In vitro antimicrobial activity of Wrightia tinctoria leaf extracts against skin microorganisms

P. Kannan; B. Shanmugavadivu; C. Petchiammal; W. Hopper

doi:10.1556/abot.48.2006.3-4.7

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Acta Botanica Hungarica

Volume/Issue:: Volume 48: Issue 3-4

In vitro antimicrobial activity of Wrightia tinctoria leaf extracts against skin microorganisms

Authors:

P. Kannan

P. Kannan Centre for Biotechnology, SPIC Science Foundation 88 Mount Road, Chennai 600 032, India

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B. Shanmugavadivu

B. Shanmugavadivu Centre for Biotechnology, SPIC Science Foundation 88 Mount Road, Chennai 600 032, India

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C. Petchiammal

C. Petchiammal Department of Environmental Biotechnology, Manonmaniam Sundaranar University Tirunelveli 627012, India

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W. Hopper

W. Hopper Department of Biotechnology, SRM Institute of Science and Technology, Ramapuram Chennai 600 089, India

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Pages:: 323–329

Online Publication Date:: 28 Sep 2006

Publication Date:: 01 Sep 2006

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/abot.48.2006.3-4.7

Keywords:: antifungal; Wrightia tinctoria; antibacterial; minimum inhibitory concentration

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Wrightia tinctoria leaf hexane, methanol and ethanol extracts were screened against skin bacteria and dermatophytes by in vitro. The extracts were tested using agar dilution method and broth micro dilution method. Methanol and ethanol extracts showed antibacterial activity; the MIC was 0.5 mg/ml for Bacillus subtilis and Staphylococcus epidermidis; 0.25 mg/ml for Staphylococcus aureus. The hexane extract showed antifungal activity against Trichophyton rubrum and Trichophyton tonsurans at 2 mg/ml. The MIC of 2 mg/ml was observed for methanol extract against Trichophyton mentagrophytes and IC₅₀ (2 mg/ml) was determined for Trichophyton rubrum and Epidermophyton floccosum. To summarise Wrightia tinctoria leaves possessed potent antimicrobial properties against dermatophytic microbes. In particular, methanol and ethanol extracts were active against bacteria and hexane extract was active against dermatophytic fungi, suggesting that the active principles may be useful in the topical treatment of superficial skin infections.

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Acta Botanica Hungarica

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Acta Botanica Hungarica
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