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  • 1 Department of Physiology Faculty of Animal Science University of Kaposvár H-7400 Kaposvár, Guba S. u. 40, Hungary
  • 2 Department of Agricultural Chemistry, University of Agriculture Gödöllő, Hungary; present address: Ceva-Phylaxia Co. Ltd., Budapest, Hungary
  • 3 Institut für Phytochemie, Fakultät für Naturwissenschaften, Universität Witten/Herdecke Germany
  • 4 6. Ashley Park North Aberdeen, Scotland, UK
  • 5 6. Ashley Park North Aberdeen, Scotland, UK
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A panel of orally administered lectins (100 mg/kg b.w.) of different binding specificities was tested for suppression of voluntary food consumption in prefasted rats. PHA isolectins (Phaseolus vulgaris) and RPA-I (Robinia pseudoacacia) were found to exert a marked and significant effect, but two other gut-binding lectins, i.e. SBA (Glycine max) and WGA (Triticum vulgare) and several non-binding lectins were ineffective. In cannulated rats PHA infused into the duodenum induced food suppression, i.e. binding of the lectin to the mouth or stomach was unnecessary. Suppression of food consumption lasted through the whole nocturnal feeding period, control (BSA) and experimental (PHA) curves of cumulative food consumption showed a V-like divergence. Suppression by PHA or RPA-I showed very similar time courses, but a long-lasting inhibition of gastric emptying was only observed in the RPA-treated animals. Intraperitoneally administered lectins suppressed food consumption much more effectively than the oral ones, whereas Galanthus nivalis agglutinin (GNA) had little or no effect. It is concluded that lectins can be used as effective tools for the modulation of food consumption and gastric emptying in experimental animals.

  • Bardocz S, Grant G, Ewen SWB, Duguid TJ, Brown DS, Englyst K, Pusztai A: Reversible effect of phytohaemagglutinin on the growth and metabolism of rat gastrointestinal tract. Gut 37, 353-360 (1995)

    'Reversible effect of phytohaemagglutinin on the growth and metabolism of rat gastrointestinal tract ' () 37 Gut : 353 -360.

    • Search Google Scholar
  • Blumberg S, Hildeheim J, Yariv J, Wilson KJ: The use of 1-amino-L-fucose bound to Septharose in the isolation of L-fucose-binding proteins. Biochim. Biophys. Acta 264,171---176 (1972)

    'The use of 1-amino-L-fucose bound to Septharose in the isolation of L-fucose-binding proteins ' () 264 Biochim. Biophys. Acta : 171 -176.

    • Search Google Scholar
  • Broekaert WF, Nsimba-Lubaki M, Peeters B, Peumans WJ: A lectin from elder (Sambucus nigra L.) bark. Biochem. J. 221,163-169 (1984)

    'A lectin from elder (Sambucus nigra L.) bark ' () 221 Biochem. J. : 163 -169.

  • Göckeritz W, Körner IJ, Kopp J, Bergmann J, Pfüller K, Eifler R, Pfüller U: Mistletoe lectins: Comparative studies on cytotoxicity, receptor binding and their effect on the cytosolic calcium content in human lymphocytes. In: Van Driessche E: Lectins. Biology, Biochemistry, Clinical Biochemistry. Proc. of the 10th International Lectin Meeting (Szeged, Hungary), 10, 345-353 (1994)

    'Mistletoe lectins: Comparative studies on cytotoxicity, receptor binding and their effect on the cytosolic calcium content in human lymphocytes ' , , .

    • Search Google Scholar
  • Herzig KH, Bardocz S, Grant G, Nustede R, Fölsch UR, Pusztai A: Red kidney bean lectin is a potent CCK releasing stimulus inducing pancreatic growth. Gut 41, 333-338 (1997)

    'Red kidney bean lectin is a potent CCK releasing stimulus inducing pancreatic growth ' () 41 Gut : 333 -338.

    • Search Google Scholar
  • Larue-Achagiotis C, Picard M, Louis-Sylvestre J: Feeding behavior in rats on a complete diet containing Concanavalin A. Reprod. Nutr. Dev. 32, 343-350 (1992)

    'Feeding behavior in rats on a complete diet containing Concanavalin A ' () 32 Reprod. Nutr. Dev. : 343 -350.

    • Search Google Scholar
  • Pusztai A (1991): Plant Lectins. Cambridge Univ. Press, Cambridge

    Plant Lectins , ().

  • Pusztai A, Grant G, Gelencser E, Ewen SWB, Pfüller U, Eifler R, Bardocz S: Effects of an orally administered mistletoe (type-2 RIP) lectin on growth, body composition, small intestinal structure, and insulin levels in young rats. Nutr. Biochem. 9, 31-36 (1997)

    'Effects of an orally administered mistletoe (type-2 RIP) lectin on growth, body composition, small intestinal structure, and insulin levels in young rats ' () 9 Nutr. Biochem. : 31 -36.

    • Search Google Scholar
  • Pusztai A, Watt WB, Stewart JC: A comprehensive scheme for the isolation of trypsin inhibitors and the agglutinin from soybean seeds. J. Agric. Food Chem. 39, 862-866 (1991)

    'A comprehensive scheme for the isolation of trypsin inhibitors and the agglutinin from soybean seeds ' () 39 J. Agric. Food Chem. : 862 -866.

    • Search Google Scholar
  • van Damme EJM, Allen AK, Peumans WJ.: Isolation and characterization of a lectin with exclusive specificity towards mannose from snowdrop (Galanthus nivalis) bulbs. FEBS Letters 215, 140-144 (1987)

    'Isolation and characterization of a lectin with exclusive specificity towards mannose from snowdrop (Galanthus nivalis) bulbs ' () 215 FEBS Letters : 140 -144.

    • Search Google Scholar
  • van Damme EJM, Barre A, Smeets K, Torrekens S, van Leuven F, Rougé P, Peumans WJ: The bark of Robinia pseudoacacia contains a complex mixture of lectins. Plant Physiol. 107, 833-843 (1995)

    'The bark of Robinia pseudoacacia contains a complex mixture of lectins ' () 107 Plant Physiol. : 833 -843.

    • Search Google Scholar
  • van Damme EJM, Peumans WJ, Pusztai A, Bardocz S, (1997): Handbook of Plant Lectins: Properties and Biomedical Applications. Wiley, Chichester

    Handbook of Plant Lectins: Properties and Biomedical Applications , ().

  • Vretblad P: Purification of lectins by biospecific affinity chromatography. Biochim. Biophys. Acta 434, 169-176 (1976)

    'Purification of lectins by biospecific affinity chromatography ' () 434 Biochim. Biophys. Acta : 169 -176.

    • Search Google Scholar
  • Wang JL, Becker JW, Reeke GN jr, Edelman GM: Favin, a crystalline lectin from Vicia faba. J. Mol. Biol. 88, 259-262 (1974)

    'Favin, a crystalline lectin from Vicia faba. ' () 88 J. Mol. Biol. : 259 -262.

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