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Abstract
The prevalence of Streptococcus agalactiae infections in adult populations is increasing. The current study aimed to characterize the genetic features of S. agalactiae strains responsible for different infections. A cross-sectional study was performed on 65 S. agalactiae strains (30 invasive and 35 noninvasive) isolated from non-pregnant women. All S. agalactiae isolates were confirmed by atr and dltS PCR assays. Antibiotic susceptibility patterns were determined using the disk diffusion method. Biofilm production was investigated by microtiter plate assay. PCR was done to detect resistance determinants. Isolates were characterized using the multilocus sequence typing (MLST) method. cMLSB, iMLSB, and M phenotypes accounted for 47.7%, 30.8%, and 6.2%, respectively. MDR was detected in 15.4% of noninvasive and 44.6% of invasive isolates. MtP assay indicated that 80% of isolates were biofilm producers. Biofilm formation was common among noninvasive compared with invasive strains (94.3% versus 66.7%). tet (M) (46.2%) and erm (B) (69.2%) were the most prevalent tetracycline and macrolide-resistance genes. The most prevalent serotype was type III (50.8%), followed by Ia (18.4%), II (15.4%), V (12.3%), and IV (3.1%). The frequency of serotype III among biofilm producer strains (81.8%) was found to be significantly higher than that of non-producer isolates (18.2%) (P < 0.05). S. agalactiae was resolved within four clonal complexes, including CC19 (46.2%; in both invasive and noninvasive), followed by CC23 (30.8%; only noninvasive isolates), CC1 (15.4%; only noninvasive isolates) and CC17 (7.6%; only invasive isolates). The main sequence types (STs) found were ST19 (27.7%), ST17 (7.7%), ST27 (6.2%), and ST28 (4.6%) linked with invasive infections and ST23 (18.4%), ST933 (12.3%), ST644 (9.2%), ST19 (7.7%), ST1 (6.2%) found in noninvasive infections. The high prevalence of CC19 and CC23 clones among S. agalactiae strains reflects the emergence of these lineages as successful clones in Iran.
-
1.
Shabayek S, Spellerberg B. Group B streptococcal colonization, molecular characteristics, and epidemiology. Front Microbiol 2018; 9: 437. https://doi.org/10.3389/fmicb.2018.00437.
-
2.
Bianchi-Jassir F, Paul P, To KN, Carreras-Abad C, Seale AC, Jauneikaite E, et al. A systematic review of Group B Streptococcal capsular types, sequence types, and surface proteins as potential vaccine candidates. Vaccine 2020; 38: 6682–6694. https://doi.org/10.1016/j.vaccine.2020.08.052.
-
3.
Raabe VN, Shane AL. Group B streptococcus (Streptococcus agalactiae). Microbiol Spectr 2019; 7: 7.2. 17. https://doi.org/10.1128/microbiolspec.GPP3-0007-2018.
-
4.
Gherardi G, Imperi M, Baldassarri L, Pataracchia M, Alfarone G, Recchia S, et al. Molecular epidemiology and distribution of serotypes, surface proteins, and antibiotic resistance among group B streptococci in Italy. J Clin Microbiol 2007; 45: 2909–2916. https://doi.org/10.1128/JCM.00999-07.
-
5.
Brzychczy-Wloch M, Gosiewski T, Bulanda M. Multilocus sequence types of invasive and colonizing neonatal group B streptococci in Poland. Med Princ Pract 2014; 23: 323-330. https://doi.org/10.1159/000362368.
-
6.
Cheng Z, Qu P, Ke P, Yang X, Zhou Q, Lan K, et al. Antibiotic resistance and molecular epidemiological characteristics of Streptococcus agalactiae isolated from pregnant women in Guangzhou, South China. Can J Infect Dis Med Microbiol 2020; 2020. https://doi.org/10.1155/2020/1368942.
-
7.
Ezhumalai M, Muthanna A, Suhaili Z, Dzaraly ND, Amin-Nordin S, Amal MN, et al. Multilocus sequence typing analysis of invasive and non-invasive Group B Streptococcus of hospital origin in Malaysia. Malays J Med Sci 2020; 27: 134. https://doi.org/10.21315/mjms2020.27.1.14.
-
8.
Arabestani MR, Mousavi SM, Nasaj M. Genotyping of clinical Streptococcus agalactiae strains based on molecular serotype of capsular (cps) gene cluster sequences using polymerase chain reaction. Arch Clin Infect Dis 2017; 12. https://doi.org/10.5812/archcid.36787.
-
9.
Goudarzi M, Mohammadi A, Amirpour A, Fazeli M, Nasiri MJ, Hashemi A, et al. Genetic diversity and biofilm formation analysis of Staphylococcus aureus causing urinary tract infections in Tehran, Iran. J Infect Dev Ctries 2019; 13: 777–785. https://doi.org/10.3855/jidc.11329.
-
10.
Yousefi M, Pourmand MR, Fallah F, Hashemi A, Mashhadi R, Nazari-Alam A. Characterization of Staphylococcus aureus biofilm formation in urinary tract infection. Iran J Public Health 2016; 45: 485.
-
11.
Navidinia M, Mohammadi A, Arjmand R, Dadashi M, Goudarzi M. Molecular typing, biofilm formation, and analysis of adhesion factors in Staphylococcus aureus strains isolated from urinary tract infections. Gene Rep 2021; 22: 101008. https://doi.org/10.1016/j.genrep.2020.101008.
-
12.
Palmeiro JK, Dalla-Costa LM, Fracalanzza SE, Botelho AC, da Silva Nogueira K, Scheffer MC, et al. Phenotypic and genotypic characterization of group B streptococcal isolates in southern Brazil. J Clin Microbiol 2010; 48: 4397–4403. https://doi.org/10.1128/JCM.00419-10.
-
13.
Maeda T, Tsuyuki Y, Fujita T, Fukushima Y, Goto M, Yoshida H, et al. Comparison with Streptococcus agalactiae isolates from humans reveals genotypic and phenotypic characteristics of isolates from companion animal-origin. Jpn J Infect Dis 2020: JJID. 2019.441.
-
14.
Kimura K, Suzuki S, Wachino JI, Kurokawa H, Yamane K, Shibata N, et al. First molecular characterization of group B streptococci with reduced penicillin susceptibility. Antimicrob Agents Chemother 2008; 52: 2890–2897. https://doi.org/10.1128/AAC.00185-08.
-
15.
Aboutorabi SS, Rasooli Z, Pakniat H, Baloo F. Molecular characterization of prevalence, serotypes, virulence factors, and antibiotic resistance of Streptococcus agalactiae in a maternity hospital, Iran. Integr Cancer Sci Ther 2021; 8: 1000356. https://doi.org/10.15761/ICST.1000356.
-
16.
Dahesh S, Hensler ME, Van Sorge NM, Gertz Jr RE, Schrag S, Nizet V, et al. A point mutation in the group B streptococcal pbp2x gene conferring decreased susceptibility to β-lactam antibiotics. Antimicrob Agents Chemother 2008; 52: 2915–2918. https://doi.org/10.1128/AAC.00461-08.
-
17.
Nakamura PA, Schuab RB, Neves FP, Pereira CF, de Paula GR, Barros RR. Antimicrobial resistance profiles and genetic characterization of macrolide-resistant isolates of Streptococcus agalactiae. Mem Inst Oswaldo Cruz 2011; 106: 119–122. https://doi.org/10.1590/S0074-02762011000200001.
-
18.
Gizachew M, Tiruneh M, Moges F, Adefris M, Tigabu Z, Tessema B. Molecular characterization of Streptococcus agalactiae isolated from pregnant women and newborns at the University of Gondar comprehensive specialized hospital, Northwest Ethiopia. BMC Infect Dis 2020; 20: 1–9. https://doi.org/10.1186/s12879-020-4776-7.
-
19.
Piccinelli G, Biscaro V, Gargiulo F, Caruso A, De Francesco MA. Characterization and antibiotic susceptibility of Streptococcus agalactiae isolates causing urinary tract infections. Infect Genet Evol 2015; 34: 1–6. https://doi.org/10.1016/j.meegid.2015.07.001.
-
20.
Emaneini M, Jabalameli F, Mirsalehian A, Ghasemi A, Beigverdi R. Characterization of virulence factors, antimicrobial resistance pattern and clonal complexes of group B streptococci isolated from neonates. Microb Pathog 2016; 99: 119–122. https://doi.org/10.1016/j.micpath.2016.08.016.
-
21.
Lu B, Chen X, Wang J, Wang D, Zeng J, Li Y, et al. Molecular characteristics and antimicrobial resistance in invasive and noninvasive Group B Streptococcus between 2008 and 2015 in China. Diagn Microbiol Infect Dis 2016; 86: 351–357. https://doi.org/10.1016/j.diagmicrobio.2016.08.023.
-
22.
Björnsdóttir ES, Martins ER, Erlendsdóttir H, Haraldsson G, Melo-Cristino J, Kristinsson KG, et al. Changing epidemiology of group B streptococcal infections among adults in Iceland: 1975–2014. Clin Microbiol Infect 2016; 22: 379. e9–e16. https://doi.org/10.1016/j.cmi.2015.11.020.
-
23.
Moltó-García B, del Carmen Liébana-Martos M, Cuadros-Moronta E, Rodríguez-Granger J, Sampedro-Martínez A, Rosa-Fraile M, et al. Molecular characterization and antimicrobial susceptibility of hemolytic Streptococcus agalactiae from post-menopausal women. Maturitas 2016; 85: 5–10. https://doi.org/10.1016/j.maturitas.2015.11.007.
-
24.
Zhang L, Ma L, Zhu L, Zhou XH, Xu LJ, Guo C, et al. Molecular characterization of pathogenic group B streptococcus from a tertiary hospital in Shanxi, China: high incidence of sequence type 10 strains in infants/pregnant women. J Microbiol Immunol Infect 2021; 54: 1094–1100. https://doi.org/10.1016/j.jmii.2020.07.018.
-
25.
Kao Y, Tsai MH, Lai MY, Chu SM, Huang HR, Chiang MC, et al. Emerging serotype III sequence type 17 group B streptococcus invasive infection in infants: the clinical characteristics and impacts on outcomes. BMC Infect Dis 2019; 19: 1–8. https://doi.org/10.1186/s12879-019-4177-y.
-
26.
Luan SL, Granlund M, Sellin M, Lagergård T, Spratt BG, Norgren M. Multilocus sequence typing of Swedish invasive group B streptococcus isolates indicates a neonatally associated genetic lineage and capsule switching. J Clin Microbiol 2005; 43: 3727–3733. https://doi.org/10.1128/JCM.43.8.3727-3733.2005.
-
27.
Springman AC, Lacher DW, Waymire EA, Wengert SL, Singh P, Zadoks RN, et al. Pilus distribution among lineages of group b streptococcus: an evolutionary and clinical perspective. BMC Microbiol 2014; 14: 1–11. https://doi.org/10.1186/1471-2180-14-159.
-
28.
Al Safadi R, Amor S, Hery-Arnaud G, Spellerberg B, Lanotte P, Mereghetti L, et al. Enhanced expression of lmb gene encoding laminin-binding protein in Streptococcus agalactiae strains harboring IS 1548 in the scpB-lmb intergenic region. PLoS One 2010; 5: e10794. https://doi.org/10.1371/journal.pone.0010794.
-
29.
Bohnsack JF, Whiting A, Gottschalk M, Dunn DM, Weiss R, Azimi PH, et al. Population structure of invasive and colonizing strains of Streptococcus agalactiae from six US Academic Centers neonates from 1995 to 1999. J Clin Microbiol. 2008; 46(4): 1285–1291. https://doi.org/10.1128/JCM.02105-07.
-
30.
Huber CA, McOdimba F, Pflueger V, Daubenberger CA, Revathi G. Characterization of invasive and colonizing isolates of Streptococcus agalactiae in East African adults. J Clin Microbiol 2011; 49: 3652–3655. https://doi.org/10.1128/JCM.01288-11.
-
31.
Straková L, Musílek M, Motlová J. Multilocus sequence types in Czech neonatal GBS strains from 2004 to 2008. Epidemiol Mikrobiol Imunol 2010; 59: 45–47.
-
32.
Morozumi M, Wajima T, Kuwata Y, Chiba N, Sunaoshi K, Sugita K, et al. Associations between capsular serotype, multilocus sequence type, and macrolide resistance in Streptococcus agalactiae isolates from Japanese infants with invasive infections. Epidemiol Infect 2014; 142: 812–819. https://doi.org/10.1017/S0950268813001647.
-
33.
Jiang H, Chen M, Li T, Liu H, Gong Y, Li M. Molecular characterization of Streptococcus agalactiae causing community-and hospital-acquired infections in Shanghai, China. Front Microbiol 2016; 7: 1308. https://doi.org/10.3389/fmicb.2016.01308.
-
34.
Flores AR, Galloway-Peña J, Sahasrabhojane P, Saldaña M, Yao H, Su X, et al. Sequence type 1 group B Streptococcus, an emerging cause of invasive adult disease, evolves by small genetic changes. Proc Natl Acad Sci 2015; 112: 6431–6436. https://doi.org/10.1073/pnas.1504725112.
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