Acta Physiologica Hungarica
Volume/Issue:
Volume 88: Issue 1
Comparative haemodynamic studies of resting and active skeletal muscle in anaesthetised rats: role of nitric oxide
Authors: Csilla Hably 1 , J. Vág 2 , and J. Bartha 3
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  • 1 Department of Physiology, Semmelweis University of Medicine H-1444 Budapest 8., P.O.Box 259, Puskin u. 9, Hungary
  • 2 Clinic of Conservative Dentistry, Semmelweis University of Medicine Budapest, Hungary
  • 3 Department of Physiology Budapest, Hungary
DOI:
https://doi.org/10.1556/aphysiol.88.2001.1.3
Page Count:
25–33
Publication Date:
01 Nov 2001
Online Publication Date:
22 Jul 2005
Article Category:
Research Article
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Our previous studies have indicated that nitric oxide takes part in the basal regulation of vascular tone in skeletal muscle. The purpose of this study was to investigate whether nitric oxide has a role in the active hyperaemic response of a working muscle in a resting subject. Haemodynamic effects of nitric oxide synthase (NOS) inhibition (L-NAME, 10 mg/kg/30 min iv. infusion) were determined simultaneously in the resting m. quadriceps femoris and in the working (breathing) m. rectus abdominis in anaesthetised rats ( 86Rb accumulation technique). L-NAME increased blood pressure and total peripheral resistance (TPR) while it decreased cardiac output. Blood flow (BF) decreased and vascular resistance (VR) increased both in resting (BF: 8.91±1.97®5.92±2.59 ml/min/100 g, p<0.05; VR: 106±29.9®212±113 R, p<0.01) and working (BF: 17.0±4.78®6.93±2.15 ml/min/100g, p<0.001; VR: 57.0±18.5®160±56.7 R, p<0.01) muscle following NOS inhibition, but the percentile change of BF was higher in the working muscle (59%) than in the resting one (34%, p0.001). There was a positive correlation between the cardiac output and the blood flow of the resting muscle with or without L-NAME administration, but blood flow of the working muscle failed to have any correlation with the cardiac output in control animals. However, L-NAME administration decreased both the cardiac output and the blood flow and similarly to the resting muscle a positive correlation was found. In conclusion, the haemodynamic effects of NOS inhibition are higher in working muscle than in the resting one: the nitric oxide may have important role in vasodilatation during muscle activity. _a

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Cover Acta Physiologica Hungarica
Acta Physiologica Hungarica
Print ISSN:
0231-424X
Online ISSN:
1588-2683
Keywords:
nitric oxide; nitric oxide; nitric oxide; muscle blood flow; resting and working skeletal muscle; vascular tone; blood pressure; total peripheral resistance; cardiac output; L-NAME

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