Acta Physiologica Hungarica
Volume/Issue:
Volume 102: Issue 2
The effect of subchronic supplementation with folic acid on homocysteine induced seizures
Authors:
A Rasic-Markovic Institute of Medical Physiology “Richard Burian”, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

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B Rankov-Petrovic Institute of Medical Physiology “Richard Burian”, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

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D Hrncic Institute of Medical Physiology “Richard Burian”, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

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D Krstic Institute of Chemistry, School of Medicine, University of Belgrade, Belgrade, Serbia

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M Colovic Institute of Nuclear Sciences, “Vinča”, Belgrade, Serbia

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Dj Macut Institute of Endocrinology, Diabetes and Metabolic Diseases, Clinical Center Belgrade, Belgrade, Serbia

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D Djuric Institute of Medical Physiology “Richard Burian”, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

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Prof. Dr. Olivera Stanojlovic Institute of Medical Physiology “Richard Burian”, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

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 MD, PhD
Pages:
151–162
Online Publication Date:
Jun 2015
Publication Date:
01 Jun 2015
Article Category:
Research Article
DOI:
https://doi.org/10.1556/036.102.2015.2.6
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Influence of folic acid on the CNS is still unclear. Folate has a neuroprotective effect, while on the other hand excess folate can exacerbate seizures in epileptics. The aim of the present study was to examine the effect of subchronic administration of folic acid on behavioural and electroencephalographic (EEG) characteristics of DL homocysteine thiolactone induced seizures in adult rats. The activity of Na+/K+-ATPase and Mg2+-ATPase in different brain regions was investigated. Adult male Wistar rats were divided into groups: 1. Controls (C, 0.9% NaCl); 2. DL homocysteine-thiolactone 8.0 mmol/kg (H); 3. Subchronic supplementation with folic acid 5 mg/kg for 7 days (F) and 4. Subchronic supplementation with F + single dose of H (FH). Seizure behaviour was assessed by incidence, latency, number and intensity of seizure episodes. Seizure severity was described by a descriptive scale with grades 0–4. For EEG recordings, three gold-plated recording electrodes were implanted into the skull. Subchronic supplementation with folic acid did not affect seizure incidence, median number of seizure episodes and severity in FH, comparison with H (p > 0.05). The majority of seizure episodes in all groups were of grade 2. There were no significant differences in lethal outcomes at 24 h upon H injection in the FH vs. H group. The activity of Na+/K+-ATPase and Mg2+-ATPase was significantly increased in almost all examined structures in the FH vs. H group. Subchronic folic acid administration did not exacerbate H induced seizures and completely recovered the activity of ATPases.

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Acta Physiologica Hungarica
Print ISSN:
0231-424X
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Acta Physiologica Hungarica
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