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  • 1 Faculty Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
  • | 2 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
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The genus Geranium (Geraniaceae); with about 320 species throughout the temperate regions, is chemically characterised by the presence of tannins, flavonoids, anthocyanins and essential oils which interfere with the extraction of pure genomic DNA. It is necessary to optimise the extraction protocols to reduce the effects of the presence of these compounds to the lowest level.

The present study compares the plant genomic DNA extraction Kit (DNP™ Kit), CTAB DNA extraction method by Murray and Thompson and Sahu et al., from the extracting DNA point of view Geranium species. The results showed significant differences in DNA contents between the three methods. Quantity and quality of extracted genomic DNAs were compared by employing the spectrophotometer, Nano-Drop, agarose gel electrophoresis, and polymerase chain reaction (PCR) methods and molecular marker such as (ITS and trnL-F) and ISSR. The method of Sahu et al., provided the best results (200 ng/µL) in terms of quantity and quality of DNA, therefore, this method was taken and optimised for DNA extraction. Our results proposed that this method could be effective for plants with same polysaccharides, proteins and polyphenols components. The advantage of this method is that it omits the use of liquid nitrogen and toxic phenols which are expensive. The success of this method in obtaining high-quality genomic DNA has been demonstrated in the Geranium species group and the reliability of this method has been discussed.

 

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  • Gy. BORBÉLY (Debrecen)
  • A. ČARNY (Ljubljana)
  • A. CSERGŐ (Dublin)
  • B. CZÚCZ (Paris)
  • M. HÖHN (Budapest)
  • K. T. KISS (Budapest)
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  • I. MÁTHÉ (Szeged)
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  • Gy. PINKE (Mosonmagyaróvár)
  • T. PÓCS (Eger)
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  • E. RUPRECHT (Cluj Napoca)
  • G. SRAMKÓ (Debrecen)
  • A. T. SZABÓ (Veszprém)
  • É. SZŐKE (Budapest)
  • B. TOKARSKA-GUZIK (Katowice)
  • B. TÓTHMÉRÉSZ (Debrecen)
  • P. TÖRÖK (Debrecen)

Botta-Dukát, Zoltán
E-mail: botta-dukat.zoltan@okologia.mta.hu

or

Lőkös, László
E-mail: acta@bot.nhmus.hu
Institute: Botanical Department, Hungarian Natural History Museum
Address: Könyves K. krt. 40. H-1097 Budapest, Hungary

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2021  
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Scimago  
Scimago
H-index
23
Scimago
Journal Rank
0,392
Scimago Quartile Score Plant Science (Q2)
Ecology, Evolution, Behavior and Systematics (Q3)
Scopus  
Scopus
Cite Score
2,5
Scopus
CIte Score Rank
Plant Science 205/482 (Q2)
Ecology, Evolution, Behavior and Systematics 322/687 (Q2)
Scopus
SNIP
1,046

2020  
Scimago
H-index
19
Scimago
Journal Rank
0,417
Scimago
Quartile Score
Plant Science Q2
Ecology, Evolution, Behavior and Systematics Q3
Scopus
Cite Score
155/89=1,7
Scopus
Cite Score Rank
Plant Science 221/445 (Q2)
Ecology, Evolution, Behavior and Systematics 374/647 (Q3)
Scopus
SNIP
0,838
Scopus
Cites
260
Scopus
Documents
22
Days from submission to acceptance 127
Days from acceptance to publication 132
Acceptance
Rate
36%

 

2019  
Scimago
H-index
17
Scimago
Journal Rank
0,404
Scimago
Quartile Score
Plant Science Q2
Ecology, Evolution, Behavior and Systematics Q3
Scopus
Cite Score
164/91=1,8
Scopus
Cite Score Rank
Plant Science 209/431 (Q2)
Ecology, Evolution, Behavior and Systematics 358/629 (Q3)
Scopus
SNIP
0,699
Scopus
Cites
215
Scopus
Documents
23
Acceptance
Rate
30%

 

Acta Botanica Hungarica
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Acta Botanica Hungarica
Language English
French
German
Russian
Spanish
Size B5
Year of
Foundation
1954
Volumes
per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-6495 (Print)
ISSN 1588-2578 (Online)

 

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