Search for other papers by Elvan Hortaç İştar in
Current site
Google Scholar
PubMed
Search for other papers by Hikmet Eda Alışkan in
Current site
Google Scholar
PubMed
Search for other papers by Jülide Sedef Göçmen in
Current site
Google Scholar
PubMed
Abstract
After the first description of OXA-48 type carbapenemase, it has become endemic in Europe, Mediterranean and North African countries in a short time. OXA-48 carbapenemase is the most difficult type to determine and accurate diagnosis is crucial especially in endemic areas.
The CarbaNP test was described as a rapid phenotypic evaluation method of carbapenemases activity. Sensitivity and specifity of this test were high within all carbapenemases genes. In our study, we evaluated the efficacy of CarbaNP test in routine laboratories located in an endemic area of OXA-48 producing Enterobacterales.
A total of 53 Enterobacterales isolates were included in this study. Antimicrobial susceptibility of the isolates to imipenem, meropenem and ertapenem was determined. Polymerase Chain Reaction (PCR) was carried out for the detection of carbapenemases genes (blaKPC, blaNDM, blaBIC, blaIMP, blaVIM, blaSPM, blaAIM, blaDIM, blaGIM, blaSIM, and blaOXA-48). The Carba NP test was performed as in the protocol described previously.
Altogether 31 isolates (58.4%) were blaOXA-48 positive (18 Klebsiella pneumoniae, 8 Escherichia coli, 2 Serratia marcescens, 1 Enterobacter aerogenes, 1 Pantoea agglomerans and 1 Morganella morganii). Among these isolates 3 (5.6%) and 2 (3.7%) isolates were also positive for blaVIM and blaSPM, respectively.
The sensitivity and specifity of CarbaNP test were found 64.5, and 68.2% respectively. It was observed that determination of positive isolates is hard to distinguish and subjective.
The CarbaNP test has suboptimal results and low of sensitivity and specifity for detection of OXA-48 producing Enterobacterales, and not suitable for detection of blaOXA-48 positive isolates in routine laboratories in endemic areas.
-
1.
Naas T, Nordmann P. Analysis of a carbapenem-hydrolyzing class A beta-lactamase from Enterobacter cloacae and of its LysR-type regulatory protein. Proc Natl Acad Sci USA 1994; 91: 7693–7.
-
2.
Giske CG, Sundsfjord AS, Kahlmeter G, Woodford N, Nordmann P, Paterson DL, et al.. Redefining extended-spectrum β-lactamases: balancing science and clinical need. J Antimicrob Chemother 2009; 63: 1–4.
-
3.
Poirel L, Heritier C, Tolun V, Nordmann P. Emergence of oxacillinase-mediated resistance to imipenem in Klebsiella pneumoniae. Antimicrob Agents Chemother 2004; 48: 15–22.
-
4.
Carrer A, Poirel L, Yılmaz M, Akan OA, Feriha C, Cuzon G, et al.. Spread of OXA-48-encoding plasmid in Turkey and beyond. Antimicrob Agents Chemother 2010; 54: 1369–73.
-
5.
Cuzon G, Ouanich J, Gondret R, Naas T, Nordmann P. Outbreak of OXA-48-positive carbapenem-resistant Klebsiella pneumoniae isolates in France. Antimicrob Agents Chemother 2011; 55: 2420–3.
-
6.
Moquet O, Bouchiat C, Kinana A, Seck A, Arouna O, Bercion R, et al.. Class D OXA-48 carbapenemase in multidrug-resistant Enterobacteriaceae, Senegal. Emerg Infect Dis 2011; 17: 143–4.
-
7.
Dimou V, Dhanji H, Pike R, Livermore DM, Woodford N. Characterization of Enterobacteriaceae producing OXA-48-like carbapenemases in the UK. J Antimicrob Chemother 2012; 67: 1660–5.
-
8.
Rodríguez-Lucas C, Rodicio MR, Rosete Y, Fernández J. Prospective evaluation of an easy and reliable work-flow for the screening of OXA-48- producing Klebsiella pneumoniae in endemic settings. J Hosp Infect 2020; 105: 659–62.
-
9.
Djahmi N, Dunyach-Remy C, Pantel A, Dekhil M, Sotto A, Lavigne JP. Epidemiology of carbapenemase-producing Enterobacteriaceae and Acinetobacter baumannii in mediterranean countries. Biomed Res Int 2014; 2014: 305784.
-
10.
Albiger B, Glasner C, Struelens MJ, Grundmann H, Monnet DL, Eckmanns T. Carbapenemase-producing Enterobacteriaceae in Europe: assessment by national experts from 38 countries, May 2015. Euro Surveill 2015; 20.
-
11.
Poirel L, Naas T, Nordmann P. Diversity, epidemiology, and genetics of class D beta-lactamases. Antimicrob Agents Chemother 2010; 54: 24–38.
-
12.
The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 10.0; 2020. https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_10.0_Breakpoint_Tables.pdf.
-
13.
Nordmann P, Poirel L, Dortet L. Rapid detection of carbapenemase-producing Enterobacteriaceae. Emerg Infect Dis 2012; 18: 1503–7.
-
14.
Nordmann P, Sadek M, Demord A, Poirel L. NitroSpeed-carba NP test for rapid detection and differentiation between different classes of carbapenemases in Enterobacterales. J Clin Microbiol 2020; 58: e00932–20.
-
15.
Poirel L, Walsh TR, Cuvillier V, Nordmann P. Multiplex PCR for detection of acquired carbapenemase genes. Diagn Microbiol Infect Dis 2011; 70: 119–23.
-
16.
Düzgün AÖ, Saral A. Next-generation sequencing of plasmid carrying blaOXA-48 in Klebsiella pneumoniae from Turkey. Acta Microbiol Immunol Hung 2019; 66: 261–72.
-
17.
Lascols C, Hackel M, Marshall SH, Hujer AM, Bouchillon S, Badal R, et al.. Increasing prevalence and dissemination of NDM-1 metallo-β-lactamase in India: data from the SMART study (2009). J Antimicrob Chemother 2011; 66: 1992–7.
-
18.
Poirel L, Ros A, Carrër A, Fortineau N, Carricajo A, Berthelot P, et al.. Cross-border transmission of OXA-48-producing Enterobacter cloacae from Morocco to France. J Antimicrob Chemother 2011; 66: 1181–2.
-
19.
Cantón R, Akóva M, Carmeli Y, Giske CG, Glupczynski Y, Gniadkowski M, et al.. Rapid evolution and spread of carbapenemases among Enterobacteriaceae in Europe. Clin Microbiol Infect 2012; 18: 413–31.
-
20.
Balkan II, Aygun G, Aydın S, Mutcalı SI, Kara Z, Kuskucu M, et al.. Blood stream infections due to OXA-48-like carbapenemase-producing Enterobacteriaceae: treatment and survival. Int J Infect Dis 2014; 26: 51–6.
-
21.
Räisänen K, Lyytikäinen O, Kauranen J, Tarkka E, Forsblom-Helander B, Grönroos JO, et al.. Molecular epidemiology of carbapenemase-producing Enterobacterales in Finland, 2012-2018. Eur J Clin Microbiol Infect Dis 2020; 39: 1651–6.
-
22.
Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. 30th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute; 2020.
-
23.
Hrabák J, Chudáčková E, Papagiannitsis CC. Detection of carbapenemases in Enterobacteriaceae: a challenge for diagnostic microbiological laboratories. Clin Microbiol Infect 2014; 20: 839–53.
-
24.
Dortet L, Poirel L, Nordmann P. Rapid identification of carbapenemase types in Enterobacteriaceae and Pseudomonas spp. by using a biochemical test. Antimicrob Agents Chemother 2012; 56: 6437–40.
-
25.
Österblad M, Hakanen AJ, Jalava J. Evaluation of the Carba NP test for carbapenemase detection. Antimicrob Agents Chemother 2014; 58: 7553–6.
-
26.
Dortet L, Bréchard L, Cuzon G, Poirel L, Nordmann P. Strategy for rapid detection of carbapenemase-producing Enterobacteriaceae. Antimicrob Agents Chemother 2014; 58: 2441–5.
-
27.
Yusuf E, Van Der Meeren S, Schallier A, Piérard D. Comparison of the Carba NP test with the rapid CARB screen kit for the detection of carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa. Eur J Clin Microbiol Infect Dis 2014; 33: 2237–40.
-
28.
Tijet N, Boyd D, Patel SN, Mulvey MR, Melano RG. Evaluation of the Carba NP test for rapid detection of carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa. Antimicrob Agents Chemother 2013; 57: 4578–80.
-
29.
Vasoo S, Cunningham SA, Kohner PC, Simner PJ, Mandrekar JN, Lolans K, et al.. Comparison of a novel, rapid chromogenic biochemical assay, the Carba NP test, with the modified Hodge test for detection of carbapenemase-producing Gram-negative bacilli. J Clin Microbiol 2013; 51: 3097–101.
-
30.
Tijet N, Boyd D, Patel SN, Mulvey MR, Melano RG. Reply to “Further proofs of concept for the Carba NP test”. Antimicrob Agents Chemother 2014; 58: 1270.
-
31.
Poirel L, Potron A, Nordmann P. OXA-48-like carbapenemases: the phantom menace. J Antimicrob Chemother 2012; 67: 1597–606.
-
32.
Chan WW, Campbell L, Doyle D, Pitout JD. Rapid detection of Enterobacterales that produce carbapenemases. Diagn Microbiol Infect Dis 2020; 98: 115120.
-
33.
Dortet L, Poirel L, Errera C, Nordmann P. CarbAcinetoNP test for rapid detection of carbapenemase-producing Acinetobacter spp. J Clin Microbiol 2014; 52: 2359–64.
Please, download the file from HERE
Editorial Office:
Akadémiai Kiadó Zrt.
Budafoki út 187-187, A/3, H-1117 Budapest, Hungary
Editorial Correspondence:
Acta Microbiologica et Immunologica Hungarica
Institute of Medical Microbiology
Semmelweis University
P.O. Box 370
H-1445 Budapest, Hungary
Phone: + 36 1 459 1500 ext. 56101
Fax: (36 1) 210 2959
E-mail: amih@med.semmelweis-univ.hu
Indexing and Abstracting Services:
- Biological Abstracts
- BIOSIS Previews
- CAB Abstracts
- CABELLS Journalytics
- Chemical Abstracts
- Global Health
- Index Medicus
- Index Veterinarius
- Medline
- Referativnyi Zhurnal
- SCOPUS
- Science Citation Index Expanded
| 2023 | |
|---|---|
| Web of Science | |
| Journal Impact Factor | 1.3 |
| Rank by Impact Factor | Q4 (Immunology) |
| Journal Citation Indicator | 0.31 |
| Scopus | |
| CiteScore | 2.3 |
| CiteScore rank | Q3 (Infectious Diseases) |
| SNIP | 0.389 |
| Scimago | |
| SJR index | 0.308 |
| SJR Q rank | Q3 |
| Acta Microbiologica et Immunologica Hungarica | |
|---|---|
| Publication Model | Hybrid |
| Submission Fee | none |
| Article Processing Charge | 1100 EUR/article (only for OA publications) |
| Regional discounts on country of the funding agency | World Bank Lower-middle-income economies: 50% World Bank Low-income economies: 100% |
| Further Discounts | Editorial Board / Advisory Board members: 50% Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100% |
| Subscription fee 2025 | Online subsscription: 772 EUR / 848 USD Print + online subscription: 860 EUR / 944 USD |
| Subscription Information | Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content. |
| Purchase per Title | Individual articles are sold on the displayed price. |
| Acta Microbiologica et Immunologica Hungarica | |
|---|---|
| Language | English |
| Size | A4 |
| Year of Foundation |
1954 |
| Volumes per Year |
1 |
| Issues per Year |
4 |
| Founder | Magyar Tudományos Akadémia |
| Founder's Address |
H-1051 Budapest, Hungary, Széchenyi István tér 9. |
| Publisher | Akadémiai Kiadó |
| Publisher's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. |
| Responsible Publisher |
Chief Executive Officer, Akadémiai Kiadó |
| ISSN | 1217-8950 (Print) |
| ISSN | 1588-2640 (Online) |
Monthly Content Usage
| Abstract Views | Full Text Views | PDF Downloads | |
|---|---|---|---|
| Apr 2024 | 31 | 1 | 1 |
| May 2024 | 62 | 0 | 0 |
| Jun 2024 | 49 | 0 | 1 |
| Jul 2024 | 59 | 0 | 0 |
| Aug 2024 | 50 | 0 | 0 |
| Sep 2024 | 36 | 0 | 0 |
| Oct 2024 | 0 | 0 | 0 |
Copyright Akadémiai Kiadó AKJournals is the trademark of Akadémiai Kiadó's journal publishing business branch.