View More View Less
  • 1 Aix Marseille University, IRD, AP-HM, SSA, VITROME, Marseille, France
  • 2 IHU-Méditerranée Infection, Marseille, France
  • 3 Aix Marseille University, MEPHI, Marseille, France
  • 4 Family Medicine Department, Thai Binh University of Medicine and Pharmacy, Thai Binh, Viet Nam
  • 5 VITROME, Campus International IRD-UCAD de l'IRD, Dakar, Senegal
  • 6 Pneumology Department, Thai Binh University of Medicine and Pharmacy, Thai Binh, Viet Nam
  • 7 Aix Marseille University, Service des urgences CHU Hôpital Nord, Marseille, France
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $784.00

Abstract

We aimed to assess the prevalence of pathogenic bacteria and resistance genes in rectal samples collected among homeless persons in Marseille, France. In February 2014 we enrolled 114 sheltered homeless adults who completed questionnaires and had rectal samples collected. Eight types of enteric bacteria and 15 antibiotic resistance genes (ARGs) were sought by real-time polymerase chain reaction (qPCR) performed directly on rectal samples. ARG-positive samples were further tested by conventional PCR and sequencing. We evidenced a 17.5% prevalence of gastrointestinal symptoms, a 9.6% prevalence of enteric pathogenic bacteria carriage, including Escherichia coli pathotypes (8.7%) and Tropheryma whipplei (0.9%). Only 2 persons carried blaCTX-M-15 resistance genes (1.8%), while other genes, including carbapenemase-encoding genes and colistin-resistance genes, (mcr-1 to mcr-6, mcr-8) were not detected. Our results suggest that sheltered homeless persons in Marseille do not have a high risk of harbouring gastrointestinal antibiotic resistant bacteria.

  • 1.

    Hines JZ, Pinsent T, Rees K, Vines J, Bowen A, Hurd J, . Notes from the field: shigellosis outbreak among men who have sex with men and homeless persons – Oregon, 2015–2016. MMWR Morb Mortal Wkly Rep 2016; 65(31): 81213. http://doi.org/10.15585/mmwr.mm6531a5.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 2.

    Hines JZ, Jagger MA, Jeanne TL, West N, Winquist A, Robinson BF, . Heavy precipitation as a risk factor for shigellosis among homeless persons during an outbreak – Oregon, 2015–2016. J Infect 2018; 76(3): 2805. http://doi.org/10.1016/j.jinf.2017.11.010.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 3.

    Murti M, Louie K, Bigham M, Hoang LMN. Outbreak of shigellosis in a homeless shelter with healthcare worker transmission-British Columbia, April 2015. Infect Control Hosp Epidemiol 2015; 36(11): 13723. http://doi.org/10.1017/ice.2015.211.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 4.

    Capone D, Ferguson A, Gribble MO, Brown J. Open defecation sites, unmet sanitation needs, and potential sanitary risks in Atlanta, Georgia, 2017–2018. Am J Public Health 2018; 108(9): 123840. http://doi.org/10.2105/AJPH.2018.304531.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 5.

    Sheer TA, Coyle WJ. Gastrointestinal tuberculosis. Curr Gastroenterol Rep 2003; 5(4): 2738. http://doi.org/10.1007/s11894-003-0063-1.

  • 6.

    Doshani M, Weng M, Moore KL, Romero JR, Nelson NP. Recommendations of the Advisory committee on immunization practices for use of hepatitis a vaccine for persons experiencing homelessness. MMWR Morb Mortal Wkly Rep 2019; 68(6): 1536. http://doi.org/10.15585/mmwr.mm6806a6.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 7.

    Zenu S, Alemayehu E, Woldemichael K. Prevalence of intestinal parasitic infections and associated factors among street children in Jimma town; south West Ethiopia in 2019: a cross sectional study. BMC Public Health 2019; 19(1): 1731. http://doi.org/10.1186/s12889-019-8083-4.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 8.

    Moges F, Kebede Y, Kassu A, Degu G, Tiruneh M, Gedefaw M. Infection with HIV and intestinal parasites among street dwellers in Gondar city, northwest Ethiopia. Jpn J Infect Dis 2016; 59(6): 4003.

    • Search Google Scholar
    • Export Citation
  • 9.

    Gomes TC, Almeida MF, Miura LA, Granja J, Santos DV, Oliveira RM, . Helmintoses intestinais em população de rua da cidade do Rio de Janeiro [Intestinal helminthiasis in street population of Rio de Janeiro city]. Rev Soc Bras Med Trop 2002; 35(5): 5312. Portuguese. http://doi.org/10.1590/s0037-86822002000500019.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 10.

    Bass JL, Brennan P, Mehta KA, Kodzis S. Pediatric problems in a suburban shelter for homeless families. Pediatrics 1990; 85(1): 338. Erratum in: Pediatrics, 1990, 85(3): 369.

    • Search Google Scholar
    • Export Citation
  • 11.

    Munita JM, Arias CA. Mechanisms of antibiotic resistance. Microbiol Spectr 2016; 4: 2.

  • 12.

    Hadjadj L, Riziki T, Zhu Y, Li J, Diene SM, Rolain JM. Study of mcr-1 Gene-mediated colistin resistance in Enterobacteriaceae isolated from humans and animals in different countries. Genes 2017; 8: 394. http://doi.org/10.3390/genes8120394.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 13.

    Moremi N, Claus H, Vogel U, Mshana SE. Faecal carriage of CTX-M extended-spectrum beta-lactamase-producing Enterobacteriaceae among street children dwelling in Mwanza city, Tanzania. PLoS One 2017; 12(9): e0184592. http://doi.org/10.1371/journal.pone.0184592.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 14.

    Keita AK, Brouqui P, Badiaga S, Benkouiten S, Ratmanov P, Raoult D, . Tropheryma whipplei prevalence strongly suggests human transmission in homeless shelters. Int J Infect Dis 2013; 17(1): e678. http://doi.org/10.1016/j.ijid.2012.05.1033.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 15.

    Ly TDA, Dao TL, Hoang VT, Braunstein D, Brouqui P, Lagier JC, . Pattern of infections in French and migrant homeless hospitalised at Marseille infectious disease units, France: a retrospective study, 2017–2018. Trav Med Infect Dis 2020; 36: 101768. http://doi.org/10.1016/j.tmaid.2020.101768.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 16.

    Ly TDA, Hadjadj L, Hoang VT, Louni M, Dao TL, Badiaga S, . Low prevalence of resistance genes in sheltered homeless population in Marseille, France, 2014–2018. Infect Drug Resist 2019; 12: 113951. http://doi.org/10.2147/IDR.S202048.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 17.

    Dao TL, Hoang VT, Ly TDA, Magmoun A, Canard N, Drali T, . Infectious disease symptoms and microbial carriage among French medical students travelling abroad: a prospective study. Trav Med Infect Dis 2020; 34: 101548. http://doi.org/10.1016/j.tmaid.2019.101548.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 18.

    Menu E, Mary C, Toga I, Raoult D, Ranque S, Bittar F. Evaluation of two DNA extraction methods for the PCR-based detection of eukaryotic enteric pathogens in fecal samples. BMC Res Notes 2018; 11: 206. http://doi.org/10.1186/s13104-018-3300-2.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 19.

    Sow D, Parola P, Sylla K, Ndiaye M, Delaunay P, Halfon P, . Performance of real-time polymerase chain reaction assays for the detection of 20 enteric parasites in clinical samples from Senegal. Am J Trop Med Hyg 2017; 97: 17382. http://doi.org/10.4269/ajtmh.16-0781.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 20.

    AbuOun M, Stubberfield EJ, Duggett NA, Kirchner M, Dormer L, Nunez-Garcia J, . mcr-1 and mcr-2 variant genes identified in Moraxella species isolated from pigs in Great Britain from 2014 to 2015. J Antimicrob Chemother 2017; 72(10): 27459. Erratum in: J Antimicrob Chemother, 2018; 73(10): 2904. http://doi.org/10.1093/jac/dky272.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 21.

    Chabou S, Leangapichart T, Okdah L, Le-Page S, Hadjadj L, Rolain JM. Real-time quantitative PCR assay with taqman((R)) probe for rapid detection of mcr-1 plasmid-mediated colistin resistance. New Microbes New Infect 2016; 13: 714. http://doi.org/10.1016/j.nmni.2016.06.017.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 22.

    Dandachi I, Fayad E, Sleiman A, Daoud Z, Rolain JM. Dissemination of multidrug-resistant and mcr-1 gram-negative bacilli in broilers, farm workers, and the surrounding environment in Lebanon. Microb Drug Resist 2020; 26(4): 36877. http://doi.org/10.1089/mdr.2019.0137.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 23.

    Nabti LZ, Sahli F, Ngaiganam EP, Radji N, Mezaghcha W, Lupande-Mwenebitu D, . Development of real-time PCR assay allowed describing the first clinical Klebsiella pneumoniae isolate harboring plasmid-mediated colistin resistance mcr-8 gene in Algeria. J Glob Antimicrob Resist 2020; 20: 26671. http://doi.org/10.1016/j.jgar.2019.08.018.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 24.

    Roschanski N, Fischer J, Guerra B, Roesler U. Development of a multiplex real-time PCR for the rapid detection of the predominant beta-lactamase genes CTX-M, SHV, TEM and CIT-type AmpCs in Enterobacteriaceae. PLoS One 2014; 9(7): e100956. http://doi.org/10.1371/journal.pone.0100956.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 25.

    Yu Y, Ji S, Chen Y, Zhou W, Wei Z, Li L, . Resistance of strains producing extended-spectrum beta-lactamases and genotype distribution in China. J Infect 2007; 54(1): 537. http://doi.org/10.1016/j.jinf.2006.01.014.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 26.

    Strauß LM, Dahms C, Becker K, Kramer A, Kaase M, Mellmann A. Development and evaluation of a novel universal β-lactamase gene subtyping assay for blaSHV, blaTEM and blaCTX-M using clinical and livestock-associated Escherichia coli. J Antimicrob Chemother 2015; 70(3): 71015. http://doi.org/10.1093/jac/dku450.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 27.

    Gupta SK, Padmanabhan BR, Diene SM, Lopez-Rojas R, Kempf M, Landraud L, . ARG-ANNOT, a new bioinformatic tool to discover antibiotic resistance genes in bacterial genomes. Antimicrob Agents Chemother 2014; 58: 21220. http://doi.org/10.1128/AAC.01310-13.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 28.

    Sow D, Dogue F, Edouard S, Drali T, Prades S, Battery E, . Acquisition of enteric pathogens by pilgrims during the 2016 Hajj pilgrimage: a prospective cohort study. Trav Med Infect Dis 2018; 25: 2630. http://doi.org/10.1016/j.tmaid.2018.05.017.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 29.

    Larrosa-Haro A, Macias-Rosales R, Sánchez-Ramírez CA. Seasonal variation of enteropathogens in infants and preschoolers with acute diarrhea in western Mexico. J Pediatr Gastroenterol Nutr 2010; 51(4): 5346. http://doi.org/10.1097/MPG.0b013e3181df5b66.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 30.

    Larrosa-Haro A, Macias-Rosales R, Sánchez-Ramírez CA, Cortés-López MC, Aguilar-Benavides S. Seasonal variation in bacterial pathogens isolated from stool samples in Karachi, Pakistan. J Pak Med Assoc 2003; 53(3): 1259. http://doi.org/10.1097/MPG.0b013e3181df5b66.

    • Search Google Scholar
    • Export Citation
  • 31.

    Leangapichart T, Tissot-Dupont H, Raoult D, Memish ZA, Rolain JM, Gautret P. Risk factors for acquisition of CTX-M genes in pilgrims during Hajj 2013 and 2014. J Antimicrob Chemother 2017; 72(9): 262735. http://doi.org/10.1093/jac/dkx155.

    • Crossref
    • Search Google Scholar
    • Export Citation

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Oct 2020 0 0 0
Nov 2020 0 0 0
Dec 2020 0 0 0
Jan 2021 5 0 0
Feb 2021 34 1 1
Mar 2021 25 2 1
Apr 2021 11 0 0