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  • 1 Microbiology Department, Medical Research Institute, Alexandria University, Alexandria, Egypt
  • | 2 Department of Microbiology and Immunology, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria, Alexandria, Egypt
  • | 3 Alexandria University Hospital, Alexandria University, Alexandria, Egypt
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Acinetobacter baumannii has emerged as an important nosocomial pathogen due to its ability to survive in hospital settings and its antimicrobial resistance. It is one of the key pathogens in ventilator-associated pneumonia (VAP). The aim of this study was to characterize the mechanisms of quinolone resistance among A. baumannii isolates causing VAP and to investigate the presence of the novel abaQ gene among them. Quinolone-resistant A. baumannii isolates causing VAP were collected over a period of 4 months. Mutations within gyrA and parC were analyzed and the presence of qnrA, qnrB, qnrS, and abaQ was investigated genotypically. Twenty-one A. baumannii isolates were collected, most of them (76.2%) were extensively drug-resistant (XDR) and only one isolate (4.8%) was pandrug-resistant (PDR). All isolates showed high level of resistance to ciprofloxacin, while qnrA, qnrB and qnrS were absent among our isolates. This is the first report of A. baumannii isolates co-harboring Ser81Leu in gyrA and Ser84Leu in parC together with the novel abaQ gene. Interestingly, a new mutation in gyrA quinolone resistance-determining region Arg89Cys was detected among two of our isolates. The emergence of XDR and PDR isolates among A. baumannii causing VAP is an alarming threat.

  • 1.

    Deccache, Y., Irenge, L. M., Savov, E., Ariciuc, M., Macovei, A., Trifonova, A., Gergova, I., Ambroise, J., Vanhoof, R., Gala, J. L.: Development of a pyrosequencing assay for rapid assessment of quinolone resistance in Acinetobacter baumannii isolates. J Microbiol Methods 86, 115118 (2011).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 2.

    Erdem, H., Cag, Y., Gencer, S., Uysal, S., Karakurt, Z., Harman, R., Aslan, E., Mutlu-Yilmaz, E., Karabay, O., Uygun, Y., Ulug, M., Tosun, S., Dogru, A., Sener, A., Dogan, M., Hasbun, R., Durmus, G., Turan, H., Batirel, A., Duygu, F., Inan, A., Akkoyunlu, Y., Celebi, G., Ersoz, G., Guven, T., Dagli, O., Guler, S., Meric-Koc, M., Oncu, S., Rello, J.: Treatment of ventilator-associated pneumonia (VAP) caused by Acinetobacter: Results of prospective and multicenter ID-IRI study. Eur J Clin Microbiol Infect Dis (2019).

    • Search Google Scholar
    • Export Citation
  • 3.

    Cherkaoui, A., Emonet, S., Renzi, G., Schrenzel, J.: Characteristics of multidrug-resistant Acinetobacter baumannii strains isolated in Geneva during colonization or infection. Ann Clin Microbiol Antimicrob 14, 42 (2015).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 4.

    Kalil, A. C., Metersky, M. L., Klompas, M., Muscedere, J., Sweeney, D. A., Palmer, L. B., Napolitano, L. M., O’Grady, N. P., Bartlett, J. G., Carratala, J., El Solh, A. A., Ewig, S., Fey, P. D., File, T. M., Jr., Restrepo, M. I., Roberts, J. A., Waterer, G. W., Cruse, P., Knight, S. L., Brozek, J. L.: Management of adults with hospital-acquired and ventilator-associated pneumonia: 2016 clinical practice guidelines by the Infectious Diseases Society of America and the American Thoracic Society. Clin Infect Dis 63, e61e111 (2016).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 5.

    Nowak, J., Zander, E., Stefanik, D., Higgins, P. G., Roca, I., Vila, J., McConnell, M. J., Cisneros, J. M., Seifert, H., MagicBullet Working Group, W. P.: High incidence of pandrug-resistant Acinetobacter baumannii isolates collected from patients with ventilator-associated pneumonia in Greece, Italy and Spain as part of the MagicBullet clinical trial. J Antimicrob Chemother 72, 32773282 (2017).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 6.

    Huang, Y., Zhou, Q., Wang, W., Huang, Q., Liao, J., Li, J., Long, L., Ju, T., Zhang, Q., Wang, H., Xu, H., Tu, M.: Acinetobacter baumannii ventilator-associated pneumonia: Clinical efficacy of combined antimicrobial therapy and in vitro drug sensitivity test results. Front Pharmacol 10, 92 (2019).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 7.

    Hooper, D. C., Jacoby, G. A.: Mechanisms of drug resistance: Quinolone resistance. Ann N Y Acad Sci 1354, 1231 (2015).

  • 8.

    Asif, M., Alvi, I. A., Rehman, S. U.: Insight into Acinetobacter baumannii: Pathogenesis, global resistance, mechanisms of resistance, treatment options, and alternative modalities. Infect Drug Resist 11, 12491260 (2018).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 9.

    Perez-Varela, M., Corral, J., Aranda, J., Barbe, J.: Functional Characterization of AbaQ, a novel efflux pump mediating quinolone resistance in Acinetobacter baumannii. Antimicrob Agents Chemother 62, e00906e00918 (2018).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 10.

    Clinical and Laboratory Standards Institute: Performance Standards for Antimicrobial Susceptibility Testing, 28th ed. CLSI supplement M100. Clinical and Laboratory Standards Institute, Wayne, PA, 2018.

    • Search Google Scholar
    • Export Citation
  • 11.

    Turton, J. F., Woodford, N., Glover, J., Yarde, S., Kaufmann, M. E., Pitt, T. L.: Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species. J Clin Microbiol 44, 29742976 (2006).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 12.

    Srinivasan, V. B., Rajamohan, G., Pancholi, P., Stevenson, K., Tadesse, D., Patchanee, P., Marcon, M., Gebreyes, W. A.: Genetic relatedness and molecular characterization of multidrug resistant Acinetobacter baumannii isolated in central Ohio, USA. Ann Clin Microbiol Antimicrob 8, 21 (2009).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 13.

    Magiorakos, A. P., Srinivasan, A., Carey, R. B., Carmeli, Y., Falagas, M. E., Giske, C. G., Harbarth, S., Hindler, J. F., Kahlmeter, G., Olsson-Liljequist, B., Paterson, D. L., Rice, L. B., Stelling, J., Struelens, M. J., Vatopoulos, A., Weber, J. T., Monnet, D. L.: Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect 18, 268281 (2012).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 14.

    Ciginskiene, A., Dambrauskiene, A., Rello, J., Adukauskiene, D.: Ventilator-associated pneumonia due to drug-resistant Acinetobacter baumannii: Risk factors and mortality relation with resistance profiles, and independent predictors of in-hospital mortality. Medicina (Kaunas) 55, E49 (2019).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 15.

    Ostrer, L., Khodursky, R. F., Johnson, J. R., Hiasa, H., Khodursky, A.: Analysis of mutational patterns in quinolone resistance-determining regions of GyrA and ParC of clinical isolates. Int J Antimicrob Agents 53, 318324 (2019).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 16.

    Akter, F., Amin, M. R., Osman, K. T., Anwar, M. N., Karim, M. M., Hossain, M. A.: Ciprofloxacin-resistant Escherichia coli in hospital wastewater of Bangladesh and prediction of its mechanism of resistance. World J Microbiol Biotechnol 28, 827834 (2012).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 17.

    Conrad, S., Oethinger, M., Kaifel, K., Klotz, G., Marre, R., Kern, W. V.: gyrA Mutations in high-level fluoroquinolone-resistant clinical isolates of Escherichia coli. J Antimicrob Chemother 38, 443455 (1996).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 18.

    Lehn, N., Stower-Hoffmann, J., Kott, T., Strassner, C., Wagner, H., Kronke, M., Schneider-Brachert, W.: Characterization of clinical isolates of Escherichia coli showing high levels of fluoroquinolone resistance. J Clin Microbiol 34, 597602 (1996).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 19.

    Higuchi, S., Shikata, M., Chiba, M., Hoshino, K., Gotoh, N.: Characteristics of antibiotic resistance and sequence type of Acinetobacter baumannii clinical isolates in Japan and the antibacterial activity of DS-8587. J Infect Chemother 20, 256261 (2014).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 20.

    Bakour, S., Touati, A., Sahli, F., Ameur, A. A., Haouchine, D., Rolain, J. M.: Antibiotic resistance determinants of multidrug-resistant Acinetobacter baumannii clinical isolates in Algeria. Diagn Microbiol Infect Dis 76, 529531 (2013).

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 21.

    Hamed, S. M., Elkhatib, W. F., El-Mahallawy, H. A., Helmy, M. M., Ashour, M. S., Aboshanab, K. M. A.: Multiple mechanisms contributing to ciprofloxacin resistance among Gram negative bacteria causing infections to cancer patients. Sci Rep 8, 12268 (2018).

    • Crossref
    • Search Google Scholar
    • Export Citation

 

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Senior editors

Editor-in-Chief: Prof. Dóra Szabó (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Managing Editor: Dr. Béla Kocsis (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Co-editor: Dr. Andrea Horváth (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Editorial Board

  • Prof. Éva ÁDÁM (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Sebastian AMYES (Department of Medical Microbiology, University of Edinburgh, Edinburgh, UK.)
  • Dr. Katalin BURIÁN (Institute of Clinical Microbiology University of Szeged, Szeged, Hungary; Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.)
  • Dr. Orsolya DOBAY (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Ildikó Rita DUNAY (Institute of Inflammation and Neurodegeneration, Medical Faculty, Otto-von-Guericke University, Magdeburg, Germany; Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany)
  • Prof. Levente EMŐDY(Department of Medical Microbiology and Immunology, University of Pécs, Pécs, Hungary.)
  • Prof. Anna ERDEI (Department of Immunology, Eötvös Loránd University, Budapest, Hungary, MTA-ELTE Immunology Research Group, Eötvös Loránd University, Budapest, Hungary.)
  • Prof. Éva Mária FENYŐ (Division of Medical Microbiology, University of Lund, Lund, Sweden)
  • Prof. László FODOR (Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary)
  • Prof. József KÓNYA (Department of Medical Microbiology, University of Debrecen, Debrecen, Hungary)
  • Prof. Yvette MÁNDI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Károly MÁRIALIGETI (Department of Microbiology, Eötvös Loránd University, Budapest, Hungary)
  • Prof. János MINÁROVITS (Department of Oral Biology and Experimental Dental Research, University of Szeged, Szeged, Hungary)
  • Prof. Béla NAGY (Centre for Agricultural Research, Institute for Veterinary Medical Research, Budapest, Hungary.)
  • Prof. István NÁSZ (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Kristóf NÉKÁM (Hospital of the Hospitaller Brothers in Buda, Budapest, Hungary.)
  • Dr. Eszter OSTORHÁZI (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Rozália PUSZTAI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Peter L. RÁDY (Department of Dermatology, University of Texas, Houston, Texas, USA)
  • Prof. Éva RAJNAVÖLGYI (Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary)
  • Prof. Ferenc ROZGONYI (Institute of Laboratory Medicine, Semmelweis University, Budapest, Hungary)
  • Prof. Zsuzsanna SCHAFF (2nd Department of Pathology, Semmelweis University, Budapest, Hungary)
  • Prof. Joseph G. SINKOVICS (The Cancer Institute, St. Joseph’s Hospital, Tampa, Florida, USA)
  • Prof. Júlia SZEKERES (Department of Medical Biology, University of Pécs, Pécs, Hungary.)
  • Prof. Mária TAKÁCS (National Reference Laboratory for Viral Zoonoses, National Public Health Center, Budapest, Hungary.)
  • Prof. Edit URBÁN (Department of Medical Microbiology and Immunology University of Pécs, Pécs, Hungary; Institute of Translational Medicine, University of Pécs, Pécs, Hungary.)

 

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2020  
Total Cites 662
WoS
Journal
Impact Factor
2,048
Rank by Immunology 145/162(Q4)
Impact Factor Microbiology 118/137 (Q4)
Impact Factor 1,904
without
Journal Self Cites
5 Year 0,671
Impact Factor
Journal  0,38
Citation Indicator  
Rank by Journal  Immunology 146/174 (Q4)
Citation Indicator  Microbiology 120/142 (Q4)
Citable 42
Items
Total 40
Articles
Total 2
Reviews
Scimago 28
H-index
Scimago 0,439
Journal Rank
Scimago Immunology and Microbiology (miscellaneous) Q4
Quartile Score Medicine (miscellaneous) Q3
Scopus 438/167=2,6
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Scopus General Immunology and Microbiology 31/45 (Q3)
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Scopus 0,760
SNIP
Days from  225
sumbission
to acceptance
Days from  118
acceptance
to publication
Acceptance 19%
Rate

2019  
Total Cites
WoS
485
Impact Factor 1,086
Impact Factor
without
Journal Self Cites
0,864
5 Year
Impact Factor
1,233
Immediacy
Index
0,286
Citable
Items
42
Total
Articles
40
Total
Reviews
2
Cited
Half-Life
5,8
Citing
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7,7
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0,00059
Article Influence
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0,246
% Articles
in
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95,24
Normalized
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0,07317
Average
IF
Percentile
7,690
Scimago
H-index
27
Scimago
Journal Rank
0,352
Scopus
Scite Score
320/161=2
Scopus
Scite Score Rank
General Immunology and Microbiology 35/45 (Q4)
Scopus
SNIP
0,492
Acceptance
Rate
16%

 

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Acta Microbiologica et Immunologica Hungarica
Language English
Size A4
Year of
Foundation
1954
Publication
Programme
2021 Volume 68
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per Year
1
Issues
per Year
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Founder Magyar Tudományos Akadémia
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ISSN 1217-8950 (Print)
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