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  • 1 Department of Plant Physiology, Faculty of Natural Sciences, Comenius University Mlynská dolina B-2, SK-842 15 Bratislava, Slovak Republic
  • 2 Institute of Plant Genetics, Slovak Academy of Sciences Akademická 2, P. O. Box 39 A, SK-950 07 Nitra, Slovak Republic
  • 3 Department of Plant Physiology, Faculty of Natural Sciences, Comenius University Mlynská dolina B-2, SK-842 15 Bratislava, Slovak Republic
  • 4 Institute of Botany, Slovak Academy of Sciences Dúbravská cesta 14, SK-842 23 Bratislava, Slovak Republic
  • 5 Department of Plant Physiology, Faculty of Natural Sciences, Comenius University Mlynská dolina B-2, SK-842 15 Bratislava, Slovak Republic
  • 6 Institute of Botany, Slovak Academy of Sciences Dúbravská cesta 14, SK-842 23 Bratislava, Slovak Republic
  • 7 Department of Plant Physiology, Faculty of Natural Sciences, Comenius University Mlynská dolina B-2, SK-842 15 Bratislava, Slovak Republic
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The possibility of plant regeneration via direct somatic embryogenesis from leaf tissue of Drosera spathulata Labill. has been studied, using MS medium supplemented with various concentration of NAA and BAP. Scanning electron microscopy investigations in combination with histological analysis verified direct somatic embryogenesis by formation of globular, torpedo-shaped, heart-shaped and cotyledonary embryo like structures. Optimal somatic embryo formation occurred when leaves were pre-treated with low concentration (0.005 mg l-1) of 2,4-D within 6 h. Prolonged influence of 2,4-D to 24-48 h led to the expression of morphological and developmental abnormalities.

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