Extracts of five Euphorbia species were studied for their activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Candida albicans. E. amygdaloides and E. helioscopia proved to be the most effective against them. Some plant metabolits were also examinated against the microorganisms: podophyllin and rhamnetin caused the greatest, b-sitosterine the smallest inhibition among them. Bacillus subtilis was the least sensitive among the studied microorganism in this study.