Search for other papers by Aliakbar Rezaei in
Current site
Google Scholar
PubMed
Search for other papers by Hossein Fazeli in
Current site
Google Scholar
PubMed
Search for other papers by Jamshid Faghri in
Current site
Google Scholar
PubMed
Abstract
This study investigated carbapenem resistance among Acinetobacter baumannii isolated from respiratory specimens. Epidemiological relationship of the isolates was also evaluated. In this study, 81 respiratory specimens of A. baumannii from AL Zahra Hospital were confirmed by phenotypic and genotypic methods. Antimicrobial susceptibility was performed by disc diffusion method. Carbapenem resistance genes were identified by PCR. The isolates were typed by RAPD-PCR and multilocus sequence typing (MLST) methods. All isolates were resistant to imipenem and 80 isolates to meropenem. Frequency of oxacillinase genes was as follows: blaOXA-23 gene was positive in 74 (91.3%), blaOXA-24 gene in 50 (61.7%) and blaOXA-58 was not found in any isolates. On the other hand 22 (27.2%) isolates contained blaIMP-1, 3 (3.7%) isolates contained blaIMP-2 gene, 5 (6.2%) isolates contained blaVIM-1, 4 (5%) isolates had blaVIM-2 and none of the isolates had blaSIM-1 gene. RAPD-PCR typing identified 16 different patterns, with one pattern being the most frequent one in 26 isolates. In MLST 6 different sequence types were identified, the most predominant being ST2 belonging to clonal complex 2. The results of this study showed high resistance to carbapenems as well as high abundance of oxacillinase genes.
-
[1]
Gusatti CDS, Bertholdo LM, Otton LM, Marchetti DP, Ferreira AE, Corção G. First occurrence of blaOXA-58 in Acinetobacter baumannii isolated from a clinical sample in Southern Brazil. Braz J Microbiol 2012; 43(1): 243–6.
-
[2]
Azizi O, Shahcheraghi F, Salimizand H, Modarresi F, Shakibaie MR, Mansouri S, et al.. Molecular analysis and expression of bap gene in biofilm-forming multi-drug-resistant Acinetobacter baumannii. Rep. Biochem. Mol. Biol. 2016; 5(1): 62.
-
[3]
Diancourt L, Passet V, Nemec A, Dijkshoorn L, Brisse S. The population structure of Acinetobacter baumannii: expanding multiresistant clones from an ancestral susceptible genetic pool. PloS One 2010; 5: 4.
-
[4]
Falagas ME, Bliziotis IA, Siempos II. Attributable mortality of Acinetobacter baumannii infections in critically ill patients: a systematic review of matched cohort and case-control studies. Critical Care 2006; 10(2): R48.
-
[5]
Farshadzadeh Z, Hashemi FB, Rahimi S, Pourakbari B, Esmaeili D, Haghighi MA, et al.. Wide distribution of carbapenem resistant Acinetobacter baumannii in burns patients in Iran. Front Microbiol 2015; 6: 1146.
-
[6]
Hagihara M, Housman ST, Nicolau DP, Kuti JL. In vitro pharmacodynamics of polymyxin B and tigecycline alone and in combination against carbapenem-resistant Acinetobacter baumannii. Antimicrob Agents Chemother 2014; 58(2): 874–9.
-
[7]
Halaji M, Rezaei A, Zalipoor M, Faghri J. Investigation of class I, II, and III integrons among Acinetobacter Baumannii isolates from hospitalized patients in Isfahan, Iran. Oman Med J 2018; 33(1): 37.
-
[8]
Hall GS. Bailey & Scott's diagnostic microbiology. 13th ed. American Society for Clinical Pathology; 2013.
-
[9]
Hammerum AM, Hansen F, Skov MN, Stegger M, Andersen PS, Holm A, et al.. Investigation of a possible outbreak of carbapenem-resistant Acinetobacter baumannii in Odense, Denmark using PFGE, MLST and whole-genome-based SNPs. J Antimicrobial Chemother 2015; 70(7): 1965–8.
-
[10]
Higgins PG, Pérez-Llarena FJ, Zander E, Fernández A, Bou G, Seifert H. OXA-235, a novel class D β-lactamase involved in resistance to carbapenems in Acinetobacter baumannii. Antimicrob Agents Chemother 2013; 57(5): 2121–6.
-
[11]
Hojabri Z, Pajand O, Bonura C, Aleo A, Giammanco A, Mammina C. Molecular epidemiology of Acinetobacter baumannii in Iran: endemic and epidemic spread of multiresistant isolates. J Antimicrob Chemother 2014; 69(9): 2383–7.
-
[12]
Huang G, Yin S, Gong Y, Zhao X, Zou L, Jiang B, et al.. Multilocus sequence typing analysis of carba.penem-resistant Acinetobacter baumannii in a Chinese burns institute. Front Microbiol 2016; 7: 1717.
-
[13]
Karah N, Sundsfjord A, Towner K, Samuelsen Ø. Insights into the global molecular epidemiology of carbapenem non-susceptible clones of Acinetobacter baumannii. Drug Resistance Updates 2012; 15(4): 237–47.
-
[14]
Karmostaji A, Javadpour S, Davoodian P, Moradi N. In vitro activity of tigecycline and colistin against clinical isolates of Acinetobacter baumannii in hospitals in Tehran and Bandar-Abbas, Iran. Electronic physician 2014; 6(3): 919.
-
[15]
Lee K, Chong Y, Shin H, Kim Y, Yong D, Yum J. Modified Hodge and EDTA-disk synergy tests to screen metallo-β-lactamase-producing strains of Pseudomonas and Acinetobactet species. Clin Microbiol Infect 2001; 7(2): 88–91.
-
[16]
Bartua SG l, Seifert H, Hippler C. Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii. J Clin Microbiol 2005; 43: 4382–4390.
-
[17]
Mirnejad R, Mostofi S, Masjedian F. Antibiotic resistance and carriage class 1 and 2 integrons in clinical isolates of Acinetobacter baumannii from Tehran, Iran. Asian Pac J Trop Biomed 2013; 3(2): 140–5.
-
[18]
Peleg AY, Seifert H, Paterson DL. Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev 2008; 21(3): 538–82.
-
[19]
Rezaei A, Fazeli H, Halaji M, Moghadampour M, Faghri J. Prevalence of metallo-beta-lactamase producing Acinetobacter baumannii isolated from intensive care unit in tertiary care hospitals. Ann Ig 2018; 30(4): 330–6.
-
[20]
Royer S, Faria ALS, Seki LM, Chagas TPG, Campos PAD, Batistão DWDF, et al.. Spread of multidrug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa clones in patients with ventilator-associated pneumonia in an adult intensive care unit at a university hospital. Braz J Inf Dis 2015; 19(4): 350–7.
-
[21]
Saffari F, Monsen T, Karmostaji A, Azimabad FB, Widerström M. Significant spread of extensively drug-resistant Acinetobacter baumannii genotypes of clonal complex 92 among intensive care unit patients in a university hospital in southern Iran. J Med Microbiol 2017; 66(11): 1656–62.
-
[22]
Sevillano E, Gallego L. Molecular techniques for detection and control of nosocomial infections caused by Acinetobacter baumannii. Science against microbial pathogens: communicating current research and technological advances. Spain: Formatex Research Center Badajoz; 2011. pp. 495–503.
-
[23]
Tarashi S, Goudarzi H, Erfanimanesh S, Pormohammad A, Hashemi A. Phenotypic and molecular detection of metallo-beta-lactamase genes among imipenem resistant Pseudomonas aeruginosa and Acinetobacter baumannii strains isolated from patients with burn injuries. Arch Clin Inf Dis 2016; 11: 4.
-
[24]
Turton JF, Woodford N, Glover J, Yarde S, Kaufmann ME, Pitt TL. Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species. J Clin Microbiol 2006; 44(8): 2974–6.
-
[25]
Vincent J-L. Nosocomial infections in adult intensive-care units. The Lancet 2003; 361(9374): 2068–77.
-
[26]
Yelken B, Erkasap N, Bayram B, Us T, Ceylan I, Özkurt M, et al.. Epidemiology of Acinetobacter baumannii isolates from patients with severe sepsis in anesthesia intensive care unit/Anestezi Yogun Bakim Ünitesinde Agir Sepsisli Hastalardan Izole Edilen Acinetobacter baumannii Izolatlarinin Epidemiyolojisi. FABAD J Pharm Sci 2011; 36(2): 63.
-
[27]
Zangeneh Z, Eftekhar F. Study of class-D oxacillinase types in imipenem-resistant Acinetobacter baumannii clinical isolates by Rapd-Pcr. Iran J Public Health 2014; 43(2): 37.
-
[28]
Zowawi HM, Sartor AL, Sidjabat HE, Balkhy HH, Walsh TR, Al Johani SM, et al.. Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii isolates in the Gulf Cooperation Council States: dominance of OXA-23-type producers. J Clin Microbiol 2015; 53(3): 896–903.
Please, download the file from HERE
Editorial Office:
Akadémiai Kiadó Zrt.
Budafoki út 187-187, A/3, H-1117 Budapest, Hungary
Editorial Correspondence:
Acta Microbiologica et Immunologica Hungarica
Institute of Medical Microbiology
Semmelweis University
P.O. Box 370
H-1445 Budapest, Hungary
Phone: + 36 1 459 1500 ext. 56101
Fax: (36 1) 210 2959
E-mail: amih@med.semmelweis-univ.hu
Indexing and Abstracting Services:
- Biological Abstracts
- BIOSIS Previews
- CAB Abstracts
- CABELLS Journalytics
- Chemical Abstracts
- Global Health
- Index Medicus
- Index Veterinarius
- Medline
- Referativnyi Zhurnal
- SCOPUS
- Science Citation Index Expanded
| 2023 | |
|---|---|
| Web of Science | |
| Journal Impact Factor | 1.3 |
| Rank by Impact Factor | Q4 (Immunology) |
| Journal Citation Indicator | 0.31 |
| Scopus | |
| CiteScore | 2.3 |
| CiteScore rank | Q3 (Infectious Diseases) |
| SNIP | 0.389 |
| Scimago | |
| SJR index | 0.308 |
| SJR Q rank | Q3 |
| Acta Microbiologica et Immunologica Hungarica | |
|---|---|
| Publication Model | Hybrid |
| Submission Fee | none |
| Article Processing Charge | 1100 EUR/article (only for OA publications) |
| Regional discounts on country of the funding agency | World Bank Lower-middle-income economies: 50% World Bank Low-income economies: 100% |
| Further Discounts | Editorial Board / Advisory Board members: 50% Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100% |
| Subscription fee 2025 | Online subsscription: 772 EUR / 848 USD Print + online subscription: 860 EUR / 944 USD |
| Subscription Information | Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content. |
| Purchase per Title | Individual articles are sold on the displayed price. |
| Acta Microbiologica et Immunologica Hungarica | |
|---|---|
| Language | English |
| Size | A4 |
| Year of Foundation |
1954 |
| Volumes per Year |
1 |
| Issues per Year |
4 |
| Founder | Magyar Tudományos Akadémia |
| Founder's Address |
H-1051 Budapest, Hungary, Széchenyi István tér 9. |
| Publisher | Akadémiai Kiadó |
| Publisher's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. |
| Responsible Publisher |
Chief Executive Officer, Akadémiai Kiadó |
| ISSN | 1217-8950 (Print) |
| ISSN | 1588-2640 (Online) |
Monthly Content Usage
| Abstract Views | Full Text Views | PDF Downloads | |
|---|---|---|---|
| Apr 2024 | 39 | 0 | 0 |
| May 2024 | 22 | 0 | 0 |
| Jun 2024 | 30 | 0 | 1 |
| Jul 2024 | 41 | 0 | 0 |
| Aug 2024 | 21 | 0 | 0 |
| Sep 2024 | 40 | 0 | 0 |
| Oct 2024 | 0 | 0 | 0 |
Copyright Akadémiai Kiadó AKJournals is the trademark of Akadémiai Kiadó's journal publishing business branch.