Parietaria L. is a genus comprised of 20 species distributed throughout temperate and subtropical areas of the world. The leaf and pollen characters of four species of Parietaria from different locations in Iran were investigated with light and scanning electron microscopy, in order to assess their diagnostic significance to provide additional evidence on species delimitation. All pollen grains were small in size and found to be porate. Most of the pollen characters, such as shape and sculpturing of pollen grains, exine diameter, were overlapped between species and indicated limited taxonomic value. The pore and annulus diameter could be provided additional taxonomic characters that can be used for species delimitation in the genus. Moreover, the leaf epidermis provided new qualitative data (laminar hydathodes, bladder-based trichomes) that could delimit the species. The characters like twin stomata and laminar hydathode observed in P. officinalis were reported for the first time in this genus.
Ajello, L. (1941): Cytology and cellular interrelations of cystolith formation in Ficus elastica.–Amer. J. Bot. 28(7): 589–594.
Arnott, H. (1980): Carbonates in higher plants, mechanisms of biomineralization in animals and plants.–Tokai University Press, pp. 211–218.
Assaf, H. K., Nafady, A. M. and Kamel, M. S. (2016): Botanical investigation of the leaf and stem of Forsskaolea tenacissima Linn., family Urticaceae, growing in Egypt.–Int. J. Pharm. Sci. 1(1): 12–19.
Boufford, D. E. (1992): Urticaceae, nettle family.–J. Ariz. Nev. Acad. Sci. 26(1): 42–49.
Burn, M. J. and Mayle, F. E. (2008): Palynological differentiation between genera of the Moraceae family and implications for Amazonian palaeoecology.–Rev. Palaeobot. Palynol. 149: 187–201.
Carr, S. G., Milkovits, L. and Carr, D. J. (1971): Eucalypt phytoglyphs: the microanatomical features of the epidermis in relation to taxonomy.–Aust. J. Bot. 19: 173–190.
Choi, B., Ryu, J. and Jang, T. S. (2022): Can pollen exine ornamentation contribute to species delimitation in Korean Iris L. taxa (Iridaceae)?–Palynology 46(4): 1–9.
Christenhusz, M. J. and Byng, J. W. (2016): The number of known plants species in the world and its annual increase.–Phytotaxa 261: 201–217. doi:
Davis, P. H. and Heywood, V. H. (1963): Principle of angiosperms taxonomy. – Van Nostrand, Princeton, NJ.
Dopazo, A., Mendez, J. and Seijo, C. (2000): Aplicación de los estudios morfológicos y biométricos a la caracterización de tipospolınicos. – Primera Reunión de la Red de Investigación Aerobiológica de Galicia.
Erdtman, G. (1952): Pollen morphology and plant taxonomy: Angiosperms (Vol. 1).–Chronica Botanica, Waltham, Massachusetts.
Fahn, A. (1988): Secretory tissues in vascular plants.–New Phytol. 108: 229–257.
Fernández Honaine, M., de Rito, M. V. and Osterrieth, M. L. (2018): Análisis de los silicofitolitos presentes en especies de las familias Cannabaceae, Moraceae y Urticaceae del SE bonaerense y estudio comparativo de los cistolitos.–Bol. Soc. Argent. Bot. 53(2): 227–237.
Friis, I. (1993): Urticaceae. – In: Flowering plants. Dicotyledons. Springer, pp. 612–630.
Gangadhara, M. and Inamdar, J. (1977): Trichomes and stomata, and their taxonomic significance in the Urticales.–Plant Syst. Evol. 127: 121–137.
Gifford, E. M. and Foster, A. S. (1989): Morphology and evolution of vascular plants. 3rd edn. – W. Freeman, New York.
Hammer, Ø., Harper, D. and Ryan, P. (2001): PAST-palaeontological statistics, ver. 1.89.–Pal. Electron. 4: 1–9.
Hesse, M., Halbritter, H., Zetter, R., Weber, M., Buchner, R., Frosch-Radivo, A. and Ulrich, S. (2009): Guidelines pollen terminology: an illustrated handbook. – New York, Springer Wien.
Huang, T. (1972): Pollen flora of Taiwan. – Botany Department Press, National Taiwan Univ.
Jafari, Z. and Dehghan, M. (2012): Anatomical structure study of aerial organs in four populations of Urtica dioica L.–Acta Hortic. 964: 33–38.
Janighorban, M. (2001): Flora of Iran: Urticaceae. Vol. 36.–Research Institute of Forests and Rangelands.
Judd, W. S. and Olmstead, R. G. (2004): A survey of tricolpate (eudicot) phylogenetic relationships.–Amer. J. Bot. 91: 1627–1644.
Kadiri, A. B., Oboh, B. and Oha, C. (2011): Systematic value of foliar epidermal morphology in some taxa of the tribes: Urticeae and Parietariae of the West African Urticaceae.–Thaiszia J. Bot. Košice 21: 73–83.
Kadluczka, D., Sliwinska, E. and Grzebelus, E. (2022): Combining genome size and pollen morphology data to study species relationships in the genus Daucus (Apiaceae).–BMC Plant Biol. 22: 382.
Klimko, M. and Truchan, M. (2006): Morphological variability of the leaf epidermis in selected taxa of the genus Ficus L. (Moraceae) and its taxonomic implications.–Acta Soc. Bot. Pol. 75: 309–324.
Kohl, F. G. (1889): Anatomisch-physiologische Untersuchung der Kalksalze und Kieselsäure in der Pflanze: Ein Beitrag zur Kenntnis der Mineralstoffe im lebenden Pflanzenkörper. – éditeur non identifié.
Lersten, N. and Curtis, J. (1991): Laminar hydathodes in Urticaceae: survey of tribes and anatomical observations on Pilea pumila and Urtica dioica. –Plant Syst. Evol. 176: 179–203.
Metcalfe, C. R. (1960): Anatomy of the monocotyledons. 1. Gramineae Anatomy of the monocotyledons 1. Gramineae. – Clarendon Press, London.
Metcalfe, C. R. and Chalk, L. (1950): Anatomy of the dicotyledons: leaves, stem, and wood, in relation to taxonomy, with notes on economic uses anatomy of the dicotyledons: leaves, stem, and wood, in relation to taxonomy, with notes on economic uses. – Clarendon Press, London.
Metcalfe, C. R. and Chalk, L. (1979): Anatomy of dicotyledons. systematic anatomy of leaf and stem, with a brief history of the subject. 2nd ed., Vol. 1. – Clarendon Press, Oxford.
Mete, D., Şahin, A. A., Hamzaoğlu, E. and Pinar, N. M. (2021): Pollen morphology applied to species delimitation of Turkish Dianthus L. (Caryophyllaceae).–Palynology 45(4): 599–625.
Moore, P. D., Webb, J. A. and Collison, M. E. (1991): Pollen analysis. – Blackwell Scientific Publications.
Murugesan, K. and Dan, M. (2019): Intraspecific variability in foliar micro-morphology of Pellionia heyneana Wedd. (Urticaceae), from Western Ghats.–Phytomorphology: Int. J. Plant Morphol. 69: 101–112.
Patel, R. and Inamdar, J. (1971): Structure and ontogeny of normal and abnormal stomata in vegetative and floral organs of Physalis minima L.–Aust. J. Bot. 19: 85–97.
Patil, A. and Patil, D. (2011): Occurrence and significance of cystoliths in Acanthaceae.–Curr. Bot. 2: 4.
Perveen, A., Grafström, E. and El-Ghazaly, G. (2004): World pollen and spore flora 23. Malvaceae Adams. P.p. Subfamilies: Grewioideae, Tilioideae, Brownlowioideae.–Grana 43: 129–155.
Pole, M. (2010): Cuticle morphology of Australasian Sapindaceae.–Bot. J. Linn. Soc. 164: 264–292.
Punt, W. and Malotaux, M. (1984): Cannabaceae, Moraceae and Urticaceae. – In: Punt, W. and Clarke, G. C. S. (eds): The northwest European pollen flora, IV. Elsevier, Amsterdam.
Punt, W., Hoen, P., Blackmore, S., Nilsson, S. and Le Thomas, A. (2007): Glossary of pollen and spore terminology.–Rev. Palaeobot. Palynol. 143: 1–81.
Rodriguez-Damian, M., Cernadas, E., Formella, A., Fernández-Delgado, M. and De SaOtero, P. (2006): Automatic detection and classification of grains of pollen based on shape and texture.–IEEE Transactions on Systems, Man, and Cybernetics, Part C (Applications and Reviews) 36(4): 531–542.
Rost, T. L. (1969): Vascular pattern and hydathodes in leaves of Crassula argentea (Crassulaceae).–Bot. Gaz. 130: 267–270.
Scott, F. M. (1946): Cystoliths and plasmodesmata in Beloperone, Ficus, and Boehmeria.–Bot. Gaz. 107: 372–378.
Selim, M. A. F. (2020): Systematic study on some Urticaceae Juss. species from Egypt.–Taeck holmia 40: 112–129.
Setoguchi, H., Okazaki, M. and Suga, S. (1989): Calcification in higher plants with special reference to cystoliths. – In: Origin, evolution, and modern aspects of biomineralization in plants and animals. Springer, pp. 409–418.
Shokefun, E. O., Orijemie, E. A. and Ayodele, A. E. (2014): Foliar epidermal and pollen characters of some species in the genus Microcos Linn. in Nigeria.–Am. J. Plant Sci. 5(26): 3904–3913.
Solereder, H. (1908): Systematic anatomy of the dicotyledons: a handbook for laboratories of pure and applied botany. Vol. 2.–Clarendon Press.
Sorsa, P. and Huttunen, P. (1975): On the pollen morphology of the Urticaceae.–JSTOR, pp. 165–182.
Stace, C. A. (1984): The taxonomic importance of the leaf surface. – In: Heywood, V. H. and Moore, D. M. (eds): Current concepts in plant taxonomy. Systematic Association Special Volume, No. 25. Academic Press, London, pp. 67–94.
Stuessy, T. F. (2009): Plant taxonomy: the systematic evaluation of comparative data. – Columbia University Press.
Sultani, A. H. and Al-shemarri, K. A. (2016): Morphological study of the pollen grains of some species belong to genera Urtica L. and Parietaria L. (Urticaceae) in Iraq.–Iraqi J. Sci. 57: 871–877.
Ullah, F., Gao, Y. D., Zaman, W. and Gao, X. F. (2022): Pollen morphology of Rosa sericea complex and their taxonomic contribution.–Diversity 14(9): 705.
Ummu-Hani, B. and Noraini, T. (2013): The structure of cystoliths in selected taxa of the genus Ficus L. (Moraceae) in Peninsular Malaysia. – In: AIP Conference Proceedings, vol 1. American Institute of Physics, pp. 372–376.
Weddell, H. A. (1856): Monographie de la famille des Urticées. Vol. 9.–Gide et J. Baudry, Hugues Algernon Weddell.
Wodehouse, R. P. (1935): Pollen grains. Their structure, identification and significance in science and medicine. – McGraw-Hill.
Wu, Z. Y., Monro, A. K., Milne, R. I., Wang, H., Yi, T. S., Liu, J. and Li, D. Z. (2013): Molecular phylogeny of the nettle family (Urticaceae) inferred from multiple loci of three genomes and extensive generic sampling.–Mol. Phyl. Evol. 69: 814–827.