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Omer Faruk Cetiner
Omer Faruk Cetiner
Istanbul Faculty of Medicine, Istanbul University, Istanbul , Türkiye
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Mehmet Hora
Mehmet Hora
Department of Bioinformatics Systems Biology, Institute of Health Sciences, Erciyes University, Kayseri , Türkiye
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Ibrahim Halil Kafadar
Ibrahim Halil Kafadar
Department of Orthopedics and Traumatology, Erciyes University, Kayseri , Türkiye
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Aysegul Ulu Kilic
Aysegul Ulu Kilic
Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Erciyes University, Kayseri , Türkiye
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Aycan Gundogdu
agundogdu@erciyes.edu.tr
https://orcid.org/0000-0003-2806-8464
Aycan Gundogdu
Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Erciyes University, Kayseri , Türkiye
Metagenomics Laboratory, Genome and Stem Cell Center (GenKok), Erciyes University,Kayseri , Türkiye
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Metagenomics Laboratory, Genome and Stem Cell Center (GenKok), Erciyes University,
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Abstract
Prosthetic joint infection (PJI) and aseptic loosening (AL) are common complications of total joint arthroplasty. An accumulation of evidence indicates the presence of microbial communities on prosthetic implants, but the overall microbial profile is unclear. In this study, we aimed to investigate the differences in the microbial composition of prosthetic implants obtained from PJI and AL patients using the 16S rRNA sequencing method. Patients who underwent revision hip, knee, or shoulder arthroplasty caused by PJI (n = 20) or AL (n = 10) were enrolled in the study. 16S rRNA sequencing targeting the V3–V4 region was performed on the microbial specimens collected from synovial fluid, periprosthetic deep-tissue, and biofilm during the revision surgery. The sequenced raw data were analysed for microbial composition and ecological and differential abundance analyses using bioinformatics tools. The AL group had relatively balanced and higher diversity, with Staphylococcus, Streptococcus, and Veillonella being prominent. In the PJI group, Staphylococcus and Pseudomonas were predominant, especially in deep-tissue samples and biofilm samples, respectively. The differential abundance analysis identified 15 and 2 distinctive taxa in the AL and PJI groups, respectively. Our findings provided preliminary insights supporting the existence of periprosthetic microbiota in orthopedic implants and explaining the differences in microbial composition between the AL and PJI groups.
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