Changes in the formation of an extracellular matrix surface network during early stages of indirect somatic embryogenesis in Drosera spathulata

A. Blehová; M. Bobák; J. Šamaj; E. Hlinková

doi:10.1556/abot.52.2010.1-2.3

Acta Botanica Hungarica

Volume/Issue:: Volume 52: Issue 1-2

Changes in the formation of an extracellular matrix surface network during early stages of indirect somatic embryogenesis in Drosera spathulata

Authors:

A. Blehová

A. Blehová Comenius University Department of Plant Physiology Mlynská dolina B-2 SK-842 15 Bratislava Slovak Republic

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M. Bobák

M. Bobák Comenius University Department of Plant Physiology Mlynská dolina B-2 SK-842 15 Bratislava Slovak Republic

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J. Šamaj

J. Šamaj Slovak Academy of Sciences Institute of Plant Genetics Akademická 2 P. O. Box 39 A SK-950 07 Nitra Slovak Republic

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E. Hlinková

E. Hlinková Comenius University Institute of Cell Biology Mlynská dolina B-1 SK-842 15 Bratislava Slovak Republic

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Pages:: 23–33

Online Publication Date:: 17 Mar 2010

Publication Date:: 01 Mar 2010

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/abot.52.2010.1-2.3

Keywords:: cell surface network; pectins; proteins; somatic embryogenesis; transmission and scanning electron microscopy

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Our attention was focused on the changes occurring in the cell surface network linked to the induction of embryogenic competence of pre- and pro-embryogenic stages of indirect somatic embryogenesis in the callus tissue of Drosera spathulata Labill., which originated from isolated leaves. This surface network forms as a distinct and compact layer on globular somatic embryos before formation of protoderm. Young protodermal cells had a typical furrowed surface, whilst mature protodermal cells were practically smooth. Embryogenic cells show changes in structural organisation and chemical composition of cell surface network, during different stage formation of somatic embryogenesis. Using SEM, TEM analysis and enzymatic digestion of proteins and pectins, we have shown that granular components, which represent pectic polysaccharides, were linked to induction and acquisition of embryogenic competence and pre-embryogenic stage. Fibrillar network was linked to proembryogenic stage of somatic embryogenesis. Our study has revealed that combined effect of protease and pectinase after 5 hours caused a complete disappearance and removal of the cell surface network.

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Acta Botanica Hungarica

Print ISSN:: 0236-6495

Online ISSN:: 1588-2578

Senior editors

Editor(s)-in-Chief: Zoltán BOTTA-DUKÁT
Honorary Editor(s)-in-Chief: Attila BORHIDI

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László LŐKÖS
László PEREGOVITS

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Gy. BORBÉLY (Debrecen)
A. ČARNY (Ljubljana)
A. CSERGŐ (Dublin)
B. CZÚCZ (Paris)
M. HÖHN (Budapest)
K. T. KISS (Budapest)
A. KUZEMKO (Uman)
Z. LOSOSOVÁ (Brno)
I. MÁTHÉ (Szeged)
E. MIHALIK (Szeged)
S. ORBÁN (Eger)
R. PÁL (Butte)
Gy. PINKE (Mosonmagyaróvár)
T. PÓCS (Eger)
K. PRACH (České Budejovice)
E. S. RAUSCHERT (Cleveland)
E. RUPRECHT (Cluj Napoca)
G. SRAMKÓ (Debrecen)
A. T. SZABÓ (Veszprém)
É. SZŐKE (Budapest)
B. TOKARSKA-GUZIK (Katowice)
B. TÓTHMÉRÉSZ (Debrecen)
P. TÖRÖK (Debrecen)

Botta-Dukát, Zoltán
E-mail: botta-dukat.zoltan@okologia.mta.hu

or

Lőkös, László
E-mail: acta@bot.nhmus.hu
Institute: Botanical Department, Hungarian Natural History Museum
Address: Könyves K. krt. 40. H-1097 Budapest, Hungary

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Acta Botanica Hungarica
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