Concentration of carbon (C), oxygen (O), nitrogen (N), magnesium (Mg), aluminium (Al), silicon (Si), phosphorus (P), mercury (Hg), sulphur (S), lead (Pb), chlorine (Cl), potassium (K), cadmium (Cd), calcium (Ca), chromium (Cr), manganese (Mn), iron (Fe), cobalt (Co), copper (Cu) and zinc (Zn), in the form of weight percentage, in the needles of different age classes, current and previous year one, of two sites, Risnjak National Park and Donja Dobra (control), were measured by X-ray microanalysis (EDAX) in the Scanning Electron Microscope in silver fir trees (Abies alba Mill.). The analysis suggests that accumulation of elements, in both years, particularly that of heavy metals, appeared in polluted site, like Risnjak, in the higher amount, whereas in non polluted, like Donja Dobra, in the lower amount. Moreover, it seems likely that elements at the first sites were accumulated and contaminated in very young current year needles, while in non polluted in somewhat older, the previous year ones.