu, Q. F., Li, S. B., Wang, J. P., Chen, Y. L., Huang, Y. Q., Bi, H. K.: Comparative genomics of H. pylori and non-pylori Helicobacter species to identify new regions associated with its pathogenicity and adaptability. Biomed Res Int 2016, 6106029 (2016).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 4.

    Thorell, K., Lehours, P., Vale, F. F.: Genomics of Helicobacter pylori. Helicobacter 22, e12409 (2017).

  • 5.

    Backert, S., Neddermann, M., Maubach, G., Naumann, M.: Pathogenesis of Helicobacter pylori infection. Helicobacter 21, 1925 (2016).

  • 6.

    Salama, N. R., Hartung, M. L., Müller, A.: Life in the human stomach: Persistence strategies of the bacterial pathogen Helicobacter pylori. Nat Rev Microbiol 11, 385399 (2013).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 7.

    Kao, C. Y., Sheu, B. S., Wu, J. J.: Helicobacter pylori infection: An overview of bacterial virulence factors and pathogenesis. Biomed J 39, 1423 (2016).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 8.

    Merino, E., Flores-Encarnación, M., Aguilar-Gutiérrez, G. R.: Functional interaction and structural characteristics of unique components of Helicobacter pylori T4SS. FEBS J 284, 35403549 (2017).

    • Search Google Scholar
    • Export Citation
  • 9.

    Oleastro, M., Menard, A., Santos, A., Lamouliatte, H., Monteiro, L., Barthelemy, Ph., Megraud, F.: Real-time PCR assay for rapid and accurate detection of point mutations conferring resistance to clarithromycin in Helicobacter pylori. J Clin Microbiol 41, 397402 (2003).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 10.

    Atherton, J. C., Cao, P., Peek, R. M. Jr., Tummuru, M. K., Blaser, M. J., Cover, T. L.: Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. Association of specific vacA types with cytotoxin production and peptic ulceration. J Biol Chem 270, 1777117777 (1995).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 11.

    Strobel, S., Bereswill, S., Balig, P., Allgaier, P., Sonntag, H. G., Kist, M.: Identification and analysis of a new vacA genotype variant of Helicobacter pylori in different patient groups in Germany. J Clin Microbiol 36, 12851289 (1998).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 12.

    White, J. R., Winter, J. A., Robinson, K.: Differential inflammatory response to Helicobacter pylori infection: Etiology and clinical outcomes. J Inflamm Res 8, 137147 (2015).

    • Search Google Scholar
    • Export Citation
  • 13.

    Alzahrani, Sh., Lina, T., Gonzalez, J., Pinchuk, I., Beswick, E., Reyes, V.: Effect of Helicobacter pylori on gastric epithelial cells. World J Gastroenterol 20, 1276712780 (2014).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 14.

    Oertli, M., Sundquist, M., Hitzler, I., Engler, D. B., Arnold, I. C., Reuter, S., Maxeiner, J., Hansson, M., Taube, Ch., Quiding-Järbrink, M., Müller, A.: DC-derived IL-18 drives Treg differentiation, murine Helicobacter pylori-specific immune tolerance, and asthma protection. J Clin Invest 122, 10821096 (2012).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 15.

    Isomoto, H., Moss, J., Hirayama, T.: Pleiotropic actions of Helicobacter pylori vacuolating cytotoxin, VacA. Tohoku J Exp Med 220, 314 (2010).

  • 16.

    Yamaoka, Y., Kodama, T., Gutierrez, O., Kim, J. G., Kashima, K., Graham, D. Y.: Relationship between Helicobacter pylori iceA, cagA, and vacA status and clinical outcome: Studies in four different countries. J Clin Microbiol 37, 22742279 (1999).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 17.

    Kolayli, F., Karadenizli, A., Bingöl, R., Schneider, T., Kist, M.: Differences of vacA alleles and cagA gene positivity of Helicobacter pylori strains isolated from two different countries: Turkey and Germany. Mikrobiyol Bul 46, 332334 (2012).

    • Search Google Scholar
    • Export Citation
  • 18.

    Boyanova, L., Markovska, R., Yordanov, D., Marina, M., Ivanova, K., Panayotov, S., Gergova, G., Mitov, I.: High prevalence of virulent Helicobacter pylori strains in symptomatic Bulgarian patients. Diagn Microbiol Infect Dis 64, 374380 (2009).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 19.

    Kauser, F., Hussain, M. A., Ahmed, I., Srinivas, S., Devi, S. M., Majeed, A. A., Rao, K. R., Khan, A. A., Sechi, L. A., Ahmed, N.: Comparative genomics of Helicobacter pylori isolates recovered from ulcer disease patients in England. BMC Microbiol 25, 32 (2005).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 20.

    Con, S. A., Takeuchi, H., Valerín, A. L., Con-Wong, R., Con-Chin, G. R., Con-Chin, V. G., Nishioka, M., Mena, F., Brenes, F., Yasuda, N., Araki, K., Sugiura, T.: Diversity of Helicobacter pylori cagA and vacA genes in Costa Rica: Its relationship with atrophic gastritis and gastric cancer. Helicobacter 12, 547552 (2007).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 21.

    Basso, D., Zambon, C. F., Letley, D. P., Stranges, A., Marchet, A., Rhead, J. L., Schiavon, S., Guariso, G., Ceroti, M., Nitti, D., Rugge, M., Plebani, M., Atherton, J. C.: Clinical relevance of Helicobacter pylori cagA and vacA gene polymorphisms. Gastroenterology 135, 9199 (2008).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 22.

    Almeida, N., Donato, M. M., Romãozinho, J. M., Luxo, C., Cardoso, O., Cipriano, M. A., Marinho, C., Fernandes, A., Sofia, C.: Correlation of Helicobacter pylori genotypes with gastric histopathology in the central region of a South-European country. Dig Dis Sci 60, 7485 (2015).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 23.

    Markovska, R., Boyanova, L., Yordanov, D., Stankova, P., Gergova, G., Mitov, I.: Status of Helicobacter pylori cag pathogenicity island (cagPAI) integrity and significance of its individual genes. Infect Genet Evol 59, 167171 (2018).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 24.

    Andreson, H., Loivukene, K., Sillakivi, T., Maaroos, H. I., Ustav, M., Peetsalu, A., Mikelsaar, M.: Association of cagA and vacA genotypes of Helicobacter pylori with gastric diseases in Estonia. J Clin Microbiol 40, 298300 (2002).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 25.

    Atherton, J. C., Peek, R. M., Tham, K. T., Cover, T. L., Blaser, M. J.: Clinical and pathological importance of heterogeneity in vacA, the vacuolating cytotoxin gene of Helicobacter pylori. Gastroenterology 112, 9299 (1997).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 26.

    Park, J. Y., Forman, D., Waskito, L. A., Yamaoka, Y., Crabtree, J. E.: Epidemiology of Helicobacter pylori and CagA-positive infections and global variations in gastric cancer. Toxins (Basel) 10, 163 (2018).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 27.

    Hatakeyama, M.: Oncogenic mechanisms of the Helicobacter pylori CagA protein. Nat Rev Cancer 4, 68869 (2004).

  • 28.

    Apostolopoulos, P., Vafiadis-Zouboulis, I., Tzivras, M., Kourtessas, D., Katsilambros, N., Archimandritis, A.: Helicobacter pylori (H. pylori) infection in Greece: The changing prevalence during a ten-year period and its antigenic profile. BMC Gastroenterol 16, 11 (2002).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 29.

    Caner, V., Yilmaz, M., Yonetci, N., Zencir, S., Karagenc, N., Kaleli, I., Bagci, H.: H. pylori iceA alleles are disease-specific virulence factors. World J Gastroenterol 13, 25812585 (2007).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 30.

    Pinto-Ribeiro, I., Ferreira, R. M., Batalha, S., Hlaing, T., Wong, S. I., Carneiro, F., Figueiredo, C.: Helicobacter pylori vacA genotypes in chronic gastritis and gastric carcinoma patients from Macau, China. Toxins (Basel) 8, E142 (2016).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 31.

    Aziz, F., Chen, X., Yang, X., Yan, Q.: Prevalence and correlation with clinical diseases of Helicobacter pylori cagA and vacA genotype among gastric patients from Northeast China. Biomed Res Int 2014, 142980 (2014).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 32.

    Johnson, E. M., Gaddy, J. A., Voss, B. J., Hennig, E. E., Cover, T. L.: Genes required for assembly of pili associated with the Helicobacter pylori cag type IV secretion system. Infect Immun 82, 34573470 (2014).

    • Crossref
    • Search Google Scholar
    • Export Citation
All Time Past Year Past 30 Days
Abstract Views 170 169 0
Full Text Views 29 5 0
PDF Downloads 13 3 0