View More View Less
  • 1 Department of Microbiology, Rasht, Iran
  • 2 Department of Microbiology, Tehran, Iran
  • 3 Department of Microbiology, Tehran, Iran
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $784.00

Abstract

Inflammatory bowel disease (IBD) comprises two major illnesses: Crohn's disease (CD) and ulcerative colitis (UC). Dextran sulfate sodium (DSS) mouse colitis model has been used in understanding the mechanism of IBD. This study was conducted to examine selected Lactobacillus spp. as potential IBD treatment in the DSS-induced animal model. Balb/c mice were used and colitis was induced by adding 5% dextran sodium sulfate into the drinking water for 8 days. Colon length, disease activity index (DAI) and histological analysis were measured as markers of inflammation in DSS colitis mice. The majority of the Lactobacillus species significantly prevented the shortening of the colon length compared with the DSS group. The DAI scores of mice were significantly reduced following usage of four Lactobacillus strains included: Lactobacillus plantarum 03 and 06, Lactobacillus brevis 02 and Lactobacillus rhamnosus 01. The histological analysis exhibited that oral administration of Lactobacillus strains had therapeutic effects on mice colitis. L. plantarum and L. brevis showed better therapeutic effect against DSS-induced acute colitis mice. The probiotic activities of these three isolates indicated that the probiotic effects were strain specific and none of these useful bacteria could exhibit all of the valued probiotic properties simultaneously.

  • 1.

    Sheil B, Shanahan F, O'mahony L. Probiotic effects on inflammatory bowel disease. J Nutr. 2007; 137: 819824.

  • 2.

    Ye Y, Pang Z, Chen W, Ju S, Zhou C. The epidemiology and risk factors of inflammatory bowel disease. Int J Clin Exp Med. 2015; 8: 2252922542.

    • Search Google Scholar
    • Export Citation
  • 3.

    Bassaganya-Riera J, Viladomiu M, Pedragosa M, De Simone C, Hontecillas R. Immunoregulatory mechanisms underlying prevention of colitis-associated colorectal cancer by probiotic bacteria. PloS One 2012; 7: e34676.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 4.

    Jacouton E, Chain F, Sokol H, Langella P, Bermudez-Humaran LG. Probiotic strain Lactobacillus casei Bl23 Prevents colitis-associated colorectal cancer. Front Immunol. 2017; 8: 1553.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 5.

    Orel L, Kamhi TT. Intestinal microbiota, probiotics and prebiotics in inflammatory bowel disease. World J Gastroenterol. 2014; 20: 1150511524.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 6.

    Liu YW, Su YW, Ong WK, Cheng TH, Tsai YC. Oral administration of Lactobacillus plantarum K68 ameliorates DSS-induced ulcerative colitis in BALB/c mice via the anti-inflammatory and immunomodulatory activities. Int J Immunopharmacol. 2011; 11: 21592166.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 7.

    Ao X, Zhang X, Shi L, Zhao K, Yu J, Dong L, . Identification of lactic acid bacteria in traditional fermented yak milk and evaluation of their application in fermented milk products. J Dairy Sci. 2012; 95: 10731084.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 8.

    Bibalan MH, Eshaghi M, Rohani M, Esghaei M, Darban-Sarokhalil D, Pourshafie MR, . Isolates of Lactobacillus plantarum and L. reuteri display greater antiproliferative and antipathogenic activity than other Lactobacillus isolates. J Med Microbiol. 2017; 66: 14161420.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 9.

    Bibalan MH, Eshaghi M, Rohani M, Pourshafie MR, Talebi M. Determination of Bacteriocin Genes and Antibacterial Activity of Lactobacillus Strains Isolated from Fecal of Healthy Individuals. Int J Mol Cell Med. 2017; 6: 5055.

    • Search Google Scholar
    • Export Citation
  • 10.

    Matsumoto S, Hara T, Hori T, Mitsuyama K, Nagaoka M, Tomiyasu N, . Probiotic Lactobacillus‐induced improvement in murine chronic inflammatory bowel disease is associated with the down‐regulation of pro‐inflammatory cytokines in lamina propria mononuclear cells. Clin Exp Immunol. 2005; 140: 417426.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 11.

    Hamamoto N, Maemura K, Hirata I, Murano M, Sasaki S, Katsu K. Inhibition of dextran sulphate sodium (DSS)-induced colitis in mice by intracolonically administered antibodies against adhesion molecules (endothelial leucocyte adhesion molecule-1 (ELAM-1) or intercellular adhesion molecule-1 (ICAM-1)). Clin Exp Immunol. 1999; 117: 462468.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 12.

    Cooper HS, Murthy S, Shah R, Sedergran D. Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest. 1993; 69: 238249.

    • Search Google Scholar
    • Export Citation
  • 13.

    Pandurangan AK, Mohebali N, Esa NM, Looi CY, Ismail S, Saadatdoust Z. Gallic acid suppresses inflammation in dextran sodium sulfate-induced colitis in mice: Possible mechanisms. Int J Immunopharmacol. 2015; 28: 10341043.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 14.

    Macfarlane S, Furrie E, Cummings JH, Macfarlane GT. Chemotaxonomic analysis of bacterial populations colonizing the rectal mucosa in patients with ulcerative colitis. Clin Infect Dis. 2004; 38: 16901699.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 15.

    Kokešová A, Frolova L, Kverka M, Sokol D, Rossmann P, Bartova J, . Oral administration of probiotic bacteria (E. coli Nissle, E. coli O83, Lactobacillus casei) influences the severity of dextran sodium sulfate-induced colitis in BALB/c mice. Folia Microbiol. 2006; 51: 478484.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 16.

    Cui Y, Wei H, Lu F, Liu X, Liu D, Gu L, . Different effects of three selected Lactobacillus strains in dextran sulfate sodium-induced colitis in BALB/c mice. PloS One 2016; 11: e0148241.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 17.

    Chen X, Zhao X, Wang H, Yang Z, Li J, Suo H. Prevent effects of Lactobacillus fermentum HY01 on dextran sulfate sodium-induced colitis in mice. Nutrients. 2017; 9: 545.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 18.

    Geier MS, Butler RN, Giffard PM, Howarth GS. Lactobacillus fermentum BR11, a potential new probiotic, alleviates symptoms of colitis induced by dextran sulfate sodium (DSS) in rats. Int J Food Microbiol. 2007; 114: 267274.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 19.

    Toumi R, Soufli I, Rafa H, Belkhelfa M, Biad A, Touil-Boukoffa C. Probiotic bacteria lactobacillus and bifidobacterium attenuate inflammation in dextran sulfate sodium-induced experimental colitis in mice. Int J Immunopathol Pharmacol. 2014; 27: 615627.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 20.

    Peran L, Sierra S, Comalada M, Lara-Villoslada F, Bailón E, Nieto A, . A comparative study of the preventative effects exerted by two probiotics, Lactobacillus reuteri and Lactobacillus fermentum, in the trinitrobenzenesulfonic acid model of rat colitis. Br J Nutr. 2007; 97: 96103.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 21.

    Hudcovic T, Štěpánková R, Kozakova H, Hrnčíř T, Tlaskalova-Hogenova H. Effects of monocolonization with Escherichia coli strains O6K13 and nissle 1917 on the development of experimentally induced acute and chronic intestinal inflammation in germ-free immunocompetent and immunodeficient mice. Folia Microbiol. 2007; 52: 618626.

    • Crossref
    • Search Google Scholar
    • Export Citation

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
May 2020 0 0 0
Jun 2020 0 78 33
Jul 2020 15 17 9
Aug 2020 33 0 0
Sep 2020 57 1 1
Oct 2020 51 0 0
Nov 2020 0 0 0