Authors:
Qingyu Wang Department of Clinical Laboratory, Weifang People's Hospital, Weifang, Shandong, China

Search for other papers by Qingyu Wang in
Current site
Google Scholar
PubMed
Close
,
Xinyi Wang Clinical Medicine, Shandong First Medical University, Taian, Shandong, China

Search for other papers by Xinyi Wang in
Current site
Google Scholar
PubMed
Close
,
Chunling Chen Department of Clinical Laboratory, Weifang Desheng Internal Medicine Hospital, Shandong, China

Search for other papers by Chunling Chen in
Current site
Google Scholar
PubMed
Close
,
Li Zhao Department of Clinical Laboratory, Zhucheng Longdu Hospital, Shandong, China

Search for other papers by Li Zhao in
Current site
Google Scholar
PubMed
Close
,
Jie Ma Department of Clinical Laboratory, Weifang People's Hospital, Weifang, Shandong, China

Search for other papers by Jie Ma in
Current site
Google Scholar
PubMed
Close
, and
Kai Dong Emergency Department, Weifang People's Hospital, Weifang, Shandong, China

Search for other papers by Kai Dong in
Current site
Google Scholar
PubMed
Close
https://orcid.org/0009-0000-8945-4161
Restricted access

Abstract

Object of our study was to analyze the carriage of resistance genes in carbapenem-resistant Raoultella planticola (CRRP) by whole genome sequencing (WGS). Three strains of CRRP (named WF0027, WF3597 and WF3648) were collected for clinical analysis and susceptibility of antimicrobial agents was determined. The WGS of three strains was done by Illumina platform and strain identification was performed by average nucleotide identity, and the antibiotic resistance genes carried by the three strains were detected by ABRicate software. Whole genome data of 46 CRRP strains were downloaded from the National Center for Biotechnology Information (NCBI) database, and the evolutionary tree was constructed by genomic single nucleotide polymorphism together with this study strains. Antimicrobial susceptibility testing revealed that WF3597 and WF3648 were susceptible to tigecycline and colistin, while exhibited resistance to 24 antimicrobial agents. WF0027 was resistant to 18 antimicrobial agents. A total of 25 resistance genes were identified using ABRicate software. WF0027 carried blaIMP-8, whereas WF3597 and WF3648 carried blaNDM-1 carbapenem resistance gene. As predicted by the PlasmidFinder, WF3597 and WF3648 carried one plasmid IncFII(p14)_1_p14, whereas WF0027 carried five plasmids. Evolutionary tree results show all strains are clustered into six groups, the strains WF3597 and WF3648 belonged to the same evolutionary group (E clade) and WF0027 belonged to the F clade. Three CRRP strains in our study carried carbapenem resistance genes (blaNDM-1 or blaIMP-8) and were resistant to multiple antimicrobial agents, posing a significant challenge for clinical treatment.

  • 1.

    Ershadi A, Weiss E, Verduzco E, Chia D, Sadigh M. Emerging pathogen: a case and review of Raoultella planticola. Infection 2014; 42(6): 10431046.

    • Search Google Scholar
    • Export Citation
  • 2.

    Ferragut C, Izard D, Gavini F, Kersters K, Deley J, Leclerc H. Klebsiella trevisanii: a new species from water and soil. Int J Syst Bacteriol 1983; 33: 133142.

    • Search Google Scholar
    • Export Citation
  • 3.

    Freney J, Fleurette J, Gruer LD, Desmonceaux M, Gavini F, Leclerc H. Klebsiella trevisanii colonisation and septicaemia. Lancet 1984; 1(8382): 909.

    • Search Google Scholar
    • Export Citation
  • 4.

    Olson DS Jr, Asare K, Lyons M, Hofinger DM. A novel case of Raoultella planticola urinary tract infection. Infection 2013 Feb; 41(1): 259261.

    • Search Google Scholar
    • Export Citation
  • 5.

    Cho YJ, Jung EJ, Seong JS, Woo YM, Jeong BJ, Kang YM, et al. A case of pneumonia caused by Raoultella planticola. Tuberc Respir Dis (Seoul) 2016 Jan; 79(1): 4245.

    • Search Google Scholar
    • Export Citation
  • 6.

    Lam PW, Salit IE. Raoultella planticola bacteremia following consumption of seafood. Can J Infect Dis Med Microbiol 2014 Jul; 25(4): e83e84.

    • Search Google Scholar
    • Export Citation
  • 7.

    Zeng M, Xia J, Zong Z, Shi Y, Ni Y, Hu F, et al. Guidelines for the diagnosis, treatment, prevention and control of infections caused by carbapenem-resistant gram-negative bacilli. J Microbiol Immunol Infect 2023; S1684-1182(23): 00036-1.

    • Search Google Scholar
    • Export Citation
  • 8.

    Armin S, Fallah F, Karimi A, Karbasiyan F, Alebouyeh M, Rafiei Tabatabaei S, et al. Antibiotic susceptibility patterns for carbapenem-resistant Enterobacteriaceae. Int J Microbiol 2023; 2023: 8920977.

    • Search Google Scholar
    • Export Citation
  • 9.

    Castanheira M, Deshpande LM, DiPersio JR, Kang J, Weinstein MP, Jones RN. First descriptions of blaKPC in Raoultella spp. (R. planticola and R. ornithinolytica): report from the SENTRY antimicrobial surveillance program. J Clin Microbiol 2009 Dec; 47(12): 41294130.

    • Search Google Scholar
    • Export Citation
  • 10.

    Tseng SP, Wang JT, Liang CY, Lee PS, Chen YC, Lu PL. First report of bla(IMP-8) in Raoultella planticola. Antimicrob Agents Chemother 2014; 58(1): 593595.

    • Search Google Scholar
    • Export Citation
  • 11.

    Tufa TB, Fuchs A, Feldt T, Galata DT, Mackenzie CR, Pfeffer K, et al. CTX-M-9 group ESBL-producing Raoultella planticola nosocomial infection: first report from sub-Saharan Africa. Ann Clin Microbiol Antimicrob 2020 Aug 17; 19(1): 36.

    • Search Google Scholar
    • Export Citation
  • 12.

    Li J, Lan R, Xiong Y, Ye C, Yuan M, Liu X, et al. Sequential isolation in a patient of Raoultella planticola and Escherichia coli bearing a novel ISCR1 element carrying blaNDM-1. PLoS One 2014 Mar 3; 9(3): e89893.

    • Search Google Scholar
    • Export Citation
  • 13.

    Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19(5): 455477.

    • Search Google Scholar
    • Export Citation
  • 14.

    Lee I, Ouk Kim Y, Park SC, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016 Feb; 66(2): 11001103.

    • Search Google Scholar
    • Export Citation
  • 15.

    Meier-Kolthoff JP, Carbasse JS, Peinado-Olarte RL, Göker M. TYGS and LPSN: a database tandem for fast and reliable genome-based classification and nomenclature of prokaryotes. Nucleic Acids Res 2022 Jan 7; 50(D1): D801d807.

    • Search Google Scholar
    • Export Citation
  • 16.

    Feldgarden M, Brover V, Haft DH, Prasad AB, Slotta DJ, Tolstoy I, et al. Validating the AMRFinder tool and resistance gene database by using antimicrobial resistance genotype-phenotype correlations in a collection of isolates. Antimicrob Agents Chemother 2019; 63: e00483-19.

    • Search Google Scholar
    • Export Citation
  • 17.

    Carattoli A, Zankari E, Garcia-Fernandez A, Voldby Larsen M, Lund O, Villa L, et al. PlasmidFinder and pMLST: in silico detection and typing of plasmids. Antimicrob Agents Chemother 2014; 58(7): 38953903.

    • Search Google Scholar
    • Export Citation
  • 18.

    Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35: 15471549.

    • Search Google Scholar
    • Export Citation
  • 19.

    Letunic I, Bork P. Interactive tree of life (iTOL) v4: recent updates and new developments. Nucleic Acids Res 2019; 47: W256W259.

  • 20.

    Drancourt M, Bollet C, Carta A, Rousselier P. Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov. Int J Syst Evol Microbiol 2001; 51: 925932.

    • Search Google Scholar
    • Export Citation
  • 21.

    Hong G, Yong HJ, Lee D, Kim DH, Kim YS, Park JS, et al. Clinical characteristics and treatment outcomes of patients with pneumonia caused by Raoultella planticola. J Thorac Dis 2020 Apr; 12(4): 13051311.

    • Search Google Scholar
    • Export Citation
  • 22.

    Kim SW, Kim JE, Hong YA, Ko GJ, Pyo HJ, Kwon YJ. Raoultella planticola peritonitis in a patient on continuous ambulatory peritoneal dialysis. Infection 2015; 43: 771775.

    • Search Google Scholar
    • Export Citation
  • 23.

    de Campos FP, Guimarães TB, Lovisolo SM. Fatal pancreatic pseudocyst co-infected by Raoultella planticola: an emerging pathogen. Autops Case Rep 2016; 6: 2731.

    • Search Google Scholar
    • Export Citation
  • 24.

    Puerta-Fernandez S, Miralles-Linares F, Sanchez-Simonet MV, Bernal-Lopez MR, Gomez-Huelgas R. Raoultella planticola bacteraemia secondary to gastroenteritis. Clin Microbiol Infect 2013; 19: E236E237.

    • Search Google Scholar
    • Export Citation
  • 25.

    Osterblad M, Kirveskari J, Hakanen AJ, Tissari P, Vaara M, Jalava J. Carbapenemase-producing Enterobacteriaceae in Finland: the first years (2008–11). J Antimicrob Chemother 2012; 67: 28602864.

    • Search Google Scholar
    • Export Citation
  • 26.

    Xu M, Xie W, Fu Y, Zhou H, Zhou J. Nosocomial pneumonia caused by carbapenem-resistant Raoultella planticola: a case report and literature review. Infection 2015 Apr; 43(2): 245248.

    • Search Google Scholar
    • Export Citation
  • Collapse
  • Expand

Senior editors

Editor-in-Chief: Prof. Dóra Szabó (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Managing Editor: Dr. Béla Kocsis (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Co-editor: Dr. Andrea Horváth (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)

Editorial Board

  • Prof. Éva ÁDÁM (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Sebastian AMYES (Department of Medical Microbiology, University of Edinburgh, Edinburgh, UK.)
  • Dr. Katalin BURIÁN (Institute of Clinical Microbiology University of Szeged, Szeged, Hungary; Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.)
  • Dr. Orsolya DOBAY (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Ildikó Rita DUNAY (Institute of Inflammation and Neurodegeneration, Medical Faculty, Otto-von-Guericke University, Magdeburg, Germany; Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany)
  • Prof. Levente EMŐDY(Department of Medical Microbiology and Immunology, University of Pécs, Pécs, Hungary.)
  • Prof. Anna ERDEI (Department of Immunology, Eötvös Loránd University, Budapest, Hungary, MTA-ELTE Immunology Research Group, Eötvös Loránd University, Budapest, Hungary.)
  • Prof. Éva Mária FENYŐ (Division of Medical Microbiology, University of Lund, Lund, Sweden)
  • Prof. László FODOR (Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary)
  • Prof. József KÓNYA (Department of Medical Microbiology, University of Debrecen, Debrecen, Hungary)
  • Prof. Yvette MÁNDI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Károly MÁRIALIGETI (Department of Microbiology, Eötvös Loránd University, Budapest, Hungary)
  • Prof. János MINÁROVITS (Department of Oral Biology and Experimental Dental Research, University of Szeged, Szeged, Hungary)
  • Prof. Béla NAGY (Centre for Agricultural Research, Institute for Veterinary Medical Research, Budapest, Hungary.)
  • Prof. István NÁSZ (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Kristóf NÉKÁM (Hospital of the Hospitaller Brothers in Buda, Budapest, Hungary.)
  • Dr. Eszter OSTORHÁZI (Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary)
  • Prof. Rozália PUSZTAI (Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary)
  • Prof. Peter L. RÁDY (Department of Dermatology, University of Texas, Houston, Texas, USA)
  • Prof. Éva RAJNAVÖLGYI (Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary)
  • Prof. Ferenc ROZGONYI (Institute of Laboratory Medicine, Semmelweis University, Budapest, Hungary)
  • Prof. Joseph G. SINKOVICS (The Cancer Institute, St. Joseph’s Hospital, Tampa, Florida, USA)
  • Prof. Júlia SZEKERES (Department of Medical Biology, University of Pécs, Pécs, Hungary.)
  • Prof. Mária TAKÁCS (National Reference Laboratory for Viral Zoonoses, National Public Health Center, Budapest, Hungary.)
  • Prof. Edit URBÁN (Department of Medical Microbiology and Immunology University of Pécs, Pécs, Hungary; Institute of Translational Medicine, University of Pécs, Pécs, Hungary.)

 

Editorial Office:
Akadémiai Kiadó Zrt.
Budafoki út 187-187, A/3, H-1117 Budapest, Hungary

Editorial Correspondence:
Acta Microbiologica et Immunologica Hungarica
Institute of Medical Microbiology
Semmelweis University
P.O. Box 370
H-1445 Budapest, Hungary
Phone: + 36 1 459 1500 ext. 56101
Fax: (36 1) 210 2959
E-mail: amih@med.semmelweis-univ.hu

 Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • CABELLS Journalytics
  • Chemical Abstracts
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • SCOPUS
  • Science Citation Index Expanded

2023  
Web of Science  
Journal Impact Factor 1.3
Rank by Impact Factor Q4 (Immunology)
Journal Citation Indicator 0.31
Scopus  
CiteScore 2.3
CiteScore rank Q3 (Infectious Diseases)
SNIP 0.389
Scimago  
SJR index 0.308
SJR Q rank Q3

Acta Microbiologica et Immunologica Hungarica
Publication Model Hybrid
Submission Fee none
Article Processing Charge 1100 EUR/article (only for OA publications)
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription fee 2025 Online subsscription: 772 EUR / 848 USD
Print + online subscription: 860 EUR / 944 USD
Subscription Information Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content.
Purchase per Title Individual articles are sold on the displayed price.

Acta Microbiologica et Immunologica Hungarica
Language English
Size A4
Year of
Foundation
1954
Volumes
per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 1217-8950 (Print)
ISSN 1588-2640 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
May 2024 103 0 0
Jun 2024 65 0 1
Jul 2024 59 0 0
Aug 2024 76 0 0
Sep 2024 123 0 0
Oct 2024 199 1 1
Nov 2024 36 0 0