Acta Physiologica Hungarica
Volume/Issue:
Volume 101: Issue 4
Changes in antioxidant enzymes and lipid peroxidation in extensor digitorum longus muscles of streptozotocin-diabetic rats may contribute to muscle atrophy
Authors: Koji Nonaka 1 , S. Une 2 , N. Tatsuta 3 , K. Ito 1 , and J. Akiyama 3
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  • 1 Osaka Prefecture University Department of Physical Therapy 6-7-30 Habikino, Habikino Osaka 583-8555 Japan
  • 2 Shujitsu Junior College Department of Living Science Okayama Japan
  • 3 Kibi International University Research Institute of Health Welfare Takahashi Japan
DOI:
https://doi.org/10.1556/APhysiol.101.2014.007
Page Count:
421–428
Publication Date:
01 Dec 2014
Online Publication Date:
08 Sep 2014
Article Category:
Research Article
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We investigated muscle atrophy, major antioxidant enzymes and lipid peroxidation in the extensor digitorum longus (EDL, predominantly fast fibers) and soleus (predominantly slow fibers) muscle of streptozotocin-diabetic rats. Female Wistar rats were divided into a control (n = 5) and streptozotocin-induced diabetic group (n = 5). Eight weeks after diabetes induction the EDL and soleus muscles were removed and catalase (CAT), glutathione peroxidase (GPX) and superoxide dismutase activity (SOD), and thiobarbituric acid reactive substances (TBARS) levels measured. The CAT activity increased in both the EDL and soleus muscles of the diabetic rats (p < 0.01), whereas the GPX and SOD activities were increased only in the EDL muscle (p < 0.01 and p < 0.05). The TBARS levels were only increased in the EDL muscle of the diabetic rats (p < 0.01). Both muscles showed significant atrophy but the EDL muscle elicited the greatest atrophy. In conclusion, it appears that adaptive responses to oxidative stress were adequate in the soleus muscle, but not in the EDL muscle, of diabetic rats. Thus fast twitch muscle fibers may be more susceptible to oxidative stress than slow twitch muscle fibers and this may contribute to muscle atrophy under diabetic conditions.

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Cover Acta Physiologica Hungarica
Acta Physiologica Hungarica
Print ISSN:
0231-424X
Online ISSN:
1588-2683
Keywords:
antioxidant enzyme; extensor digitorum longus; lipid peroxidation; soleus; STZ-induced diabetes

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